Transcriptional Changes That Characterize the Immune Reactions of Leprosy
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/jspui/handle/123456789/19273 |
Resumo: | Background: Leprosy morbidity is increased by 2 pathologic immune reactions, reversal reaction (RR) and erythema nodosum leprosum (ENL). Methods: To discover host factors related to immune reactions, global transcriptional profiles of peripheral blood mononuclear cells were compared between 11 RR, 11 ENL, and 19 matched control patients, with confirmation by quantitative polymerase chain reaction. Encoded proteins were investigated in skin biopsy specimens by means of immunohistochemistry. Results: There were 275 genes differentially expressed in RR and 517 differentially expressed in ENL on the microarray. Pathway analysis showed immunity-related pathways represented in RR and ENL transcriptional profiles, with the “complement and coagulation” pathway common to both. Interferon γ was identified as a significant upstream regulator of the expression changes for RR and ENL. Immunohistochemical staining of skin lesions showed increased C1q in both RR and ENL. Conclusions: These data suggest a previously underrecognized role for complement in the pathogenesis of both RR and ENL, and we propose new hypotheses for reaction pathogenesis. |
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Dupnik, Kathryn M.Bair, Thomas B.Maia, Andressa O.Amorim, Francianne M.Costa, Marcos RomualdoKeesen, Tatjana S. L.Valverde, Joanna G.Queiroz, Maria do Carmo A. P.Medeiros, Lúcio L.Lucena, Nelly L. deWilson, Mary E.Nobre, Mauricio L.Johnson Jr, Warren D.Jeronimo, Selma M. B.2015-09-25T19:57:56Z2015-09-25T19:57:56Z2014DUPNIK, Kathryn M. et al. Transcriptional Changes That Characterize the Immune Reactions of Leprosy. Journal of Infectious Diseases, v. 211, n. 10. 14 nov. 2014.https://repositorio.ufrn.br/jspui/handle/123456789/19273Background: Leprosy morbidity is increased by 2 pathologic immune reactions, reversal reaction (RR) and erythema nodosum leprosum (ENL). Methods: To discover host factors related to immune reactions, global transcriptional profiles of peripheral blood mononuclear cells were compared between 11 RR, 11 ENL, and 19 matched control patients, with confirmation by quantitative polymerase chain reaction. Encoded proteins were investigated in skin biopsy specimens by means of immunohistochemistry. Results: There were 275 genes differentially expressed in RR and 517 differentially expressed in ENL on the microarray. Pathway analysis showed immunity-related pathways represented in RR and ENL transcriptional profiles, with the “complement and coagulation” pathway common to both. Interferon γ was identified as a significant upstream regulator of the expression changes for RR and ENL. Immunohistochemical staining of skin lesions showed increased C1q in both RR and ENL. Conclusions: These data suggest a previously underrecognized role for complement in the pathogenesis of both RR and ENL, and we propose new hypotheses for reaction pathogenesis.Background: Leprosy morbidity is increased by 2 pathologic immune reactions, reversal reaction (RR) and erythema nodosum leprosum (ENL). Methods: To discover host factors related to immune reactions, global transcriptional profiles of peripheral blood mononuclear cells were compared between 11 RR, 11 ENL, and 19 matched control patients, with confirmation by quantitative polymerase chain reaction. Encoded proteins were investigated in skin biopsy specimens by means of immunohistochemistry. Results: There were 275 genes differentially expressed in RR and 517 differentially expressed in ENL on the microarray. Pathway analysis showed immunity-related pathways represented in RR and ENL transcriptional profiles, with the “complement and coagulation” pathway common to both. Interferon γ was identified as a significant upstream regulator of the expression changes for RR and ENL. Immunohistochemical staining of skin lesions showed increased C1q in both RR and ENL. Conclusions: These data suggest a previously underrecognized role for complement in the pathogenesis of both RR and ENL, and we propose new hypotheses for reaction pathogenesis.engUniversidade Federal do Rio Grande do NorteUFRNBrasilleprosyreversal reactionerythema nodosum leprosumcomplementTranscriptional Changes That Characterize the Immune Reactions of Leprosyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALMarcosCosta_ICE_ 2014_J Infect Dis_Transcriptional Changes That Characterize the Immune Reactions of Leprosy.pdfMarcosCosta_ICE_ 2014_J Infect Dis_Transcriptional Changes That Characterize the Immune Reactions of Leprosy.pdfapplication/pdf556960https://repositorio.ufrn.br/bitstream/123456789/19273/1/MarcosCosta_ICE_%202014_J%20Infect%20Dis_Transcriptional%20Changes%20That%20Characterize%20the%20Immune%20Reactions%20of%20Leprosy.pdf0e3b39eea6848d351b4d4da612e72e21MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81526https://repositorio.ufrn.br/bitstream/123456789/19273/2/license.txt28835e092517bf3ceacc478b9f2a16d5MD52TEXTMarcosCosta_ICE_ 2014_J Infect Dis_Transcriptional Changes That Characterize the Immune Reactions of Leprosy.pdf.txtMarcosCosta_ICE_ 2014_J Infect Dis_Transcriptional Changes That Characterize the Immune Reactions of Leprosy.pdf.txtExtracted texttext/plain68660https://repositorio.ufrn.br/bitstream/123456789/19273/7/MarcosCosta_ICE_%202014_J%20Infect%20Dis_Transcriptional%20Changes%20That%20Characterize%20the%20Immune%20Reactions%20of%20Leprosy.pdf.txt0489b2f7dba17f8c03a553e7aefca8f5MD57THUMBNAILMarcosCosta_ICE_ 2014_J Infect Dis_Transcriptional Changes That Characterize the Immune Reactions of Leprosy.pdf.jpgMarcosCosta_ICE_ 2014_J Infect Dis_Transcriptional Changes That Characterize the Immune Reactions of Leprosy.pdf.jpgIM Thumbnailimage/jpeg8917https://repositorio.ufrn.br/bitstream/123456789/19273/8/MarcosCosta_ICE_%202014_J%20Infect%20Dis_Transcriptional%20Changes%20That%20Characterize%20the%20Immune%20Reactions%20of%20Leprosy.pdf.jpg27c146c11579fdb6b8fd8e791e1a6b21MD58123456789/192732021-07-09 17:42:45.929oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2021-07-09T20:42:45Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
