Screening of L-Asparaginase the salt-tolerant and thermostable marine Bacillus Subtilis Strain SR61

Detalhes bibliográficos
Autor(a) principal: Veras, Bruno Oliveira de
Data de Publicação: 2019
Outros Autores: Santos, Yago Queiroz dos, França, Anderson Felipe Jácome de, Ribeiro, Penha Patricia Cabral, Barbosa, Elaine Costa Almeida, Gorlach-Lira, Krystyna
Tipo de documento: Capítulo de livro
Idioma: eng
Título da fonte: Repositório Institucional da UFRN
DOI: 10.22533/at.ed.732191303
Texto Completo: https://repositorio.ufrn.br/handle/123456789/32804
Resumo: The marine environment harbors different microorganisms that inhabit niches with adverse conditions, such as variation of temperature, pressure and salinity. To survive these particular conditions, marine bacteria use unique metabolic and biochemical characteristics, producing enzymes that have high industrial value. The objective of this study was to observe the production of thermostable and halotolerant L-asparaginase by marine bacteria isolated from the coral reefs of Cabo Branco, State of Paraíba, Brazil. An L-asparaginase-producing bacterial isolate was identified and identified by phylogenetic analysis as Bacillus subtilis SR61, via a 16S ribosomal RNA assay. For screening of L-asparaginase by SR61, it was inoculated in a differential medium to induce the production of salt tolerant extracellular thermostable enzyme with the addition of increasing salt concentrations (0, 0.5, 1.0, 1.5 e 2.0 M NaCl) by 55 0C for 24 hours. The screening showed a capacity of halotolerant and thermostable L-asparaginase production by the isolate identified as Bacillus subtilis, the production being limited to 1.0 M salt, having a total activity (IU / mL) 231.4 ± 3.57 and specifying IU / μg 8.39. Bacillus subtilis SR61 was able to produce L-asparaginase when submitted to a high salinity environment, demonstrating the halophytic nature of the isolate, having several applications in several industrial branches
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spelling Veras, Bruno Oliveira deSantos, Yago Queiroz dosFrança, Anderson Felipe Jácome deRibeiro, Penha Patricia CabralBarbosa, Elaine Costa AlmeidaGorlach-Lira, Krystyna2021-07-05T13:09:31Z2021-07-05T13:09:31Z2019VERAS, Bruno Oliveira de et al. Screening of L-Asparaginase the salt-tolerant and thermostable marine Bacillus Subtilis Strain SR61. In: LUZ, Patrícia Michele da (Org.). Biological Sciences Foudantions. 1. ed. Ponta Grossa, PR: Atena Editora, 2019. p. 93-100978-85-7247-173-2https://repositorio.ufrn.br/handle/123456789/3280410.22533/at.ed.732191303The marine environment harbors different microorganisms that inhabit niches with adverse conditions, such as variation of temperature, pressure and salinity. To survive these particular conditions, marine bacteria use unique metabolic and biochemical characteristics, producing enzymes that have high industrial value. The objective of this study was to observe the production of thermostable and halotolerant L-asparaginase by marine bacteria isolated from the coral reefs of Cabo Branco, State of Paraíba, Brazil. An L-asparaginase-producing bacterial isolate was identified and identified by phylogenetic analysis as Bacillus subtilis SR61, via a 16S ribosomal RNA assay. For screening of L-asparaginase by SR61, it was inoculated in a differential medium to induce the production of salt tolerant extracellular thermostable enzyme with the addition of increasing salt concentrations (0, 0.5, 1.0, 1.5 e 2.0 M NaCl) by 55 0C for 24 hours. The screening showed a capacity of halotolerant and thermostable L-asparaginase production by the isolate identified as Bacillus subtilis, the production being limited to 1.0 M salt, having a total activity (IU / mL) 231.4 ± 3.57 and specifying IU / μg 8.39. Bacillus subtilis SR61 was able to produce L-asparaginase when submitted to a high salinity environment, demonstrating the halophytic nature of the isolate, having several applications in several industrial branchesO ambiente marinho abriga diferentes microorganismos que habitam nichos com condições adversas, como variação de temperatura, pressão e salinidade. Para sobreviver a essas condições particulares, as bactérias marinhas usam características metabólicas e bioquímicas únicas, produzindo enzimas que possuem elevado valor industrial. O objetivo deste estudo foi observar a produção de L-asparaginase termoestável e halotolerante por bacterias marinhas isolado dos recifes de coral do Cabo Branco, Estado da Paraíba, Brasil. Foi obtido um isolado bacteriano produtor de L-asparaginase, sendo identificado identificada por análise filogenética como sendo Bacillus subtilis SR61, através de um ensaio de RNA ribossómico 16S. Para a triagem de L-asparaginase por SR61, foi inoculado em meio diferencial para induzir a produção de enzima termostável extracelular tolerante ao sal com a adição de concentrações crescentes de sal (0, 0.5, 1.0, 1.5 e 2.0 M NaCl) em 55° C por 24 horas. A triagem mostrou capacidade de produção de L-asparaginase halotolerante e termoestável pelo isolado identificado como Bacillus subtilis, sendo a produção limitada a 1,0 M de sal, tendo como atividade total (UI / mL) 231,4 ± 3,57 e especifica IU/μg 8,39. Bacillus subtilis SR61 mostrou-se capaz de produzir L-asparaginase quando submetido a um ambiente de alta salinidade, demonstrando a natureza halofítica do isolado, tendo diversas aplicações em diversos ramos industriaisAtena EditoraL-AsparaginaseBactériaEnzyme activityBactériasAtividade enzimáticaScreening of L-Asparaginase the salt-tolerant and thermostable marine Bacillus Subtilis Strain SR61info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bookPartengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNinfo:eu-repo/semantics/openAccessORIGINALScreeningLAsparaginaseBacillus_Franca_2019.pdfScreeningLAsparaginaseBacillus_Franca_2019.pdfapplication/pdf346652https://repositorio.ufrn.br/bitstream/123456789/32804/1/ScreeningLAsparaginaseBacillus_Franca_2019.pdf7002dbbab9739679d39bc9405bc12814MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/32804/2/license.txte9597aa2854d128fd968be5edc8a28d9MD52123456789/328042022-04-12 14:30:20.806oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2022-04-12T17:30:20Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.pt_BR.fl_str_mv Screening of L-Asparaginase the salt-tolerant and thermostable marine Bacillus Subtilis Strain SR61
title Screening of L-Asparaginase the salt-tolerant and thermostable marine Bacillus Subtilis Strain SR61
spellingShingle Screening of L-Asparaginase the salt-tolerant and thermostable marine Bacillus Subtilis Strain SR61
Veras, Bruno Oliveira de
L-Asparaginase
Bactéria
Enzyme activity
Bactérias
Atividade enzimática
title_short Screening of L-Asparaginase the salt-tolerant and thermostable marine Bacillus Subtilis Strain SR61
title_full Screening of L-Asparaginase the salt-tolerant and thermostable marine Bacillus Subtilis Strain SR61
title_fullStr Screening of L-Asparaginase the salt-tolerant and thermostable marine Bacillus Subtilis Strain SR61
title_full_unstemmed Screening of L-Asparaginase the salt-tolerant and thermostable marine Bacillus Subtilis Strain SR61
title_sort Screening of L-Asparaginase the salt-tolerant and thermostable marine Bacillus Subtilis Strain SR61
author Veras, Bruno Oliveira de
author_facet Veras, Bruno Oliveira de
Santos, Yago Queiroz dos
França, Anderson Felipe Jácome de
Ribeiro, Penha Patricia Cabral
