Efeitos do treinamento aeróbio sobre sinais precoces do remodelamento do ventrículo esquerdo induzido pelo diabetes Mellitus experimental

Detalhes bibliográficos
Autor(a) principal: Silva, Flavio Santos da
Data de Publicação: 2014
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/jspui/handle/123456789/16741
Resumo: Our aim was to investigate the effects of an aerobic training program on adverse and early left ventricle (LV) remodeling, using an experimental model of short-term type 1 diabetes (T1D). Wistar rats were divided in 4 groups: sedentary control (SC), trained control (TC), sedentary diabetic (SD) and trained diabetic (TD). T1D was induced by streptozotocin (45 mg/kg). The training program consisted of 4 weeks running on a treadmill (13 m/min, 60 min/day, 5 days/week). At the end of the experiments, hearts were collected for analysis of morphology and transcriptional profile of LV, by focusing on its remodeling. Deaths were recorded during the 4-week period. We verified high mortality among animals of DS group, whereas it was significantly reduced in DT group. DS group also showed an increase in cross-sectional area of cardiomyocytes and fibrosis. TD group exhibited reduction in measures of cardiac trophism, but with respect to collagen content, it was similar to CS group. Analysis of gene expression related to cardiac remodeling revealed decreased expression of collagen I and III, as well as low expression of MMP-2 in DS group. TD group showed decreased levels of mRNA for MMP-9, and unchanged gene expression of MMP-2 when compared with the CS group. The expression of MMP-2 and TGF-1 were increased in CT group. The ratio between gene expression of collagen I and III was increased in the CT group and decreased in diabetic groups. These results establish early changes of the structure and transcriptional profile of LV myocardium. Moreover, they indicate that aerobic exercise training plays specific protection against mechanisms responsible for cardiac damage observed in T1D
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spelling Silva, Flavio Santos dahttp://lattes.cnpq.br/6673785180440540http://lattes.cnpq.br/6391134710454943Abreu, Bento João da Graça Azevedohttp://lattes.cnpq.br/1725848357337658Lago, Pedro Dalhttp://lattes.cnpq.br/9299796177039282Bruno, Selma Sousahttp://lattes.cnpq.br/4056770607573210Dias, Fernando Augusto Lavezzo2014-12-17T15:16:19Z2014-08-222014-12-17T15:16:19Z2014-02-03SILVA, Flavio Santos da. Efeitos do treinamento aeróbio sobre sinais precoces do remodelamento do ventrículo esquerdo induzido pelo diabetes Mellitus experimental. 2014. 63 f. Dissertação (Mestrado em Movimento e Saúde) - Universidade Federal do Rio Grande do Norte, Natal, 2014.https://repositorio.ufrn.br/jspui/handle/123456789/16741Our aim was to investigate the effects of an aerobic training program on adverse and early left ventricle (LV) remodeling, using an experimental model of short-term type 1 diabetes (T1D). Wistar rats were divided in 4 groups: sedentary control (SC), trained control (TC), sedentary diabetic (SD) and trained diabetic (TD). T1D was induced by streptozotocin (45 mg/kg). The training program consisted of 4 weeks running on a treadmill (13 m/min, 60 min/day, 5 days/week). At the end of the experiments, hearts were collected for analysis of morphology and transcriptional profile of LV, by focusing on its remodeling. Deaths were recorded during the 4-week period. We verified high mortality among animals of DS group, whereas it was significantly reduced in DT group. DS group also showed an increase in cross-sectional area of cardiomyocytes and fibrosis. TD group exhibited reduction in measures of cardiac trophism, but with respect to collagen content, it was similar to CS group. Analysis of gene expression related to cardiac remodeling revealed decreased expression of collagen I and III, as well as low expression of MMP-2 in DS group. TD group showed decreased levels of mRNA for MMP-9, and unchanged gene expression of MMP-2 when compared with the CS group. The expression of MMP-2 and TGF-1 were increased in CT group. The ratio between gene expression of collagen I and III was increased in the CT group and decreased in diabetic groups. These results establish early changes of the structure and transcriptional profile of LV myocardium. Moreover, they indicate that aerobic exercise training plays specific protection against mechanisms responsible for cardiac damage observed in T1DNosso objetivo foi investigar os efeitos de um programa de treinamento