Transcriptional profile of induced and primary neurons reveals new candidate genes for lineage reprogramming
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/jspui/handle/123456789/23672 |
Resumo: | Somatic cells can be directly reprogrammed into neurons through the expression of few transcription factors. However, the precise mechanisms involved in the lineage-conversion are poorly understood. Similarly, it remains unclear how similar lineage-reprogrammed induced neurons (iNs) are to bona fide central nervous system neurons. In this work, we used an unsupervised machine-learning approach to compare the transcriptional profiles of lineage-reprogrammed mouse embryonic fibroblasts (MEFs), mouse embryonic telencephalon neural progenitors and neurons, as well as mouse postnatal cerebral cortex neurons. We show that the transcriptional profile of a subpopulation of lineage-reprogrammed MEFs resembles that of primary neural progenitors and neurons, indicating that the intermediate steps enacted by reprogramming factors within MEFs during the transition to iNs are similar to those observed during primary neuron differentiation. Finally, by comparing the transcriptional profiles of MEFs that undertook a neuronal pathway to that of MEFs adopting a myogenic fate or retaining fibroblast features we identified potential candidates to improve the efficiency for lineage conversion of those cells into neurons. |
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Coelho, Diego MSouza, Sandro José deCosta, Marcos Romualdo2017-07-21T12:05:22Z2017-07-21T12:05:22Z2017-07-21https://repositorio.ufrn.br/jspui/handle/123456789/2367210.19185/matters.201704000019engMattersDirect Lineage ReprogrammingDevelopmental NeurobiologyPrimary NeuronsTranscriptional profile of induced and primary neurons reveals new candidate genes for lineage reprogramminginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSomatic cells can be directly reprogrammed into neurons through the expression of few transcription factors. However, the precise mechanisms involved in the lineage-conversion are poorly understood. Similarly, it remains unclear how similar lineage-reprogrammed induced neurons (iNs) are to bona fide central nervous system neurons. In this work, we used an unsupervised machine-learning approach to compare the transcriptional profiles of lineage-reprogrammed mouse embryonic fibroblasts (MEFs), mouse embryonic telencephalon neural progenitors and neurons, as well as mouse postnatal cerebral cortex neurons. We show that the transcriptional profile of a subpopulation of lineage-reprogrammed MEFs resembles that of primary neural progenitors and neurons, indicating that the intermediate steps enacted by reprogramming factors within MEFs during the transition to iNs are similar to those observed during primary neuron differentiation. Finally, by comparing the transcriptional profiles of MEFs that undertook a neuronal pathway to that of MEFs adopting a myogenic fate or retaining fibroblast features we identified potential candidates to improve the efficiency for lineage conversion of those cells into neurons.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNLICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.ufrn.br/bitstream/123456789/23672/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52ORIGINALMarcosCosta_ICE_2017_Transcriptional profile of induced.pdfMarcosCosta_ICE_2017_Transcriptional profile of induced.pdfArtigo Completoapplication/pdf1392013https://repositorio.ufrn.br/bitstream/123456789/23672/1/MarcosCosta_ICE_2017_Transcriptional%20profile%20of%20induced.pdfe5041134769d25ff07638de77d0638f6MD51TEXTMarcosCosta_ICE_2017_Transcriptional profile of induced.pdf.txtMarcosCosta_ICE_2017_Transcriptional profile of induced.pdf.txtExtracted texttext/plain26350https://repositorio.ufrn.br/bitstream/123456789/23672/5/MarcosCosta_ICE_2017_Transcriptional%20profile%20of%20induced.pdf.txt314590d05eae766edd1b0ed9a283b814MD55THUMBNAILMarcosCosta_ICE_2017_Transcriptional profile of induced.pdf.jpgMarcosCosta_ICE_2017_Transcriptional profile of induced.pdf.jpgIM Thumbnailimage/jpeg7905https://repositorio.ufrn.br/bitstream/123456789/23672/6/MarcosCosta_ICE_2017_Transcriptional%20profile%20of%20induced.pdf.jpg2e30a7a77c2493ed2eff28b423296a7eMD56123456789/236722021-07-09 17:31:49.65oai:https://repositorio.ufrn.br:123456789/23672Tk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo=Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2021-07-09T20:31:49Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
Transcriptional profile of induced and primary neurons reveals new candidate genes for lineage reprogramming |
title |
Transcriptional profile of induced and primary neurons reveals new candidate genes for lineage reprogramming |
spellingShingle |
Transcriptional profile of induced and primary neurons reveals new candidate genes for lineage reprogramming Coelho, Diego M Direct Lineage Reprogramming Developmental Neurobiology Primary Neurons |
title_short |
Transcriptional profile of induced and primary neurons reveals new candidate genes for lineage reprogramming |
title_full |
Transcriptional profile of induced and primary neurons reveals new candidate genes for lineage reprogramming |
title_fullStr |
Transcriptional profile of induced and primary neurons reveals new candidate genes for lineage reprogramming |
title_full_unstemmed |
Transcriptional profile of induced and primary neurons reveals new candidate genes for lineage reprogramming |
title_sort |
Transcriptional profile of induced and primary neurons reveals new candidate genes for lineage reprogramming |
author |
Coelho, Diego M |
author_facet |
Coelho, Diego M Souza, Sandro José de Costa, Marcos Romualdo |
author_role |
author |
author2 |
Souza, Sandro José de Costa, Marcos Romualdo |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Coelho, Diego M Souza, Sandro José de Costa, Marcos Romualdo |
dc.subject.por.fl_str_mv |
Direct Lineage Reprogramming Developmental Neurobiology Primary Neurons |
topic |
Direct Lineage Reprogramming Developmental Neurobiology Primary Neurons |
description |
Somatic cells can be directly reprogrammed into neurons through the expression of few transcription factors. However, the precise mechanisms involved in the lineage-conversion are poorly understood. Similarly, it remains unclear how similar lineage-reprogrammed induced neurons (iNs) are to bona fide central nervous system neurons. In this work, we used an unsupervised machine-learning approach to compare the transcriptional profiles of lineage-reprogrammed mouse embryonic fibroblasts (MEFs), mouse embryonic telencephalon neural progenitors and neurons, as well as mouse postnatal cerebral cortex neurons. We show that the transcriptional profile of a subpopulation of lineage-reprogrammed MEFs resembles that of primary neural progenitors and neurons, indicating that the intermediate steps enacted by reprogramming factors within MEFs during the transition to iNs are similar to those observed during primary neuron differentiation. Finally, by comparing the transcriptional profiles of MEFs that undertook a neuronal pathway to that of MEFs adopting a myogenic fate or retaining fibroblast features we identified potential candidates to improve the efficiency for lineage conversion of those cells into neurons. |
publishDate |
2017 |
dc.date.accessioned.fl_str_mv |
2017-07-21T12:05:22Z |
dc.date.available.fl_str_mv |
2017-07-21T12:05:22Z |
dc.date.issued.fl_str_mv |
2017-07-21 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/jspui/handle/123456789/23672 |
dc.identifier.doi.none.fl_str_mv |
10.19185/matters.201704000019 |
url |
https://repositorio.ufrn.br/jspui/handle/123456789/23672 |
identifier_str_mv |
10.19185/matters.201704000019 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Matters |
publisher.none.fl_str_mv |
Matters |
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reponame:Repositório Institucional da UFRN instname:Universidade Federal do Rio Grande do Norte (UFRN) instacron:UFRN |
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UFRN |
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UFRN |
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Repositório Institucional da UFRN |
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