Barbosa, Elaine Costa Almeida
Gorlach-Lira, Krystyna
author_role author
author2 Santos, Yago Queiroz dos
França, Anderson Felipe Jácome de
Ribeiro, Penha Patricia Cabral
Barbosa, Elaine Costa Almeida
Gorlach-Lira, Krystyna
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Veras, Bruno Oliveira de
Santos, Yago Queiroz dos
França, Anderson Felipe Jácome de
Ribeiro, Penha Patricia Cabral
Barbosa, Elaine Costa Almeida
Gorlach-Lira, Krystyna
dc.subject.por.fl_str_mv L-Asparaginase
Bactéria
Enzyme activity
Bactérias
Atividade enzimática
topic L-Asparaginase
Bactéria
Enzyme activity
Bactérias
Atividade enzimática
description The marine environment harbors different microorganisms that inhabit niches with adverse conditions, such as variation of temperature, pressure and salinity. To survive these particular conditions, marine bacteria use unique metabolic and biochemical characteristics, producing enzymes that have high industrial value. The objective of this study was to observe the production of thermostable and halotolerant L-asparaginase by marine bacteria isolated from the coral reefs of Cabo Branco, State of Paraíba, Brazil. An L-asparaginase-producing bacterial isolate was identified and identified by phylogenetic analysis as Bacillus subtilis SR61, via a 16S ribosomal RNA assay. For screening of L-asparaginase by SR61, it was inoculated in a differential medium to induce the production of salt tolerant extracellular thermostable enzyme with the addition of increasing salt concentrations (0, 0.5, 1.0, 1.5 e 2.0 M NaCl) by 55 0C for 24 hours. The screening showed a capacity of halotolerant and thermostable L-asparaginase production by the isolate identified as Bacillus subtilis, the production being limited to 1.0 M salt, having a total activity (IU / mL) 231.4 ± 3.57 and specifying IU / μg 8.39. Bacillus subtilis SR61 was able to produce L-asparaginase when submitted to a high salinity environment, demonstrating the halophytic nature of the isolate, having several applications in several industrial branches
publishDate 2019
dc.date.issued.fl_str_mv 2019
dc.date.accessioned.fl_str_mv 2021-07-05T13:09:31Z
dc.date.available.fl_str_mv 2021-07-05T13:09:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/bookPart
format bookPart
status_str publishedVersion
dc.identifier.citation.fl_str_mv VERAS, Bruno Oliveira de et al. Screening of L-Asparaginase the salt-tolerant and thermostable marine Bacillus Subtilis Strain SR61. In: LUZ, Patrícia Michele da (Org.). Biological Sciences Foudantions. 1. ed. Ponta Grossa, PR: Atena Editora, 2019. p. 93-100
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/handle/123456789/32804
dc.identifier.isbn.none.fl_str_mv 978-85-7247-173-2
dc.identifier.doi.none.fl_str_mv 10.22533/at.ed.732191303
identifier_str_mv VERAS, Bruno Oliveira de et al. Screening of L-Asparaginase the salt-tolerant and thermostable marine Bacillus Subtilis Strain SR61. In: LUZ, Patrícia Michele da (Org.). Biological Sciences Foudantions. 1. ed. Ponta Grossa, PR: Atena Editora, 2019. p. 93-100
978-85-7247-173-2
10.22533/at.ed.732191303
url https://repositorio.ufrn.br/handle/123456789/32804
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Atena Editora
publisher.none.fl_str_mv Atena Editora
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFRN
instname:Universidade Federal do Rio Grande do Norte (UFRN)
instacron:UFRN
instname_str Universidade Federal do Rio Grande do Norte (UFRN)
instacron_str UFRN
institution UFRN
reponame_str Repositório Institucional da UFRN
collection Repositório Institucional da UFRN
bitstream.url.fl_str_mv https://repositorio.ufrn.br/bitstream/123456789/32804/1/ScreeningLAsparaginaseBacillus_Franca_2019.pdf
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