aeróbio sobre o remodelamento adverso e precoce do ventrículo esquerdo (VE), utilizando modelo experimental de curto prazo de diabetes tipo 1 (DM1). Ratos Wistar foram divididos em 4 grupos: controle sedentário (CS), controle treinado (CT), diabético sedentário (DS) e diabético treinado (DT). O DM1 foi induzido por estreptozotocina (45 mg/kg). O programa de treinamento consistiu em 4 semanas de corrida em esteira (13 m/min, 60 min/dia, 5 dias/semana). Ao fim dos experimentos, os corações foram coletados para analise da morfologia e do perfil transcricional do VE, com foco em seu remodelamento. Os óbitos foram registrados durante as 4 semanas. Verificamos alta mortalidade entre os animais do grupo DS, enquanto que esta foi significativamente reduzida no grupo DT. O grupo DS apresentou aumento na área de secção transversa dos cardiomiócitos e fibrose. O grupo DT exibiu redução das medidas de trofismo cardíaco, mas com relação ao conteúdo colágeno, foi similar ao grupo CS. As análises de expressão de genes ligados ao remodelamento cardíaco revelaram redução na expressão dos colágenos I e III, além de baixa expressão da MMP-2, no grupo DS. O grupo DT apresentou diminuição dos níveis de mRNA para MMP-9, e expressão gênica de MMP-2 inalterada, se comparado ao grupo CS. As expressões da MMP-2 e do TGF-1 foram aumentadas no grupo CT. A razão entre expressão gênica dos colágenos I e III mostrou-se elevada no grupo CT e reduzida nos grupos diabéticos. Esses resultados estabelecem alterações precoces da estrutura e do perfil transcricional do VE. Ainda, indicam que o treinamento aeróbio exerce proteção específica contra mecanismos responsáveis pelo dano cardíaco observado no DM1Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal do Rio Grande do NortePrograma de Pós-Graduação em FisioterapiaUFRNBRMovimento e SaúdeDiabetes Mellitus tipo 1. Miocárdio. Fibrose. Exercício aeróbico. RatosType 1 diabetes Mellitus. Myocardium. Fibrosis. Aerobic exercise. RatsCNPQ::CIENCIAS DA SAUDE::FISIOTERAPIA E TERAPIA OCUPACIONALEfeitos do treinamento aeróbio sobre sinais precoces do remodelamento do ventrículo esquerdo induzido pelo diabetes Mellitus experimentalinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALFlavioSS_DISSERT.pdfapplication/pdf1009224https://repositorio.ufrn.br/bitstream/123456789/16741/1/FlavioSS_DISSERT.pdf11ff4662d4d8985817935b34117dbf5fMD51TEXTFlavioSS_DISSERT.pdf.txtFlavioSS_DISSERT.pdf.txtExtracted texttext/plain92219https://repositorio.ufrn.br/bitstream/123456789/16741/6/FlavioSS_DISSERT.pdf.txt162a0e13a6ff3d0c2c86a73010fa314eMD56THUMBNAILFlavioSS_DISSERT.pdf.jpgFlavioSS_DISSERT.pdf.jpgIM Thumbnailimage/jpeg2950https://repositorio.ufrn.br/bitstream/123456789/16741/7/FlavioSS_DISSERT.pdf.jpg0ab2a4742a8d72587d93090abcb74165MD57123456789/167412017-11-04 04:32:05.076oai:https://repositorio.ufrn.br:123456789/16741Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2017-11-04T07:32:05Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.por.fl_str_mv Efeitos do treinamento aeróbio sobre sinais precoces do remodelamento do ventrículo esquerdo induzido pelo diabetes Mellitus experimental
title Efeitos do treinamento aeróbio sobre sinais precoces do remodelamento do ventrículo esquerdo induzido pelo diabetes Mellitus experimental
spellingShingle Efeitos do treinamento aeróbio sobre sinais precoces do remodelamento do ventrículo esquerdo induzido pelo diabetes Mellitus experimental
Silva, Flavio Santos da
Diabetes Mellitus tipo 1. Miocárdio. Fibrose. Exercício aeróbico. Ratos
Type 1 diabetes Mellitus. Myocardium. Fibrosis. Aerobic exercise. Rats
CNPQ::CIENCIAS DA SAUDE::FISIOTERAPIA E TERAPIA OCUPACIONAL
title_short Efeitos do treinamento aeróbio sobre sinais precoces do remodelamento do ventrículo esquerdo induzido pelo diabetes Mellitus experimental
title_full Efeitos do treinamento aeróbio sobre sinais precoces do remodelamento do ventrículo esquerdo induzido pelo diabetes Mellitus experimental
title_fullStr Efeitos do treinamento aeróbio sobre sinais precoces do remodelamento do ventrículo esquerdo induzido pelo diabetes Mellitus experimental
title_full_unstemmed Efeitos do treinamento aeróbio sobre sinais precoces do remodelamento do ventrículo esquerdo induzido pelo diabetes Mellitus experimental
title_sort Efeitos do treinamento aeróbio sobre sinais precoces do remodelamento do ventrículo esquerdo induzido pelo diabetes Mellitus experimental
author Silva, Flavio Santos da
author_facet Silva, Flavio Santos da
author_role author
dc.contributor.authorID.por.fl_str_mv
