Enhancing enzymatic hydrolysis of green coconut fiber—Pretreatment assisted by tween 80 and water effect on the post-washing
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/handle/123456789/32628 |
Resumo: | This study evaluated the hydrothermal, alkaline (NaOH) and acid (H2SO4) pretreatment all combined with Tween 80 of the green coconut fiber aiming to improve the fermentable sugars production during the enzymatic hydrolysis. In addition, the water use during the post-washing of the pretreated materials was also assayed, seeking to ration its use without compromising the enzymatic hydrolysis and the removal of inhibitors. Untreated and pretreated biomass were assayed in terms of total solids, extractables, polysaccharides, lignin and total ashes according to the protocols proposed by NREL. Enzymatic hydrolysis was carried out using the preparation of cellulases from Trichoderma reesei ATCC 26921 and β-glucosidases (NS-22118 DCN00218) and xylanases (NS-22036 CDN01015). Additionally, the mass loss during pretreatment and washing was estimated. Results showed that the use of 3.0% (w/w) Tween 80 in the dilute alkaline pretreatment (2.0% (w/v) NaOH, 121 °C, 10 min) increased the conversion into glucose during the enzymatic hydrolysis of the pretreated materials from 48.7% to 56.1%. However, it did not show any influence on the pretreated materials in acid and hydrothermal conditions. The evaluation of the washing of the pretreated materials reduced the volume of water initially used in up to 75%, without affecting the final sugar production and the reduction of inhibitory compounds in the residual water, contributing to the viability of the production of cellulosic ethanol from green coconut fiber |
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Santos, Everaldo Silvino dosNogueira, Cleitiane da CostaPadilha, Carlos Eduardo de AraújoLeitão, Ana Laura de SáRocha, Patrícia MariaMacedo, Gorete Ribeiro de2021-06-10T13:59:40Z2021-06-10T13:59:40Z2018-02NOGUEIRA, C. C.; PADILHA, C. E.; LEITAO, A. L. S.; ROCHA, P. M.; MACEDO, G. R.; SANTOS, E. S.. Enhancing enzymatic hydrolysis of green coconut fiber-Pretreatment assisted by tween 80 and water effect on the post-washing. Industrial Crops and Products, v. 112, p. 734-740, 2018. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S0926669017308804?via%3Dihub. Acesso em: 20 abr. 2021. https://doi.org/10.1016/j.indcrop.2017.12.047.0926-6690https://repositorio.ufrn.br/handle/123456789/3262810.1016/j.indcrop.2017.12.047ElsevierAcidAlkalineHydrothermalSurfactantWashingCoconut fiberEnhancing enzymatic hydrolysis of green coconut fiber—Pretreatment assisted by tween 80 and water effect on the post-washinginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleThis study evaluated the hydrothermal, alkaline (NaOH) and acid (H2SO4) pretreatment all combined with Tween 80 of the green coconut fiber aiming to improve the fermentable sugars production during the enzymatic hydrolysis. In addition, the water use during the post-washing of the pretreated materials was also assayed, seeking to ration its use without compromising the enzymatic hydrolysis and the removal of inhibitors. Untreated and pretreated biomass were assayed in terms of total solids, extractables, polysaccharides, lignin and total ashes according to the protocols proposed by NREL. Enzymatic hydrolysis was carried out using the preparation of cellulases from Trichoderma reesei ATCC 26921 and β-glucosidases (NS-22118 DCN00218) and xylanases (NS-22036 CDN01015). Additionally, the mass loss during pretreatment and washing was estimated. Results showed that the use of 3.0% (w/w) Tween 80 in the dilute alkaline pretreatment (2.0% (w/v) NaOH, 121 °C, 10 min) increased the conversion into glucose during the enzymatic hydrolysis of the pretreated materials from 48.7% to 56.1%. However, it did not show any influence on the pretreated materials in acid and hydrothermal conditions. The evaluation of the washing of the pretreated materials reduced the volume of water initially used in up to 75%, without affecting the final sugar production and the reduction of inhibitory compounds in the residual water, contributing to the viability of the production of cellulosic ethanol from green coconut fiberengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNinfo:eu-repo/semantics/openAccessCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.ufrn.br/bitstream/123456789/32628/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/32628/3/license.txte9597aa2854d128fd968be5edc8a28d9MD53123456789/326282023-02-07 16:25:46.53oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2023-02-07T19:25:46Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
Enhancing enzymatic hydrolysis of green coconut fiber—Pretreatment assisted by tween 80 and water effect on the post-washing |
title |
Enhancing enzymatic hydrolysis of green coconut fiber—Pretreatment assisted by tween 80 and water effect on the post-washing |
