Ocorrência de enterobactérias isoladas de baratas Periplaneta americana

Detalhes bibliográficos
Autor(a) principal: Alcântara, Dhierich de Sousa
Data de Publicação: 2017
Tipo de documento: Trabalho de conclusão de curso
Idioma: por
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/handle/123456789/47316
Resumo: Cockroaches may be found in almost all kind of habitats. Most of them are wild, however a small group of those insects live in urban areas and may come in contact with people. They are vector of many micro-organisms which may cause deseases in humans. The objective of this study is to verify the occurrence of enterobacteria on the cuticle of Periplaneta americana in both male and female catched in Centro de Biociências in Universidade Federal do Rio Grande do Norte, investigate if there is resistent bacteria regarding tested antibiotics and analise diferences between male and female of cockroaches when it comes down to the occurrence of enterobacteria. In all, twenty cockroaches were collected in the fat boxes and then taken to the Laboratory of Medical Microbiology, where the bacteria present in the cuticle of those insects. The bacteria were sown on MacConkey agar and subjected to Gram test, biochemical tests and antibiogram. 42 bacteria were isolates and Escherichia coli represented more than 80% of strains and were present in both males and females of P. americana. However, two species of bacteria appeared only in males and only two in females. The antibiogram showed bacteria resistant to Imipenem, an important broad-spectrum antibiotic. It is possible to find bacteria on the cuticle in both sexes of Periplaneta americana, including enterobacteria resistant to carbapenem. Further studies regarding the mechanism of resistance of these bacteria are important to understand whether these bacteria have developed an efficient resistance mechanism against that antibiotic.
id UFRN_b34775c498a3815b555c441c6839082f
oai_identifier_str oai:https://repositorio.ufrn.br:123456789/47316
network_acronym_str UFRN
network_name_str Repositório Institucional da UFRN
repository_id_str
spelling Alcântara, Dhierich de SousaMelo, Maria Celeste Nunes deMelo, Maria Celeste Nunes deFernandes, Maria José de Britto CostaAlmeida, Elineí Araújo deAndreazze, Ricardo2017-12-07T13:38:27Z2022-05-25T11:36:23Z2017-12-07T13:38:27Z2022-05-25T11:36:23Z2017-11-162011043037ALCÂNTARA, Dhierich de Sousa. Ocorrência de enterobactérias isoladas de baratas periplaneta americana. 2017. 20 f. Monografia (Graduação em Ciências Biológicas) - Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal-RN, 2017.https://repositorio.ufrn.br/handle/123456789/47316Cockroaches may be found in almost all kind of habitats. Most of them are wild, however a small group of those insects live in urban areas and may come in contact with people. They are vector of many micro-organisms which may cause deseases in humans. The objective of this study is to verify the occurrence of enterobacteria on the cuticle of Periplaneta americana in both male and female catched in Centro de Biociências in Universidade Federal do Rio Grande do Norte, investigate if there is resistent bacteria regarding tested antibiotics and analise diferences between male and female of cockroaches when it comes down to the occurrence of enterobacteria. In all, twenty cockroaches were collected in the fat boxes and then taken to the Laboratory of Medical Microbiology, where the bacteria present in the cuticle of those insects. The bacteria were sown on MacConkey agar and subjected to Gram test, biochemical tests and antibiogram. 