Effect of FGF-2 and sciatic nerve grafting on ChAT expression in dorsal root ganglia neurons of spinal cord transected rats

Detalhes bibliográficos
Autor(a) principal: Guzen, Fausto Pierdoná
Data de Publicação: 2016
Outros Autores: Araújo, Dayane Pessoa de, Lucena, Eudes Euler de Souza, Morais, Hécio Henrique Araújo de, Cavalcanti, José Rodolfo Lopes de Paiva, Nascimento Junior, Expedito Silva do, Costa, Miriam Stela Maris de Oliveira, Cavalcante, Jeferson de Souza
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/handle/123456789/31695
Resumo: Neurotrophic factors and peripheral nerves are known to be good substrates for bridging CNS trauma. The involvement of fibroblast growth factor-2 (FGF-2) activation in the dorsal root ganglion (DRG) was examined following spinal cord injury in the rat. We evaluated whether FGF-2 increases the ability of a sciatic nerve graft to enhance neuronal plasticity, in a gap promoted by complete transection of the spinal cord. The rats were subjected to a 4 mm-long gap at low thoracic level and were repaired with saline (Saline or control group, n = 10), or fragment of the sciatic nerve (Nerve group, n = 10), or fragment of the sciatic nerve to which FGF-2 (Nerve + FGF-2 group, n = 10) had been added immediately after lesion. The effects of the FGF-2 and fragment of the sciatic nerve grafts on neuronal plasticity were investigated using choline acetyl transferase (ChAT)-immunoreactivity of neurons in the dorsal root ganglion after 8 weeks. Preservation of the area and diameter of neuronal cell bodies in dorsal root ganglion (DRG) was seen in animals treated with the sciatic nerve, an effect enhanced by the addition of FGF-2. Thus, the addition of exogenous FGF-2 to a sciatic nerve fragment grafted in a gap of the rat spinal cord submitted to complete transection was able to improve neuroprotection in the DRG. The results emphasized that the manipulation of the microenvironment in the wound might amplify the regenerative capacity of peripheral neurons
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spelling Guzen, Fausto PierdonáAraújo, Dayane Pessoa deLucena, Eudes Euler de SouzaMorais, Hécio Henrique Araújo deCavalcanti, José Rodolfo Lopes de PaivaNascimento Junior, Expedito Silva doCosta, Miriam Stela Maris de OliveiraCavalcante, Jeferson de Souza2021-03-05T11:06:24Z2021-03-05T11:06:24Z2016-03GUZEN, Fausto Pierdoná; ARAÚJO, Dayane Pessoa de; LUCENA, Eudes Euler de Souza; MORAIS, Hécio Henrique Araújo de; CAVALCANTI, José Rodolfo Lopes de Paiva; NASCIMENTO JUNIOR, Expedito Silva do; COSTA, Miriam Stela Maris de Oliveira; CAVALCANTE, Jeferson de Souza. Effect of FGF-2 and sciatic nerve grafting on ChAT expression in dorsal root ganglia neurons of spinal cord transected rats. Neuroscience Letters, [s. l.], v. 616, p. 43-48, mar. 2016. Elsevier BV. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S0304394015301130?via%3Dihub#!. Acesso em: 23 jul. 2020. http://dx.doi.org/10.1016/j.neulet.2015.08.0430304-3940 (print)https://repositorio.ufrn.br/handle/123456789/3169510.1016/j.neulet.2015.08.043ElsevierAttribution 3.0 Brazilhttp://creativecommons.org/licenses/by/3.0/br/info:eu-repo/semantics/openAccessDorsal root gangliaFibroblastic growth factor-2NeuroprotectionSciatic nerve graftSpinal cordEffect of FGF-2 and sciatic nerve grafting on ChAT expression in dorsal root ganglia neurons of spinal cord transected ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleNeurotrophic factors and peripheral nerves are known to be good substrates for bridging CNS trauma. The involvement of fibroblast growth factor-2 (FGF-2) activation in the dorsal root ganglion (DRG) was examined following spinal cord injury in the rat. We evaluated whether FGF-2 increases the ability of a sciatic nerve graft to enhance neuronal plasticity, in a gap promoted by complete transection of the spinal cord. The rats were subjected to a 4 mm-long gap at low thoracic level and were repaired with saline (Saline or control group, n = 10), or fragment of the sciatic nerve (Nerve group, n = 10), or fragment of the sciatic nerve to which FGF-2 (Nerve + FGF-2 group, n = 10) had been added immediately after lesion. The effects of the FGF-2 and fragment of the sciatic nerve grafts on neuronal plasticity were investigated using choline acetyl transferase (ChAT)-immunoreactivity of neurons in the dorsal root ganglion after 8 weeks. Preservation of the area and diameter of neuronal cell bodies in dorsal root ganglion (DRG) was seen in animals treated with the sciatic nerve, an effect enhanced by the addition of FGF-2. Thus, the addition of exogenous FGF-2 to a sciatic nerve fragment grafted in a gap of the rat spinal cord submitted to complete transection was able to improve neuroprotection in the DRG. 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dc.title.pt_BR.fl_str_mv Effect of FGF-2 and sciatic nerve grafting on ChAT expression in dorsal root ganglia neurons of spinal cord transected rats
title Effect of FGF-2 and sciatic nerve grafting on ChAT expression in dorsal root ganglia neurons of spinal cord transected rats
spellingShingle Effect of FGF-2 and sciatic nerve grafting on ChAT expression in dorsal root ganglia neurons of spinal cord transected rats
