The let-7b-5p, miR-326, and miR-125a-3p are associated with left ventricular systolic dysfunction in post-myocardial infarction
Autor(a) principal: | |
---|---|
Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/handle/123456789/57727 http://dx.doi.org/10.3389/fcvm.2023.1151855 |
Resumo: | Background: Acute ST-elevation myocardial infarction (STEMI) can lead to adverse cardiac remodeling, resulting in left ventricular systolic dysfunction (LVSd) and heart failure. Epigenetic regulators, such as microRNAs, may be involved in the physiopathology of LVSd. Objective: This study explored microRNAs in peripheral blood mononuclear cells (PBMC) of post-myocardial infarction patients with LVSd. Methods: Post-STEMI patients were grouped as having (LVSd, n = 9) or not LVSd (non-LVSd, n = 16). The expression of 61 microRNAs was analyzed in PBMC by RT-qPCR and the differentially expressed microRNAs were identified. Principal Component Analysis stratified the microRNAs based on the development of dysfunction. Predictive variables of LVSd were investigated through logistic regression analysis. A system biology approach was used to explore the regulatory molecular network of the disease and an enrichment analysis was performed. Results: The let-7b-5p (AUC: 0.807; 95% CI: 0.63–0.98; p = 0.013), miR-125a-3p (AUC: 0.800; 95% CI: 0.61–0.99; p = 0.036) and miR-326 (AUC: 0.783; 95% CI: 0.54–1.00; p = 0.028) were upregulated in LVSd ( p < 0.05) and discriminated LVSd from non-LVSd. Multivariate logistic regression analysis showed let-7b-5p (OR: 16.00; 95% CI: 1.54–166.05; p = 0.020) and miR-326 (OR: 28.00; 95% CI: 2.42–323.70; p = 0.008) as predictors of LVSd. The enrichment analysis revealed association of the targets of these three microRNAs with immunological response, cell-cell adhesion, and cardiac changes. Conclusion: LVSd alters the expression of let-7b-5p, miR-326, and miR-125a-3p in PBMC from post-STEMI, indicating their potential involvement in the cardiac dysfunction physiopathology and highlighting these miRNAs as possible LVSd biomarkers |
id |
UFRN_60fd7958664ecb7aa6511019aa0023e7 |
---|---|
oai_identifier_str |
oai:https://repositorio.ufrn.br:123456789/57727 |
network_acronym_str |
UFRN |
network_name_str |
Repositório Institucional da UFRN |
repository_id_str |
|
spelling |
Reis, Bruna ZavarizeKomatsu, Raquel Costa Silva DantasCruz, Marina SampaioFreire, Paula PaccielliDiniz, Rosiane Viana ZuzaBortolin, Raul HernandesMarques, Otávio CabralSouza, Kamilla Batista da SilvaHirata, Mario HiroyukiHirata, Rosario Dominguez CrespoJurisica, IgorSilbiger, Vivian NogueiraLuchessi, André Ducati2024-03-01T21:21:09Z2024-03-01T21:21:09Z2023-05KOMATSU, Raquel Costa Silva Dantas; CRUZ, Marina Sampaio; FREIRE, Paula Paccielli; DINIZ, Rosiane Viana Zuza; BORTOLIN, Raul Hernandes; MARQUES, Otávio Cabral; SOUZA, Kamilla Batista da Silva; HIRATA, Mario Hiroyuki; HIRATA, Rosario Dominguez Crespo; REIS, Bruna Zavarize; JURISICA, Igor; SILBIGER, Vivian Nogueira; LUCHESSI, André Ducati. The let-7b-5p, miR-326, and miR-125a-3p are associated with left ventricular systolic dysfunction in post-myocardial infarction. Frontiers in Cardiovascular Medicine, [S.l.], v. 10, p. 1-11, 12 mai. 2023. DOI: 10.3389/fcvm.2023.1151855. Disponível em: https://www.frontiersin.org/articles/10.3389/fcvm.2023.1151855/full. Acesso em: 16 fev. 2024.https://repositorio.ufrn.br/handle/123456789/57727http://dx.doi.org/10.3389/fcvm.2023.1151855Frontiers