Specific phospholipids regulate the acquisition of neuronal and astroglial identities in post-mitotic cells

Detalhes bibliográficos
Autor(a) principal: Montaner, Aneley
Data de Publicação: 2018
Outros Autores: Santana, Themis Taynah da Silva, Schroeder, Timm, Einiker-Lamas, Marcelo, Girardini, Javier, Costa, Marcos Romualdo, Banchio, Claudia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/jspui/handle/123456789/24537
Resumo: Hitherto, the known mechanisms underpinning cell-fate specification act on neural progenitors, affecting their commitment to generate neuron or glial cells. Here, we show that particular phospholipids supplemented in the culture media modify the commitment of post-mitotic neural cells in vitro. Phosphatidylcholine (PtdCho)-enriched media enhances neuronal differentiation at the expense of astroglial and unspecified cells. Conversely, phosphatidylethanolamine (PtdEtn) enhances astroglial differentiation and accelerates astrocyte maturation. The ability of phospholipids to modify the fate of post-mitotic cells depends on its presence during a narrow time-window during cell differentiation and it is mediated by the selective activation of particular signaling pathways. While PtdCho-mediated effect on neuronal differentiation depends on cAMP-dependent kinase (PKA)/calcium responsive element binding protein (CREB), PtdEtn stimulates astrogliogenesis through the activation of the MEK/ERK signaling pathway. Collectively, our results provide an additional degree of plasticity in neural cell specification and further support the notion that cell differentiation is a reversible phenomenon. They also contribute to our understanding of neuronal and glial lineage specification in the central nervous system, opening up new avenues to retrieve neurogenic capacity in the brain.
id UFRN_bddc581c27a8712f83b49dffdfe52a1b
oai_identifier_str oai:https://repositorio.ufrn.br:123456789/24537
network_acronym_str UFRN
network_name_str Repositório Institucional da UFRN
repository_id_str
spelling Montaner, AneleySantana, Themis Taynah da SilvaSchroeder, TimmEiniker-Lamas, MarceloGirardini, JavierCosta, Marcos RomualdoBanchio, Claudia2018-01-12T11:48:21Z2018-01-12T11:48:21Z2018-01-11https://repositorio.ufrn.br/jspui/handle/123456789/2453710.1038/s41598-017-18700-4engglial cellspost-mitotic cellsphospholipidscell plasticitycell biologySpecific phospholipids regulate the acquisition of neuronal and astroglial identities in post-mitotic cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleHitherto, the known mechanisms underpinning cell-fate specification act on neural progenitors, affecting their commitment to generate neuron or glial cells. Here, we show that particular phospholipids supplemented in the culture media modify the commitment of post-mitotic neural cells in vitro. Phosphatidylcholine (PtdCho)-enriched media enhances neuronal differentiation at the expense of astroglial and unspecified cells. Conversely, phosphatidylethanolamine (PtdEtn) enhances astroglial differentiation and accelerates astrocyte maturation. The ability of phospholipids to modify the fate of post-mitotic cells depends on its presence during a narrow time-window during cell differentiation and it is mediated by the selective activation of particular signaling pathways. While PtdCho-mediated effect on neuronal differentiation depends on cAMP-dependent kinase (PKA)/calcium responsive element binding protein (CREB), PtdEtn stimulates astrogliogenesis through the activation of the MEK/ERK signaling pathway. Collectively, our results provide an additional degree of plasticity in neural cell specification and further support the notion that cell differentiation is a reversible phenomenon. They also contribute to our understanding of neuronal and glial lineage specification in the central nervous system, opening up new avenues to retrieve neurogenic capacity in the brain.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALMarcosCosta_ICe_2018_Specific phospholipids regulate.pdfMarcosCosta_ICe_2018_Specific phospholipids regulate.pdfMarcosCosta_ICe_2018_Specific phospholipids regulateapplication/pdf4531572https://repositorio.ufrn.br/bitstream/123456789/24537/1/MarcosCosta_ICe_2018_Specific%20phospholipids%20regulate.pdf7397f225209eb6e8dccbc265ed311dcfMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.ufrn.br/bitstream/123456789/24537/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52TEXTMarcosCosta_ICe_2018_Specific phospholipids regulate.pdf.txtMarcosCosta_ICe_2018_Specific phospholipids regulate.pdf.txtExtracted texttext/plain61492https://repositorio.ufrn.br/bitstream/123456789/24537/3/MarcosCosta_ICe_2018_Specific%20phospholipids%20regulate.pdf.txt5979ae83483c574c6ab522301c97becbMD53THUMBNAILMarcosCosta_ICe_2018_Specific phospholipids regulate.pdf.jpgMarcosCosta_ICe_2018_Specific phospholipids regulate.pdf.jpgIM Thumbnailimage/jpeg9702https://repositorio.ufrn.br/bitstream/123456789/24537/4/MarcosCosta_ICe_2018_Specific%20phospholipids%20regulate.pdf.jpgff7f43130ccfdfd876b26dc5aa09c012MD54123456789/245372019-01-30 00:48:02.712oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2019-01-30T03:48:02Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.pt_BR.fl_str_mv Specific phospholipids regulate the acquisition of neuronal and astroglial identities in post-mitotic cells
