Design of Magnetic Polymeric Particles as a Stimulus-Responsive System for Gastric Antimicrobial Therapy
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/jspui/handle/123456789/28791 https://doi.org/10.1208/s12249-016-0673-1 |
Resumo: | The treatment of peptic ulcers induced by H. pylori remains challenging due to the deep mucous layer location of bacteria preventing antimicrobial drug access. The present work aimed to design and evaluate in vitro dual responsive (both pH and magnetic field-sensitive) polymeric magnetic particles loaded with amoxicillin as a smart drug carrier for deep mucous layer penetration and in situ drug release. Magnetite particles were produced by the co-precipitation method and subsequently coated with the Eudragit®S100 and amoxicillin by using the spray-drying technique. The physicochemical characterization of the obtained particles was carried out by optical and scanning electron microscopy, X-ray powder diffraction, Fourier transform infrared spectroscopy, nitrogen adsorption/desorption isotherms, and vibrating sample magnetometry. Additionally, drug release tests and antibacterial activity tests were evaluated in vitro. Microparticles presented 17.2 ± 0.4 μm in size and their final composition was 4.3 ± 1.5% of amoxicillin, 87.0 ± 2.3% of Eudragit, and 9.0 ± 0.3% of magnetite. They were both pH and magnetic field responsive while presenting antimicrobial activity. On one side, magnetic field responsiveness of particles is expected to prompt them to reach bacterium niche in deep mucous layer by means of magnetic forces. On the other side, pH responsiveness is expected to enable drug release in the neutral pH of the deep mucous layer, preventing undesired delivery in the acidic gastric lumen. Smart microparticles were designed presenting both pH and magnetic field responsiveness as well as antimicrobial activity. These may be promising assets for peptic ulcer treatment. |
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Carriço, Artur da SilvaSilva-Freitas, Erica L.Pontes, Thales R. F.Araújo-Neto, Rafael P.Damasceno, Ítalo H. M.Silva, Kátia L.Carvalho, Juliana F.Medeiros, Aldo C.Silva, Rodolfo B.Silva, Amanda K. A.Morales, Marco A.Egito, Eryvaldo S. T.Dantas, Ana L.2020-04-16T13:10:35Z2020-04-16T13:10:35Z2016-12-13CARRIÇO, Artur da Silva et al. Design of Magnetic Polymeric Particles as a Stimulus-Responsive System for Gastric Antimicrobial Therapy. AAPS PharmSciTech, v. 17, p. 1-11, 2016. ISSN 1530-9932 versão online. DOI https://doi.org/10.1208/s12249-016-0673-1. Disponível em: https://link.springer.com/article/10.1208/s12249-016-0673-1. Acesso em: 8 abr. 2020.1530-9932https://repositorio.ufrn.br/jspui/handle/123456789/28791https://doi.org/10.1208/s12249-016-0673-1Springer Nature Switzerland AGMagnetic polymeric particlesGastric antimicrobial therapyDesign of Magnetic Polymeric Particles as a Stimulus-Responsive System for Gastric Antimicrobial Therapyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleThe treatment of peptic ulcers induced by H. pylori remains challenging due to the deep mucous layer location of bacteria preventing antimicrobial drug access. The present work aimed to design and evaluate in vitro dual responsive (both pH and magnetic field-sensitive) polymeric magnetic particles loaded with amoxicillin as a smart drug carrier for deep mucous layer penetration and in situ drug release. Magnetite particles were produced by the co-precipitation method and subsequently coated with the Eudragit®S100 and amoxicillin by using the spray-drying technique. The physicochemical characterization of the obtained particles was carried out by optical and scanning electron microscopy, X-ray powder diffraction, Fourier transform infrared spectroscopy, nitrogen adsorption/desorption isotherms, and vibrating sample magnetometry. Additionally, drug release tests and antibacterial activity