Influence of laparoscopy and laparotomy on gasometry, leukocytes and cytokines in a rat abdominal sepsis model
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/jspui/handle/1/6299 |
Resumo: | Laparoscopic surgery is associated with reduced surgical trauma, and less acute phase response, as compared with open surgery. Cytokines are important regulators of the biological response to surgical and anesthetic stress. The aim of this study was to determine if CO2 pneumoperitoneum would change cytokine expression, gas parameters and leukocyte count in septic rats. Methods: Wistar rats were randomly assigned to five groups: control (anesthesia only), laparotomy, CO2 pneumoperitoneum, cecum ligation and puncture by laparotomy, and laparoscopic cecum ligation and puncture. After 30 min of the procedures, arterial blood samples were obtained to determine leukocytes subpopulations by hemocytometer. TNFα, IL-1β, IL-6 were determined in intraperitoneal fluid (by ELISA). Gas parameters were measured on arterial blood, intraperitoneal and subperitoneal exsudates. Results: Peritoneal TNFα, IL-1β and IL-6 concentrations were lower in pneumoperitoneum rats than in all other groups (p<0.05). TNFα, IL-1β and IL-6 expression was lower in the laparoscopic than in laparotomic sepsis (p<0.05). Rats from laparoscopic cecum ligation and puncture group developed significant hypercarbic acidosis in blood and subperitoneal fluid when compared to open procedure group. Total white blood cells and lymphocytes were significantly lower in laparoscopic cecum ligation and puncture rats than in the laparotomic (p<0.01). Nevertheless, the laparotomic cecum ligation rats had a significant increase in blood neutrophils and eosinophils when compared with controls (p<0.05). Conclusions: This study demonstrates that the CO2 pneumoperitoneum reduced the inflammatory response in an animal model of peritonitis with respect to intraperitoneal cytokines, white blood cell count and clinical correlates of sepsis. The pneumoperitoneum produced hypercarbic acidosis in septic animals |
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Araújo Filho, IramiHonorato Sobrinho, Abraão AllenRego, Amália Cinthia Meneses doGarcia, Ana Claudia M. de AmorimFernandes, Daniele PimentelCruz, Thaís MedeirosCosta, Ticiana Cabral daMedeiros, Aldo Cunha2013-09-30T16:23:59Z2013-09-30T16:23:59Z2006ARAÚJO FILHO, Irami ; HONORATO SOBRINHO, Abraão Allen ; REGO, Amália Cinthia Meneses do ; GARCIA, Ana Claudia M. de Amorim ; FERNANDES, Daniele Pimentel ; CRUZ, Thaís Medeiros ; COSTA, Ticiana Cabral da ; MEDEIROS, Aldo Cunha. Influence of laparoscopy and laparotomy on gasometry, leukocytes and cytokines in a rat abdominal sepsis model. Acta Cirúrgica Brasileira , Brasil, v. 21, n.2, p. 74-79, 2006. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502006000200004> Acesso em: 20 set. 2013.1678-2674https://repositorio.ufrn.br/jspui/handle/1/6299Laparoscopic surgery is associated with reduced surgical trauma, and less acute phase response, as compared with open surgery. Cytokines are important regulators of the biological response to surgical and anesthetic stress. The aim of this study was to determine if CO2 pneumoperitoneum would change cytokine expression, gas parameters and leukocyte count in septic rats. Methods: Wistar rats were randomly assigned to five groups: control (anesthesia only), laparotomy, CO2 pneumoperitoneum, cecum ligation and puncture by laparotomy, and laparoscopic cecum ligation and puncture. After 30 min of the procedures, arterial blood samples were obtained to determine leukocytes subpopulations by hemocytometer. TNFα, IL-1β, IL-6 were determined in intraperitoneal fluid (by ELISA). Gas parameters were measured on arterial blood, intraperitoneal and subperitoneal exsudates. Results: Peritoneal TNFα, IL-1β and IL-6 concentrations were lower in pneumoperitoneum rats than in all other groups (p<0.05). TNFα, IL-1β and IL-6 expression was lower in the laparoscopic than in laparotomic sepsis (p<0.05). Rats from laparoscopic cecum ligation and puncture group developed significant hypercarbic acidosis in blood and subperitoneal