Specific phospholipids regulate the acquisition of neuronal and astroglial identities in post-mitotic cells
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/jspui/handle/123456789/24537 |
Resumo: | Hitherto, the known mechanisms underpinning cell-fate specification act on neural progenitors, affecting their commitment to generate neuron or glial cells. Here, we show that particular phospholipids supplemented in the culture media modify the commitment of post-mitotic neural cells in vitro. Phosphatidylcholine (PtdCho)-enriched media enhances neuronal differentiation at the expense of astroglial and unspecified cells. Conversely, phosphatidylethanolamine (PtdEtn) enhances astroglial differentiation and accelerates astrocyte maturation. The ability of phospholipids to modify the fate of post-mitotic cells depends on its presence during a narrow time-window during cell differentiation and it is mediated by the selective activation of particular signaling pathways. While PtdCho-mediated effect on neuronal differentiation depends on cAMP-dependent kinase (PKA)/calcium responsive element binding protein (CREB), PtdEtn stimulates astrogliogenesis through the activation of the MEK/ERK signaling pathway. Collectively, our results provide an additional degree of plasticity in neural cell specification and further support the notion that cell differentiation is a reversible phenomenon. They also contribute to our understanding of neuronal and glial lineage specification in the central nervous system, opening up new avenues to retrieve neurogenic capacity in the brain. |
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Montaner, AneleySantana, Themis Taynah da SilvaSchroeder, TimmEiniker-Lamas, MarceloGirardini, JavierCosta, Marcos RomualdoBanchio, Claudia2018-01-12T11:48:21Z2018-01-12T11:48:21Z2018-01-11https://repositorio.ufrn.br/jspui/handle/123456789/2453710.1038/s41598-017-18700-4engglial cellspost-mitotic cellsphospholipidscell plasticitycell biologySpecific phospholipids regulate the acquisition of neuronal and astroglial identities in post-mitotic cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleHitherto, the known mechanisms underpinning cell-fate specification act on neural progenitors, affecting their commitment to generate neuron or glial cells. Here, we show that particular phospholipids supplemented in the culture media modify the commitment of post-mitotic neural cells in vitro. Phosphatidylcholine (PtdCho)-enriched media enhances neuronal differentiation at the expense of astroglial and unspecified cells. Conversely, phosphatidylethanolamine (PtdEtn) enhances astroglial differentiation and accelerates astrocyte maturation. The ability of phospholipids to modify the fate of post-mitotic cells depends on its presence during a narrow time-window during cell differentiation and it is mediated by the selective activation of particular signaling pathways. While PtdCho-mediated effect on neuronal differentiation depends on cAMP-dependent kinase (PKA)/calcium responsive element binding protein (CREB), PtdEtn stimulates astrogliogenesis through the activation of the MEK/ERK signaling pathway. Collectively, our results provide an additional degree of plasticity in neural cell specification and further support the notion that cell differentiation is a reversible phenomenon. They also contribute to our understanding of neuronal and glial lineage specification in the central nervous system, opening up new avenues to retrieve neurogenic capacity in the brain.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALMarcosCosta_ICe_2018_Specific phospholipids regulate.pdfMarcosCosta_ICe_2018_Specific phospholipids regulate.pdfMarcosCosta_ICe_2018_Specific phospholipids regulateapplication/pdf4531572https://repositorio.ufrn.br/bitstream/123456789/24537/1/MarcosCosta_ICe_2018_Specific%20phospholipids%20regulate.pdf7397f225209eb6e8dccbc265ed311dcfMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.ufrn.br/bitstream/123456789/24537/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52TEXTMarcosCosta_ICe_2018_Specific phospholipids regulate.pdf.txtMarcosCosta_ICe_2018_Specific phospholipids regulate.pdf.txtExtracted texttext/plain61492https://repositorio.ufrn.br/bitstream/123456789/24537/3/MarcosCosta_ICe_2018_Specific%20phospholipids%20regulate.pdf.txt5979ae83483c574c6ab522301c97becbMD53THUMBNAILMarcosCosta_ICe_2018_Specific phospholipids regulate.pdf.jpgMarcosCosta_ICe_2018_Specific phospholipids regulate.pdf.jpgIM Thumbnailimage/jpeg9702https://repositorio.ufrn.br/bitstream/123456789/24537/4/MarcosCosta_ICe_2018_Specific%20phospholipids%20regulate.pdf.jpgff7f43130ccfdfd876b26dc5aa09c012MD54123456789/245372019-01-30 00:48:02.712oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2019-01-30T03:48:02Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