tests were evaluated in vitro. Microparticles presented 17.2 ± 0.4 μm in size and their final composition was 4.3 ± 1.5% of amoxicillin, 87.0 ± 2.3% of Eudragit, and 9.0 ± 0.3% of magnetite. They were both pH and magnetic field responsive while presenting antimicrobial activity. On one side, magnetic field responsiveness of particles is expected to prompt them to reach bacterium niche in deep mucous layer by means of magnetic forces. On the other side, pH responsiveness is expected to enable drug release in the neutral pH of the deep mucous layer, preventing undesired delivery in the acidic gastric lumen. Smart microparticles were designed presenting both pH and magnetic field responsiveness as well as antimicrobial activity. These may be promising assets for peptic ulcer treatment.engreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNinfo:eu-repo/semantics/openAccessORIGINALDesignOfMagneticPolymericParticlesAsAStimulus-REsPonsiveSystem_carrico_2016.pdfDesignOfMagneticPolymericParticlesAsAStimulus-REsPonsiveSystem_carrico_2016.pdfapplication/pdf1512621https://repositorio.ufrn.br/bitstream/123456789/28791/1/DesignOfMagneticPolymericParticlesAsAStimulus-REsPonsiveSystem_carrico_2016.pdf7c103756e9f2bcc0386b4879803daedcMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/28791/2/license.txte9597aa2854d128fd968be5edc8a28d9MD52TEXTDesignOfMagneticPolymericParticlesAsAStimulus-REsPonsiveSystem_carrico_2016.pdf.txtDesignOfMagneticPolymericParticlesAsAStimulus-REsPonsiveSystem_carrico_2016.pdf.txtExtracted texttext/plain44022https://repositorio.ufrn.br/bitstream/123456789/28791/3/DesignOfMagneticPolymericParticlesAsAStimulus-REsPonsiveSystem_carrico_2016.pdf.txt7f23101315bc6b6c6b343a7dfac06b16MD53THUMBNAILDesignOfMagneticPolymericParticlesAsAStimulus-REsPonsiveSystem_carrico_2016.pdf.jpgDesignOfMagneticPolymericParticlesAsAStimulus-REsPonsiveSystem_carrico_2016.pdf.jpgGenerated Thumbnailimage/jpeg1793https://repositorio.ufrn.br/bitstream/123456789/28791/4/DesignOfMagneticPolymericParticlesAsAStimulus-REsPonsiveSystem_carrico_2016.pdf.jpga3645cdd6d3c5dc8093de356e0b052deMD54123456789/287912020-05-05 01:41:35.878oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2020-05-05T04:41:35Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
Design of Magnetic Polymeric Particles as a Stimulus-Responsive System for Gastric Antimicrobial Therapy |
title |
Design of Magnetic Polymeric Particles as a Stimulus-Responsive System for Gastric Antimicrobial Therapy |
spellingShingle |
Design of Magnetic Polymeric Particles as a Stimulus-Responsive System for Gastric Antimicrobial Therapy Carriço, Artur da Silva Magnetic polymeric particles Gastric antimicrobial therapy |
title_short |
Design of Magnetic Polymeric Particles as a Stimulus-Responsive System for Gastric Antimicrobial Therapy |
title_full |
Design of Magnetic Polymeric Particles as a Stimulus-Responsive System for Gastric Antimicrobial Therapy |
title_fullStr |
Design of Magnetic Polymeric Particles as a Stimulus-Responsive System for Gastric Antimicrobial Therapy |
title_full_unstemmed |
Design of Magnetic Polymeric Particles as a Stimulus-Responsive System for Gastric Antimicrobial Therapy |
title_sort |
Design of Magnetic Polymeric Particles as a Stimulus-Responsive System for Gastric Antimicrobial Therapy |
author |
Carriço, Artur da Silva |
author_facet |
Carriço, Artur da Silva Silva-Freitas, Erica L. Pontes, Thales R. F. Araújo-Neto, Rafael P. Damasceno, Ítalo H. M. Silva, Kátia L. Carvalho, Juliana F. Medeiros, Aldo C. Silva, Rodolfo B. Silva, Amanda K. A. Morales, Marco A. Egito, Eryvaldo S. T. Dantas, Ana L. |
author_role |
author |
author2 |
Silva-Freitas, Erica L. Pontes, Thales R. F. Araújo-Neto, Rafael P. Damasceno, Ítalo H. M. Silva, Kátia L. Carvalho, Juliana F. Medeiros, Aldo C. Silva, Rodolfo B. Silva, Amanda K. A. Morales, Marco A. Egito, Eryvaldo S. T. Dantas, Ana L. |