fluid when compared to open procedure group. Total white blood cells and lymphocytes were significantly lower in laparoscopic cecum ligation and puncture rats than in the laparotomic (p<0.01). Nevertheless, the laparotomic cecum ligation rats had a significant increase in blood neutrophils and eosinophils when compared with controls (p<0.05). Conclusions: This study demonstrates that the CO2 pneumoperitoneum reduced the inflammatory response in an animal model of peritonitis with respect to intraperitoneal cytokines, white blood cell count and clinical correlates of sepsis. The pneumoperitoneum produced hypercarbic acidosis in septic animalsengPneumoperitoneumCarbon DioxideSepsisAcidosisLeucocytesInfluence of laparoscopy and laparotomy on gasometry, leukocytes and cytokines in a rat abdominal sepsis modelInfluência da laparoscopia e laparotomia na gasometria, leucócitos e citocinas em modelo de sepse abdominal em ratosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALInfluenceLaparoscopyLaparotomy_Araujo_2006.pdfInfluenceLaparoscopyLaparotomy_Araujo_2006.pdfapplication/pdf124953https://repositorio.ufrn.br/bitstream/1/6299/1/InfluenceLaparoscopyLaparotomy_Araujo_2006.pdfeecd400c52e276d78a75a799e6f7b18aMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-823898https://repositorio.ufrn.br/bitstream/1/6299/2/license_rdfe363e809996cf46ada20da1accfcd9c7MD52license_textlicense_texttext/html; charset=utf-80https://repositorio.ufrn.br/bitstream/1/6299/3/license_textd41d8cd98f00b204e9800998ecf8427eMD53license_urllicense_urltext/plain; charset=utf-852https://repositorio.ufrn.br/bitstream/1/6299/4/license_url3d480ae6c91e310daba2020f8787d6f9MD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.ufrn.br/bitstream/1/6299/5/license.txt8a4605be74aa9ea9d79846c1fba20a33MD55TEXTAldoCM_Influence of laparoscopy_28469.pdf.txtAldoCM_Influence of laparoscopy_28469.pdf.txtExtracted texttext/plain33861https://repositorio.ufrn.br/bitstream/1/6299/10/AldoCM_Influence%20of%20laparoscopy_28469.pdf.txt892da70d4a34d2a3b7a9ec79222b409dMD510THUMBNAILAldoCM_Influence of laparoscopy_28469.pdf.jpgAldoCM_Influence of laparoscopy_28469.pdf.jpgIM Thumbnailimage/jpeg8134https://repositorio.ufrn.br/bitstream/1/6299/11/AldoCM_Influence%20of%20laparoscopy_28469.pdf.jpg0442bd6b786cba24d82e6cf37089ce64MD5111/62992021-11-30 17:13:02.466oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2021-11-30T20:13:02Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
Influence of laparoscopy and laparotomy on gasometry, leukocytes and cytokines in a rat abdominal sepsis model |
dc.title.alternative.pt_BR.fl_str_mv |
Influência da laparoscopia e laparotomia na gasometria, leucócitos e citocinas em modelo de sepse abdominal em ratos |
title |
Influence of laparoscopy and laparotomy on gasometry, leukocytes and cytokines in a rat abdominal sepsis model |
spellingShingle |
Influence of laparoscopy and laparotomy on gasometry, leukocytes and cytokines in a rat abdominal sepsis model Araújo Filho, Irami Pneumoperitoneum Carbon Dioxide Sepsis Acidosis Leucocytes |
title_short |
Influence of laparoscopy and laparotomy on gasometry, leukocytes and cytokines in a rat abdominal sepsis model |
title_full |
Influence of laparoscopy and laparotomy on gasometry, leukocytes and cytokines in a rat abdominal sepsis model |
title_fullStr |
Influence of laparoscopy and laparotomy on gasometry, leukocytes and cytokines in a rat abdominal sepsis model |
title_full_unstemmed |
Influence of laparoscopy and laparotomy on gasometry, leukocytes and cytokines in a rat abdominal sepsis model |
title_sort |
Influence of laparoscopy and laparotomy on gasometry, leukocytes and cytokines in a rat abdominal sepsis model |
author |
Araújo Filho, Irami |
author_facet |
Araújo Filho, Irami Honorato Sobrinho, Abraão Allen Rego, Amália Cinthia Meneses do Garcia, Ana Claudia M. de Amorim Fernandes, Daniele Pimentel Cruz, Thaís Medeiros Costa, Ticiana Cabral da Medeiros, Aldo Cunha |
author_role |
author |
author2 |
