Characterization of RadA/Sms from Chromobacterium violaceum and discovery of a new episome

Detalhes bibliográficos
Autor(a) principal: Lima, Daniel Chaves de
Data de Publicação: 2016
Tipo de documento: Tese
Idioma: por
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/jspui/handle/123456789/22058
Resumo: Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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spelling Lima, Daniel Chaves deTheodoro, Raquel CordeiroSouza, Jorge Estefano Santana deCoutinho, Leonam GomesFarias, Sávio Torres deMedeiros, Silvia Regina Batistuzzo de2017-02-20T18:50:56Z2017-11-14T12:31:55Z2016-09-23LIMA, Daniel Chaves de. Characterization of RadA/Sms from Chromobacterium violaceum and discovery of a new episome. 2016. 105f. Tese (Doutorado em Bioquímica) - Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, 2016.https://repositorio.ufrn.br/jspui/handle/123456789/22058Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Chromobacterium violaceum is a ß-proteobacteria commonly found around tropical and subtropical regions throughout the globe. It produces many metabolites with biotechnological properties such as antitumoral peptides, antibiotics and polymers that have potential to replace the oil-based ones. Although it has been extensively studied over the past 40 years, there are many aspects of C. violaceum that remains unclear until today. We have conducted a biochemical study on the homologous recombination (HR) machinery of C. violaceum, mainly in RecA and its paralog, RadA/Sms. We performed in vitro assays from initial and late steps of HR such as D-loop formation and branch migration, respectively, with their corresponding molecular actors and how RadA/Sms influenced each one. We observed cvRadA/Sms influences negatively D-loop formation promoted by cvRecA and through pull-down assay we have observed an interaction between these two proteins. We also observed the DNA-binding preference of cvRadA/Sms and cvRecA and observed that this protein binds preferentially to dsDNA instead ssDNA, unlike cvRecA. No involvement of cvRadA/Sms on branch migration reactions was detected. In this work, we also described, for the first time, the isolation, sequencing and annotation of a new plasmid from C. violaceum, which we named ChVi1 and has 44,236 base pairs, 39 predicted open reading frames (ORFs) and, possibly, two origins of replication. Most of the ORFs codes for hypothetical and structural bacteriophage proteins. By using restriction digestion and Next-generation sequencing (NGS) we also looked for the presence of a similar plasmid in other seven C. violaceum strains isolated from amazon region. Our analysis suggest the presence of a plasmid similar to ChVi1 in two of these strains. The present work describes for the first time a biochemical characterization of RadA/Sms and RecA from C. violaceum which have different roles in HR. Moreover, the discovery of ChVi1 opens a path to further explore C. violaceum’s biology.porCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICARecombinação homólogaRadA/SmsRecAChromobacterium-violaceumEpisomaCharacterization of RadA/Sms from Chromobacterium violaceum and discovery of a new episomeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisPROGRAMA DE PÓS-GRADUAÇÃO EM BIOQUÍMICAUFRNBrasilinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALCharacterizationRadA/SmsChromobacterium_Lima_2016.pdfCharacterizationRadA/SmsChromobacterium_Lima_2016.pdfapplication/pdf22301653https://repositorio.ufrn.br/bitstream/123456789/22058/1/CharacterizationRadA/SmsChromobacterium_Lima_2016.pdf8d405b2be59f71b1d093873cb2fda162MD51TEXTDanielChavesDeLima_TESE.pdf.txtDanielChavesDeLima_TESE.pdf.txtExtracted texttext/plain190483https://repositorio.ufrn.br/bitstream/123456789/22058/4/DanielChavesDeLima_TESE.pdf.txtec7a37fe202318c865d05e54f22d38abMD54CharacterizationRadA/SmsChromobacterium_Lima_2016.pdf.txtCharacterizationRadA/SmsChromobacterium_Lima_2016.pdf.txtExtracted