Ocorrência de enterobactérias isoladas de baratas Periplaneta americana
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Tipo de documento: | Trabalho de conclusão de curso |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/handle/123456789/47316 |
Resumo: | Cockroaches may be found in almost all kind of habitats. Most of them are wild, however a small group of those insects live in urban areas and may come in contact with people. They are vector of many micro-organisms which may cause deseases in humans. The objective of this study is to verify the occurrence of enterobacteria on the cuticle of Periplaneta americana in both male and female catched in Centro de Biociências in Universidade Federal do Rio Grande do Norte, investigate if there is resistent bacteria regarding tested antibiotics and analise diferences between male and female of cockroaches when it comes down to the occurrence of enterobacteria. In all, twenty cockroaches were collected in the fat boxes and then taken to the Laboratory of Medical Microbiology, where the bacteria present in the cuticle of those insects. The bacteria were sown on MacConkey agar and subjected to Gram test, biochemical tests and antibiogram. 42 bacteria were isolates and Escherichia coli represented more than 80% of strains and were present in both males and females of P. americana. However, two species of bacteria appeared only in males and only two in females. The antibiogram showed bacteria resistant to Imipenem, an important broad-spectrum antibiotic. It is possible to find bacteria on the cuticle in both sexes of Periplaneta americana, including enterobacteria resistant to carbapenem. Further studies regarding the mechanism of resistance of these bacteria are important to understand whether these bacteria have developed an efficient resistance mechanism against that antibiotic. |
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Alcântara, Dhierich de SousaMelo, Maria Celeste Nunes deMelo, Maria Celeste Nunes deFernandes, Maria José de Britto CostaAlmeida, Elineí Araújo deAndreazze, Ricardo2017-12-07T13:38:27Z2022-05-25T11:36:23Z2017-12-07T13:38:27Z2022-05-25T11:36:23Z2017-11-162011043037ALCÂNTARA, Dhierich de Sousa. Ocorrência de enterobactérias isoladas de baratas periplaneta americana. 2017. 20 f. Monografia (Graduação em Ciências Biológicas) - Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal-RN, 2017.https://repositorio.ufrn.br/handle/123456789/47316Cockroaches may be found in almost all kind of habitats. Most of them are wild, however a small group of those insects live in urban areas and may come in contact with people. They are vector of many micro-organisms which may cause deseases in humans. The objective of this study is to verify the occurrence of enterobacteria on the cuticle of Periplaneta americana in both male and female catched in Centro de Biociências in Universidade Federal do Rio Grande do Norte, investigate if there is resistent bacteria regarding tested antibiotics and analise diferences between male and female of cockroaches when it comes down to the occurrence of enterobacteria. In all, twenty cockroaches were collected in the fat boxes and then taken to the Laboratory of Medical Microbiology, where the bacteria present in the cuticle of those insects. The bacteria were sown on MacConkey agar and subjected to Gram test, biochemical tests and antibiogram. 42 bacteria were isolates and Escherichia coli represented more than 80% of strains and were present in both males and females of P. americana. However, two species of bacteria appeared only in males and only two in females. The antibiogram showed bacteria resistant to Imipenem, an important broad-spectrum antibiotic. It is possible to find bacteria on the cuticle in both sexes of Periplaneta americana, including enterobacteria resistant to carbapenem. Further studies regarding the mechanism of resistance of these bacteria are important to understand whether these bacteria have developed an efficient resistance mechanism against that antibiotic.As baratas podem ser encontradas em quase todos os habitats. A maioria são silvestres, mas um pequeno grupo desses insetos habitam zonas urbanas e podem entrar em contato com as pessoas. Elas são vetores de diversos micro-organismos que podem causar doenças em seres humanos. O objetivo deste estudo foi verificar a ocorrência de enterobactérias presentes no exoesqueleto de machos e fêmeas de baratas da espécie Periplaneta americana coletadas no Centro de Biociências da Universidade Federal do Rio Grande do Norte, bem como investigar se há resistência importante dessas bactérias em relação aos antibióticos testados e verificar se há diferenças nos machos e nas fêmeas desses insetos quanto a ocorrência das enterobactérias transportadas. Ao todo, vinte baratas foram coletadas nas caixas de gordura e depois levadas ao Laboratório de Microbiologia Médica, onde foram isoladas as bactérias presentes no exoesqueleto dos insetos. As bactérias foram semeadas em ágar MacConkey e submetidas a coloração de Gram, testes bioquímicos e antibiograma. De 42 cepas isoladas, a espécie Escherichia coli representou mais de 80% das cepas e estavam presentes tanto em machos quanto em fêmeas de P. americana. No entanto, duas espécies de bactérias apareceram apenas em machos e duas apenas em fêmeas. O antibiograma apresentou bactérias resistentes ao Imipenem, um importante antibiótico de amplo espectro. É possível encontrar bactérias nos dois sexos de Periplaneta americana, inclusive enterobactérias resistentes a carbapenem. É importante um estudo adicional acerca do mecanismo de resistência dessas bactérias para compreender se essas bactérias desenvolveram um mecanismo de resistência eficiente contra esse antibiótico.Universidade Federal do Rio Grande do NorteUFRNBrasilCiências BiológicasEnterobactériasEnterobacteriaAntibióticosAntibioticsBaratasCockrochesCNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::MICROBIOLOGIA MEDICAOcorrência de enterobactérias isoladas