author2_role |
author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Carriço, Artur da Silva Silva-Freitas, Erica L. Pontes, Thales R. F. Araújo-Neto, Rafael P. Damasceno, Ítalo H. M. Silva, Kátia L. Carvalho, Juliana F. Medeiros, Aldo C. Silva, Rodolfo B. Silva, Amanda K. A. Morales, Marco A. Egito, Eryvaldo S. T. Dantas, Ana L. |
dc.subject.por.fl_str_mv |
Magnetic polymeric particles Gastric antimicrobial therapy |
topic |
Magnetic polymeric particles Gastric antimicrobial therapy |
description |
The treatment of peptic ulcers induced by H. pylori remains challenging due to the deep mucous layer location of bacteria preventing antimicrobial drug access. The present work aimed to design and evaluate in vitro dual responsive (both pH and magnetic field-sensitive) polymeric magnetic particles loaded with amoxicillin as a smart drug carrier for deep mucous layer penetration and in situ drug release. Magnetite particles were produced by the co-precipitation method and subsequently coated with the Eudragit®S100 and amoxicillin by using the spray-drying technique. The physicochemical characterization of the obtained particles was carried out by optical and scanning electron microscopy, X-ray powder diffraction, Fourier transform infrared spectroscopy, nitrogen adsorption/desorption isotherms, and vibrating sample magnetometry. Additionally, drug release tests and antibacterial activity tests were evaluated in vitro. Microparticles presented 17.2 ± 0.4 μm in size and their final composition was 4.3 ± 1.5% of amoxicillin, 87.0 ± 2.3% of Eudragit, and 9.0 ± 0.3% of magnetite. They were both pH and magnetic field responsive while presenting antimicrobial activity. On one side, magnetic field responsiveness of particles is expected to prompt them to reach bacterium niche in deep mucous layer by means of magnetic forces. On the other side, pH responsiveness is expected to enable drug release in the neutral pH of the deep mucous layer, preventing undesired delivery in the acidic gastric lumen. Smart microparticles were designed presenting both pH and magnetic field responsiveness as well as antimicrobial activity. These may be promising assets for peptic ulcer treatment. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-12-13 |
dc.date.accessioned.fl_str_mv |
2020-04-16T13:10:35Z |
dc.date.available.fl_str_mv |
2020-04-16T13:10:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CARRIÇO, Artur da Silva et al. Design of Magnetic Polymeric Particles as a Stimulus-Responsive System for Gastric Antimicrobial Therapy. AAPS PharmSciTech, v. 17, p. 1-11, 2016. ISSN 1530-9932 versão online. DOI https://doi.org/10.1208/s12249-016-0673-1. Disponível em: https://link.springer.com/article/10.1208/s12249-016-0673-1. Acesso em: 8 abr. 2020. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/jspui/handle/123456789/28791 |
dc.identifier.issn.none.fl_str_mv |
1530-9932 |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1208/s12249-016-0673-1 |
identifier_str_mv |
CARRIÇO, Artur da Silva et al. Design of Magnetic Polymeric Particles as a Stimulus-Responsive System for Gastric Antimicrobial Therapy. AAPS PharmSciTech, v. 17, p. 1-11, 2016. ISSN 1530-9932 versão online. DOI https://doi.org/10.1208/s12249-016-0673-1. Disponível em: https://link.springer.com/article/10.1208/s12249-016-0673-1. Acesso em: 8 abr. 2020. 1530-9932 |
url |
https://repositorio.ufrn.br/jspui/handle/123456789/28791 https://doi.org/10.1208/s12249-016-0673-1 |
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eng |
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eng |
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openAccess |
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Springer Nature Switzerland AG |
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Springer Nature Switzerland AG |
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