Simvastatin improves the healing of infected skin wounds of rats

Detalhes bibliográficos
Autor(a) principal: Rego, Amália Cínthia Meneses do
Data de Publicação: 2007
Outros Autores: Araújo Filho, Irami, Damasceno, Bolívar P G L, Egito, Eryvaldo Sócrates Tabosa, Silveira, Ivanaldo Amâncio da, Brandão Neto, José, Medeiros, Aldo Cunha
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/jspui/handle/1/6290
Resumo: This study explores the potential of the simvastatin to ameliorate inflammation and infection in open infected skin wounds of rats. Methods: Fourteen Wistar rats weighing 285±12g were used. The study was done in a group whose open infected skin wounds were treated with topical application of sinvastatina microemulsion (SIM, n=7) and a second group with wounds treated with saline 0.9 % (SAL, n=7). A bacteriological exam of the wounds fluid for gram positive and gram negative bacteria, the tecidual expression of TNFá and IL-1â by imunohistochemical technique, and histological analysis by HE stain were performed. Results: The expression of TNFa could be clearly demonstrated in lower degree in skin wounds treated with simvastatin (668.6 ± 74.7 ìm2) than in saline (2120.0 ± 327.1 ìm2). In comparison, wound tissue from SIM group displayed leukocyte infiltration significantly lower than that observed in SAL group (p<0.05). Culture results of the samples taken from wound fluid on fourth post treatment day revealed wound infection in only one rat of group simvastatin (SIM), where Proteus mirabilis, Escherchia coli and Enterobacter sp were isolated. In the rats whose wounds were treated with saline (SAL), polymicrobial infection with more than 100,000 CFU/g was detected in all the wounds. Conclusion: In addition to its antiinflammatory properties, the protective effects of simvastatin in infected open skin wounds is able to reduce infection and probably has antibacterial action. The potential to treat these wounds with statins to ameliorate inflammation and infection is promising
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spelling Rego, Amália Cínthia Meneses doAraújo Filho, IramiDamasceno, Bolívar P G LEgito, Eryvaldo Sócrates TabosaSilveira, Ivanaldo Amâncio daBrandão Neto, JoséMedeiros, Aldo Cunha2013-09-30T15:23:29Z2013-09-30T15:23:29Z2007REGO, Amália Cínthia Meneses do ; ARAÚJO FILHO, Irami ; DAMASCENO, Bolívar P G L ; EGITO, Eryvaldo Sócrates Tabosa ; SILVEIRA, Ivanaldo Amâncio da ; BRANDÃO NETO, José ; MEDEIROS, Aldo Cunha. Simvastatin improves the healing of infected skin wounds of rats. Acta Cirúrgica Brasileira , v. 22, p. 57-63, 2007. Disponível em: <http://www.scielo.br/scielo.php?pid=s0102-86502007000700012&script=sci_arttext> Acesso em: 19 set. 2013.1678-2674https://repositorio.ufrn.br/jspui/handle/1/6290This study explores the potential of the simvastatin to ameliorate inflammation and infection in open infected skin wounds of rats. Methods: Fourteen Wistar rats weighing 285±12g were used. The study was done in a group whose open infected skin wounds were treated with topical application of sinvastatina microemulsion (SIM, n=7) and a second group with wounds treated with saline 0.9 % (SAL, n=7). A bacteriological exam of the wounds fluid for gram positive and gram negative bacteria, the tecidual expression of TNFá and IL-1â by imunohistochemical technique, and histological analysis by HE stain were performed. Results: The expression of TNFa could be clearly demonstrated in lower degree in skin wounds treated with simvastatin (668.6 ± 74.7 ìm2) than in saline (2120.0 ± 327.1 ìm2). In comparison, wound tissue from SIM group displayed leukocyte infiltration significantly lower than that observed in SAL group (p<0.05). Culture results of the samples taken from wound fluid on fourth post treatment day revealed wound infection in only one rat of group simvastatin (SIM), where Proteus mirabilis, Escherchia coli and Enterobacter sp were isolated. In the rats whose wounds were treated with saline (SAL), polymicrobial infection with more than 100,000 CFU/g was detected in all the wounds. Conclusion: In addition to its antiinflammatory properties, the protective effects of simvastatin in infected open skin wounds is able to reduce infection and probably has antibacterial action. The potential to treat these wounds with statins to ameliorate inflammation and infection is promisingengStatinInflammationWound HealingWistar ratSkinCytokineSimvastatin improves the healing of infected skin wounds of ratsA sinvastatina melhora a cicatrização de feridas infectadas da pele de ratosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALSimvastatinImprovesHealing_Rego_2007.pdfSimvastatinImprovesHealing_Rego_2007.pdfapplication/pdf146250https://repositorio.ufrn.br/bitstream/1/6290/1/SimvastatinImprovesHealing_Rego_2007.pdf027cb4735d15fee0d71c715306193f2fMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-823898https://repositorio.ufrn.br/bitstream/1/6290/2/license_rdfe363e809996cf46ada20da1accfcd9c7MD52license_textlicense_texttext/html; charset=utf-80https://repositorio.ufrn.br/bitstream/1/6290/3/license_textd41d8cd98f00b204e9800998ecf8427eMD53license_urllicense_urltext/plain; charset=utf-852https://repositorio.ufrn.br/bitstream/1/6290/4/license_url3d480ae6c91e310daba2020f8787d6f9MD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.ufrn.br/bitstream/1/6290/5/license.txt8a4605be74aa9ea9d79846c1fba20a33MD55TEXTAldoCM_Simvastatin_12.pdf.txtAldoCM_Simvastatin_12.pdf.txtExtracted texttext/plain26441https://repositorio.ufrn.br/bitstream/1/6290/10/AldoCM_Simvastatin_12.pdf.txtd9893dc373ae2a45bea90aa107355343MD510THUMBNAILAldoCM_Simvastatin_12.pdf.jpgAldoCM_Simvastatin_12.pdf.jpgIM Thumbnailimage/jpeg8167https://repositorio.ufrn.br/bitstream/1/6290/11/AldoCM_Simvastatin_12.pdf.jpgdf9bc16bb4ae12205424b5c86726ab08MD5111/62902021-11-30 17:28:31.357oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2021-11-30T20:28:31Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.pt_BR.fl_str_mv Simvastatin improves the healing of infected skin wounds of rats