de baratas Periplaneta americanainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/openAccessporreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNTEXTOcorrenciaEnterobacterias_Alcantara_2017.pdf.txtExtracted texttext/plain31595https://repositorio.ufrn.br/bitstream/123456789/47316/1/OcorrenciaEnterobacterias_Alcantara_2017.pdf.txt3850cf86921f3ea7f5203f977a454cddMD51ORIGINALOcorrenciaEnterobacterias_Alcantara_2017.pdfMonografiaapplication/pdf1182374https://repositorio.ufrn.br/bitstream/123456789/47316/2/OcorrenciaEnterobacterias_Alcantara_2017.pdf0cfbad08a0be67e3fc3faa1371b2a924MD52LICENSElicense.txttext/plain756https://repositorio.ufrn.br/bitstream/123456789/47316/3/license.txta80a9cda2756d355b388cc443c3d8a43MD53123456789/473162022-05-25 08:36:23.223oai:https://repositorio.ufrn.br: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ório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2022-05-25T11:36:23Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pr_BR.fl_str_mv |
Ocorrência de enterobactérias isoladas de baratas Periplaneta americana |
title |
Ocorrência de enterobactérias isoladas de baratas Periplaneta americana |
spellingShingle |
Ocorrência de enterobactérias isoladas de baratas Periplaneta americana Alcântara, Dhierich de Sousa Enterobactérias Enterobacteria Antibióticos Antibiotics Baratas Cockroches CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::MICROBIOLOGIA MEDICA |
title_short |
Ocorrência de enterobactérias isoladas de baratas Periplaneta americana |
title_full |
Ocorrência de enterobactérias isoladas de baratas Periplaneta americana |
title_fullStr |
Ocorrência de enterobactérias isoladas de baratas Periplaneta americana |
title_full_unstemmed |
Ocorrência de enterobactérias isoladas de baratas Periplaneta americana |
title_sort |
Ocorrência de enterobactérias isoladas de baratas Periplaneta americana |
author |
Alcântara, Dhierich de Sousa |
author_facet |
Alcântara, Dhierich de Sousa |
author_role |
author |
dc.contributor.referees1.none.fl_str_mv |
Melo, Maria Celeste Nunes de |
dc.contributor.referees2.none.fl_str_mv |
Fernandes, Maria José de Britto Costa |
dc.contributor.referees3.none.fl_str_mv |
Almeida, Elineí Araújo de |
dc.contributor.author.fl_str_mv |
Alcântara, Dhierich de Sousa |
dc.contributor.advisor-co1.fl_str_mv |
Melo, Maria Celeste Nunes de |
dc.contributor.advisor1.fl_str_mv |
Andreazze, Ricardo |
contributor_str_mv |
Melo, Maria Celeste Nunes de Andreazze, Ricardo |
dc.subject.pr_BR.fl_str_mv |
Enterobactérias Enterobacteria Antibióticos Antibiotics Baratas Cockroches |
topic |
Enterobactérias Enterobacteria Antibióticos Antibiotics Baratas Cockroches CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::MICROBIOLOGIA MEDICA |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::MICROBIOLOGIA MEDICA |
description |
Cockroaches may be found in almost all kind of habitats. Most of them are wild, however a small group of those insects live in urban areas and may come in contact with people. They are vector of many micro-organisms which may cause deseases in humans. The objective of this study is to verify the occurrence of enterobacteria on the cuticle of Periplaneta americana in both male and female catched in Centro de Biociências in Universidade Federal do Rio Grande do Norte, investigate if there is resistent bacteria regarding tested antibiotics and analise diferences between male and female of cockroaches when it comes down to the occurrence of enterobacteria. In all, twenty cockroaches were collected in the fat boxes and then taken to the Laboratory of Medical Microbiology, where the bacteria present in the cuticle of those insects. The bacteria were sown on MacConkey agar and subjected to Gram test, biochemical tests and antibiogram. 42 bacteria were isolates and Escherichia coli represented more than 80% of strains and were present in both males and females of P. americana. However, two species of bacteria appeared only in males and only two in females. The antibiogram showed bacteria resistant to Imipenem, an important broad-spectrum antibiotic. It is possible to find bacteria on the cuticle in both sexes of Periplaneta americana, including enterobacteria resistant to carbapenem. Further studies regarding the mechanism of resistance of these bacteria are important to understand whether these bacteria have developed an efficient resistance mechanism against that antibiotic. |
publishDate |
2017 |
dc.date.accessioned.fl_str_mv |
2017-12-07T13:38:27Z 2022-05-25T11:36:23Z |
dc.date.available.fl_str_mv |
2017-12-07T13:38:27Z 2022-05-25T11:36:23Z |
dc.date.issued.fl_str_mv |
2017-11-16 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/bachelorThesis |
format |
bachelorThesis |
status_str |
publishedVersion |
dc.identifier.pr_BR.fl_str_mv |
2011043037 |
dc.identifier.citation.fl_str_mv |
ALCÂNTARA, Dhierich de Sousa. Ocorrência de enterobactérias isoladas de baratas periplaneta americana. 2017. 20 f. Monografia (Graduação em Ciências Biológicas) - Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal-RN, 2017. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/handle/123456789/47316 |
identifier_str_mv |
2011043037 ALCÂNTARA, Dhierich de Sousa. Ocorrência de enterobactérias isoladas de baratas periplaneta americana. 2017. 20 f. Monografia (Graduação em Ciências Biológicas) - Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal-RN, 2017. |
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https://repositorio.ufrn.br/handle/123456789/47316 |
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por |
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Universidade Federal do Rio Grande do Norte |
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UFRN |
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Brasil |
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Ciências Biológicas |
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Universidade Federal do Rio Grande do Norte |
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