dc.contributor.authorLattes.por.fl_str_mv http://lattes.cnpq.br/6673785180440540
dc.contributor.advisorID.por.fl_str_mv
dc.contributor.advisorLattes.por.fl_str_mv http://lattes.cnpq.br/6391134710454943
dc.contributor.referees1.pt_BR.fl_str_mv Abreu, Bento João da Graça Azevedo
dc.contributor.referees1ID.por.fl_str_mv
dc.contributor.referees1Lattes.por.fl_str_mv http://lattes.cnpq.br/1725848357337658
dc.contributor.referees2.pt_BR.fl_str_mv Lago, Pedro Dal
dc.contributor.referees2ID.por.fl_str_mv
dc.contributor.referees2Lattes.por.fl_str_mv http://lattes.cnpq.br/9299796177039282
dc.contributor.referees3.pt_BR.fl_str_mv Bruno, Selma Sousa
dc.contributor.referees3ID.por.fl_str_mv
dc.contributor.referees3Lattes.por.fl_str_mv http://lattes.cnpq.br/4056770607573210
dc.contributor.author.fl_str_mv Silva, Flavio Santos da
dc.contributor.advisor1.fl_str_mv Dias, Fernando Augusto Lavezzo
contributor_str_mv Dias, Fernando Augusto Lavezzo
dc.subject.por.fl_str_mv Diabetes Mellitus tipo 1. Miocárdio. Fibrose. Exercício aeróbico. Ratos
topic Diabetes Mellitus tipo 1. Miocárdio. Fibrose. Exercício aeróbico. Ratos
Type 1 diabetes Mellitus. Myocardium. Fibrosis. Aerobic exercise. Rats
CNPQ::CIENCIAS DA SAUDE::FISIOTERAPIA E TERAPIA OCUPACIONAL
dc.subject.eng.fl_str_mv Type 1 diabetes Mellitus. Myocardium. Fibrosis. Aerobic exercise. Rats
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS DA SAUDE::FISIOTERAPIA E TERAPIA OCUPACIONAL
description Our aim was to investigate the effects of an aerobic training program on adverse and early left ventricle (LV) remodeling, using an experimental model of short-term type 1 diabetes (T1D). Wistar rats were divided in 4 groups: sedentary control (SC), trained control (TC), sedentary diabetic (SD) and trained diabetic (TD). T1D was induced by streptozotocin (45 mg/kg). The training program consisted of 4 weeks running on a treadmill (13 m/min, 60 min/day, 5 days/week). At the end of the experiments, hearts were collected for analysis of morphology and transcriptional profile of LV, by focusing on its remodeling. Deaths were recorded during the 4-week period. We verified high mortality among animals of DS group, whereas it was significantly reduced in DT group. DS group also showed an increase in cross-sectional area of cardiomyocytes and fibrosis. TD group exhibited reduction in measures of cardiac trophism, but with respect to collagen content, it was similar to CS group. Analysis of gene expression related to cardiac remodeling revealed decreased expression of collagen I and III, as well as low expression of MMP-2 in DS group. TD group showed decreased levels of mRNA for MMP-9, and unchanged gene expression of MMP-2 when compared with the CS group. The expression of MMP-2 and TGF-1 were increased in CT group. The ratio between gene expression of collagen I and III was increased in the CT group and decreased in diabetic groups. These results establish early changes of the structure and transcriptional profile of LV myocardium. Moreover, they indicate that aerobic exercise training plays specific protection against mechanisms responsible for cardiac damage observed in T1D
publishDate 2014
dc.date.accessioned.fl_str_mv 2014-12-17T15:16:19Z
dc.date.available.fl_str_mv 2014-08-22
2014-12-17T15:16:19Z
dc.date.issued.fl_str_mv 2014-02-03
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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dc.identifier.citation.fl_str_mv SILVA, Flavio Santos da. Efeitos do treinamento aeróbio sobre sinais precoces do remodelamento do ventrículo esquerdo induzido pelo diabetes Mellitus experimental. 2014. 63 f. Dissertação (Mestrado em Movimento e Saúde) - Universidade Federal do Rio Grande do Norte, Natal, 2014.
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/jspui/handle/123456789/16741
identifier_str_mv SILVA, Flavio Santos da. Efeitos do treinamento aeróbio sobre sinais precoces do remodelamento do ventrículo esquerdo induzido pelo diabetes Mellitus experimental. 2014. 63 f. Dissertação (Mestrado em Movimento e Saúde) - Universidade Federal do Rio Grande do Norte, Natal, 2014.
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dc.publisher.none.fl_str_mv Universidade Federal do Rio Grande do Norte
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Fisioterapia
dc.publisher.initials.fl_str_mv UFRN
dc.publisher.country.fl_str_mv BR
dc.publisher.department.fl_str_mv Movimento e Saúde
publisher.none.fl_str_mv Universidade Federal do Rio Grande do Norte
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