Chromosome 17 abnormalities and mutation of the TP53 gene: Correlation between cytogenetics, flow cytometry and molecular analysis in three cases of chronic myeloid leukemia

Detalhes bibliográficos
Autor(a) principal: Otero, Luize
Data de Publicação: 2005
Outros Autores: Cavalcanti Júnior, Geraldo Barroso, Klumb, Claudete Esteves, Scheiner, Marcos Antonio Mauricio, Magluta, Eliane Pereira Simões, Fernandez, Teresa de Souza, Silva, Maria Luiza Macedo, Pires, Virgínia, Andrade, Gabriela Vasconcelos, Maia, Raquel Ciuvalschi, Tabak, Daniel
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/handle/123456789/40854
Resumo: Alterations involving the short arm of chromosome 17 (17p) during the progression of chronic myeloid leukemia (CML) have been described. This chromosomal region contains the tumor suppressor gene TP53 that may be an important factor in the evolution of this disease. In this study, we used flow cytometry and western blotting to assess p53 protein expression and single stranded conformational polymorphism to examine TP53 gene alterations in three patients with CML who showed alterations in 17p. Only the case with del(17)(p11) had p53 expression positive by flow cytometry and an abnormal migration pattern by SSCP analysis. The importance of the correlation between the results obtained with these techniques, as well as the clinical course of the patients, are discussed.
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spelling Otero, LuizeCavalcanti Júnior, Geraldo BarrosoKlumb, Claudete EstevesScheiner, Marcos Antonio MauricioMagluta, Eliane Pereira SimõesFernandez, Teresa de SouzaSilva, Maria Luiza MacedoPires, VirgíniaAndrade, Gabriela VasconcelosMaia, Raquel CiuvalschiTabak, Daniel2021-09-30T19:15:36Z2021-09-30T19:15:36Z2005OTERO, Luize; CAVALCANTI JÚNIOR, Geraldo Barroso; KLUMB, Claudete Esteves; SCHEINER, Marcos Antonio Mauricio; MAGLUTA, Eliane Pereira Simões; FERNANDEZ, Teresa de Souza; SILVA, Maria Luiza Macedo; PIRES, Virgínia; ANDRADE, Gabriela Vasconcelos; MAIA, Raquel Ciuvalschi; TABAK, Daniel. Chromosome 17 abnormalities and mutation of the TP53 gene: Correlation between cytogenetics, flow cytometry and molecular analysis in three cases of chronic myeloid leukemia. Genetics and Molecular Biology, p. 40-43, 2005. Disponível em: https://www.scielo.br/j/gmb/i/2005.v28n1/ Acesso em:30 set. 2021.1415-4757https://repositorio.ufrn.br/handle/123456789/40854Sociedade Brasileira de GenéticaChronic myeloid leukemiaChromosome 17TP53 geneP53 proteinChromosome 17 abnormalities and mutation of the TP53 gene: Correlation between cytogenetics, flow cytometry and molecular analysis in three cases of chronic myeloid leukemiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAlterations involving the short arm of chromosome 17 (17p) during the progression of chronic myeloid leukemia (CML) have been described. This chromosomal region contains the tumor suppressor gene TP53 that may be an important factor in the evolution of this disease. In this study, we used flow cytometry and western blotting to assess p53 protein expression and single stranded conformational polymorphism to examine TP53 gene alterations in three patients with CML who showed alterations in 17p. Only the case with del(17)(p11) had p53 expression positive by flow cytometry and an abnormal migration pattern by SSCP analysis. The importance of the correlation between the results obtained with these techniques, as well as the clinical course of the patients, are discussed.porreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNinfo:eu-repo/semantics/openAccessORIGINALChromosomeAbnormalitiesMutation_CalvalcantiJunior_2005.pdfChromosomeAbnormalitiesMutation_CalvalcantiJunior_2005.pdfapplication/pdf177436https://repositorio.ufrn.br/bitstream/123456789/40854/1/ChromosomeAbnormalitiesMutation_CalvalcantiJunior_2005.pdff62287077331abf6fa58e4bae4950a03MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81597https://repositorio.ufrn.br/bitstream/123456789/40854/2/license.txtc06df47c0e9e9a9e1042ec6b4e4f68b5MD52123456789/408542021-09-30 