in Cardiovascular MedicineAttribution 3.0 Brazilhttp://creativecommons.org/licenses/by/3.0/br/info:eu-repo/semantics/openAccessmiRNAPBMCMyocardial infarctionLeft ventricular systolic dysfunctionHeart failureEnrichment analysisThe let-7b-5p, miR-326, and miR-125a-3p are associated with left ventricular systolic dysfunction in post-myocardial infarctioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBackground: Acute ST-elevation myocardial infarction (STEMI) can lead to adverse cardiac remodeling, resulting in left ventricular systolic dysfunction (LVSd) and heart failure. Epigenetic regulators, such as microRNAs, may be involved in the physiopathology of LVSd. Objective: This study explored microRNAs in peripheral blood mononuclear cells (PBMC) of post-myocardial infarction patients with LVSd. Methods: Post-STEMI patients were grouped as having (LVSd, n = 9) or not LVSd (non-LVSd, n = 16). The expression of 61 microRNAs was analyzed in PBMC by RT-qPCR and the differentially expressed microRNAs were identified. Principal Component Analysis stratified the microRNAs based on the development of dysfunction. Predictive variables of LVSd were investigated through logistic regression analysis. A system biology approach was used to explore the regulatory molecular network of the disease and an enrichment analysis was performed. Results: The let-7b-5p (AUC: 0.807; 95% CI: 0.63–0.98; p = 0.013), miR-125a-3p (AUC: 0.800; 95% CI: 0.61–0.99; p = 0.036) and miR-326 (AUC: 0.783; 95% CI: 0.54–1.00; p = 0.028) were upregulated in LVSd ( p < 0.05) and discriminated LVSd from non-LVSd. Multivariate logistic regression analysis showed let-7b-5p (OR: 16.00; 95% CI: 1.54–166.05; p = 0.020) and miR-326 (OR: 28.00; 95% CI: 2.42–323.70; p = 0.008) as predictors of LVSd. The enrichment analysis revealed association of the targets of these three microRNAs with immunological response, cell-cell adhesion, and cardiac changes. Conclusion: LVSd alters the expression of let-7b-5p, miR-326, and miR-125a-3p in PBMC from post-STEMI, indicating their potential involvement in the cardiac dysfunction physiopathology and highlighting these miRNAs as possible LVSd biomarkersengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALLet-7B-5P_Komatsu_2023.pdfLet-7B-5P_Komatsu_2023.pdfapplication/pdf35598860https://repositorio.ufrn.br/bitstream/123456789/57727/1/Let-7B-5P_Komatsu_2023.pdf398495de5d10404c9b436aea924f097bMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.ufrn.br/bitstream/123456789/57727/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/57727/3/license.txte9597aa2854d128fd968be5edc8a28d9MD53123456789/577272024-03-01 18:21:10.764oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2024-03-01T21:21:10Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
The let-7b-5p, miR-326, and miR-125a-3p are associated with left ventricular systolic dysfunction in post-myocardial infarction |
title |
The let-7b-5p, miR-326, and miR-125a-3p are associated with left ventricular systolic dysfunction in post-myocardial infarction |
spellingShingle |
The let-7b-5p, miR-326, and miR-125a-3p are associated with left ventricular systolic dysfunction in post-myocardial infarction Reis, Bruna Zavarize miRNA PBMC Myocardial infarction Left ventricular systolic dysfunction Heart failure Enrichment analysis |
title_short |
The let-7b-5p, miR-326, and miR-125a-3p are associated with left ventricular systolic dysfunction in post-myocardial infarction |
