Preparative chromatography of xylanase using expanded bed adsorption

Detalhes bibliográficos
Autor(a) principal: Santos, Everaldo Silvino dos
Data de Publicação: 2002
Outros Autores: Guirardello, Reginaldo, Franco, Telma Teixeira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/handle/123456789/32673
Resumo: Expanded bed adsorption was used to purify a marketable xylanase often used in the kraft pulp bleaching process. Experiments in packed and expanded beds were carried out mainly to study the adsorption of xylanase on to a cationic adsorbent (Streamline SP) in the presence of cells. In order to study the presence of cells, a Bacillus pumilus mass (5% wet mass) was mixed with the enzyme extract and submitted to an expanded bed adsorption system. One xylanase was purified to homogeneity in the packed bed. However, the 5% cell content hampered purification
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spelling Santos, Everaldo Silvino dosGuirardello, ReginaldoFranco, Telma Teixeira2021-06-14T14:57:15Z2021-06-14T14:57:15Z2002-01SANTOS, E. S.; GUIRARDELLO, R.; FRANCO, T. T.. Preparative chromatography of xylanase using expanded bed adsorption. Journal of Chromatography (Print), Nova Iorque, v. 944, n.1-2, p. 217-224, 2002. Disponível em: https://www.sciencedirect.com/science/article/pii/S0021967301013681?via%3Dihub. Acesso em: 20 abr. 2021. https://doi.org/10.1016/S0021-9673(01)01368-10021-9673https://repositorio.ufrn.br/handle/123456789/3267310.1016/S0021-9673(01)01368-1ElsevierAttribution 3.0 Brazilhttp://creativecommons.org/licenses/by/3.0/br/info:eu-repo/semantics/openAccessKraft pulpExpanded bed adsorptionAdsorptionPreparative chromatographyXylanaseEnzymesPreparative chromatography of xylanase using expanded bed adsorptioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleExpanded bed adsorption was used to purify a marketable xylanase often used in the kraft pulp bleaching process. Experiments in packed and expanded beds were carried out mainly to study the adsorption of xylanase on to a cationic adsorbent (Streamline SP) in the presence of cells. In order to study the presence of cells, a Bacillus pumilus mass (5% wet mass) was mixed with the enzyme extract and submitted to an expanded bed adsorption system. One xylanase was purified to homogeneity in the packed bed. However, the 5% cell content hampered purificationengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALPreparativeChromatographyXylanase_Santos_2002.pdfPreparativeChromatographyXylanase_Santos_2002.pdfapplication/pdf323339https://repositorio.ufrn.br/bitstream/123456789/32673/1/PreparativeChromatographyXylanase_Santos_2002.pdf1083e8bd0d0ef81e00533872aff926b1MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.ufrn.br/bitstream/123456789/32673/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/32673/3/license.txte9597aa2854d128fd968be5edc8a28d9MD53123456789/326732021-06-14 11:57:16.288oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2021-06-14T14:57:16Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.pt_BR.fl_str_mv Preparative chromatography of xylanase using expanded bed adsorption
title Preparative chromatography of xylanase using expanded bed adsorption
spellingShingle Preparative chromatography of xylanase using expanded bed adsorption
Santos, Everaldo Silvino dos
Kraft pulp
Expanded bed adsorption
Adsorption
Preparative chromatography
Xylanase
Enzymes
title_short Preparative chromatography of xylanase using expanded bed adsorption
title_full Preparative chromatography of xylanase using expanded bed adsorption
title_fullStr Preparative chromatography of xylanase using expanded bed adsorption
title_full_unstemmed Preparative chromatography of xylanase using expanded bed adsorption
title_sort Preparative chromatography of xylanase using expanded bed adsorption
author Santos, Everaldo Silvino dos
author_facet Santos, Everaldo Silvino dos
Guirardello, Reginaldo
Franco, Telma Teixeira
author_role author
author2 Guirardello, Reginaldo
Franco, Telma Teixeira
author2_role author
author
dc.contributor.author.fl_str_mv Santos, Everaldo Silvino dos
Guirardello, Reginaldo
Franco, Telma Teixeira
dc.subject.por.fl_str_mv Kraft pulp
Expanded bed adsorption
Adsorption
Preparative chromatography
Xylanase
Enzymes
topic Kraft pulp
Expanded bed adsorption
Adsorption
Preparative chromatography
Xylanase
Enzymes
description Expanded bed adsorption was used to purify a marketable xylanase often used in the kraft pulp bleaching process. Experiments in packed and expanded beds were carried out mainly to study the adsorption of xylanase on to a cationic adsorbent (Streamline SP) in the presence of cells. In order to study the presence of cells, a Bacillus pumilus mass (5% wet mass) was mixed with the enzyme extract and submitted to an expanded bed adsorption system. One xylanase was purified to homogeneity in the packed bed. However, the 5% cell content hampered purification
publishDate 2002
dc.date.issued.fl_str_mv 2002-01
dc.date.accessioned.fl_str_mv 2021-06-14T14:57:15Z
dc.date.available.fl_str_mv 2021-06-14T14:57:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SANTOS, E. S.; GUIRARDELLO, R.; FRANCO, T. T.. Preparative chromatography of xylanase using expanded bed adsorption. Journal of Chromatography (Print), Nova Iorque, v. 944, n.1-2, p. 217-224, 2002. Disponível em: https://www.sciencedirect.com/science/article/pii/S0021967301013681?via%3Dihub. Acesso em: 20 abr. 2021. https://doi.org/10.1016/S0021-9673(01)01368-1
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/handle/123456789/32673
dc.identifier.issn.none.fl_str_mv 0021-9673
dc.identifier.doi.none.fl_str_mv 10.1016/S0021-9673(01)01368-1
identifier_str_mv SANTOS, E. S.; GUIRARDELLO, R.; FRANCO, T. T.. Preparative chromatography of xylanase using expanded bed adsorption. Journal of Chromatography (Print), Nova Iorque, v. 944, n.1-2, p. 217-224, 2002. Disponível em: https://www.sciencedirect.com/science/article/pii/S0021967301013681?via%3Dihub. Acesso em: 20 abr. 2021. https://doi.org/10.1016/S0021-9673(01)01368-1
0021-9673
10.1016/S0021-9673(01)01368-1
url https://repositorio.ufrn.br/handle/123456789/32673
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution 3.0 Brazil
http://creativecommons.org/licenses/by/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 3.0 Brazil
http://creativecommons.org/licenses/by/3.0/br/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFRN
instname:Universidade Federal do Rio Grande do Norte (UFRN)
instacron:UFRN
instname_str Universidade Federal do Rio Grande do Norte (UFRN)
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