spellingShingle |
Enhancing enzymatic hydrolysis of green coconut fiber—Pretreatment assisted by tween 80 and water effect on the post-washing Santos, Everaldo Silvino dos Acid Alkaline Hydrothermal Surfactant Washing Coconut fiber |
title_short |
Enhancing enzymatic hydrolysis of green coconut fiber—Pretreatment assisted by tween 80 and water effect on the post-washing |
title_full |
Enhancing enzymatic hydrolysis of green coconut fiber—Pretreatment assisted by tween 80 and water effect on the post-washing |
title_fullStr |
Enhancing enzymatic hydrolysis of green coconut fiber—Pretreatment assisted by tween 80 and water effect on the post-washing |
title_full_unstemmed |
Enhancing enzymatic hydrolysis of green coconut fiber—Pretreatment assisted by tween 80 and water effect on the post-washing |
title_sort |
Enhancing enzymatic hydrolysis of green coconut fiber—Pretreatment assisted by tween 80 and water effect on the post-washing |
author |
Santos, Everaldo Silvino dos |
author_facet |
Santos, Everaldo Silvino dos Nogueira, Cleitiane da Costa Padilha, Carlos Eduardo de Araújo Leitão, Ana Laura de Sá Rocha, Patrícia Maria Macedo, Gorete Ribeiro de |
author_role |
author |
author2 |
Nogueira, Cleitiane da Costa Padilha, Carlos Eduardo de Araújo Leitão, Ana Laura de Sá Rocha, Patrícia Maria Macedo, Gorete Ribeiro de |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Santos, Everaldo Silvino dos Nogueira, Cleitiane da Costa Padilha, Carlos Eduardo de Araújo Leitão, Ana Laura de Sá Rocha, Patrícia Maria Macedo, Gorete Ribeiro de |
dc.subject.por.fl_str_mv |
Acid Alkaline Hydrothermal Surfactant Washing Coconut fiber |
topic |
Acid Alkaline Hydrothermal Surfactant Washing Coconut fiber |
description |
This study evaluated the hydrothermal, alkaline (NaOH) and acid (H2SO4) pretreatment all combined with Tween 80 of the green coconut fiber aiming to improve the fermentable sugars production during the enzymatic hydrolysis. In addition, the water use during the post-washing of the pretreated materials was also assayed, seeking to ration its use without compromising the enzymatic hydrolysis and the removal of inhibitors. Untreated and pretreated biomass were assayed in terms of total solids, extractables, polysaccharides, lignin and total ashes according to the protocols proposed by NREL. Enzymatic hydrolysis was carried out using the preparation of cellulases from Trichoderma reesei ATCC 26921 and β-glucosidases (NS-22118 DCN00218) and xylanases (NS-22036 CDN01015). Additionally, the mass loss during pretreatment and washing was estimated. Results showed that the use of 3.0% (w/w) Tween 80 in the dilute alkaline pretreatment (2.0% (w/v) NaOH, 121 °C, 10 min) increased the conversion into glucose during the enzymatic hydrolysis of the pretreated materials from 48.7% to 56.1%. However, it did not show any influence on the pretreated materials in acid and hydrothermal conditions. The evaluation of the washing of the pretreated materials reduced the volume of water initially used in up to 75%, without affecting the final sugar production and the reduction of inhibitory compounds in the residual water, contributing to the viability of the production of cellulosic ethanol from green coconut fiber |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018-02 |
dc.date.accessioned.fl_str_mv |
2021-06-10T13:59:40Z |
dc.date.available.fl_str_mv |
2021-06-10T13:59:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
NOGUEIRA, C. C.; PADILHA, C. E.; LEITAO, A. L. S.; ROCHA, P. M.; MACEDO, G. R.; SANTOS, E. S.. Enhancing enzymatic hydrolysis of green coconut fiber-Pretreatment assisted by tween 80 and water effect on the post-washing. Industrial Crops and Products, v. 112, p. 734-740, 2018. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S0926669017308804?via%3Dihub. Acesso em: 20 abr. 2021. https://doi.org/10.1016/j.indcrop.2017.12.047. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/handle/123456789/32628 |
dc.identifier.issn.none.fl_str_mv |
0926-6690 |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.indcrop.2017.12.047 |
identifier_str_mv |
NOGUEIRA, C. C.; PADILHA, C. E.; LEITAO, A. L. S.; ROCHA, P. M.; MACEDO, G. R.; SANTOS, E. S.. Enhancing enzymatic hydrolysis of green coconut fiber-Pretreatment assisted by tween 80 and water effect on the post-washing. Industrial Crops and Products, v. 112, p. 734-740, 2018. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S0926669017308804?via%3Dihub. Acesso em: 20 abr. 2021. https://doi.org/10.1016/j.indcrop.2017.12.047. 0926-6690 10.1016/j.indcrop.2017.12.047 |
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https://repositorio.ufrn.br/handle/123456789/32628 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Elsevier |
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Elsevier |
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