Transcriptional Changes That Characterize the Immune Reactions of Leprosy |
title |
Transcriptional Changes That Characterize the Immune Reactions of Leprosy |
spellingShingle |
Transcriptional Changes That Characterize the Immune Reactions of Leprosy Dupnik, Kathryn M. leprosy reversal reaction erythema nodosum leprosum complement |
title_short |
Transcriptional Changes That Characterize the Immune Reactions of Leprosy |
title_full |
Transcriptional Changes That Characterize the Immune Reactions of Leprosy |
title_fullStr |
Transcriptional Changes That Characterize the Immune Reactions of Leprosy |
title_full_unstemmed |
Transcriptional Changes That Characterize the Immune Reactions of Leprosy |
title_sort |
Transcriptional Changes That Characterize the Immune Reactions of Leprosy |
author |
Dupnik, Kathryn M. |
author_facet |
Dupnik, Kathryn M. Bair, Thomas B. Maia, Andressa O. Amorim, Francianne M. Costa, Marcos Romualdo Keesen, Tatjana S. L. Valverde, Joanna G. Queiroz, Maria do Carmo A. P. Medeiros, Lúcio L. Lucena, Nelly L. de Wilson, Mary E. Nobre, Mauricio L. Johnson Jr, Warren D. Jeronimo, Selma M. B. |
author_role |
author |
author2 |
Bair, Thomas B. Maia, Andressa O. Amorim, Francianne M. Costa, Marcos Romualdo Keesen, Tatjana S. L. Valverde, Joanna G. Queiroz, Maria do Carmo A. P. Medeiros, Lúcio L. Lucena, Nelly L. de Wilson, Mary E. Nobre, Mauricio L. Johnson Jr, Warren D. Jeronimo, Selma M. B. |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Dupnik, Kathryn M. Bair, Thomas B. Maia, Andressa O. Amorim, Francianne M. Costa, Marcos Romualdo Keesen, Tatjana S. L. Valverde, Joanna G. Queiroz, Maria do Carmo A. P. Medeiros, Lúcio L. Lucena, Nelly L. de Wilson, Mary E. Nobre, Mauricio L. Johnson Jr, Warren D. Jeronimo, Selma M. B. |
dc.subject.por.fl_str_mv |
leprosy reversal reaction erythema nodosum leprosum complement |
topic |
leprosy reversal reaction erythema nodosum leprosum complement |
description |
Background: Leprosy morbidity is increased by 2 pathologic immune reactions, reversal reaction (RR) and erythema nodosum leprosum (ENL). Methods: To discover host factors related to immune reactions, global transcriptional profiles of peripheral blood mononuclear cells were compared between 11 RR, 11 ENL, and 19 matched control patients, with confirmation by quantitative polymerase chain reaction. Encoded proteins were investigated in skin biopsy specimens by means of immunohistochemistry. Results: There were 275 genes differentially expressed in RR and 517 differentially expressed in ENL on the microarray. Pathway analysis showed immunity-related pathways represented in RR and ENL transcriptional profiles, with the “complement and coagulation” pathway common to both. Interferon γ was identified as a significant upstream regulator of the expression changes for RR and ENL. Immunohistochemical staining of skin lesions showed increased C1q in both RR and ENL. Conclusions: These data suggest a previously underrecognized role for complement in the pathogenesis of both RR and ENL, and we propose new hypotheses for reaction pathogenesis. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014 |
dc.date.accessioned.fl_str_mv |
2015-09-25T19:57:56Z |
dc.date.available.fl_str_mv |
2015-09-25T19:57:56Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
DUPNIK, Kathryn M. et al. Transcriptional Changes That Characterize the Immune Reactions of Leprosy. Journal of Infectious Diseases, v. 211, n. 10. 14 nov. 2014. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/jspui/handle/123456789/19273 |
identifier_str_mv |
DUPNIK, Kathryn M. et al. Transcriptional Changes That Characterize the Immune Reactions of Leprosy. Journal of Infectious Diseases, v. 211, n. 10. 14 nov. 2014. |
url |
https://repositorio.ufrn.br/jspui/handle/123456789/19273 |
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eng |
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eng |
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Universidade Federal do Rio Grande do Norte |
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UFRN |
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Brasil |
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Universidade Federal do Rio Grande do Norte |
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Universidade Federal do Rio Grande do Norte (UFRN) |
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UFRN |
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UFRN |
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Repositório Institucional da UFRN |
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Repositório Institucional da UFRN |
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