Screening of L-Asparaginase the salt-tolerant and thermostable marine Bacillus Subtilis Strain SR61

• Autor(a) principal: Veras, Bruno Oliveira de
• Data de Publicação: 2019
• Outros Autores: Santos, Yago Queiroz dos, França, Anderson Felipe Jácome de, Ribeiro, Penha Patricia Cabral, Barbosa, Elaine Costa Almeida, Gorlach-Lira, Krystyna
• Tipo de documento: Capítulo de livro
• Idioma: eng
• Título da fonte: Repositório Institucional da UFRN
• Texto Completo: https://repositorio.ufrn.br/handle/123456789/32804
• topic: L-Asparaginase; Bactéria; Enzyme activity; Bactérias; Atividade enzimática

The marine environment harbors different microorganisms that inhabit niches with adverse conditions, such as variation of temperature, pressure and salinity. To survive these particular conditions, marine bacteria use unique metabolic and biochemical characteristics, producing enzymes that have high industrial value. The objective of this study was to observe the production of thermostable and halotolerant L-asparaginase by marine bacteria isolated from the coral reefs of Cabo Branco, State of Paraíba, Brazil. An L-asparaginase-producing bacterial isolate was identified and identified by phylogenetic analysis as Bacillus subtilis SR61, via a 16S ribosomal RNA assay. For screening of L-asparaginase by SR61, it was inoculated in a differential medium to induce the production of salt tolerant extracellular thermostable enzyme with the addition of increasing salt concentrations (0, 0.5, 1.0, 1.5 e 2.0 M NaCl) by 55 0C for 24 hours. The screening showed a capacity of halotolerant and thermostable L-asparaginase production by the isolate identified as Bacillus subtilis, the production being limited to 1.0 M salt, having a total activity (IU / mL) 231.4 ± 3.57 and specifying IU / μg 8.39. Bacillus subtilis SR61 was able to produce L-asparaginase when submitted to a high salinity environment, demonstrating the halophytic nature of the isolate, having several applications in several industrial branches