The let-7b-5p, miR-326, and miR-125a-3p are associated with left ventricular systolic dysfunction in post-myocardial infarction

Detalhes bibliográficos
Autor(a) principal: Reis, Bruna Zavarize
Data de Publicação: 2023
Outros Autores: Komatsu, Raquel Costa Silva Dantas, Cruz, Marina Sampaio, Freire, Paula Paccielli, Diniz, Rosiane Viana Zuza, Bortolin, Raul Hernandes, Marques, Otávio Cabral, Souza, Kamilla Batista da Silva, Hirata, Mario Hiroyuki, Hirata, Rosario Dominguez Crespo, Jurisica, Igor, Silbiger, Vivian Nogueira, Luchessi, André Ducati
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/handle/123456789/57727
http://dx.doi.org/10.3389/fcvm.2023.1151855
Resumo: Background: Acute ST-elevation myocardial infarction (STEMI) can lead to adverse cardiac remodeling, resulting in left ventricular systolic dysfunction (LVSd) and heart failure. Epigenetic regulators, such as microRNAs, may be involved in the physiopathology of LVSd. Objective: This study explored microRNAs in peripheral blood mononuclear cells (PBMC) of post-myocardial infarction patients with LVSd. Methods: Post-STEMI patients were grouped as having (LVSd, n = 9) or not LVSd (non-LVSd, n = 16). The expression of 61 microRNAs was analyzed in PBMC by RT-qPCR and the differentially expressed microRNAs were identified. Principal Component Analysis stratified the microRNAs based on the development of dysfunction. Predictive variables of LVSd were investigated through logistic regression analysis. A system biology approach was used to explore the regulatory molecular network of the disease and an enrichment analysis was performed. Results: The let-7b-5p (AUC: 0.807; 95% CI: 0.63–0.98; p = 0.013), miR-125a-3p (AUC: 0.800; 95% CI: 0.61–0.99; p = 0.036) and miR-326 (AUC: 0.783; 95% CI: 0.54–1.00; p = 0.028) were upregulated in LVSd ( p < 0.05) and discriminated LVSd from non-LVSd. Multivariate logistic regression analysis showed let-7b-5p (OR: 16.00; 95% CI: 1.54–166.05; p = 0.020) and miR-326 (OR: 28.00; 95% CI: 2.42–323.70; p = 0.008) as predictors of LVSd. The enrichment analysis revealed association of the targets of these three microRNAs with immunological response, cell-cell adhesion, and cardiac changes. Conclusion: LVSd alters the expression of let-7b-5p, miR-326, and miR-125a-3p in PBMC from post-STEMI, indicating their potential involvement in the cardiac dysfunction physiopathology and highlighting these miRNAs as possible LVSd biomarkers
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spelling Reis, Bruna ZavarizeKomatsu, Raquel Costa Silva DantasCruz, Marina SampaioFreire, Paula PaccielliDiniz, Rosiane Viana ZuzaBortolin, Raul HernandesMarques, Otávio CabralSouza, Kamilla Batista da SilvaHirata, Mario HiroyukiHirata, Rosario Dominguez CrespoJurisica, IgorSilbiger, Vivian NogueiraLuchessi, André Ducati2024-03-01T21:21:09Z2024-03-01T21:21:09Z2023-05KOMATSU, Raquel Costa Silva Dantas; CRUZ, Marina Sampaio; FREIRE, Paula Paccielli; DINIZ, Rosiane Viana Zuza; BORTOLIN, Raul Hernandes; MARQUES, Otávio Cabral; SOUZA, Kamilla Batista da Silva; HIRATA, Mario Hiroyuki; HIRATA, Rosario Dominguez Crespo; REIS, Bruna Zavarize; JURISICA, Igor; SILBIGER, Vivian Nogueira; LUCHESSI, André Ducati. The let-7b-5p, miR-326, and miR-125a-3p are associated with left ventricular systolic dysfunction in post-myocardial infarction. Frontiers in Cardiovascular Medicine, [S.l.], v. 10, p. 1-11, 12 mai. 2023. DOI: 10.3389/fcvm.2023.1151855. Disponível em: https://www.frontiersin.org/articles/10.3389/fcvm.2023.1151855/full. Acesso em: 16 fev. 2024.https://repositorio.ufrn.br/handle/123456789/57727http://dx.doi.org/10.3389/fcvm.2023.1151855Frontiers in Cardiovascular MedicineAttribution 3.0 Brazilhttp://creativecommons.org/licenses/by/3.0/br/info:eu-repo/semantics/openAccessmiRNAPBMCMyocardial infarctionLeft ventricular systolic dysfunctionHeart failureEnrichment analysisThe let-7b-5p, miR-326, and miR-125a-3p are associated with left ventricular systolic dysfunction in post-myocardial infarctioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBackground: Acute ST-elevation myocardial infarction (STEMI) can lead to adverse cardiac remodeling, resulting in left ventricular systolic dysfunction (LVSd) and heart failure. Epigenetic regulators, such as microRNAs, may be involved in the physiopathology of LVSd. Objective: This study explored microRNAs in peripheral blood mononuclear cells (PBMC) of