title_full |
The let-7b-5p, miR-326, and miR-125a-3p are associated with left ventricular systolic dysfunction in post-myocardial infarction |
title_fullStr |
The let-7b-5p, miR-326, and miR-125a-3p are associated with left ventricular systolic dysfunction in post-myocardial infarction |
title_full_unstemmed |
The let-7b-5p, miR-326, and miR-125a-3p are associated with left ventricular systolic dysfunction in post-myocardial infarction |
title_sort |
The let-7b-5p, miR-326, and miR-125a-3p are associated with left ventricular systolic dysfunction in post-myocardial infarction |
author |
Reis, Bruna Zavarize |
author_facet |
Reis, Bruna Zavarize Komatsu, Raquel Costa Silva Dantas Cruz, Marina Sampaio Freire, Paula Paccielli Diniz, Rosiane Viana Zuza Bortolin, Raul Hernandes Marques, Otávio Cabral Souza, Kamilla Batista da Silva Hirata, Mario Hiroyuki Hirata, Rosario Dominguez Crespo Jurisica, Igor Silbiger, Vivian Nogueira Luchessi, André Ducati |
author_role |
author |
author2 |
Komatsu, Raquel Costa Silva Dantas Cruz, Marina Sampaio Freire, Paula Paccielli Diniz, Rosiane Viana Zuza Bortolin, Raul Hernandes Marques, Otávio Cabral Souza, Kamilla Batista da Silva Hirata, Mario Hiroyuki Hirata, Rosario Dominguez Crespo Jurisica, Igor Silbiger, Vivian Nogueira Luchessi, André Ducati |
author2_role |
author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Reis, Bruna Zavarize Komatsu, Raquel Costa Silva Dantas Cruz, Marina Sampaio Freire, Paula Paccielli Diniz, Rosiane Viana Zuza Bortolin, Raul Hernandes Marques, Otávio Cabral Souza, Kamilla Batista da Silva Hirata, Mario Hiroyuki Hirata, Rosario Dominguez Crespo Jurisica, Igor Silbiger, Vivian Nogueira Luchessi, André Ducati |
dc.subject.por.fl_str_mv |
miRNA PBMC Myocardial infarction Left ventricular systolic dysfunction Heart failure Enrichment analysis |
topic |
miRNA PBMC Myocardial infarction Left ventricular systolic dysfunction Heart failure Enrichment analysis |
description |
Background: Acute ST-elevation myocardial infarction (STEMI) can lead to adverse cardiac remodeling, resulting in left ventricular systolic dysfunction (LVSd) and heart failure. Epigenetic regulators, such as microRNAs, may be involved in the physiopathology of LVSd. Objective: This study explored microRNAs in peripheral blood mononuclear cells (PBMC) of post-myocardial infarction patients with LVSd. Methods: Post-STEMI patients were grouped as having (LVSd, n = 9) or not LVSd (non-LVSd, n = 16). The expression of 61 microRNAs was analyzed in PBMC by RT-qPCR and the differentially expressed microRNAs were identified. Principal Component Analysis stratified the microRNAs based on the development of dysfunction. Predictive variables of LVSd were investigated through logistic regression analysis. A system biology approach was used to explore the regulatory molecular network of the disease and an enrichment analysis was performed. Results: The let-7b-5p (AUC: 0.807; 95% CI: 0.63–0.98; p = 0.013), miR-125a-3p (AUC: 0.800; 95% CI: 0.61–0.99; p = 0.036) and miR-326 (AUC: 0.783; 95% CI: 0.54–1.00; p = 0.028) were upregulated in LVSd ( p < 0.05) and discriminated LVSd from non-LVSd. Multivariate logistic regression analysis showed let-7b-5p (OR: 16.00; 95% CI: 1.54–166.05; p = 0.020) and miR-326 (OR: 28.00; 95% CI: 2.42–323.70; p = 0.008) as predictors of LVSd. The enrichment analysis revealed association of the targets of these three microRNAs with immunological response, cell-cell adhesion, and cardiac changes. Conclusion: LVSd alters the expression of let-7b-5p, miR-326, and miR-125a-3p in PBMC from post-STEMI, indicating their potential involvement in the cardiac dysfunction physiopathology and highlighting these miRNAs as possible LVSd biomarkers |
