Production of enzymes by paenibacillus chitinolyticus and paenibacillus ehimensis to obtain chitooligosaccharides

Detalhes bibliográficos
Autor(a) principal: Santos, Everaldo Silvino dos
Data de Publicação: 2013
Outros Autores: Araújo, Nathália Kelly de, Assis, Cristiane Fernandes de, Macedo, Gorete Ribeiro de, Farias, Louise Fonseca de, Arimatéia Júnior, Humberto, Pedrosa, Matheus de Freitas Fernandes, Pagnoncelli, Maria Giovana Binder
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/handle/123456789/32554
Resumo: Obtaining oligosaccharides from chitosan has been the focus of several studies in the pharmaceutical, chemical, food, and medical areas, due to their functional properties. Here, we evaluated the production potential of biologically functional chitooligosaccharides using enzymes extracts produced by Paenibacillus chitinolyticus and Paenibacillus ehimensis. After 48 h of fermentation, these microorganisms were able to produce chitosanases, which generated oligomers with a degree of polymerization between dimers and hexamers. The maximum conversion of chitosan to oligomers was 99.2 %, achieved after 12 h incubation of chitosan with enzymes produced by P. ehimensis. The chitooligosaccharides generated were capable of scavenging the 2,2-diphenyl-1-picrylhydrazyl radical, reaching a maximum scavenging rate of 61 and 39 % when produced with P. ehimensis and P. chitinolyticus enzymes, respectively. The use of these enzymes in the crude form could facilitate their use in industrial applications
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spelling Santos, Everaldo Silvino dosAraújo, Nathália Kelly deAssis, Cristiane Fernandes deMacedo, Gorete Ribeiro deFarias, Louise Fonseca deArimatéia Júnior, HumbertoPedrosa, Matheus de Freitas FernandesPagnoncelli, Maria Giovana Binder2021-05-25T12:36:31Z2021-05-25T12:36:31Z2013-03-17ARAÚJO, Nathália Kelly; ASSIS, Cristiane Fernandes; SANTOS, Everaldo Silvino; MACEDO, Gorete Ribeiro; FARIAS, Louise Fonseca; ARIMATÉIA, Humberto FREITAS, Matheus Fernandes B.; PAGNONCELLI, Maria Giovana Binder . Production of Enzymes by Paenibacillus chitinolyticus and Paenibacillus ehimensis to obtain chitooligosaccharides. Applied Biochemistry and Biotechnology, v. 170, p. 292-300, 2013. Disponível em: https://link.springer.com/article/10.1007%2Fs12010-013-0143-0 Acesso em 08 abr. 2021. https://doi.org/10.1007/s12010-013-0143-00273-22891559-0291https://repositorio.ufrn.br/handle/123456789/3255410.1007/s12010-013-0143-0SpringerAttribution 3.0 Brazilhttp://creativecommons.org/licenses/by/3.0/br/info:eu-repo/semantics/openAccessChitosanPaenibacillus chitinolyticusPaenibacillus ehimensisChitooligosaccharidesAntioxidanProduction of enzymes by paenibacillus chitinolyticus and paenibacillus ehimensis to obtain chitooligosaccharidesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleObtaining oligosaccharides from chitosan has been the focus of several studies in the pharmaceutical, chemical, food, and medical areas, due to their functional properties. Here, we evaluated the production potential of biologically functional chitooligosaccharides using enzymes extracts produced by Paenibacillus chitinolyticus and Paenibacillus ehimensis. After 48 h of fermentation, these microorganisms were able to produce chitosanases, which generated oligomers with a degree of polymerization between dimers and hexamers. The maximum conversion of chitosan to oligomers was 99.2 %, achieved after 12 h incubation of chitosan with enzymes produced by P. ehimensis. The chitooligosaccharides generated were capable of scavenging the 2,2-diphenyl-1-picrylhydrazyl radical, reaching a maximum scavenging rate of 61 and 39 % when produced with P. ehimensis and P. chitinolyticus enzymes, respectively. The use of these enzymes in the crude form could facilitate their use in industrial applicationsengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALProductionOfEnzymesByPaenibaci_Santos_2013.pdfProductionOfEnzymesByPaenibaci_Santos_2013.pdfapplication/pdf192352https://repositorio.ufrn.br/bitstream/123456789/32554/1/ProductionOfEnzymesByPaenibaci_Santos_2013.pdf388bc3b506e6447fac0c81f046f46ecdMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.ufrn.br/bitstream/123456789/32554/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/32554/3/license.txte9597aa2854d128fd968be5edc8a28d9MD53123456789/325542021-05-25 09:36:32.968oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2021-05-25T12:36:32Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.pt_BR.fl_str_mv Production of enzymes by paenibacillus chitinolyticus and paenibacillus ehimensis to obtain chitooligosaccharides
title Production of enzymes by paenibacillus chitinolyticus and paenibacillus ehimensis to obtain chitooligosaccharides