Guzen, Fausto Pierdoná
Dorsal root ganglia
Fibroblastic growth factor-2
Neuroprotection
Sciatic nerve graft
Spinal cord
title_short Effect of FGF-2 and sciatic nerve grafting on ChAT expression in dorsal root ganglia neurons of spinal cord transected rats
title_full Effect of FGF-2 and sciatic nerve grafting on ChAT expression in dorsal root ganglia neurons of spinal cord transected rats
title_fullStr Effect of FGF-2 and sciatic nerve grafting on ChAT expression in dorsal root ganglia neurons of spinal cord transected rats
title_full_unstemmed Effect of FGF-2 and sciatic nerve grafting on ChAT expression in dorsal root ganglia neurons of spinal cord transected rats
title_sort Effect of FGF-2 and sciatic nerve grafting on ChAT expression in dorsal root ganglia neurons of spinal cord transected rats
author Guzen, Fausto Pierdoná
author_facet Guzen, Fausto Pierdoná
Araújo, Dayane Pessoa de
Lucena, Eudes Euler de Souza
Morais, Hécio Henrique Araújo de
Cavalcanti, José Rodolfo Lopes de Paiva
Nascimento Junior, Expedito Silva do
Costa, Miriam Stela Maris de Oliveira
Cavalcante, Jeferson de Souza
author_role author
author2 Araújo, Dayane Pessoa de
Lucena, Eudes Euler de Souza
Morais, Hécio Henrique Araújo de
Cavalcanti, José Rodolfo Lopes de Paiva
Nascimento Junior, Expedito Silva do
Costa, Miriam Stela Maris de Oliveira
Cavalcante, Jeferson de Souza
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Guzen, Fausto Pierdoná
Araújo, Dayane Pessoa de
Lucena, Eudes Euler de Souza
Morais, Hécio Henrique Araújo de
Cavalcanti, José Rodolfo Lopes de Paiva
Nascimento Junior, Expedito Silva do
Costa, Miriam Stela Maris de Oliveira
Cavalcante, Jeferson de Souza
dc.subject.por.fl_str_mv Dorsal root ganglia
Fibroblastic growth factor-2
Neuroprotection
Sciatic nerve graft
Spinal cord
topic Dorsal root ganglia
Fibroblastic growth factor-2
Neuroprotection
Sciatic nerve graft
Spinal cord
description Neurotrophic factors and peripheral nerves are known to be good substrates for bridging CNS trauma. The involvement of fibroblast growth factor-2 (FGF-2) activation in the dorsal root ganglion (DRG) was examined following spinal cord injury in the rat. We evaluated whether FGF-2 increases the ability of a sciatic nerve graft to enhance neuronal plasticity, in a gap promoted by complete transection of the spinal cord. The rats were subjected to a 4 mm-long gap at low thoracic level and were repaired with saline (Saline or control group, n = 10), or fragment of the sciatic nerve (Nerve group, n = 10), or fragment of the sciatic nerve to which FGF-2 (Nerve + FGF-2 group, n = 10) had been added immediately after lesion. The effects of the FGF-2 and fragment of the sciatic nerve grafts on neuronal plasticity were investigated using choline acetyl transferase (ChAT)-immunoreactivity of neurons in the dorsal root ganglion after 8 weeks. Preservation of the area and diameter of neuronal cell bodies in dorsal root ganglion (DRG) was seen in animals treated with the sciatic nerve, an effect enhanced by the addition of FGF-2. Thus, the addition of exogenous FGF-2 to a sciatic nerve fragment grafted in a gap of the rat spinal cord submitted to complete transection was able to improve neuroprotection in the DRG. The results emphasized that the manipulation of the microenvironment in the wound might amplify the regenerative capacity of peripheral neurons
publishDate 2016
dc.date.issued.fl_str_mv 2016-03
dc.date.accessioned.fl_str_mv 2021-03-05T11:06:24Z
dc.date.available.fl_str_mv 2021-03-05T11:06:24Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.citation.fl_str_mv GUZEN, Fausto Pierdoná; ARAÚJO, Dayane Pessoa de; LUCENA, Eudes Euler de Souza; MORAIS, Hécio Henrique Araújo de; CAVALCANTI, José Rodolfo Lopes de Paiva; NASCIMENTO JUNIOR, Expedito Silva do; COSTA, Miriam Stela Maris de Oliveira; CAVALCANTE, Jeferson de Souza. Effect of FGF-2 and sciatic nerve grafting on ChAT expression in dorsal root ganglia neurons of spinal cord transected rats. Neuroscience Letters, [s. l.], v. 616, p. 43-48, mar. 2016. Elsevier BV. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S0304394015301130?via%3Dihub#!. Acesso em: 23 jul. 2020. http://dx.doi.org/10.1016/j.neulet.2015.08.043
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/handle/123456789/31695
dc.identifier.issn.none.fl_str_mv 0304-3940 (print)
dc.identifier.doi.none.fl_str_mv 10.1016/j.neulet.2015.08.043
identifier_str_mv GUZEN, Fausto Pierdoná; ARAÚJO, Dayane Pessoa de; LUCENA, Eudes Euler de Souza; MORAIS, Hécio Henrique Araújo de; CAVALCANTI, José Rodolfo Lopes de Paiva; NASCIMENTO JUNIOR, Expedito Silva do; COSTA, Miriam Stela Maris de Oliveira; CAVALCANTE, Jeferson de Souza. Effect of FGF-2 and sciatic nerve grafting on ChAT expression in dorsal root ganglia neurons of spinal cord transected rats. Neuroscience Letters, [s. l.], v. 616, p. 43-48, mar. 2016. Elsevier BV. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S0304394015301130?via%3Dihub#!. Acesso em: 23 jul. 2020. http://dx.doi.org/10.1016/j.neulet.2015.08.043
0304-3940 (print)
10.1016/j.neulet.2015.08.043
url https://repositorio.ufrn.br/handle/123456789/31695
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