42 bacteria were isolates and Escherichia coli represented more than 80% of strains and were present in both males and females of P. americana. However, two species of bacteria appeared only in males and only two in females. The antibiogram showed bacteria resistant to Imipenem, an important broad-spectrum antibiotic. It is possible to find bacteria on the cuticle in both sexes of Periplaneta americana, including enterobacteria resistant to carbapenem. Further studies regarding the mechanism of resistance of these bacteria are important to understand whether these bacteria have developed an efficient resistance mechanism against that antibiotic.As baratas podem ser encontradas em quase todos os habitats. A maioria são silvestres, mas um pequeno grupo desses insetos habitam zonas urbanas e podem entrar em contato com as pessoas. Elas são vetores de diversos micro-organismos que podem causar doenças em seres humanos. O objetivo deste estudo foi verificar a ocorrência de enterobactérias presentes no exoesqueleto de machos e fêmeas de baratas da espécie Periplaneta americana coletadas no Centro de Biociências da Universidade Federal do Rio Grande do Norte, bem como investigar se há resistência importante dessas bactérias em relação aos antibióticos testados e verificar se há diferenças nos machos e nas fêmeas desses insetos quanto a ocorrência das enterobactérias transportadas. Ao todo, vinte baratas foram coletadas nas caixas de gordura e depois levadas ao Laboratório de Microbiologia Médica, onde foram isoladas as bactérias presentes no exoesqueleto dos insetos. As bactérias foram semeadas em ágar MacConkey e submetidas a coloração de Gram, testes bioquímicos e antibiograma. De 42 cepas isoladas, a espécie Escherichia coli representou mais de 80% das cepas e estavam presentes tanto em machos quanto em fêmeas de P. americana. No entanto, duas espécies de bactérias apareceram apenas em machos e duas apenas em fêmeas. O antibiograma apresentou bactérias resistentes ao Imipenem, um importante antibiótico de amplo espectro. É possível encontrar bactérias nos dois sexos de Periplaneta americana, inclusive enterobactérias resistentes a carbapenem. É importante um estudo adicional acerca do mecanismo de resistência dessas bactérias para compreender se essas bactérias desenvolveram um mecanismo de resistência eficiente contra esse antibiótico.Universidade Federal do Rio Grande do NorteUFRNBrasilCiências BiológicasEnterobactériasEnterobacteriaAntibióticosAntibioticsBaratasCockrochesCNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::MICROBIOLOGIA MEDICAOcorrência de enterobactérias isoladas de baratas Periplaneta americanainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/openAccessporreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNTEXTOcorrenciaEnterobacterias_Alcantara_2017.pdf.txtExtracted texttext/plain31595https://repositorio.ufrn.br/bitstream/123456789/47316/1/OcorrenciaEnterobacterias_Alcantara_2017.pdf.txt3850cf86921f3ea7f5203f977a454cddMD51ORIGINALOcorrenciaEnterobacterias_Alcantara_2017.pdfMonografiaapplication/pdf1182374https://repositorio.ufrn.br/bitstream/123456789/47316/2/OcorrenciaEnterobacterias_Alcantara_2017.pdf0cfbad08a0be67e3fc3faa1371b2a924MD52LICENSElicense.txttext/plain756https://repositorio.ufrn.br/bitstream/123456789/47316/3/license.txta80a9cda2756d355b388cc443c3d8a43MD53123456789/473162022-05-25 08:36:23.223oai:https://repositorio.ufrn.br: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ório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2022-05-25T11:36:23Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.pr_BR.fl_str_mv Ocorrência de enterobactérias isoladas de baratas Periplaneta americana
title Ocorrência de enterobactérias isoladas de baratas Periplaneta americana
spellingShingle Ocorrência de enterobactérias isoladas de baratas Periplaneta americana
Alcântara, Dhierich de Sousa
Enterobactérias
Enterobacteria
Antibióticos
Antibiotics
Baratas
Cockroches
CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::MICROBIOLOGIA MEDICA
title_short Ocorrência de enterobactérias isoladas de baratas Periplaneta americana
title_full Ocorrência de enterobactérias isoladas de baratas Periplaneta americana
title_fullStr Ocorrência de enterobactérias isoladas de baratas Periplaneta americana
title_full_unstemmed Ocorrência de enterobactérias isoladas de baratas Periplaneta americana
title_sort Ocorrência de enterobactérias isoladas de baratas Periplaneta americana
author Alcântara, Dhierich de Sousa
author_facet Alcântara, Dhierich de Sousa
author_role author
dc.contributor.referees1.none.fl_str_mv Melo, Maria Celeste Nunes de
dc.contributor.referees2.none.fl_str_mv Fernandes, Maria José de Britto Costa
dc.contributor.referees3.none.fl_str_mv Almeida, Elineí Araújo de
dc.contributor.author.fl_str_mv Alcântara, Dhierich de Sousa
