Variação no conteúdo protéico e pigmentar em variantes cromáticas de Gracilaria domingensis nas populações naturais de Rio do Fogo-RN, BRASIL
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/jspui/handle/123456789/12482 |
Resumo: | The seaweed Gracilaria domingensis is a common species in the coast of Rio Grande do Norte. This species lives in the intertidal zone, where colour strains (red, green and brown) co-occur during the whole year. Seaweeds that live in this region are exposed to daily changes and to the rhythm of the tide. During the low tide they are exposed to dissection, hiper-or hipo-osmotic shock, high temperatures and high irradiance. The aim of this study was to analyze whether the pigment and protein content of the colour strains of G. domingensis is affected by some environmental parameters in a temporal scale. The seaweeds were collected during 10 months in the seashore of Rio do Fogo (RN). The total soluble proteins and the phycobiliprotein were extracted in phosphate buffer and the carotenoids were analyzed by a standardized method through HPLC-UV. The pigments analysis showed that phycoerithrin is the most abundant pigment in the three strains. This pigment was strongly correlated with nitrogen and the photosynthetically active radiation. Chlorophyll presented higher concentrations than carotenoids during the whole, but the ratio carotenoid/chlorophyll-a was modified by incident radiation. The most abundant carotenoid was ß-carotene and zeaxanthin, which had higher concentrations in the higher radiation months. The concentration increase of zeaxanthin in this period indicated a photoprotective response of the seaweed. The three strains presented a pigment profile that indicates different radiation tolerance profile. Our results pointed that the green strain is better adapted to high irradiance levels than the red and brown strains |
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Pereira, Dinaelza Castelohttp://lattes.cnpq.br/3285816862767001http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786301Y8&dataRevisao=nullColepicolo Neto, Piohttp://lattes.cnpq.br/9346462712672265Amado, André Megalihttp://lattes.cnpq.br/4312158184208542Câmara, Marcos Rogériohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783008H5Soriano, Eliane Marinho2014-12-17T14:01:58Z2009-05-192014-12-17T14:01:58Z2009-03-23PEREIRA, Dinaelza Castelo. Variation in protein and pigment content in colour strains of Gracilaria domingensis in the natural populations of Rio do Fogo (RN). 2009. 91 f. Dissertação (Mestrado em Bioecologia Aquática) - Universidade Federal do Rio Grande do Norte, Natal, 2009.https://repositorio.ufrn.br/jspui/handle/123456789/12482The seaweed Gracilaria domingensis is a common species in the coast of Rio Grande do Norte. This species lives in the intertidal zone, where colour strains (red, green and brown) co-occur during the whole year. Seaweeds that live in this region are exposed to daily changes and to the rhythm of the tide. During the low tide they are exposed to dissection, hiper-or hipo-osmotic shock, high temperatures and high irradiance. The aim of this study was to analyze whether the pigment and protein content of the colour strains of G. domingensis is affected by some environmental parameters in a temporal scale. The seaweeds were collected during 10 months in the seashore of Rio do Fogo (RN). The total soluble proteins and the phycobiliprotein were extracted in phosphate buffer and the carotenoids were analyzed by a standardized method through HPLC-UV. The pigments analysis showed that phycoerithrin is the most abundant pigment in the three strains. This pigment was strongly correlated with nitrogen and the photosynthetically active radiation. Chlorophyll presented higher concentrations than carotenoids during the whole, but the ratio carotenoid/chlorophyll-a was modified by incident radiation. The most abundant carotenoid was ß-carotene and zeaxanthin, which had higher concentrations in the higher radiation months. The concentration increase of zeaxanthin in this period indicated a photoprotective response of the seaweed. The three strains presented a pigment profile that indicates different radiation tolerance profile. Our results pointed that the green strain is better adapted to high irradiance levels than the red and brown strainsA macroalga Gracilaria domingensis é comumente encontrada no litoral do Rio Grande do Norte. Esta espécie habita a zona intertidal, onde linhagens cromáticas (vermelha, verde e marrom) co-ocorrem durante todo o ano. Algas que vivem nesta região estão submetidas a mudanças diurnas e ao ritmo da maré. Durante a maré baixa elas ficam expostas a dissecação, choque hiper ou hipo-osmótico, altas temperaturas e elevada irradiância. Este trabalho teve como objetivo geral o estudo do perfil protéico e pigmentar das variantes cromáticas de G. domingensis e a alteração deste perfil em