Metagenômica comparativa de solo de regiões de mata atlântica e caatinga do estado do Rio Grande do Norte - Brasil
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/jspui/handle/123456789/12572 |
Resumo: | The microorganisms play very important roles in maintaining ecosystems, which explains the enormous interest in understanding the relationship between these organisms as well as between them and the environment. It is estimated that the total number of prokaryotic cells on Earth is between 4 and 6 x 1030, constituting an enormous biological and genetic pool to be explored. Although currently only 1% of all this wealth can be cultivated by standard laboratory techniques, metagenomic tools allow access to the genomic potential of environmental samples in a independent culture manner, and in combination with third generation sequencing technologies, the samples coverage become even greater. Soils, in particular, are the major reservoirs of this diversity, and many important environments around us, as the Brazilian biomes Caatinga and Atlantic Forest, are poorly studied. Thus, the genetic material from environmental soil samples of Caatinga and Atlantic Forest biomes were extracted by direct techniques, pyrosequenced, and the sequences generated were analyzed by bioinformatics programs (MEGAN MG-RAST and WEBCarma). Taxonomic comparative profiles of the samples showed that the phyla Proteobacteria, Actinobacteria, Acidobacteria and Planctomycetes were the most representative. In addition, fungi of the phylum Ascomycota were identified predominantly in the soil sample from the Atlantic Forest. Metabolic profiles showed that despite the existence of environmental differences, sequences from both samples were similarly placed in the various functional subsystems, indicating no specific habitat functions. This work, a pioneer in taxonomic and metabolic comparative analysis of soil samples from Brazilian biomes, contributes to the knowledge of these complex environmental systems, so far little explored |
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Pacchioni, Ralfo Goeshttp://lattes.cnpq.br/3944746730129166http://lattes.cnpq.br/1083882171718362Araújo, Magnólia Fernandes Florêncio dehttp://lattes.cnpq.br/4511989641459455Melo, Vânia Maria Macielhttp://lattes.cnpq.br/1572504650930605Lima, Lucymara Fassarela Agnez2014-12-17T14:03:36Z2011-05-122014-12-17T14:03:36Z2010-12-02PACCHIONI, Ralfo Goes. Metagenômica comparativa de solo de regiões de mata atlântica e caatinga do estado do Rio Grande do Norte - Brasil. 2010. 156 f. Dissertação (Mestrado em Bioquímica; Biologia Molecular) - Universidade Federal do Rio Grande do Norte, Natal, 2010.https://repositorio.ufrn.br/jspui/handle/123456789/12572The microorganisms play very important roles in maintaining ecosystems, which explains the enormous interest in understanding the relationship between these organisms as well as between them and the environment. It is estimated that the total number of prokaryotic cells on Earth is between 4 and 6 x 1030, constituting an enormous biological and genetic pool to be explored. Although currently only 1% of all this wealth can be cultivated by standard laboratory techniques, metagenomic tools allow access to the genomic potential of environmental samples in a independent culture manner, and in combination with third generation sequencing technologies, the samples coverage become even greater. Soils, in particular, are the major reservoirs of this diversity, and many important environments around us, as the Brazilian biomes Caatinga and Atlantic Forest, are poorly studied. Thus, the genetic material from environmental soil samples of Caatinga and Atlantic Forest biomes were extracted by direct techniques, pyrosequenced, and the sequences generated were analyzed by bioinformatics programs (MEGAN MG-RAST and WEBCarma). Taxonomic comparative profiles of the samples showed that the phyla Proteobacteria, Actinobacteria, Acidobacteria and Planctomycetes were the most representative. In addition, fungi of the phylum Ascomycota were identified predominantly in the soil sample from the Atlantic Forest. Metabolic profiles showed that despite the existence of environmental differences, sequences from both samples were similarly placed in the various functional subsystems, indicating no specific habitat functions. This work, a pioneer in taxonomic and metabolic comparative analysis of soil samples from Brazilian biomes, contributes to the knowledge of these complex environmental systems, so far little exploredOs microorganismos desempenham importantes funções na manutenção dos ecossistemas, o que explica o enorme interesse em