Effect of FGF-2 and sciatic nerve grafting on ChAT expression in dorsal root ganglia neurons of spinal cord transected rats
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/handle/123456789/31695 |
Resumo: | Neurotrophic factors and peripheral nerves are known to be good substrates for bridging CNS trauma. The involvement of fibroblast growth factor-2 (FGF-2) activation in the dorsal root ganglion (DRG) was examined following spinal cord injury in the rat. We evaluated whether FGF-2 increases the ability of a sciatic nerve graft to enhance neuronal plasticity, in a gap promoted by complete transection of the spinal cord. The rats were subjected to a 4 mm-long gap at low thoracic level and were repaired with saline (Saline or control group, n = 10), or fragment of the sciatic nerve (Nerve group, n = 10), or fragment of the sciatic nerve to which FGF-2 (Nerve + FGF-2 group, n = 10) had been added immediately after lesion. The effects of the FGF-2 and fragment of the sciatic nerve grafts on neuronal plasticity were investigated using choline acetyl transferase (ChAT)-immunoreactivity of neurons in the dorsal root ganglion after 8 weeks. Preservation of the area and diameter of neuronal cell bodies in dorsal root ganglion (DRG) was seen in animals treated with the sciatic nerve, an effect enhanced by the addition of FGF-2. Thus, the addition of exogenous FGF-2 to a sciatic nerve fragment grafted in a gap of the rat spinal cord submitted to complete transection was able to improve neuroprotection in the DRG. The results emphasized that the manipulation of the microenvironment in the wound might amplify the regenerative capacity of peripheral neurons |
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Guzen, Fausto PierdonáAraújo, Dayane Pessoa deLucena, Eudes Euler de SouzaMorais, Hécio Henrique Araújo deCavalcanti, José Rodolfo Lopes de PaivaNascimento Junior, Expedito Silva doCosta, Miriam Stela Maris de OliveiraCavalcante, Jeferson de Souza2021-03-05T11:06:24Z2021-03-05T11:06:24Z2016-03GUZEN, Fausto Pierdoná; ARAÚJO, Dayane Pessoa de; LUCENA, Eudes Euler de Souza; MORAIS, Hécio Henrique Araújo de; CAVALCANTI, José Rodolfo Lopes de Paiva; NASCIMENTO JUNIOR, Expedito Silva do; COSTA, Miriam Stela Maris de Oliveira; CAVALCANTE, Jeferson de Souza. Effect of FGF-2 and sciatic nerve grafting on ChAT expression in dorsal root ganglia neurons of spinal cord transected rats. Neuroscience Letters, [s. l.], v. 616, p. 43-48, mar. 2016. Elsevier BV. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S0304394015301130?via%3Dihub#!. Acesso em: 23 jul. 2020. http://dx.doi.org/10.1016/j.neulet.2015.08.0430304-3940 (print)https://repositorio.ufrn.br/handle/123456789/3169510.1016/j.neulet.2015.08.043ElsevierAttribution 3.0 Brazilhttp://creativecommons.org/licenses/by/3.0/br/info:eu-repo/semantics/openAccessDorsal root gangliaFibroblastic growth factor-2NeuroprotectionSciatic nerve graftSpinal cordEffect of FGF-2 and sciatic nerve grafting on ChAT expression in dorsal root ganglia neurons of spinal cord transected ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleNeurotrophic factors and peripheral nerves are known to be good substrates for bridging CNS trauma. The involvement of fibroblast growth factor-2 (FGF-2) activation in the dorsal root ganglion (DRG) was examined following spinal cord injury in the rat. We evaluated whether FGF-2 increases the ability of a sciatic nerve graft to enhance neuronal plasticity, in a gap promoted by complete transection of the spinal cord. The rats were subjected to a 4 mm-long gap at low thoracic level and were repaired with saline (Saline or control group, n = 10), or fragment of the sciatic nerve (Nerve group, n = 10), or fragment of the sciatic nerve to which FGF-2 (Nerve + FGF-2 group, n = 10) had been added immediately after lesion. The effects of the FGF-2 and fragment of the sciatic nerve grafts on neuronal plasticity were investigated using choline acetyl transferase (ChAT)-immunoreactivity of neurons in the dorsal root ganglion after 8 weeks. Preservation of the area and diameter of neuronal cell bodies in dorsal root ganglion (DRG) was seen in animals treated with the sciatic nerve, an effect enhanced by the addition of FGF-2. Thus, the addition of exogenous FGF-2 to a sciatic nerve fragment grafted in a gap of the rat spinal cord submitted to complete transection was able to improve neuroprotection in the DRG. 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dc.title.pt_BR.fl_str_mv |
Effect of FGF-2 and sciatic nerve grafting on ChAT expression in dorsal root ganglia neurons of spinal cord transected rats |
title |
Effect of FGF-2 and sciatic nerve grafting on ChAT expression in dorsal root ganglia neurons of spinal cord transected rats |
spellingShingle |
Effect of FGF-2 and sciatic nerve grafting on ChAT expression in dorsal root ganglia neurons of spinal cord transected rats Guzen, Fausto Pierdoná Dorsal root ganglia Fibroblastic growth factor-2 Neuroprotection Sciatic nerve graft Spinal cord |
title_short |
Effect of FGF-2 and sciatic nerve grafting on ChAT expression in dorsal root ganglia neurons of spinal cord transected rats |
title_full |
Effect of FGF-2 and sciatic nerve grafting on ChAT expression in dorsal root ganglia neurons of spinal cord transected rats |
title_fullStr |