title Specific phospholipids regulate the acquisition of neuronal and astroglial identities in post-mitotic cells
spellingShingle Specific phospholipids regulate the acquisition of neuronal and astroglial identities in post-mitotic cells
Montaner, Aneley
glial cells
post-mitotic cells
phospholipids
cell plasticity
cell biology
title_short Specific phospholipids regulate the acquisition of neuronal and astroglial identities in post-mitotic cells
title_full Specific phospholipids regulate the acquisition of neuronal and astroglial identities in post-mitotic cells
title_fullStr Specific phospholipids regulate the acquisition of neuronal and astroglial identities in post-mitotic cells
title_full_unstemmed Specific phospholipids regulate the acquisition of neuronal and astroglial identities in post-mitotic cells
title_sort Specific phospholipids regulate the acquisition of neuronal and astroglial identities in post-mitotic cells
author Montaner, Aneley
author_facet Montaner, Aneley
Santana, Themis Taynah da Silva
Schroeder, Timm
Einiker-Lamas, Marcelo
Girardini, Javier
Costa, Marcos Romualdo
Banchio, Claudia
author_role author
author2 Santana, Themis Taynah da Silva
Schroeder, Timm
Einiker-Lamas, Marcelo
Girardini, Javier
Costa, Marcos Romualdo
Banchio, Claudia
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Montaner, Aneley
Santana, Themis Taynah da Silva
Schroeder, Timm
Einiker-Lamas, Marcelo
Girardini, Javier
Costa, Marcos Romualdo
Banchio, Claudia
dc.subject.por.fl_str_mv glial cells
post-mitotic cells
phospholipids
cell plasticity
cell biology
topic glial cells
post-mitotic cells
phospholipids
cell plasticity
cell biology
description Hitherto, the known mechanisms underpinning cell-fate specification act on neural progenitors, affecting their commitment to generate neuron or glial cells. Here, we show that particular phospholipids supplemented in the culture media modify the commitment of post-mitotic neural cells in vitro. Phosphatidylcholine (PtdCho)-enriched media enhances neuronal differentiation at the expense of astroglial and unspecified cells. Conversely, phosphatidylethanolamine (PtdEtn) enhances astroglial differentiation and accelerates astrocyte maturation. The ability of phospholipids to modify the fate of post-mitotic cells depends on its presence during a narrow time-window during cell differentiation and it is mediated by the selective activation of particular signaling pathways. While PtdCho-mediated effect on neuronal differentiation depends on cAMP-dependent kinase (PKA)/calcium responsive element binding protein (CREB), PtdEtn stimulates astrogliogenesis through the activation of the MEK/ERK signaling pathway. Collectively, our results provide an additional degree of plasticity in neural cell specification and further support the notion that cell differentiation is a reversible phenomenon. They also contribute to our understanding of neuronal and glial lineage specification in the central nervous system, opening up new avenues to retrieve neurogenic capacity in the brain.
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-01-12T11:48:21Z
dc.date.available.fl_str_mv 2018-01-12T11:48:21Z
dc.date.issued.fl_str_mv 2018-01-11
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/jspui/handle/123456789/24537
dc.identifier.doi.none.fl_str_mv 10.1038/s41598-017-18700-4
url https://repositorio.ufrn.br/jspui/handle/123456789/24537
identifier_str_mv 10.1038/s41598-017-18700-4
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFRN
instname:Universidade Federal do Rio Grande do Norte (UFRN)
instacron:UFRN
instname_str Universidade Federal do Rio Grande do Norte (UFRN)
instacron_str UFRN
institution UFRN
reponame_str Repositório Institucional da UFRN
collection Repositório Institucional da UFRN
bitstream.url.fl_str_mv https://repositorio.ufrn.br/bitstream/123456789/24537/1/MarcosCosta_ICe_2018_Specific%20phospholipids%20regulate.pdf
https://repositorio.ufrn.br/bitstream/123456789/24537/2/license.txt
https://repositorio.ufrn.br/bitstream/123456789/24537/3/MarcosCosta_ICe_2018_Specific%20phospholipids%20regulate.pdf.txt
https://repositorio.ufrn.br/bitstream/123456789/24537/4/MarcosCosta_ICe_2018_Specific%20phospholipids%20regulate.pdf.jpg
bitstream.checksum.fl_str_mv 7397f225209eb6e8dccbc265ed311dcf
8a4605be74aa9ea9d79846c1fba20a33
5979ae83483c574c6ab522301c97becb
ff7f43130ccfdfd876b26dc5aa09c012
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)
repository.mail.fl_str_mv
_version_ 1814832943169798144