Evidence of progenitor cell lineage rerouting in the adult mouse hippocampus after status epilepticus
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/jspui/handle/123456789/30098 |
Resumo: | Cell lineage in the adult hippocampus comprises multipotent and neuron-committed progenitors. In the present work, we fate-mapped neuronal progenitors using Dcx-CreERT2 and CAG-CAT-EGFP double-transgenic mice (cDCX/EGFP). We show that 3 days after tamoxifen-mediated recombination in cDCX/EGFP adult mice, GFP+ cells in the dentate gyrus (DG) co-expresses DCX and about 6% of these cells are proliferative neuronal progenitors. After 30 days, 20% of GFP+ generated from these progenitors differentiate into GFAP+ astrocytes. Unilateral intrahippocampal administration of the chemoconvulsants kainic acid (KA) or pilocarpine (PL) triggered epileptiform discharges and led to a significant increase in the number of GFP+ cells in both ipsi and contralateral DG. However, while PL favored the differentiation of neurons in both ipsi- and contralateral sides, KA stimulated neurogenesis only in the contralateral side. In the ipsilateral side, KA injection led to an unexpected increase of astrogliogenesis in the Dcx-lineage. We also observed a small number of GFP+/GFAP+ cells displaying radial-glia morphology ipsilaterally 3 days after KA administration, suggesting that some Dcx-progenitors could regress to a multipotent stage. The boosted neurogenesis and astrogliogenesis observed in the Dcx-lineage following chemoconvulsants administration correlated, respectively, with preservation or degeneration of the parvalbuminergic plexus in the DG. Increased inflammatory response, by contrast, was observed both in the DG showing increased neurogenesis or astrogliogenesis. Altogether, our data support the view that cell lineage progression in the adult hippocampus is not unidirectional and could be modulated by local network activity and GABA-mediated signaling |
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Moura, Daniela Maria de SousaBrandão, Juliana AlvesLentini, CeliaHeinrich, ChristopheQueiroz, Claudio Marcos Teixeira deCosta, Marcos Romualdo2020-09-18T14:32:13Z2020-09-18T14:32:13Z2020-09-18MOURA, D. M. S.; BRANDÃO, J. A.; LENTINI, C.; HEINRICH, C.; QUEIROZ, C. M.; COSTA, M. R. Evidence of progenitor cell lineage rerouting in the adult mouse hippocampus after status epilepticus. Front. Neurosci., [S. L.], v. 14, p. 571315, set. 2020. doi: 10.3389/fnins.2020.571315. Disponível em: https://www.frontiersin.org/articles/10.3389/fnins.2020.571315/full. Acesso em: 18 set. 2020.https://repositorio.ufrn.br/jspui/handle/123456789/3009810.3389/fnins.2020.571315Frontiers in NeuroscienceAttribution 3.0 Brazilhttp://creativecommons.org/licenses/by/3.0/br/info:eu-repo/semantics/openAccessAdult hippocampusNeurogenesisAstrogliogenesisStatus epilepticusFate-specificationKainic acidPilocarpineGABAergic interneuronsEvidence of progenitor cell lineage rerouting in the adult mouse hippocampus after status epilepticusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCell lineage in the adult hippocampus comprises multipotent and neuron-committed progenitors. In the present work, we fate-mapped neuronal progenitors using Dcx-CreERT2 and CAG-CAT-EGFP double-transgenic mice (cDCX/EGFP). We show that 3 days after tamoxifen-mediated recombination in cDCX/EGFP adult mice, GFP+ cells in the dentate gyrus (DG) co-expresses DCX and about 6% of these cells are proliferative neuronal progenitors. After 30 days, 20% of GFP+ generated from these progenitors differentiate into GFAP+ astrocytes. Unilateral intrahippocampal administration of the chemoconvulsants kainic acid (KA) or pilocarpine (PL) triggered epileptiform discharges and led to a significant increase in the number of GFP+ cells in both ipsi and contralateral DG. However, while PL favored the differentiation of neurons in both ipsi- and