dc.contributor.advisor-co1.fl_str_mv Melo, Maria Celeste Nunes de
dc.contributor.advisor1.fl_str_mv Andreazze, Ricardo
contributor_str_mv Melo, Maria Celeste Nunes de
Andreazze, Ricardo
dc.subject.pr_BR.fl_str_mv Enterobactérias
Enterobacteria
Antibióticos
Antibiotics
Baratas
Cockroches
topic Enterobactérias
Enterobacteria
Antibióticos
Antibiotics
Baratas
Cockroches
CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::MICROBIOLOGIA MEDICA
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::MICROBIOLOGIA MEDICA
description Cockroaches may be found in almost all kind of habitats. Most of them are wild, however a small group of those insects live in urban areas and may come in contact with people. They are vector of many micro-organisms which may cause deseases in humans. The objective of this study is to verify the occurrence of enterobacteria on the cuticle of Periplaneta americana in both male and female catched in Centro de Biociências in Universidade Federal do Rio Grande do Norte, investigate if there is resistent bacteria regarding tested antibiotics and analise diferences between male and female of cockroaches when it comes down to the occurrence of enterobacteria. In all, twenty cockroaches were collected in the fat boxes and then taken to the Laboratory of Medical Microbiology, where the bacteria present in the cuticle of those insects. The bacteria were sown on MacConkey agar and subjected to Gram test, biochemical tests and antibiogram. 42 bacteria were isolates and Escherichia coli represented more than 80% of strains and were present in both males and females of P. americana. However, two species of bacteria appeared only in males and only two in females. The antibiogram showed bacteria resistant to Imipenem, an important broad-spectrum antibiotic. It is possible to find bacteria on the cuticle in both sexes of Periplaneta americana, including enterobacteria resistant to carbapenem. Further studies regarding the mechanism of resistance of these bacteria are important to understand whether these bacteria have developed an efficient resistance mechanism against that antibiotic.
publishDate 2017
dc.date.accessioned.fl_str_mv 2017-12-07T13:38:27Z
2022-05-25T11:36:23Z
dc.date.available.fl_str_mv 2017-12-07T13:38:27Z
2022-05-25T11:36:23Z
dc.date.issued.fl_str_mv 2017-11-16
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/bachelorThesis
format bachelorThesis
status_str publishedVersion
dc.identifier.pr_BR.fl_str_mv 2011043037
dc.identifier.citation.fl_str_mv ALCÂNTARA, Dhierich de Sousa. Ocorrência de enterobactérias isoladas de baratas periplaneta americana. 2017. 20 f. Monografia (Graduação em Ciências Biológicas) - Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal-RN, 2017.
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/handle/123456789/47316
identifier_str_mv 2011043037
ALCÂNTARA, Dhierich de Sousa. Ocorrência de enterobactérias isoladas de baratas periplaneta americana. 2017. 20 f. Monografia (Graduação em Ciências Biológicas) - Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal-RN, 2017.
url https://repositorio.ufrn.br/handle/123456789/47316
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal do Rio Grande do Norte
dc.publisher.initials.fl_str_mv UFRN
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Ciências Biológicas
publisher.none.fl_str_mv Universidade Federal do Rio Grande do Norte
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFRN
instname:Universidade Federal do Rio Grande do Norte (UFRN)
instacron:UFRN
instname_str Universidade Federal do Rio Grande do Norte (UFRN)
instacron_str UFRN
institution UFRN
reponame_str Repositório Institucional da UFRN
collection Repositório Institucional da UFRN
bitstream.url.fl_str_mv https://repositorio.ufrn.br/bitstream/123456789/47316/1/OcorrenciaEnterobacterias_Alcantara_2017.pdf.txt
https://repositorio.ufrn.br/bitstream/123456789/47316/2/OcorrenciaEnterobacterias_Alcantara_2017.pdf
https://repositorio.ufrn.br/bitstream/123456789/47316/3/license.txt
bitstream.checksum.fl_str_mv 3850cf86921f3ea7f5203f977a454cdd
0cfbad08a0be67e3fc3faa1371b2a924
a80a9cda2756d355b388cc443c3d8a43
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)
repository.mail.fl_str_mv
_version_ 1797777224212938752