função dos parâmetros ambientais em uma escala temporal. As algas foram coletadas mensalmente, durante 10 meses, nas parias de Rio do Fogo-RN. As proteínas totais solúveis e as ficobiliproteínas foram extraídas em tampão fosfato e os carotenóides foram analisados em um método padronizado por HPLC-UV. A análise dos pigmentos mostrou que a ficoeritrina é o pigmento mais abundante nas três linhagens. Este pigmento esteve fortemente correlacionado com o nitrogênio e a PAR. Os pigmentos carotenóides apresentaram concentrações inferiores as da clorofila-a durante todos os meses, mas a razão carotenóides/clorofila-a foi modificada com o aumento da radiação. O carotenóide mais abundante foi o ß-caroteno, seguido da zeaxantina, que esteve em maiores concentrações nos meses de maior radiação. O aumento na concentração da zeaxantina nesse período indicou uma resposta fotoprotetora da alga. As três linhagens apresentaram um perfil pigmentar que remete a diferentes padrões de tolerância a radiação. A linhagem verde mostrou ser melhor adaptada a elevados níveis de irradiâncias do que a vermelha e a marromCoordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal do Rio Grande do NortePrograma de Pós-Graduação em Bioecologia AquáticaUFRNBRBioecologia AquáticaGracilaria domingensisLinhagensEstresse ambientalProteínasFicobiliproteínasCarotenóidesGracilaria domingensisStrainsEnvironmental stressProteinPhycobiliproteinCarotenoidsCNPQ::CIENCIAS BIOLOGICAS::ECOLOGIAVariação no conteúdo protéico e pigmentar em variantes cromáticas de Gracilaria domingensis nas populações naturais de Rio do Fogo-RN, BRASILVariation in protein and pigment content in colour strains of Gracilaria domingensis in the natural populations of Rio do Fogo (RN)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNTEXTDinaelzaCP.pdf.txtDinaelzaCP.pdf.txtExtracted texttext/plain132891https://repositorio.ufrn.br/bitstream/123456789/12482/6/DinaelzaCP.pdf.txt7110fd8dd1d8eecb051c692d54a8a803MD56VariacaoConteúdoProtéico_Pereira_2009.pdf.txtVariacaoConteúdoProtéico_Pereira_2009.pdf.txtExtracted texttext/plain132891https://repositorio.ufrn.br/bitstream/123456789/12482/8/VariacaoConte%c3%badoProt%c3%a9ico_Pereira_2009.pdf.txt7110fd8dd1d8eecb051c692d54a8a803MD58THUMBNAILDinaelzaCP.pdf.jpgDinaelzaCP.pdf.jpgIM Thumbnailimage/jpeg3304https://repositorio.ufrn.br/bitstream/123456789/12482/7/DinaelzaCP.pdf.jpg1bc4f66ad4d7f628b80419e4635ef561MD57VariacaoConteúdoProtéico_Pereira_2009.pdf.jpgVariacaoConteúdoProtéico_Pereira_2009.pdf.jpgIM Thumbnailimage/jpeg3304https://repositorio.ufrn.br/bitstream/123456789/12482/9/VariacaoConte%c3%badoProt%c3%a9ico_Pereira_2009.pdf.jpg1bc4f66ad4d7f628b80419e4635ef561MD59ORIGINALVariacaoConteúdoProtéico_Pereira_2009.pdfapplication/pdf982597https://repositorio.ufrn.br/bitstream/123456789/12482/1/VariacaoConte%c3%badoProt%c3%a9ico_Pereira_2009.pdf8505a456042640660a4eeec39570774fMD51123456789/124822019-01-30 06:22:36.137oai:https://repositorio.ufrn.br:123456789/12482Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2019-01-30T09:22:36Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.por.fl_str_mv |
Variação no conteúdo protéico e pigmentar em variantes cromáticas de Gracilaria domingensis nas populações naturais de Rio do Fogo-RN, BRASIL |
dc.title.alternative.eng.fl_str_mv |
Variation in protein and pigment content in colour strains of Gracilaria domingensis in the natural populations of Rio do Fogo (RN) |
title |
Variação no conteúdo protéico e pigmentar em variantes cromáticas de Gracilaria domingensis nas populações naturais de Rio do Fogo-RN, BRASIL |
spellingShingle |
Variação no conteúdo protéico e pigmentar em variantes cromáticas de Gracilaria domingensis nas populações naturais de Rio do Fogo-RN, BRASIL Pereira, Dinaelza Castelo Gracilaria domingensis Linhagens Estresse ambiental Proteínas Ficobiliproteínas Carotenóides Gracilaria domingensis Strains Environmental stress Protein Phycobiliprotein Carotenoids CNPQ::CIENCIAS BIOLOGICAS::ECOLOGIA |
title_short |
Variação no conteúdo protéico e pigmentar em variantes cromáticas de Gracilaria domingensis nas populações naturais de Rio do Fogo-RN, BRASIL |
title_full |
Variação no conteúdo protéico e pigmentar em variantes cromáticas de Gracilaria domingensis nas populações naturais de Rio do Fogo-RN, BRASIL |
title_fullStr |
Variação no conteúdo protéico e pigmentar em variantes cromáticas de Gracilaria domingensis nas populações naturais de Rio do Fogo-RN, BRASIL |
title_full_unstemmed |
Variação no conteúdo protéico e pigmentar em variantes cromáticas de Gracilaria domingensis nas populações naturais de Rio do Fogo-RN, BRASIL |
title_sort |
Variação no conteúdo protéico e pigmentar em variantes cromáticas de Gracilaria domingensis nas populações naturais de Rio do Fogo-RN, BRASIL |
author |
Pereira, Dinaelza Castelo |
author_facet |
Pereira, Dinaelza Castelo |
author_role |
author |
dc.contributor.authorID.por.fl_str_mv |