publishDate |
2023 |
dc.date.issued.fl_str_mv |
2023-05 |
dc.date.accessioned.fl_str_mv |
2024-03-01T21:21:09Z |
dc.date.available.fl_str_mv |
2024-03-01T21:21:09Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
KOMATSU, Raquel Costa Silva Dantas; CRUZ, Marina Sampaio; FREIRE, Paula Paccielli; DINIZ, Rosiane Viana Zuza; BORTOLIN, Raul Hernandes; MARQUES, Otávio Cabral; SOUZA, Kamilla Batista da Silva; HIRATA, Mario Hiroyuki; HIRATA, Rosario Dominguez Crespo; REIS, Bruna Zavarize; JURISICA, Igor; SILBIGER, Vivian Nogueira; LUCHESSI, André Ducati. The let-7b-5p, miR-326, and miR-125a-3p are associated with left ventricular systolic dysfunction in post-myocardial infarction. Frontiers in Cardiovascular Medicine, [S.l.], v. 10, p. 1-11, 12 mai. 2023. DOI: 10.3389/fcvm.2023.1151855. Disponível em: https://www.frontiersin.org/articles/10.3389/fcvm.2023.1151855/full. Acesso em: 16 fev. 2024. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/handle/123456789/57727 |
dc.identifier.doi.none.fl_str_mv |
http://dx.doi.org/10.3389/fcvm.2023.1151855 |
identifier_str_mv |
KOMATSU, Raquel Costa Silva Dantas; CRUZ, Marina Sampaio; FREIRE, Paula Paccielli; DINIZ, Rosiane Viana Zuza; BORTOLIN, Raul Hernandes; MARQUES, Otávio Cabral; SOUZA, Kamilla Batista da Silva; HIRATA, Mario Hiroyuki; HIRATA, Rosario Dominguez Crespo; REIS, Bruna Zavarize; JURISICA, Igor; SILBIGER, Vivian Nogueira; LUCHESSI, André Ducati. The let-7b-5p, miR-326, and miR-125a-3p are associated with left ventricular systolic dysfunction in post-myocardial infarction. Frontiers in Cardiovascular Medicine, [S.l.], v. 10, p. 1-11, 12 mai. 2023. DOI: 10.3389/fcvm.2023.1151855. Disponível em: https://www.frontiersin.org/articles/10.3389/fcvm.2023.1151855/full. Acesso em: 16 fev. 2024. |
url |
https://repositorio.ufrn.br/handle/123456789/57727 http://dx.doi.org/10.3389/fcvm.2023.1151855 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
Attribution 3.0 Brazil http://creativecommons.org/licenses/by/3.0/br/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution 3.0 Brazil http://creativecommons.org/licenses/by/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Frontiers in Cardiovascular Medicine |
publisher.none.fl_str_mv |
Frontiers in Cardiovascular Medicine |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFRN instname:Universidade Federal do Rio Grande do Norte (UFRN) instacron:UFRN |
instname_str |
Universidade Federal do Rio Grande do Norte (UFRN) |
instacron_str |
UFRN |
institution |
UFRN |
reponame_str |
Repositório Institucional da UFRN |
collection |
Repositório Institucional da UFRN |
bitstream.url.fl_str_mv |
https://repositorio.ufrn.br/bitstream/123456789/57727/1/Let-7B-5P_Komatsu_2023.pdf https://repositorio.ufrn.br/bitstream/123456789/57727/2/license_rdf https://repositorio.ufrn.br/bitstream/123456789/57727/3/license.txt |
bitstream.checksum.fl_str_mv |
398495de5d10404c9b436aea924f097b 4d2950bda3d176f570a9f8b328dfbbef e9597aa2854d128fd968be5edc8a28d9 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN) |
repository.mail.fl_str_mv |
|
_version_ |
1814832964274487296 |