Effect of FGF-2 and sciatic nerve grafting on ChAT expression in dorsal root ganglia neurons of spinal cord transected rats |
title_full_unstemmed |
Effect of FGF-2 and sciatic nerve grafting on ChAT expression in dorsal root ganglia neurons of spinal cord transected rats |
title_sort |
Effect of FGF-2 and sciatic nerve grafting on ChAT expression in dorsal root ganglia neurons of spinal cord transected rats |
author |
Guzen, Fausto Pierdoná |
author_facet |
Guzen, Fausto Pierdoná Araújo, Dayane Pessoa de Lucena, Eudes Euler de Souza Morais, Hécio Henrique Araújo de Cavalcanti, José Rodolfo Lopes de Paiva Nascimento Junior, Expedito Silva do Costa, Miriam Stela Maris de Oliveira Cavalcante, Jeferson de Souza |
author_role |
author |
author2 |
Araújo, Dayane Pessoa de Lucena, Eudes Euler de Souza Morais, Hécio Henrique Araújo de Cavalcanti, José Rodolfo Lopes de Paiva Nascimento Junior, Expedito Silva do Costa, Miriam Stela Maris de Oliveira Cavalcante, Jeferson de Souza |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Guzen, Fausto Pierdoná Araújo, Dayane Pessoa de Lucena, Eudes Euler de Souza Morais, Hécio Henrique Araújo de Cavalcanti, José Rodolfo Lopes de Paiva Nascimento Junior, Expedito Silva do Costa, Miriam Stela Maris de Oliveira Cavalcante, Jeferson de Souza |
dc.subject.por.fl_str_mv |
Dorsal root ganglia Fibroblastic growth factor-2 Neuroprotection Sciatic nerve graft Spinal cord |
topic |
Dorsal root ganglia Fibroblastic growth factor-2 Neuroprotection Sciatic nerve graft Spinal cord |
description |
Neurotrophic factors and peripheral nerves are known to be good substrates for bridging CNS trauma. The involvement of fibroblast growth factor-2 (FGF-2) activation in the dorsal root ganglion (DRG) was examined following spinal cord injury in the rat. We evaluated whether FGF-2 increases the ability of a sciatic nerve graft to enhance neuronal plasticity, in a gap promoted by complete transection of the spinal cord. The rats were subjected to a 4 mm-long gap at low thoracic level and were repaired with saline (Saline or control group, n = 10), or fragment of the sciatic nerve (Nerve group, n = 10), or fragment of the sciatic nerve to which FGF-2 (Nerve + FGF-2 group, n = 10) had been added immediately after lesion. The effects of the FGF-2 and fragment of the sciatic nerve grafts on neuronal plasticity were investigated using choline acetyl transferase (ChAT)-immunoreactivity of neurons in the dorsal root ganglion after 8 weeks. Preservation of the area and diameter of neuronal cell bodies in dorsal root ganglion (DRG) was seen in animals treated with the sciatic nerve, an effect enhanced by the addition of FGF-2. Thus, the addition of exogenous FGF-2 to a sciatic nerve fragment grafted in a gap of the rat spinal cord submitted to complete transection was able to improve neuroprotection in the DRG. The results emphasized that the manipulation of the microenvironment in the wound might amplify the regenerative capacity of peripheral neurons |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-03 |
dc.date.accessioned.fl_str_mv |
2021-03-05T11:06:24Z |
dc.date.available.fl_str_mv |
2021-03-05T11:06:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
GUZEN, Fausto Pierdoná; ARAÚJO, Dayane Pessoa de; LUCENA, Eudes Euler de Souza; MORAIS, Hécio Henrique Araújo de; CAVALCANTI, José Rodolfo Lopes de Paiva; NASCIMENTO JUNIOR, Expedito Silva do; COSTA, Miriam Stela Maris de Oliveira; CAVALCANTE, Jeferson de Souza. Effect of FGF-2 and sciatic nerve grafting on ChAT expression in dorsal root ganglia neurons of spinal cord transected rats. Neuroscience Letters, [s. l.], v. 616, p. 43-48, mar. 2016. Elsevier BV. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S0304394015301130?via%3Dihub#!. Acesso em: 23 jul. 2020. http://dx.doi.org/10.1016/j.neulet.2015.08.043 |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/handle/123456789/31695 |
dc.identifier.issn.none.fl_str_mv |
0304-3940 (print) |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.neulet.2015.08.043 |
identifier_str_mv |
GUZEN, Fausto Pierdoná; ARAÚJO, Dayane Pessoa de; LUCENA, Eudes Euler de Souza; MORAIS, Hécio Henrique Araújo de; CAVALCANTI, José Rodolfo Lopes de Paiva; NASCIMENTO JUNIOR, Expedito Silva do; COSTA, Miriam Stela Maris de Oliveira; CAVALCANTE, Jeferson de Souza. Effect of FGF-2 and sciatic nerve grafting on ChAT expression in dorsal root ganglia neurons of spinal cord transected rats. Neuroscience Letters, [s. l.], v. 616, p. 43-48, mar. 2016. Elsevier BV. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S0304394015301130?via%3Dihub#!. Acesso em: 23 jul. 2020. http://dx.doi.org/10.1016/j.neulet.2015.08.043 0304-3940 (print) 10.1016/j.neulet.2015.08.043 |
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https://repositorio.ufrn.br/handle/123456789/31695 |
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eng |
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Attribution 3.0 Brazil http://creativecommons.org/licenses/by/3.0/br/ info:eu-repo/semantics/openAccess |
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Attribution 3.0 Brazil http://creativecommons.org/licenses/by/3.0/br/ |
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openAccess |
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Elsevier |
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Elsevier |
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