Biochemical characterisation of a kunitz-type inhibitor from tamarindus indica L. seeds and its efficacy in reducing plasma leptin in an experimental model of obesity

Detalhes bibliográficos
Autor(a) principal: Maciel, Bruna Leal Lima
Data de Publicação: 2018
Outros Autores: Medeiros, Amanda Fernandes de, Costa, Izael de Sousa, Carvalho, Fabiana Maria Coimbra de, Kiyota, Sumika, Souza, Beatriz Blenda Pinheiro de, Sifuentes, Daniel Nogoceke, Serquiz, Raphael Paschoal, Uchôa, Adriana Ferreira, Santos, Elizeu Antunes dos, Morais, Ana Heloneida de Araújo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/handle/123456789/57470
http://dx.doi.org/10.1080/14756366.2017.1419220
Resumo: A trypsin inhibitor isolated from tamarind seed (TTI) has satietogenic effects in animals, increasing the cholecystokinin (CCK) in eutrophy and reducing leptin in obesity. We purified TTI (pTTI), characterised, and observed its effect upon CCK and leptin in obese Wistar rats. By HPLC, and after amplification of resolution, two protein fractions were observed: Fr1 and Fr2, with average mass of [M þ 14H]þ ¼ 19,594,690 Da and [M þ 13H]þ ¼ 19,578,266 Da, respectively. The protein fractions showed 54 and 53 amino acid residues with the same sequence. pTTI presented resistance to temperature and pH variations; IC50 was 2.7 1010mol.L1 and Ki was 2.9 1011mol.L1 . The 2-DE revealed spots with isoelectric points between pH 5 and 6, and one near pH 8. pTTI action on leptin decrease was confirmed. We conclude that pTTI is a Kunitz trypsin inhibitor with possible biotechnological health-related application
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spelling Maciel, Bruna Leal LimaMedeiros, Amanda Fernandes deCosta, Izael de SousaCarvalho, Fabiana Maria Coimbra deKiyota, SumikaSouza, Beatriz Blenda Pinheiro deSifuentes, Daniel NogocekeSerquiz, Raphael PaschoalUchôa, Adriana FerreiraSantos, Elizeu Antunes dosMorais, Ana Heloneida de Araújo2024-02-01T16:00:58Z2024-02-01T16:00:58Z2018-01MEDEIROS, Amanda Fernandes de; COSTA, Izael de Sousa; CARVALHO, Fabiana Maria Coimbra de; KIYOTA, Sumika; SOUZA, Beatriz Blenda Pinheiro de; SIFUENTES, Daniel Nogoceke; SERQUIZ, Raphael Paschoal; MACIEL, Bruna Leal Lima; UCHÔA, Adriana Ferreira; SANTOS, Elizeu Antunes dos; MORAIS, Ana Heloneida de Araújo. Biochemical characterisation of a kunitz-type inhibitor from tamarindus indica L. seeds and its efficacy in reducing plasma leptin in an experimental model of obesity. Journal Of Enzyme Inhibition And Medicinal Chemistry, [S.l.], v. 33, n. 1, p. 334-348, 1 jan. 2018. DOI: 10.1080/14756366.2017.1419220. Disponível em: https://www.tandfonline.com/doi/full/10.1080/14756366.2017.1419220. Acesso em: 30 jan. 2024.https://repositorio.ufrn.br/handle/123456789/57470http://dx.doi.org/10.1080/14756366.2017.1419220Journal of Enzyme Inhibition and Medicinal ChemistryAttribution 3.0 Brazilhttp://creativecommons.org/licenses/by/3.0/br/info:eu-repo/semantics/openAccessTamarindAntitrypticWistar ratsCCKBiochemical characterisation of a kunitz-type inhibitor from tamarindus indica L. seeds and its efficacy in reducing plasma leptin in an experimental model of obesityinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleA trypsin inhibitor isolated from tamarind seed (TTI) has satietogenic effects in animals, increasing the cholecystokinin (CCK) in eutrophy and reducing leptin in obesity. We purified TTI (pTTI), characterised, and observed its effect upon CCK and leptin in obese Wistar rats. By HPLC, and after amplification of resolution, two protein fractions were observed: Fr1 and Fr2, with