Specific phospholipids regulate the acquisition of neuronal and astroglial identities in post-mitotic cells |
title |
Specific phospholipids regulate the acquisition of neuronal and astroglial identities in post-mitotic cells |
spellingShingle |
Specific phospholipids regulate the acquisition of neuronal and astroglial identities in post-mitotic cells Montaner, Aneley glial cells post-mitotic cells phospholipids cell plasticity cell biology |
title_short |
Specific phospholipids regulate the acquisition of neuronal and astroglial identities in post-mitotic cells |
title_full |
Specific phospholipids regulate the acquisition of neuronal and astroglial identities in post-mitotic cells |
title_fullStr |
Specific phospholipids regulate the acquisition of neuronal and astroglial identities in post-mitotic cells |
title_full_unstemmed |
Specific phospholipids regulate the acquisition of neuronal and astroglial identities in post-mitotic cells |
title_sort |
Specific phospholipids regulate the acquisition of neuronal and astroglial identities in post-mitotic cells |
author |
Montaner, Aneley |
author_facet |
Montaner, Aneley Santana, Themis Taynah da Silva Schroeder, Timm Einiker-Lamas, Marcelo Girardini, Javier Costa, Marcos Romualdo Banchio, Claudia |
author_role |
author |
author2 |
Santana, Themis Taynah da Silva Schroeder, Timm Einiker-Lamas, Marcelo Girardini, Javier Costa, Marcos Romualdo Banchio, Claudia |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Montaner, Aneley Santana, Themis Taynah da Silva Schroeder, Timm Einiker-Lamas, Marcelo Girardini, Javier Costa, Marcos Romualdo Banchio, Claudia |
dc.subject.por.fl_str_mv |
glial cells post-mitotic cells phospholipids cell plasticity cell biology |
topic |
glial cells post-mitotic cells phospholipids cell plasticity cell biology |
description |
Hitherto, the known mechanisms underpinning cell-fate specification act on neural progenitors, affecting their commitment to generate neuron or glial cells. Here, we show that particular phospholipids supplemented in the culture media modify the commitment of post-mitotic neural cells in vitro. Phosphatidylcholine (PtdCho)-enriched media enhances neuronal differentiation at the expense of astroglial and unspecified cells. Conversely, phosphatidylethanolamine (PtdEtn) enhances astroglial differentiation and accelerates astrocyte maturation. The ability of phospholipids to modify the fate of post-mitotic cells depends on its presence during a narrow time-window during cell differentiation and it is mediated by the selective activation of particular signaling pathways. While PtdCho-mediated effect on neuronal differentiation depends on cAMP-dependent kinase (PKA)/calcium responsive element binding protein (CREB), PtdEtn stimulates astrogliogenesis through the activation of the MEK/ERK signaling pathway. Collectively, our results provide an additional degree of plasticity in neural cell specification and further support the notion that cell differentiation is a reversible phenomenon. They also contribute to our understanding of neuronal and glial lineage specification in the central nervous system, opening up new avenues to retrieve neurogenic capacity in the brain. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-01-12T11:48:21Z |
dc.date.available.fl_str_mv |
2018-01-12T11:48:21Z |
dc.date.issued.fl_str_mv |
2018-01-11 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/jspui/handle/123456789/24537 |
dc.identifier.doi.none.fl_str_mv |
10.1038/s41598-017-18700-4 |
url |
https://repositorio.ufrn.br/jspui/handle/123456789/24537 |
identifier_str_mv |
10.1038/s41598-017-18700-4 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFRN instname:Universidade Federal do Rio Grande do Norte (UFRN) instacron:UFRN |
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Universidade Federal do Rio Grande do Norte (UFRN) |
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UFRN |
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UFRN |
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Repositório Institucional da UFRN |
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