Honorato Sobrinho, Abraão Allen Rego, Amália Cinthia Meneses do Garcia, Ana Claudia M. de Amorim Fernandes, Daniele Pimentel Cruz, Thaís Medeiros Costa, Ticiana Cabral da Medeiros, Aldo Cunha |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Araújo Filho, Irami Honorato Sobrinho, Abraão Allen Rego, Amália Cinthia Meneses do Garcia, Ana Claudia M. de Amorim Fernandes, Daniele Pimentel Cruz, Thaís Medeiros Costa, Ticiana Cabral da Medeiros, Aldo Cunha |
dc.subject.por.fl_str_mv |
Pneumoperitoneum Carbon Dioxide Sepsis Acidosis Leucocytes |
topic |
Pneumoperitoneum Carbon Dioxide Sepsis Acidosis Leucocytes |
description |
Laparoscopic surgery is associated with reduced surgical trauma, and less acute phase response, as compared with open surgery. Cytokines are important regulators of the biological response to surgical and anesthetic stress. The aim of this study was to determine if CO2 pneumoperitoneum would change cytokine expression, gas parameters and leukocyte count in septic rats. Methods: Wistar rats were randomly assigned to five groups: control (anesthesia only), laparotomy, CO2 pneumoperitoneum, cecum ligation and puncture by laparotomy, and laparoscopic cecum ligation and puncture. After 30 min of the procedures, arterial blood samples were obtained to determine leukocytes subpopulations by hemocytometer. TNFα, IL-1β, IL-6 were determined in intraperitoneal fluid (by ELISA). Gas parameters were measured on arterial blood, intraperitoneal and subperitoneal exsudates. Results: Peritoneal TNFα, IL-1β and IL-6 concentrations were lower in pneumoperitoneum rats than in all other groups (p<0.05). TNFα, IL-1β and IL-6 expression was lower in the laparoscopic than in laparotomic sepsis (p<0.05). Rats from laparoscopic cecum ligation and puncture group developed significant hypercarbic acidosis in blood and subperitoneal fluid when compared to open procedure group. Total white blood cells and lymphocytes were significantly lower in laparoscopic cecum ligation and puncture rats than in the laparotomic (p<0.01). Nevertheless, the laparotomic cecum ligation rats had a significant increase in blood neutrophils and eosinophils when compared with controls (p<0.05). Conclusions: This study demonstrates that the CO2 pneumoperitoneum reduced the inflammatory response in an animal model of peritonitis with respect to intraperitoneal cytokines, white blood cell count and clinical correlates of sepsis. The pneumoperitoneum produced hypercarbic acidosis in septic animals |
publishDate |
2006 |
dc.date.issued.fl_str_mv |
2006 |
dc.date.accessioned.fl_str_mv |
2013-09-30T16:23:59Z |
dc.date.available.fl_str_mv |
2013-09-30T16:23:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ARAÚJO FILHO, Irami ; HONORATO SOBRINHO, Abraão Allen ; REGO, Amália Cinthia Meneses do ; GARCIA, Ana Claudia M. de Amorim ; FERNANDES, Daniele Pimentel ; CRUZ, Thaís Medeiros ; COSTA, Ticiana Cabral da ; MEDEIROS, Aldo Cunha. Influence of laparoscopy and laparotomy on gasometry, leukocytes and cytokines in a rat abdominal sepsis model. Acta Cirúrgica Brasileira , Brasil, v. 21, n.2, p. 74-79, 2006. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502006000200004> Acesso em: 20 set. 2013. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/jspui/handle/1/6299 |
dc.identifier.issn.none.fl_str_mv |
1678-2674 |
identifier_str_mv |
ARAÚJO FILHO, Irami ; HONORATO SOBRINHO, Abraão Allen ; REGO, Amália Cinthia Meneses do ; GARCIA, Ana Claudia M. de Amorim ; FERNANDES, Daniele Pimentel ; CRUZ, Thaís Medeiros ; COSTA, Ticiana Cabral da ; MEDEIROS, Aldo Cunha. Influence of laparoscopy and laparotomy on gasometry, leukocytes and cytokines in a rat abdominal sepsis model. Acta Cirúrgica Brasileira , Brasil, v. 21, n.2, p. 74-79, 2006. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502006000200004> Acesso em: 20 set. 2013. 1678-2674 |
url |
https://repositorio.ufrn.br/jspui/handle/1/6299 |
dc.language.iso.fl_str_mv |
eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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UFRN |
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Repositório Institucional da UFRN |
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Repositório Institucional da UFRN |
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