average mass of [M þ 14H]þ ¼ 19,594,690 Da and [M þ 13H]þ ¼ 19,578,266 Da, respectively. The protein fractions showed 54 and 53 amino acid residues with the same sequence. pTTI presented resistance to temperature and pH variations; IC50 was 2.7 1010mol.L1 and Ki was 2.9 1011mol.L1 . The 2-DE revealed spots with isoelectric points between pH 5 and 6, and one near pH 8. pTTI action on leptin decrease was confirmed. We conclude that pTTI is a Kunitz trypsin inhibitor with possible biotechnological health-related applicationengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALBiochemicalCharacterisation_Medeiros_2018.pdfBiochemicalCharacterisation_Medeiros_2018.pdfapplication/pdf4923412https://repositorio.ufrn.br/bitstream/123456789/57470/1/BiochemicalCharacterisation_Medeiros_2018.pdf4110bc7c23d549bed4693644de89a258MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.ufrn.br/bitstream/123456789/57470/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/57470/3/license.txte9597aa2854d128fd968be5edc8a28d9MD53123456789/574702024-02-01 13:00:59.081oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2024-02-01T16:00:59Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.pt_BR.fl_str_mv Biochemical characterisation of a kunitz-type inhibitor from tamarindus indica L. seeds and its efficacy in reducing plasma leptin in an experimental model of obesity
title Biochemical characterisation of a kunitz-type inhibitor from tamarindus indica L. seeds and its efficacy in reducing plasma leptin in an experimental model of obesity
spellingShingle Biochemical characterisation of a kunitz-type inhibitor from tamarindus indica L. seeds and its efficacy in reducing plasma leptin in an experimental model of obesity
Maciel, Bruna Leal Lima
Tamarind
Antitryptic
Wistar rats
CCK
title_short Biochemical characterisation of a kunitz-type inhibitor from tamarindus indica L. seeds and its efficacy in reducing plasma leptin in an experimental model of obesity
title_full Biochemical characterisation of a kunitz-type inhibitor from tamarindus indica L. seeds and its efficacy in reducing plasma leptin in an experimental model of obesity
title_fullStr Biochemical characterisation of a kunitz-type inhibitor from tamarindus indica L. seeds and its efficacy in reducing plasma leptin in an experimental model of obesity
title_full_unstemmed Biochemical characterisation of a kunitz-type inhibitor from tamarindus indica L. seeds and its efficacy in reducing plasma leptin in an experimental model of obesity
title_sort Biochemical characterisation of a kunitz-type inhibitor from tamarindus indica L. seeds and its efficacy in reducing plasma leptin in an experimental model of obesity
author Maciel, Bruna Leal Lima
author_facet Maciel, Bruna Leal Lima
Medeiros, Amanda Fernandes de
Costa, Izael de Sousa
Carvalho, Fabiana Maria Coimbra de
Kiyota, Sumika
Souza, Beatriz Blenda Pinheiro de
Sifuentes, Daniel Nogoceke
Serquiz, Raphael Paschoal
Uchôa, Adriana Ferreira
Santos, Elizeu Antunes dos
Morais, Ana Heloneida de Araújo
author_role author
author2 Medeiros, Amanda Fernandes de
Costa, Izael de Sousa
Carvalho, Fabiana Maria Coimbra de
Kiyota, Sumika
Souza, Beatriz Blenda Pinheiro de
Sifuentes, Daniel Nogoceke
Serquiz, Raphael Paschoal
Uchôa, Adriana Ferreira
Santos, Elizeu Antunes dos
Morais, Ana Heloneida de Araújo
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Maciel, Bruna Leal Lima
Medeiros, Amanda Fernandes de
Costa, Izael de Sousa
Carvalho, Fabiana Maria Coimbra de
Kiyota, Sumika
Souza, Beatriz Blenda Pinheiro de
Sifuentes, Daniel Nogoceke
Serquiz, Raphael Paschoal