spellingShingle Production of enzymes by paenibacillus chitinolyticus and paenibacillus ehimensis to obtain chitooligosaccharides
Santos, Everaldo Silvino dos
Chitosan
Paenibacillus chitinolyticus
Paenibacillus ehimensis
Chitooligosaccharides
Antioxidan
title_short Production of enzymes by paenibacillus chitinolyticus and paenibacillus ehimensis to obtain chitooligosaccharides
title_full Production of enzymes by paenibacillus chitinolyticus and paenibacillus ehimensis to obtain chitooligosaccharides
title_fullStr Production of enzymes by paenibacillus chitinolyticus and paenibacillus ehimensis to obtain chitooligosaccharides
title_full_unstemmed Production of enzymes by paenibacillus chitinolyticus and paenibacillus ehimensis to obtain chitooligosaccharides
title_sort Production of enzymes by paenibacillus chitinolyticus and paenibacillus ehimensis to obtain chitooligosaccharides
author Santos, Everaldo Silvino dos
author_facet Santos, Everaldo Silvino dos
Araújo, Nathália Kelly de
Assis, Cristiane Fernandes de
Macedo, Gorete Ribeiro de
Farias, Louise Fonseca de
Arimatéia Júnior, Humberto
Pedrosa, Matheus de Freitas Fernandes
Pagnoncelli, Maria Giovana Binder
author_role author
author2 Araújo, Nathália Kelly de
Assis, Cristiane Fernandes de
Macedo, Gorete Ribeiro de
Farias, Louise Fonseca de
Arimatéia Júnior, Humberto
Pedrosa, Matheus de Freitas Fernandes
Pagnoncelli, Maria Giovana Binder
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, Everaldo Silvino dos
Araújo, Nathália Kelly de
Assis, Cristiane Fernandes de
Macedo, Gorete Ribeiro de
Farias, Louise Fonseca de
Arimatéia Júnior, Humberto
Pedrosa, Matheus de Freitas Fernandes
Pagnoncelli, Maria Giovana Binder
dc.subject.por.fl_str_mv Chitosan
Paenibacillus chitinolyticus
Paenibacillus ehimensis
Chitooligosaccharides
Antioxidan
topic Chitosan
Paenibacillus chitinolyticus
Paenibacillus ehimensis
Chitooligosaccharides
Antioxidan
description Obtaining oligosaccharides from chitosan has been the focus of several studies in the pharmaceutical, chemical, food, and medical areas, due to their functional properties. Here, we evaluated the production potential of biologically functional chitooligosaccharides using enzymes extracts produced by Paenibacillus chitinolyticus and Paenibacillus ehimensis. After 48 h of fermentation, these microorganisms were able to produce chitosanases, which generated oligomers with a degree of polymerization between dimers and hexamers. The maximum conversion of chitosan to oligomers was 99.2 %, achieved after 12 h incubation of chitosan with enzymes produced by P. ehimensis. The chitooligosaccharides generated were capable of scavenging the 2,2-diphenyl-1-picrylhydrazyl radical, reaching a maximum scavenging rate of 61 and 39 % when produced with P. ehimensis and P. chitinolyticus enzymes, respectively. The use of these enzymes in the crude form could facilitate their use in industrial applications
publishDate 2013
dc.date.issued.fl_str_mv 2013-03-17
dc.date.accessioned.fl_str_mv 2021-05-25T12:36:31Z
dc.date.available.fl_str_mv 2021-05-25T12:36:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ARAÚJO, Nathália Kelly; ASSIS, Cristiane Fernandes; SANTOS, Everaldo Silvino; MACEDO, Gorete Ribeiro; FARIAS, Louise Fonseca; ARIMATÉIA, Humberto FREITAS, Matheus Fernandes B.; PAGNONCELLI, Maria Giovana Binder . Production of Enzymes by Paenibacillus chitinolyticus and Paenibacillus ehimensis to obtain chitooligosaccharides. Applied Biochemistry and Biotechnology, v. 170, p. 292-300, 2013. Disponível em: https://link.springer.com/article/10.1007%2Fs12010-013-0143-0 Acesso em 08 abr. 2021. https://doi.org/10.1007/s12010-013-0143-0
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/handle/123456789/32554
dc.identifier.issn.none.fl_str_mv 0273-2289
1559-0291
dc.identifier.doi.none.fl_str_mv 10.1007/s12010-013-0143-0
identifier_str_mv ARAÚJO, Nathália Kelly; ASSIS, Cristiane Fernandes; SANTOS, Everaldo Silvino; MACEDO, Gorete Ribeiro; FARIAS, Louise Fonseca; ARIMATÉIA, Humberto FREITAS, Matheus Fernandes B.; PAGNONCELLI, Maria Giovana Binder . Production of Enzymes by Paenibacillus chitinolyticus and Paenibacillus ehimensis to obtain chitooligosaccharides. Applied Biochemistry and Biotechnology, v. 170, p. 292-300, 2013. Disponível em: https://link.springer.com/article/10.1007%2Fs12010-013-0143-0 Acesso em 08 abr. 2021. https://doi.org/10.1007/s12010-013-0143-0
0273-2289
1559-0291
10.1007/s12010-013-0143-0
url https://repositorio.ufrn.br/handle/123456789/32554
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http://creativecommons.org/licenses/by/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 3.0 Brazil
http://creativecommons.org/licenses/by/3.0/br/
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dc.publisher.none.fl_str_mv Springer
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