16:15:37.455oai:https://repositorio.ufrn.br:123456789/40854TElDRU7Dh0HCoERFIERJU1RSSUJVScOHw4NPIE7Dg08tRVhDTFVTSVZBDQoNCg0KQW8gYXNzaW5hciBlIGVudHJlZ2FyIGVzdGHCoGxpY2Vuw6dhLCBvL2EgU3IuL1NyYS4gKGF1dG9yIG91IGRldGVudG9yIGRvcyBkaXJlaXRvcyBkZSBhdXRvcik6DQoNCg0KYSkgQ29uY2VkZSDDoCBVbml2ZXJzaWRhZGUgRmVkZXJhbCBkbyBSaW8gR3JhbmRlIGRvIE5vcnRlIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZQ0KcmVwcm9kdXppciwgY29udmVydGVyIChjb21vIGRlZmluaWRvIGFiYWl4byksIGNvbXVuaWNhciBlL291DQpkaXN0cmlidWlyIG8gZG9jdW1lbnRvIGVudHJlZ3VlIChpbmNsdWluZG8gbyByZXN1bW8vYWJzdHJhY3QpIGVtDQpmb3JtYXRvIGRpZ2l0YWwgb3UgaW1wcmVzc28gZSBlbSBxdWFscXVlciBtZWlvLg0KDQpiKSBEZWNsYXJhIHF1ZSBvIGRvY3VtZW50byBlbnRyZWd1ZSDDqSBzZXUgdHJhYmFsaG8gb3JpZ2luYWwsIGUgcXVlDQpkZXTDqW0gbyBkaXJlaXRvIGRlIGNvbmNlZGVyIG9zIGRpcmVpdG9zIGNvbnRpZG9zIG5lc3RhwqBsaWNlbsOnYS4gRGVjbGFyYQ0KdGFtYsOpbSBxdWUgYSBlbnRyZWdhIGRvIGRvY3VtZW50byBuw6NvIGluZnJpbmdlLCB0YW50byBxdWFudG8gbGhlIMOpDQpwb3Nzw612ZWwgc2FiZXIsIG9zIGRpcmVpdG9zIGRlIHF1YWxxdWVyIG91dHJhIHBlc3NvYSBvdSBlbnRpZGFkZS4NCg0KYykgU2UgbyBkb2N1bWVudG8gZW50cmVndWUgY29udMOpbSBtYXRlcmlhbCBkbyBxdWFsIG7Do28gZGV0w6ltIG9zDQpkaXJlaXRvcyBkZSBhdXRvciwgZGVjbGFyYSBxdWUgb2J0ZXZlIGF1dG9yaXphw6fDo28gZG8gZGV0ZW50b3IgZG9zDQpkaXJlaXRvcyBkZSBhdXRvciBwYXJhIGNvbmNlZGVyIMOgIFVuaXZlcnNpZGFkZSBGZWRlcmFsIGRvIFJpbyBHcmFuZGUgZG8gTm9ydGUgb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YcKgbGljZW7Dp2EsIGUgcXVlIGVzc2UgbWF0ZXJpYWwgY3Vqb3MgZGlyZWl0b3Mgc8OjbyBkZQ0KdGVyY2Vpcm9zIGVzdMOhIGNsYXJhbWVudGUgaWRlbnRpZmljYWRvIGUgcmVjb25oZWNpZG8gbm8gdGV4dG8gb3UNCmNvbnRlw7pkbyBkbyBkb2N1bWVudG8gZW50cmVndWUuDQoNClNlIG8gZG9jdW1lbnRvIGVudHJlZ3VlIMOpIGJhc2VhZG8gZW0gdHJhYmFsaG8gZmluYW5jaWFkbyBvdSBhcG9pYWRvDQpwb3Igb3V0cmEgaW5zdGl0dWnDp8OjbyBxdWUgbsOjbyBhIFVuaXZlcnNpZGFkZSBGZWRlcmFsIGRvIFJpbyBHcmFuZGUgZG8gTm9ydGUsIGRlY2xhcmEgcXVlIGN1bXByaXUgcXVhaXNxdWVyIG9icmlnYcOnw7VlcyBleGlnaWRhcyBwZWxvIHJlc3BlY3Rpdm8gY29udHJhdG8gb3UgYWNvcmRvLg0KDQpBIFVuaXZlcnNpZGFkZSBGZWRlcmFsIGRvIFJpbyBHcmFuZGUgZG8gTm9ydGUgaWRlbnRpZmljYXLDoSBjbGFyYW1lbnRlIG8ocykgc2V1IChzKSBub21lKHMpIGNvbW8gbyAocykgYXV0b3IgKGVzKSBvdSBkZXRlbnRvciAoZXMpIGRvcyBkaXJlaXRvcyBkbyBkb2N1bWVudG8NCmVudHJlZ3VlLCBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIHBhcmEgYWzDqW0gZGFzIHBlcm1pdGlkYXMgcG9yDQplc3RhwqBsaWNlbsOnYS4NCg==Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2021-09-30T19:15:37Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.pt_BR.fl_str_mv Chromosome 17 abnormalities and mutation of the TP53 gene: Correlation between cytogenetics, flow cytometry and molecular analysis in three cases of chronic myeloid leukemia
title Chromosome 17 abnormalities and mutation of the TP53 gene: Correlation between cytogenetics, flow cytometry and molecular analysis in three cases of chronic myeloid leukemia
spellingShingle Chromosome 17 abnormalities and mutation of the TP53 gene: Correlation between cytogenetics, flow cytometry and molecular analysis in three cases of chronic myeloid leukemia
Otero, Luize
Chronic myeloid leukemia
Chromosome 17
TP53 gene
P53 protein
title_short Chromosome 17 abnormalities and mutation of the TP53 gene: Correlation between cytogenetics, flow cytometry and molecular analysis in three cases of chronic myeloid leukemia
title_full Chromosome 17 abnormalities and mutation of the TP53 gene: Correlation between cytogenetics, flow cytometry and molecular analysis in three cases of chronic myeloid leukemia
title_fullStr Chromosome 17 abnormalities and mutation of the TP53 gene: Correlation between cytogenetics, flow cytometry and molecular analysis in three cases of chronic myeloid leukemia