contralateral sides, KA stimulated neurogenesis only in the contralateral side. In the ipsilateral side, KA injection led to an unexpected increase of astrogliogenesis in the Dcx-lineage. We also observed a small number of GFP+/GFAP+ cells displaying radial-glia morphology ipsilaterally 3 days after KA administration, suggesting that some Dcx-progenitors could regress to a multipotent stage. The boosted neurogenesis and astrogliogenesis observed in the Dcx-lineage following chemoconvulsants administration correlated, respectively, with preservation or degeneration of the parvalbuminergic plexus in the DG. Increased inflammatory response, by contrast, was observed both in the DG showing increased neurogenesis or astrogliogenesis. Altogether, our data support the view that cell lineage progression in the adult hippocampus is not unidirectional and could be modulated by local network activity and GABA-mediated signalingengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNLICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/30098/3/license.txte9597aa2854d128fd968be5edc8a28d9MD53ORIGINALEvidenceProgenitorCellLineage_Costa_2020.pdfEvidenceProgenitorCellLineage_Costa_2020.pdfEvidenceProgenitorCellLineage_Costa_2020application/pdf12598778https://repositorio.ufrn.br/bitstream/123456789/30098/1/EvidenceProgenitorCellLineage_Costa_2020.pdfcc99b26cf4c7b968a7d6efb4c9e1aa0bMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.ufrn.br/bitstream/123456789/30098/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD52TEXTEvidenceProgenitorCellLineage_Costa_2020.pdf.txtEvidenceProgenitorCellLineage_Costa_2020.pdf.txtExtracted texttext/plain83029https://repositorio.ufrn.br/bitstream/123456789/30098/4/EvidenceProgenitorCellLineage_Costa_2020.pdf.txte66106a448f64e6ff947a89e51c9ce9bMD54THUMBNAILEvidenceProgenitorCellLineage_Costa_2020.pdf.jpgEvidenceProgenitorCellLineage_Costa_2020.pdf.jpgGenerated Thumbnailimage/jpeg1665https://repositorio.ufrn.br/bitstream/123456789/30098/5/EvidenceProgenitorCellLineage_Costa_2020.pdf.jpg3141e6b558e5029c9ab37338a1c9f9eeMD55123456789/300982020-09-20 04:50:55.285oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2020-09-20T07:50:55Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
Evidence of progenitor cell lineage rerouting in the adult mouse hippocampus after status epilepticus |
title |
Evidence of progenitor cell lineage rerouting in the adult mouse hippocampus after status epilepticus |
spellingShingle |
Evidence of progenitor cell lineage rerouting in the adult mouse hippocampus after status epilepticus Moura, Daniela Maria de Sousa Adult hippocampus Neurogenesis Astrogliogenesis Status epilepticus Fate-specification Kainic acid Pilocarpine GABAergic interneurons |
title_short |
Evidence of progenitor cell lineage rerouting in the adult mouse hippocampus after status epilepticus |
title_full |
Evidence of progenitor cell lineage rerouting in the adult mouse hippocampus after status epilepticus |
title_fullStr |
Evidence of progenitor cell lineage rerouting in the adult mouse hippocampus after status epilepticus |
title_full_unstemmed |
Evidence of progenitor cell lineage rerouting in the adult mouse hippocampus after status epilepticus |
title_sort |
Evidence of progenitor cell lineage rerouting in the adult mouse hippocampus after status epilepticus |
author |
Moura, Daniela Maria de Sousa |
author_facet |
Moura, Daniela Maria de Sousa Brandão, Juliana Alves Lentini, Celia Heinrich, Christophe Queiroz, Claudio Marcos Teixeira de Costa, Marcos Romualdo |
author_role |
author |
author2 |
Brandão, Juliana Alves Lentini, Celia Heinrich, Christophe Queiroz, Claudio Marcos Teixeira de Costa, Marcos Romualdo |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Moura, Daniela Maria de Sousa Brandão, Juliana Alves Lentini, Celia Heinrich, Christophe Queiroz, Claudio Marcos Teixeira de Costa, Marcos Romualdo |