post-myocardial infarction patients with LVSd. Methods: Post-STEMI patients were grouped as having (LVSd, n = 9) or not LVSd (non-LVSd, n = 16). The expression of 61 microRNAs was analyzed in PBMC by RT-qPCR and the differentially expressed microRNAs were identified. Principal Component Analysis stratified the microRNAs based on the development of dysfunction. Predictive variables of LVSd were investigated through logistic regression analysis. A system biology approach was used to explore the regulatory molecular network of the disease and an enrichment analysis was performed. Results: The let-7b-5p (AUC: 0.807; 95% CI: 0.63–0.98; p = 0.013), miR-125a-3p (AUC: 0.800; 95% CI: 0.61–0.99; p = 0.036) and miR-326 (AUC: 0.783; 95% CI: 0.54–1.00; p = 0.028) were upregulated in LVSd ( p < 0.05) and discriminated LVSd from non-LVSd. Multivariate logistic regression analysis showed let-7b-5p (OR: 16.00; 95% CI: 1.54–166.05; p = 0.020) and miR-326 (OR: 28.00; 95% CI: 2.42–323.70; p = 0.008) as predictors of LVSd. The enrichment analysis revealed association of the targets of these three microRNAs with immunological response, cell-cell adhesion, and cardiac changes. Conclusion: LVSd alters the expression of let-7b-5p, miR-326, and miR-125a-3p in PBMC from post-STEMI, indicating their potential involvement in the cardiac dysfunction physiopathology and highlighting these miRNAs as possible LVSd biomarkersengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALLet-7B-5P_Komatsu_2023.pdfLet-7B-5P_Komatsu_2023.pdfapplication/pdf35598860https://repositorio.ufrn.br/bitstream/123456789/57727/1/Let-7B-5P_Komatsu_2023.pdf398495de5d10404c9b436aea924f097bMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.ufrn.br/bitstream/123456789/57727/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/57727/3/license.txte9597aa2854d128fd968be5edc8a28d9MD53123456789/577272024-03-01 18:21:10.764oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2024-03-01T21:21:10Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.pt_BR.fl_str_mv The let-7b-5p, miR-326, and miR-125a-3p are associated with left ventricular systolic dysfunction in post-myocardial infarction
title The let-7b-5p, miR-326, and miR-125a-3p are associated with left ventricular systolic dysfunction in post-myocardial infarction
spellingShingle The let-7b-5p, miR-326, and miR-125a-3p are associated with left ventricular systolic dysfunction in post-myocardial infarction
Reis, Bruna Zavarize
miRNA
PBMC
Myocardial infarction
Left ventricular systolic dysfunction
Heart failure
Enrichment analysis
title_short The let-7b-5p, miR-326, and miR-125a-3p are associated with left ventricular systolic dysfunction in post-myocardial infarction
title_full The let-7b-5p, miR-326, and miR-125a-3p are associated with left ventricular systolic dysfunction in post-myocardial infarction
title_fullStr The let-7b-5p, miR-326, and miR-125a-3p are associated with left ventricular systolic dysfunction in post-myocardial infarction
title_full_unstemmed The let-7b-5p, miR-326, and miR-125a-3p are associated with left ventricular systolic dysfunction in post-myocardial infarction
title_sort The let-7b-5p, miR-326, and miR-125a-3p are associated with left ventricular systolic dysfunction in post-myocardial infarction
author Reis, Bruna Zavarize
author_facet Reis, Bruna Zavarize
Komatsu, Raquel Costa Silva Dantas
Cruz, Marina Sampaio
Freire, Paula Paccielli
Diniz, Rosiane Viana Zuza
Bortolin, Raul Hernandes
Marques, Otávio Cabral
Souza, Kamilla Batista da Silva
Hirata, Mario Hiroyuki
Hirata, Rosario Dominguez Crespo
Jurisica, Igor
Silbiger, Vivian Nogueira
Luchessi, André Ducati
author_role author
author2 Komatsu, Raquel Costa Silva Dantas
Cruz, Marina Sampaio
Freire, Paula Paccielli
Diniz, Rosiane Viana Zuza
Bortolin, Raul Hernandes
Marques, Otávio Cabral
Souza, Kamilla Batista da Silva
Hirata, Mario Hiroyuki
Hirata, Rosario Dominguez Crespo
Jurisica, Igor
Silbiger, Vivian Nogueira
Luchessi, André Ducati
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Reis, Bruna Zavarize
Komatsu, Raquel Costa Silva Dantas
Cruz, Marina Sampaio
Freire, Paula Paccielli
Diniz, Rosiane Viana Zuza
Bortolin, Raul Hernandes
Marques, Otávio Cabral
Souza, Kamilla Batista da Silva
Hirata, Mario Hiroyuki
Hirata, Rosario Dominguez Crespo
Jurisica, Igor
Silbiger, Vivian Nogueira
Luchessi, André Ducati
dc.subject.por.fl_str_mv miRNA
PBMC
Myocardial infarction