compreender as relações existentes entre estes organismos, bem como entre eles e o meio. Estima-se que o número total de células procarióticas na Terra seja entre 4 e 6 x 1030, constituindo um enorme pool biológico e genético a ser explorado. Apesar de atualmente apenas 1% de toda essa riqueza poder ser cultivada por técnicas laboratoriais padrão, ferramentas metagenômicas permitem o acesso ao potencial genômico de amostras ambientais de forma independente de cultivo, e em associação com tecnologias de sequenciamento da terceira geração, a cobertura amostral se torna ainda maior. Solos, em particular, são os maiores reservatórios dessa diversidade, e muitos ambientes importantes ao nosso redor, como os biomas brasileiros Caatinga e Mata Atlântica, são pouco estudados. Sendo assim, o material genético ambiental de amostras de solo dos biomas Caatinga e Mata Atlântica foi extraído através de técnicas diretas, pirosequenciado, e as seqüências geradas foram analisadas através de programas de bioinformática (MEGAN, MG-RAST e WEBCarma). Perfis taxonômicos comparativos das amostras mostraram que os filos Proteobacteria, Actinobacteria, Acidobacteria e Planctomycetes foram os mais representativos. Em adição, fungos do filo Ascomycota foram identificados predominantemente na amostra de solo de Mata Atlântica. Perfis metabólicos mostraram que, apesar da existência de diferenças ambientais, sequências de ambas as amostras foram inseridas similarmente nos diversos subsistemas funcionais, não indicando funções habitat específicas. Este trabalho, pioneiro em análises taxonômicas e metabólicas comparativas de amostras de solo de biomas brasileiros, contribui para o conhecimento destes sistemas ambientais complexos, até então pouco exploradosConselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal do Rio Grande do NortePrograma de Pós-Graduação em BioquímicaUFRNBRBioquímica; Biologia MolecularMetagenomaSoloPirosequenciamentoMEGANCARMAMG-RASTCaatingaMata atlânticaMetagenomeSoilPyrosequencingMEGANCARMAMG-RASTCaatingaAtlantic ForestCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAMetagenômica comparativa de solo de regiões de mata atlântica e caatinga do estado do Rio Grande do Norte - Brasilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALMetagenômicaComparativaSolo_Pacchioni_2010.pdfMetagenômicaComparativaSolo_Pacchioni_2010.pdfapplication/pdf2829208https://repositorio.ufrn.br/bitstream/123456789/12572/1/Metagen%c3%b4micaComparativaSolo_Pacchioni_2010.pdfdeba80981c6a4c4836e1fd9c4c7b3f46MD51TEXTMetagenômicaComparativaSolo_Pacchioni_2010.pdf.txtMetagenômicaComparativaSolo_Pacchioni_2010.pdf.txtExtracted texttext/plain210535https://repositorio.ufrn.br/bitstream/123456789/12572/6/Metagen%c3%b4micaComparativaSolo_Pacchioni_2010.pdf.txt4cad23ceb735b3405a0761aa0762ac4eMD56THUMBNAILMetagenômicaComparativaSolo_Pacchioni_2010.pdf.jpgMetagenômicaComparativaSolo_Pacchioni_2010.pdf.jpgIM Thumbnailimage/jpeg4385https://repositorio.ufrn.br/bitstream/123456789/12572/7/Metagen%c3%b4micaComparativaSolo_Pacchioni_2010.pdf.jpgc2ef153098935b0d2959ca21c4e889f7MD57123456789/125722018-12-07 08:47:27.478oai:https://repositorio.ufrn.br:123456789/12572Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2018-12-07T11:47:27Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.por.fl_str_mv |
Metagenômica comparativa de solo de regiões de mata atlântica e caatinga do estado do Rio Grande do Norte - Brasil |
title |
Metagenômica comparativa de solo de regiões de mata atlântica e caatinga do estado do Rio Grande do Norte - Brasil |
spellingShingle |
Metagenômica comparativa de solo de regiões de mata atlântica e caatinga do estado do Rio Grande do Norte - Brasil Pacchioni, Ralfo Goes Metagenoma Solo Pirosequenciamento MEGAN CARMA MG-RAST Caatinga Mata atlântica Metagenome Soil Pyrosequencing MEGAN CARMA MG-RAST Caatinga Atlantic Forest CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Metagenômica comparativa de solo de regiões de mata atlântica e caatinga do estado do Rio Grande do Norte - Brasil |
title_full |
Metagenômica comparativa de solo de regiões de mata atlântica e caatinga do estado do Rio Grande do Norte - Brasil |
title_fullStr |
Metagenômica comparativa de solo de regiões de mata atlântica e caatinga do estado do Rio Grande do Norte - Brasil |
title_full_unstemmed |
Metagenômica comparativa de solo de regiões de mata atlântica e caatinga do estado do Rio Grande do Norte - Brasil |
title_sort |
Metagenômica comparativa de solo de regiões de mata atlântica e caatinga do estado do Rio Grande do Norte - Brasil |
author |
Pacchioni, Ralfo Goes |
author_facet |
Pacchioni, Ralfo Goes |
author_role |
author |
