Advanced caries microbiota in teeth with irreversible pulpitis
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/jspui/handle/123456789/22914 |
Resumo: | INTRODUCTION: Bacterial taxa in the forefront of caries biofilms are candidate pathogens for irreversible pulpitis and are possibly the first ones to invade the pulp and initiate endodontic infection. This study examined the microbiota of the most advanced layers of dentinal caries in teeth with irreversible pulpitis. METHODS: DNA extracted from samples taken from deep dentinal caries associated with pulp exposures was analyzed for the presence and relative levels of 33 oral bacterial taxa by using reverse-capture checkerboard hybridization assay. Quantification of total bacteria, streptococci, and lactobacilli was also performed by using real-time quantitative polymerase chain reaction. Associations between the target bacterial taxa and clinical signs/symptoms were also evaluated. RESULTS: The most frequently detected taxa in the checkerboard assay were Atopobium genomospecies C1 (53%), Pseudoramibacter alactolyticus (37%), Streptococcus species (33%), Streptococcus mutans (33%), Parvimonas micra (13%), Fusobacterium nucleatum (13%), and Veillonella species (13%). Streptococcus species, Dialister invisus, and P. micra were significantly associated with throbbing pain, S. mutans with pain to percussion, and Lactobacillus with continuous pain (P < .05). Quantitative polymerase chain reaction revealed a mean total bacterial load of 1 × 10(8) (range, 2.05 × 10(5) to 4.5 × 10(8)) cell equivalents per milligram (wet weight) of dentin. Streptococci and lactobacilli were very prevalent but comprised only 0.09% and 2% of the whole bacterial population, respectively. CONCLUSIONS: Several bacterial taxa were found in advanced caries lesions in teeth with exposed pulps, and some of them were significantly associated with symptoms. A role for these taxa in the etiology of irreversible pulpitis is suspected. |
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Rôças, Isabela N.Lima, Kenio Costa deAssunção, Isauremi Vieira deGomes, Patrícia N.Bracks, Igor V.Siqueira, José F.2017-05-16T12:03:46Z2017-05-16T12:03:46Z2015RÔÇAS, Isabela N. et al.. Advanced caries microbiota in teeth with irreversible pulpitis. Journal of Endodontics, v. 41, n. 9, p. 1450-55, 2015.https://repositorio.ufrn.br/jspui/handle/123456789/22914engDentinal cariesLactobacillusStreptococcusIrreversible pulpitisMicrobiotaMolecular biologyPermanent teethAdvanced caries microbiota in teeth with irreversible pulpitisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleINTRODUCTION: Bacterial taxa in the forefront of caries biofilms are candidate pathogens for irreversible pulpitis and are possibly the first ones to invade the pulp and initiate endodontic infection. This study examined the microbiota of the most advanced layers of dentinal caries in teeth with irreversible pulpitis. METHODS: DNA extracted from samples taken from deep dentinal caries associated with pulp exposures was analyzed for the presence and relative levels of 33 oral bacterial taxa by using reverse-capture checkerboard hybridization assay. Quantification of total bacteria, streptococci, and lactobacilli was also performed by using real-time quantitative polymerase chain reaction. Associations between the target bacterial taxa and clinical signs/symptoms were also evaluated. RESULTS: The most frequently detected taxa in the checkerboard assay were Atopobium genomospecies C1 (53%), Pseudoramibacter alactolyticus (37%), Streptococcus species (33%), Streptococcus mutans (33%), Parvimonas micra (13%), Fusobacterium nucleatum (13%), and Veillonella species (13%). Streptococcus species, Dialister invisus, and P. micra were significantly associated with throbbing pain, S. mutans with pain to percussion, and Lactobacillus with continuous pain (P < .05). Quantitative polymerase chain reaction revealed a mean total bacterial load of 1 × 10(8) (range, 2.05 × 10(5) to 4.5 × 10(8)) cell equivalents per milligram (wet weight) of dentin. Streptococci and lactobacilli were very prevalent but comprised only 0.09% and 2% of the whole bacterial population, respectively. CONCLUSIONS: Several bacterial taxa were found in advanced caries lesions in teeth with exposed pulps, and some of them were significantly associated with symptoms. A role for these taxa in the etiology of irreversible pulpitis is suspected.