title_full_unstemmed Chromosome 17 abnormalities and mutation of the TP53 gene: Correlation between cytogenetics, flow cytometry and molecular analysis in three cases of chronic myeloid leukemia
title_sort Chromosome 17 abnormalities and mutation of the TP53 gene: Correlation between cytogenetics, flow cytometry and molecular analysis in three cases of chronic myeloid leukemia
author Otero, Luize
author_facet Otero, Luize
Cavalcanti Júnior, Geraldo Barroso
Klumb, Claudete Esteves
Scheiner, Marcos Antonio Mauricio
Magluta, Eliane Pereira Simões
Fernandez, Teresa de Souza
Silva, Maria Luiza Macedo
Pires, Virgínia
Andrade, Gabriela Vasconcelos
Maia, Raquel Ciuvalschi
Tabak, Daniel
author_role author
author2 Cavalcanti Júnior, Geraldo Barroso
Klumb, Claudete Esteves
Scheiner, Marcos Antonio Mauricio
Magluta, Eliane Pereira Simões
Fernandez, Teresa de Souza
Silva, Maria Luiza Macedo
Pires, Virgínia
Andrade, Gabriela Vasconcelos
Maia, Raquel Ciuvalschi
Tabak, Daniel
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Otero, Luize
Cavalcanti Júnior, Geraldo Barroso
Klumb, Claudete Esteves
Scheiner, Marcos Antonio Mauricio
Magluta, Eliane Pereira Simões
Fernandez, Teresa de Souza
Silva, Maria Luiza Macedo
Pires, Virgínia
Andrade, Gabriela Vasconcelos
Maia, Raquel Ciuvalschi
Tabak, Daniel
dc.subject.por.fl_str_mv Chronic myeloid leukemia
Chromosome 17
TP53 gene
P53 protein
topic Chronic myeloid leukemia
Chromosome 17
TP53 gene
P53 protein
description Alterations involving the short arm of chromosome 17 (17p) during the progression of chronic myeloid leukemia (CML) have been described. This chromosomal region contains the tumor suppressor gene TP53 that may be an important factor in the evolution of this disease. In this study, we used flow cytometry and western blotting to assess p53 protein expression and single stranded conformational polymorphism to examine TP53 gene alterations in three patients with CML who showed alterations in 17p. Only the case with del(17)(p11) had p53 expression positive by flow cytometry and an abnormal migration pattern by SSCP analysis. The importance of the correlation between the results obtained with these techniques, as well as the clinical course of the patients, are discussed.
publishDate 2005
dc.date.issued.fl_str_mv 2005
dc.date.accessioned.fl_str_mv 2021-09-30T19:15:36Z
dc.date.available.fl_str_mv 2021-09-30T19:15:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv OTERO, Luize; CAVALCANTI JÚNIOR, Geraldo Barroso; KLUMB, Claudete Esteves; SCHEINER, Marcos Antonio Mauricio; MAGLUTA, Eliane Pereira Simões; FERNANDEZ, Teresa de Souza; SILVA, Maria Luiza Macedo; PIRES, Virgínia; ANDRADE, Gabriela Vasconcelos; MAIA, Raquel Ciuvalschi; TABAK, Daniel. Chromosome 17 abnormalities and mutation of the TP53 gene: Correlation between cytogenetics, flow cytometry and molecular analysis in three cases of chronic myeloid leukemia. Genetics and Molecular Biology, p. 40-43, 2005. Disponível em: https://www.scielo.br/j/gmb/i/2005.v28n1/ Acesso em:30 set. 2021.
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/handle/123456789/40854
dc.identifier.issn.none.fl_str_mv 1415-4757
identifier_str_mv OTERO, Luize; CAVALCANTI JÚNIOR, Geraldo Barroso; KLUMB, Claudete Esteves; SCHEINER, Marcos Antonio Mauricio; MAGLUTA, Eliane Pereira Simões; FERNANDEZ, Teresa de Souza; SILVA, Maria Luiza Macedo; PIRES, Virgínia; ANDRADE, Gabriela Vasconcelos; MAIA, Raquel Ciuvalschi; TABAK, Daniel. Chromosome 17 abnormalities and mutation of the TP53 gene: Correlation between cytogenetics, flow cytometry and molecular analysis in three cases of chronic myeloid leukemia. Genetics and Molecular Biology, p. 40-43, 2005. Disponível em: https://www.scielo.br/j/gmb/i/2005.v28n1/ Acesso em:30 set. 2021.
1415-4757
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dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFRN
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