|
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/3285816862767001 |
dc.contributor.advisorID.por.fl_str_mv |
|
dc.contributor.advisorLattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786301Y8&dataRevisao=null |
dc.contributor.referees1.pt_BR.fl_str_mv |
Colepicolo Neto, Pio |
dc.contributor.referees1ID.por.fl_str_mv |
|
dc.contributor.referees1Lattes.por.fl_str_mv |
http://lattes.cnpq.br/9346462712672265 |
dc.contributor.referees2.pt_BR.fl_str_mv |
Amado, André Megali |
dc.contributor.referees2ID.por.fl_str_mv |
|
dc.contributor.referees2Lattes.por.fl_str_mv |
http://lattes.cnpq.br/4312158184208542 |
dc.contributor.referees3.pt_BR.fl_str_mv |
Câmara, Marcos Rogério |
dc.contributor.referees3ID.por.fl_str_mv |
|
dc.contributor.referees3Lattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783008H5 |
dc.contributor.author.fl_str_mv |
Pereira, Dinaelza Castelo |
dc.contributor.advisor1.fl_str_mv |
Soriano, Eliane Marinho |
contributor_str_mv |
Soriano, Eliane Marinho |
dc.subject.por.fl_str_mv |
Gracilaria domingensis Linhagens Estresse ambiental Proteínas Ficobiliproteínas Carotenóides |
topic |
Gracilaria domingensis Linhagens Estresse ambiental Proteínas Ficobiliproteínas Carotenóides Gracilaria domingensis Strains Environmental stress Protein Phycobiliprotein Carotenoids CNPQ::CIENCIAS BIOLOGICAS::ECOLOGIA |
dc.subject.eng.fl_str_mv |
Gracilaria domingensis Strains Environmental stress Protein Phycobiliprotein Carotenoids |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::ECOLOGIA |
description |
The seaweed Gracilaria domingensis is a common species in the coast of Rio Grande do Norte. This species lives in the intertidal zone, where colour strains (red, green and brown) co-occur during the whole year. Seaweeds that live in this region are exposed to daily changes and to the rhythm of the tide. During the low tide they are exposed to dissection, hiper-or hipo-osmotic shock, high temperatures and high irradiance. The aim of this study was to analyze whether the pigment and protein content of the colour strains of G. domingensis is affected by some environmental parameters in a temporal scale. The seaweeds were collected during 10 months in the seashore of Rio do Fogo (RN). The total soluble proteins and the phycobiliprotein were extracted in phosphate buffer and the carotenoids were analyzed by a standardized method through HPLC-UV. The pigments analysis showed that phycoerithrin is the most abundant pigment in the three strains. This pigment was strongly correlated with nitrogen and the photosynthetically active radiation. Chlorophyll presented higher concentrations than carotenoids during the whole, but the ratio carotenoid/chlorophyll-a was modified by incident radiation. The most abundant carotenoid was ß-carotene and zeaxanthin, which had higher concentrations in the higher radiation months. The concentration increase of zeaxanthin in this period indicated a photoprotective response of the seaweed. The three strains presented a pigment profile that indicates different radiation tolerance profile. Our results pointed that the green strain is better adapted to high irradiance levels than the red and brown strains |
publishDate |
2009 |
dc.date.available.fl_str_mv |
2009-05-19 2014-12-17T14:01:58Z |
dc.date.issued.fl_str_mv |
2009-03-23 |
dc.date.accessioned.fl_str_mv |
2014-12-17T14:01:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PEREIRA, Dinaelza Castelo. Variation in protein and pigment content in colour strains of Gracilaria domingensis in the natural populations of Rio do Fogo (RN). 2009. 91 f. Dissertação (Mestrado em Bioecologia Aquática) - Universidade Federal do Rio Grande do Norte, Natal, 2009. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/jspui/handle/123456789/12482 |
identifier_str_mv |
PEREIRA, Dinaelza Castelo. Variation in protein and pigment content in colour strains of Gracilaria domingensis in the natural populations of Rio do Fogo (RN). 2009. 91 f. Dissertação (Mestrado em Bioecologia Aquática) - Universidade Federal do Rio Grande do Norte, Natal, 2009. |
url |
https://repositorio.ufrn.br/jspui/handle/123456789/12482 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal do Rio Grande do Norte |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Bioecologia Aquática |
dc.publisher.initials.fl_str_mv |
UFRN |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Bioecologia Aquática |
publisher.none.fl_str_mv |
Universidade Federal do Rio Grande do Norte |
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reponame:Repositório Institucional da UFRN instname:Universidade Federal do Rio Grande do Norte (UFRN) instacron:UFRN |
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UFRN |
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UFRN |
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Repositório Institucional da UFRN |
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