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNLICENSElicense.txtlicense.txttext/plain; charset=utf-81569https://repositorio.ufrn.br/bitstream/123456789/22914/2/license.txt6e6f57145bc87daf99079f06b081ff9fMD52ORIGINALAdvancedCariesMicrobiota_Rocas_2015.pdfAdvancedCariesMicrobiota_Rocas_2015.pdfhttps://www.ncbi.nlm.nih.gov/pubmed/26187422application/pdf432475https://repositorio.ufrn.br/bitstream/123456789/22914/1/AdvancedCariesMicrobiota_Rocas_2015.pdfad78a8f7b005e7adbd49cc2681958435MD51TEXTAdvanced Caries Microbiota_2015.pdf.txtAdvanced Caries Microbiota_2015.pdf.txtExtracted texttext/plain35397https://repositorio.ufrn.br/bitstream/123456789/22914/5/Advanced%20Caries%20Microbiota_2015.pdf.txtdd981f53c36401a34ebd271af5619439MD55THUMBNAILAdvanced Caries Microbiota_2015.pdf.jpgAdvanced Caries Microbiota_2015.pdf.jpgIM Thumbnailimage/jpeg9748https://repositorio.ufrn.br/bitstream/123456789/22914/6/Advanced%20Caries%20Microbiota_2015.pdf.jpgfb420d45526baa22af8f4afa25e2e373MD56123456789/229142021-12-20 13:49:03.011oai:https://repositorio.ufrn.br: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ório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2021-12-20T16:49:03Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
Advanced caries microbiota in teeth with irreversible pulpitis |
title |
Advanced caries microbiota in teeth with irreversible pulpitis |
spellingShingle |
Advanced caries microbiota in teeth with irreversible pulpitis Rôças, Isabela N. Dentinal caries Lactobacillus Streptococcus Irreversible pulpitis Microbiota Molecular biology Permanent teeth |
title_short |
Advanced caries microbiota in teeth with irreversible pulpitis |
title_full |
Advanced caries microbiota in teeth with irreversible pulpitis |
title_fullStr |
Advanced caries microbiota in teeth with irreversible pulpitis |
title_full_unstemmed |
Advanced caries microbiota in teeth with irreversible pulpitis |
title_sort |
Advanced caries microbiota in teeth with irreversible pulpitis |
author |
Rôças, Isabela N. |
author_facet |
Rôças, Isabela N. Lima, Kenio Costa de Assunção, Isauremi Vieira de Gomes, Patrícia N. Bracks, Igor V. Siqueira, José F. |
author_role |
author |
author2 |
Lima, Kenio Costa de Assunção, Isauremi Vieira de Gomes, Patrícia N. Bracks, Igor V. Siqueira, José F. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Rôças, Isabela N. Lima, Kenio Costa de Assunção, Isauremi Vieira de Gomes, Patrícia N. Bracks, Igor V. Siqueira, José F. |
dc.subject.por.fl_str_mv |
Dentinal caries Lactobacillus Streptococcus Irreversible pulpitis Microbiota Molecular biology Permanent teeth |
topic |
Dentinal caries Lactobacillus Streptococcus Irreversible pulpitis Microbiota Molecular biology Permanent teeth |
description |
INTRODUCTION: Bacterial taxa in the forefront of caries biofilms are candidate pathogens for irreversible pulpitis and are possibly the first ones to invade the pulp and initiate endodontic infection. This study examined the microbiota of the most advanced layers of dentinal caries in teeth with irreversible pulpitis. METHODS: DNA extracted from samples taken from deep dentinal caries associated with pulp exposures was analyzed for the presence and relative levels of 33 oral bacterial taxa by using reverse-capture checkerboard hybridization assay. Quantification of total bacteria, streptococci, and lactobacilli was also performed by using real-time quantitative polymerase chain reaction. Associations between the target bacterial taxa and clinical signs/symptoms were also evaluated. RESULTS: The most frequently detected taxa in the checkerboard assay were Atopobium genomospecies C1 (53%), Pseudoramibacter alactolyticus (37%), Streptococcus species (33%), Streptococcus mutans (33%), Parvimonas micra (13%), Fusobacterium nucleatum (13%), and Veillonella species (13%). Streptococcus species, Dialister invisus, and P. micra were significantly associated with throbbing pain, S. mutans with pain to percussion, and Lactobacillus with continuous pain (P < .05). Quantitative polymerase chain reaction revealed a mean total bacterial load of 1 × 10(8) (range, 2.05 × 10(5) to 4.5 × 10(8)) cell equivalents per milligram (wet weight) of dentin. Streptococci and lactobacilli were very prevalent but comprised only 0.09% and 2% of the whole bacterial population, respectively. CONCLUSIONS: Several bacterial taxa were found in advanced caries lesions in teeth with exposed pulps, and some of them were significantly associated with symptoms. A role for these taxa in the etiology of irreversible pulpitis is suspected. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015 |
dc.date.accessioned.fl_str_mv |
2017-05-16T12:03:46Z |
dc.date.available.fl_str_mv |
2017-05-16T12:03:46Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
RÔÇAS, Isabela N. et al.. Advanced caries microbiota in teeth with irreversible pulpitis. Journal of Endodontics, v. 41, n. 9, p. 1450-55, 2015. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/jspui/handle/123456789/22914 |
identifier_str_mv |
RÔÇAS, Isabela N. et al.. Advanced caries microbiota in teeth with irreversible pulpitis. Journal of Endodontics, v. 41, n. 9, p. 1450-55, 2015. |
url |
https://repositorio.ufrn.br/jspui/handle/123456789/22914 |
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eng |
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