texttext/plain190483https://repositorio.ufrn.br/bitstream/123456789/22058/6/CharacterizationRadA/SmsChromobacterium_Lima_2016.pdf.txtec7a37fe202318c865d05e54f22d38abMD56THUMBNAILDanielChavesDeLima_TESE.pdf.jpgDanielChavesDeLima_TESE.pdf.jpgIM Thumbnailimage/jpeg4888https://repositorio.ufrn.br/bitstream/123456789/22058/5/DanielChavesDeLima_TESE.pdf.jpg5050ee56761eb62853d3b147fb9ef091MD55CharacterizationRadA/SmsChromobacterium_Lima_2016.pdf.jpgCharacterizationRadA/SmsChromobacterium_Lima_2016.pdf.jpgIM Thumbnailimage/jpeg4892https://repositorio.ufrn.br/bitstream/123456789/22058/7/CharacterizationRadA/SmsChromobacterium_Lima_2016.pdf.jpg37595ef77cc77dbf45ecf9f0f25dbd6bMD57123456789/220582020-02-19 11:24:34.098oai:https://repositorio.ufrn.br:123456789/22058Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2020-02-19T14:24:34Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.pt_BR.fl_str_mv Characterization of RadA/Sms from Chromobacterium violaceum and discovery of a new episome
title Characterization of RadA/Sms from Chromobacterium violaceum and discovery of a new episome
spellingShingle Characterization of RadA/Sms from Chromobacterium violaceum and discovery of a new episome
Lima, Daniel Chaves de
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
Recombinação homóloga
RadA/Sms
RecA
Chromobacterium-violaceum
Episoma
title_short Characterization of RadA/Sms from Chromobacterium violaceum and discovery of a new episome
title_full Characterization of RadA/Sms from Chromobacterium violaceum and discovery of a new episome
title_fullStr Characterization of RadA/Sms from Chromobacterium violaceum and discovery of a new episome
title_full_unstemmed Characterization of RadA/Sms from Chromobacterium violaceum and discovery of a new episome
title_sort Characterization of RadA/Sms from Chromobacterium violaceum and discovery of a new episome
author Lima, Daniel Chaves de
author_facet Lima, Daniel Chaves de
author_role author
dc.contributor.authorID.pt_BR.fl_str_mv
dc.contributor.advisorID.pt_BR.fl_str_mv
dc.contributor.referees1.none.fl_str_mv Theodoro, Raquel Cordeiro
dc.contributor.referees1ID.pt_BR.fl_str_mv
dc.contributor.referees2.none.fl_str_mv Souza, Jorge Estefano Santana de
dc.contributor.referees2ID.pt_BR.fl_str_mv
dc.contributor.referees3.none.fl_str_mv Coutinho, Leonam Gomes
dc.contributor.referees3ID.pt_BR.fl_str_mv
dc.contributor.referees4.none.fl_str_mv Farias, Sávio Torres de
dc.contributor.referees4ID.pt_BR.fl_str_mv
dc.contributor.author.fl_str_mv Lima, Daniel Chaves de
dc.contributor.advisor1.fl_str_mv Medeiros, Silvia Regina Batistuzzo de
contributor_str_mv Medeiros, Silvia Regina Batistuzzo de
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
topic CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
Recombinação homóloga
RadA/Sms
RecA
Chromobacterium-violaceum
Episoma
dc.subject.por.fl_str_mv Recombinação homóloga
RadA/Sms
RecA
Chromobacterium-violaceum
Episoma
description Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
publishDate 2016
dc.date.issued.fl_str_mv 2016-09-23
dc.date.accessioned.fl_str_mv 2017-02-20T18:50:56Z
dc.date.available.fl_str_mv 2017-11-14T12:31:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv LIMA, Daniel Chaves de. Characterization of RadA/Sms from Chromobacterium violaceum and discovery of a new episome. 2016. 105f. Tese (Doutorado em Bioquímica) - Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, 2016.
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/jspui/handle/123456789/22058
identifier_str_mv LIMA, Daniel Chaves de. Characterization of RadA/Sms from Chromobacterium violaceum and discovery of a new episome. 2016. 105f. Tese (Doutorado em Bioquímica) - Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, 2016.
url https://repositorio.ufrn.br/jspui/handle/123456789/22058
dc.language.iso.fl_str_mv por
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.program.fl_str_mv PROGRAMA DE PÓS-GRADUAÇÃO EM BIOQUÍMICA
dc.publisher.initials.fl_str_mv UFRN
dc.publisher.country.fl_str_mv Brasil
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