Uchôa, Adriana Ferreira
Santos, Elizeu Antunes dos
Morais, Ana Heloneida de Araújo
dc.subject.por.fl_str_mv Tamarind
Antitryptic
Wistar rats
CCK
topic Tamarind
Antitryptic
Wistar rats
CCK
description A trypsin inhibitor isolated from tamarind seed (TTI) has satietogenic effects in animals, increasing the cholecystokinin (CCK) in eutrophy and reducing leptin in obesity. We purified TTI (pTTI), characterised, and observed its effect upon CCK and leptin in obese Wistar rats. By HPLC, and after amplification of resolution, two protein fractions were observed: Fr1 and Fr2, with average mass of [M þ 14H]þ ¼ 19,594,690 Da and [M þ 13H]þ ¼ 19,578,266 Da, respectively. The protein fractions showed 54 and 53 amino acid residues with the same sequence. pTTI presented resistance to temperature and pH variations; IC50 was 2.7 1010mol.L1 and Ki was 2.9 1011mol.L1 . The 2-DE revealed spots with isoelectric points between pH 5 and 6, and one near pH 8. pTTI action on leptin decrease was confirmed. We conclude that pTTI is a Kunitz trypsin inhibitor with possible biotechnological health-related application
publishDate 2018
dc.date.issued.fl_str_mv 2018-01
dc.date.accessioned.fl_str_mv 2024-02-01T16:00:58Z
dc.date.available.fl_str_mv 2024-02-01T16:00:58Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MEDEIROS, Amanda Fernandes de; COSTA, Izael de Sousa; CARVALHO, Fabiana Maria Coimbra de; KIYOTA, Sumika; SOUZA, Beatriz Blenda Pinheiro de; SIFUENTES, Daniel Nogoceke; SERQUIZ, Raphael Paschoal; MACIEL, Bruna Leal Lima; UCHÔA, Adriana Ferreira; SANTOS, Elizeu Antunes dos; MORAIS, Ana Heloneida de Araújo. Biochemical characterisation of a kunitz-type inhibitor from tamarindus indica L. seeds and its efficacy in reducing plasma leptin in an experimental model of obesity. Journal Of Enzyme Inhibition And Medicinal Chemistry, [S.l.], v. 33, n. 1, p. 334-348, 1 jan. 2018. DOI: 10.1080/14756366.2017.1419220. Disponível em: https://www.tandfonline.com/doi/full/10.1080/14756366.2017.1419220. Acesso em: 30 jan. 2024.
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/handle/123456789/57470
dc.identifier.doi.none.fl_str_mv http://dx.doi.org/10.1080/14756366.2017.1419220
identifier_str_mv MEDEIROS, Amanda Fernandes de; COSTA, Izael de Sousa; CARVALHO, Fabiana Maria Coimbra de; KIYOTA, Sumika; SOUZA, Beatriz Blenda Pinheiro de; SIFUENTES, Daniel Nogoceke; SERQUIZ, Raphael Paschoal; MACIEL, Bruna Leal Lima; UCHÔA, Adriana Ferreira; SANTOS, Elizeu Antunes dos; MORAIS, Ana Heloneida de Araújo. Biochemical characterisation of a kunitz-type inhibitor from tamarindus indica L. seeds and its efficacy in reducing plasma leptin in an experimental model of obesity. Journal Of Enzyme Inhibition And Medicinal Chemistry, [S.l.], v. 33, n. 1, p. 334-348, 1 jan. 2018. DOI: 10.1080/14756366.2017.1419220. Disponível em: https://www.tandfonline.com/doi/full/10.1080/14756366.2017.1419220. Acesso em: 30 jan. 2024.
url https://repositorio.ufrn.br/handle/123456789/57470
http://dx.doi.org/10.1080/14756366.2017.1419220
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution 3.0 Brazil
http://creativecommons.org/licenses/by/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 3.0 Brazil
http://creativecommons.org/licenses/by/3.0/br/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Journal of Enzyme Inhibition and Medicinal Chemistry
publisher.none.fl_str_mv Journal of Enzyme Inhibition and Medicinal Chemistry
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instname:Universidade Federal do Rio Grande do Norte (UFRN)
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