dc.subject.por.fl_str_mv |
Adult hippocampus Neurogenesis Astrogliogenesis Status epilepticus Fate-specification Kainic acid Pilocarpine GABAergic interneurons |
topic |
Adult hippocampus Neurogenesis Astrogliogenesis Status epilepticus Fate-specification Kainic acid Pilocarpine GABAergic interneurons |
description |
Cell lineage in the adult hippocampus comprises multipotent and neuron-committed progenitors. In the present work, we fate-mapped neuronal progenitors using Dcx-CreERT2 and CAG-CAT-EGFP double-transgenic mice (cDCX/EGFP). We show that 3 days after tamoxifen-mediated recombination in cDCX/EGFP adult mice, GFP+ cells in the dentate gyrus (DG) co-expresses DCX and about 6% of these cells are proliferative neuronal progenitors. After 30 days, 20% of GFP+ generated from these progenitors differentiate into GFAP+ astrocytes. Unilateral intrahippocampal administration of the chemoconvulsants kainic acid (KA) or pilocarpine (PL) triggered epileptiform discharges and led to a significant increase in the number of GFP+ cells in both ipsi and contralateral DG. However, while PL favored the differentiation of neurons in both ipsi- and contralateral sides, KA stimulated neurogenesis only in the contralateral side. In the ipsilateral side, KA injection led to an unexpected increase of astrogliogenesis in the Dcx-lineage. We also observed a small number of GFP+/GFAP+ cells displaying radial-glia morphology ipsilaterally 3 days after KA administration, suggesting that some Dcx-progenitors could regress to a multipotent stage. The boosted neurogenesis and astrogliogenesis observed in the Dcx-lineage following chemoconvulsants administration correlated, respectively, with preservation or degeneration of the parvalbuminergic plexus in the DG. Increased inflammatory response, by contrast, was observed both in the DG showing increased neurogenesis or astrogliogenesis. Altogether, our data support the view that cell lineage progression in the adult hippocampus is not unidirectional and could be modulated by local network activity and GABA-mediated signaling |
publishDate |
2020 |
dc.date.accessioned.fl_str_mv |
2020-09-18T14:32:13Z |
dc.date.available.fl_str_mv |
2020-09-18T14:32:13Z |
dc.date.issued.fl_str_mv |
2020-09-18 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MOURA, D. M. S.; BRANDÃO, J. A.; LENTINI, C.; HEINRICH, C.; QUEIROZ, C. M.; COSTA, M. R. Evidence of progenitor cell lineage rerouting in the adult mouse hippocampus after status epilepticus. Front. Neurosci., [S. L.], v. 14, p. 571315, set. 2020. doi: 10.3389/fnins.2020.571315. Disponível em: https://www.frontiersin.org/articles/10.3389/fnins.2020.571315/full. Acesso em: 18 set. 2020. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/jspui/handle/123456789/30098 |
dc.identifier.doi.none.fl_str_mv |
10.3389/fnins.2020.571315 |
identifier_str_mv |
MOURA, D. M. S.; BRANDÃO, J. A.; LENTINI, C.; HEINRICH, C.; QUEIROZ, C. M.; COSTA, M. R. Evidence of progenitor cell lineage rerouting in the adult mouse hippocampus after status epilepticus. Front. Neurosci., [S. L.], v. 14, p. 571315, set. 2020. doi: 10.3389/fnins.2020.571315. Disponível em: https://www.frontiersin.org/articles/10.3389/fnins.2020.571315/full. Acesso em: 18 set. 2020. 10.3389/fnins.2020.571315 |
url |
https://repositorio.ufrn.br/jspui/handle/123456789/30098 |
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eng |
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eng |
dc.rights.driver.fl_str_mv |
Attribution 3.0 Brazil http://creativecommons.org/licenses/by/3.0/br/ info:eu-repo/semantics/openAccess |
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Attribution 3.0 Brazil http://creativecommons.org/licenses/by/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Frontiers in Neuroscience |
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Frontiers in Neuroscience |
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