Left ventricular systolic dysfunction
Heart failure
Enrichment analysis
topic miRNA
PBMC
Myocardial infarction
Left ventricular systolic dysfunction
Heart failure
Enrichment analysis
description Background: Acute ST-elevation myocardial infarction (STEMI) can lead to adverse cardiac remodeling, resulting in left ventricular systolic dysfunction (LVSd) and heart failure. Epigenetic regulators, such as microRNAs, may be involved in the physiopathology of LVSd. Objective: This study explored microRNAs in peripheral blood mononuclear cells (PBMC) of post-myocardial infarction patients with LVSd. Methods: Post-STEMI patients were grouped as having (LVSd, n = 9) or not LVSd (non-LVSd, n = 16). The expression of 61 microRNAs was analyzed in PBMC by RT-qPCR and the differentially expressed microRNAs were identified. Principal Component Analysis stratified the microRNAs based on the development of dysfunction. Predictive variables of LVSd were investigated through logistic regression analysis. A system biology approach was used to explore the regulatory molecular network of the disease and an enrichment analysis was performed. Results: The let-7b-5p (AUC: 0.807; 95% CI: 0.63–0.98; p = 0.013), miR-125a-3p (AUC: 0.800; 95% CI: 0.61–0.99; p = 0.036) and miR-326 (AUC: 0.783; 95% CI: 0.54–1.00; p = 0.028) were upregulated in LVSd ( p < 0.05) and discriminated LVSd from non-LVSd. Multivariate logistic regression analysis showed let-7b-5p (OR: 16.00; 95% CI: 1.54–166.05; p = 0.020) and miR-326 (OR: 28.00; 95% CI: 2.42–323.70; p = 0.008) as predictors of LVSd. The enrichment analysis revealed association of the targets of these three microRNAs with immunological response, cell-cell adhesion, and cardiac changes. Conclusion: LVSd alters the expression of let-7b-5p, miR-326, and miR-125a-3p in PBMC from post-STEMI, indicating their potential involvement in the cardiac dysfunction physiopathology and highlighting these miRNAs as possible LVSd biomarkers
publishDate 2023
dc.date.issued.fl_str_mv 2023-05
dc.date.accessioned.fl_str_mv 2024-03-01T21:21:09Z
dc.date.available.fl_str_mv 2024-03-01T21:21:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv KOMATSU, Raquel Costa Silva Dantas; CRUZ, Marina Sampaio; FREIRE, Paula Paccielli; DINIZ, Rosiane Viana Zuza; BORTOLIN, Raul Hernandes; MARQUES, Otávio Cabral; SOUZA, Kamilla Batista da Silva; HIRATA, Mario Hiroyuki; HIRATA, Rosario Dominguez Crespo; REIS, Bruna Zavarize; JURISICA, Igor; SILBIGER, Vivian Nogueira; LUCHESSI, André Ducati. The let-7b-5p, miR-326, and miR-125a-3p are associated with left ventricular systolic dysfunction in post-myocardial infarction. Frontiers in Cardiovascular Medicine, [S.l.], v. 10, p. 1-11, 12 mai. 2023. DOI: 10.3389/fcvm.2023.1151855. Disponível em: https://www.frontiersin.org/articles/10.3389/fcvm.2023.1151855/full. Acesso em: 16 fev. 2024.
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/handle/123456789/57727
dc.identifier.doi.none.fl_str_mv http://dx.doi.org/10.3389/fcvm.2023.1151855
identifier_str_mv KOMATSU, Raquel Costa Silva Dantas; CRUZ, Marina Sampaio; FREIRE, Paula Paccielli; DINIZ, Rosiane Viana Zuza; BORTOLIN, Raul Hernandes; MARQUES, Otávio Cabral; SOUZA, Kamilla Batista da Silva; HIRATA, Mario Hiroyuki; HIRATA, Rosario Dominguez Crespo; REIS, Bruna Zavarize; JURISICA, Igor; SILBIGER, Vivian Nogueira; LUCHESSI, André Ducati. The let-7b-5p, miR-326, and miR-125a-3p are associated with left ventricular systolic dysfunction in post-myocardial infarction. Frontiers in Cardiovascular Medicine, [S.l.], v. 10, p. 1-11, 12 mai. 2023. DOI: 10.3389/fcvm.2023.1151855. Disponível em: https://www.frontiersin.org/articles/10.3389/fcvm.2023.1151855/full. Acesso em: 16 fev. 2024.
url https://repositorio.ufrn.br/handle/123456789/57727
http://dx.doi.org/10.3389/fcvm.2023.1151855
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution 3.0 Brazil
http://creativecommons.org/licenses/by/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 3.0 Brazil
http://creativecommons.org/licenses/by/3.0/br/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers in Cardiovascular Medicine
publisher.none.fl_str_mv Frontiers in Cardiovascular Medicine
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFRN
instname:Universidade Federal do Rio Grande do Norte (UFRN)
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reponame_str Repositório Institucional da UFRN
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