dc.title.alternative.pt_BR.fl_str_mv A sinvastatina melhora a cicatrização de feridas infectadas da pele de ratos
title Simvastatin improves the healing of infected skin wounds of rats
spellingShingle Simvastatin improves the healing of infected skin wounds of rats
Rego, Amália Cínthia Meneses do
Statin
Inflammation
Wound Healing
Wistar rat
Skin
Cytokine
title_short Simvastatin improves the healing of infected skin wounds of rats
title_full Simvastatin improves the healing of infected skin wounds of rats
title_fullStr Simvastatin improves the healing of infected skin wounds of rats
title_full_unstemmed Simvastatin improves the healing of infected skin wounds of rats
title_sort Simvastatin improves the healing of infected skin wounds of rats
author Rego, Amália Cínthia Meneses do
author_facet Rego, Amália Cínthia Meneses do
Araújo Filho, Irami
Damasceno, Bolívar P G L
Egito, Eryvaldo Sócrates Tabosa
Silveira, Ivanaldo Amâncio da
Brandão Neto, José
Medeiros, Aldo Cunha
author_role author
author2 Araújo Filho, Irami
Damasceno, Bolívar P G L
Egito, Eryvaldo Sócrates Tabosa
Silveira, Ivanaldo Amâncio da
Brandão Neto, José
Medeiros, Aldo Cunha
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rego, Amália Cínthia Meneses do
Araújo Filho, Irami
Damasceno, Bolívar P G L
Egito, Eryvaldo Sócrates Tabosa
Silveira, Ivanaldo Amâncio da
Brandão Neto, José
Medeiros, Aldo Cunha
dc.subject.por.fl_str_mv Statin
Inflammation
Wound Healing
Wistar rat
Skin
Cytokine
topic Statin
Inflammation
Wound Healing
Wistar rat
Skin
Cytokine
description This study explores the potential of the simvastatin to ameliorate inflammation and infection in open infected skin wounds of rats. Methods: Fourteen Wistar rats weighing 285±12g were used. The study was done in a group whose open infected skin wounds were treated with topical application of sinvastatina microemulsion (SIM, n=7) and a second group with wounds treated with saline 0.9 % (SAL, n=7). A bacteriological exam of the wounds fluid for gram positive and gram negative bacteria, the tecidual expression of TNFá and IL-1â by imunohistochemical technique, and histological analysis by HE stain were performed. Results: The expression of TNFa could be clearly demonstrated in lower degree in skin wounds treated with simvastatin (668.6 ± 74.7 ìm2) than in saline (2120.0 ± 327.1 ìm2). In comparison, wound tissue from SIM group displayed leukocyte infiltration significantly lower than that observed in SAL group (p<0.05). Culture results of the samples taken from wound fluid on fourth post treatment day revealed wound infection in only one rat of group simvastatin (SIM), where Proteus mirabilis, Escherchia coli and Enterobacter sp were isolated. In the rats whose wounds were treated with saline (SAL), polymicrobial infection with more than 100,000 CFU/g was detected in all the wounds. Conclusion: In addition to its antiinflammatory properties, the protective effects of simvastatin in infected open skin wounds is able to reduce infection and probably has antibacterial action. The potential to treat these wounds with statins to ameliorate inflammation and infection is promising
publishDate 2007
dc.date.issued.fl_str_mv 2007
dc.date.accessioned.fl_str_mv 2013-09-30T15:23:29Z
dc.date.available.fl_str_mv 2013-09-30T15:23:29Z
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dc.identifier.citation.fl_str_mv REGO, Amália Cínthia Meneses do ; ARAÚJO FILHO, Irami ; DAMASCENO, Bolívar P G L ; EGITO, Eryvaldo Sócrates Tabosa ; SILVEIRA, Ivanaldo Amâncio da ; BRANDÃO NETO, José ; MEDEIROS, Aldo Cunha. Simvastatin improves the healing of infected skin wounds of rats. Acta Cirúrgica Brasileira , v. 22, p. 57-63, 2007. Disponível em: <http://www.scielo.br/scielo.php?pid=s0102-86502007000700012&script=sci_arttext> Acesso em: 19 set. 2013.
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/jspui/handle/1/6290
dc.identifier.issn.none.fl_str_mv 1678-2674
identifier_str_mv REGO, Amália Cínthia Meneses do ; ARAÚJO FILHO, Irami ; DAMASCENO, Bolívar P G L ; EGITO, Eryvaldo Sócrates Tabosa ; SILVEIRA, Ivanaldo Amâncio da ; BRANDÃO NETO, José ; MEDEIROS, Aldo Cunha. Simvastatin improves the healing of infected skin wounds of rats. Acta Cirúrgica Brasileira , v. 22, p. 57-63, 2007. Disponível em: <http://www.scielo.br/scielo.php?pid=s0102-86502007000700012&script=sci_arttext> Acesso em: 19 set. 2013.
1678-2674
url https://repositorio.ufrn.br/jspui/handle/1/6290
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