dc.contributor.authorID.por.fl_str_mv |
|
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/3944746730129166 |
dc.contributor.advisorID.por.fl_str_mv |
|
dc.contributor.advisorLattes.por.fl_str_mv |
http://lattes.cnpq.br/1083882171718362 |
dc.contributor.referees1.pt_BR.fl_str_mv |
Araújo, Magnólia Fernandes Florêncio de |
dc.contributor.referees1ID.por.fl_str_mv |
|
dc.contributor.referees1Lattes.por.fl_str_mv |
http://lattes.cnpq.br/4511989641459455 |
dc.contributor.referees2.pt_BR.fl_str_mv |
Melo, Vânia Maria Maciel |
dc.contributor.referees2ID.por.fl_str_mv |
|
dc.contributor.referees2Lattes.por.fl_str_mv |
http://lattes.cnpq.br/1572504650930605 |
dc.contributor.author.fl_str_mv |
Pacchioni, Ralfo Goes |
dc.contributor.advisor1.fl_str_mv |
Lima, Lucymara Fassarela Agnez |
contributor_str_mv |
Lima, Lucymara Fassarela Agnez |
dc.subject.por.fl_str_mv |
Metagenoma Solo Pirosequenciamento MEGAN CARMA MG-RAST Caatinga Mata atlântica |
topic |
Metagenoma Solo Pirosequenciamento MEGAN CARMA MG-RAST Caatinga Mata atlântica Metagenome Soil Pyrosequencing MEGAN CARMA MG-RAST Caatinga Atlantic Forest CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
dc.subject.eng.fl_str_mv |
Metagenome Soil Pyrosequencing MEGAN CARMA MG-RAST Caatinga Atlantic Forest |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
The microorganisms play very important roles in maintaining ecosystems, which explains the enormous interest in understanding the relationship between these organisms as well as between them and the environment. It is estimated that the total number of prokaryotic cells on Earth is between 4 and 6 x 1030, constituting an enormous biological and genetic pool to be explored. Although currently only 1% of all this wealth can be cultivated by standard laboratory techniques, metagenomic tools allow access to the genomic potential of environmental samples in a independent culture manner, and in combination with third generation sequencing technologies, the samples coverage become even greater. Soils, in particular, are the major reservoirs of this diversity, and many important environments around us, as the Brazilian biomes Caatinga and Atlantic Forest, are poorly studied. Thus, the genetic material from environmental soil samples of Caatinga and Atlantic Forest biomes were extracted by direct techniques, pyrosequenced, and the sequences generated were analyzed by bioinformatics programs (MEGAN MG-RAST and WEBCarma). Taxonomic comparative profiles of the samples showed that the phyla Proteobacteria, Actinobacteria, Acidobacteria and Planctomycetes were the most representative. In addition, fungi of the phylum Ascomycota were identified predominantly in the soil sample from the Atlantic Forest. Metabolic profiles showed that despite the existence of environmental differences, sequences from both samples were similarly placed in the various functional subsystems, indicating no specific habitat functions. This work, a pioneer in taxonomic and metabolic comparative analysis of soil samples from Brazilian biomes, contributes to the knowledge of these complex environmental systems, so far little explored |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010-12-02 |
dc.date.available.fl_str_mv |
2011-05-12 2014-12-17T14:03:36Z |
dc.date.accessioned.fl_str_mv |
2014-12-17T14:03:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PACCHIONI, Ralfo Goes. Metagenômica comparativa de solo de regiões de mata atlântica e caatinga do estado do Rio Grande do Norte - Brasil. 2010. 156 f. Dissertação (Mestrado em Bioquímica; Biologia Molecular) - Universidade Federal do Rio Grande do Norte, Natal, 2010. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/jspui/handle/123456789/12572 |
identifier_str_mv |
PACCHIONI, Ralfo Goes. Metagenômica comparativa de solo de regiões de mata atlântica e caatinga do estado do Rio Grande do Norte - Brasil. 2010. 156 f. Dissertação (Mestrado em Bioquímica; Biologia Molecular) - Universidade Federal do Rio Grande do Norte, Natal, 2010. |
url |
https://repositorio.ufrn.br/jspui/handle/123456789/12572 |
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por |
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por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Universidade Federal do Rio Grande do Norte |
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Programa de Pós-Graduação em Bioquímica |
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UFRN |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Bioquímica; Biologia Molecular |
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Universidade Federal do Rio Grande do Norte |
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