Anti-IIa activity and antitumor properties of a hybrid heparin/heparan sulfate-like compound from Litopenaeus vannamei shrimp
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/handle/123456789/30995 |
Resumo: | In this present study, the anti-IIa activity and the antitumor properties of a hybrid heparin/heparan sulfate-like compound (sH/HS) from Litopenaeus vannamei shrimp heads are related. In addition to inhibiting 90.7% of thrombin activity at the lowest tested concentration (0.5 μg/mL), sH/HS compound stimulated the synthesis of antithrombotic heparan sulfate by endothelial cells in a dose-dependent manner. In vitro experiments demonstrated that the molecule from shrimp displayed a potent anti-angiogenic effect, reducing over 80% of the tubular structures formation at 50 and 100 μg/mL. In addition, sH/HS compound was able to inhibit the migration of B16F10 cells at all tested concentrations without affecting the cell viability. Although the studied compound had no effect on the proliferation of such cells during a period of 24 h, it had a significant long-term antiproliferative effect, reducing about 80% of colony formation and anchorage-independent growth at 50 and 100 μg/mL concentrations. When its effectiveness was tested in vivo, it was demonstrated that sH/HS promoted a reduction of more than 90% of tumor growth. In the context of thromboembolic disorders associated with cancer, such findings make the sH/HS compound an excellent target for studies on inhibiting of development and tumor progression, and the prevention of coagulopathies |
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Brito, Adriana da SilvaCavalcante, Rômulo S.Cavalheiro, Renan P.Palhares, Laís C. G. F.Nobre, Leonardo Thiago Duarte BarretoSouza, Giulianna Paiva Viana de AndradeNader, Helena B.Lima, Marcelo A.Chavante, Suely Ferreira2020-12-15T14:44:31Z2020-12-15T14:44:31Z2018-06-30BRITO, Adriana da Silva; CAVALCANTE, Rômulo S.; CAVALHEIRO, Renan P.; PALHARES, Laís C. G. F.; NOBRE, Leonardo Thiago Duarte Barreto; SOUZA, Giulianna Paiva Viana de Andrade; NADER, Helena B.; LIMA, Marcelo A.; CHAVANTE, Suely Ferreira Anti-IIa activity and antitumor properties of a hybrid heparin/heparan sulfate-like compound from Litopenaeus vannamei shrimp. International Journal Of Biological Macromolecules, [s. l.], v. 118, p. 1470-1478, out. 2018. Elsevier BV. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S0141813018311577?via%3Dihub#!. Acesso em: 21 set. 2020. http://dx.doi.org/10.1016/j.ijbiomac.2018.06.1430141-8130 (print)https://repositorio.ufrn.br/handle/123456789/3099510.1016/j.ijbiomac.2018.06.143ElsevierMarine invertebrateSulfated polysaccharideCancerAnti-IIa activity and antitumor properties of a hybrid heparin/heparan sulfate-like compound from Litopenaeus vannamei shrimpinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleIn this present study, the anti-IIa activity and the antitumor properties of a hybrid heparin/heparan sulfate-like compound (sH/HS) from Litopenaeus vannamei shrimp heads are related. In addition to inhibiting 90.7% of thrombin activity at the lowest tested concentration (0.5 μg/mL), sH/HS compound stimulated the synthesis of antithrombotic heparan sulfate by endothelial cells in a dose-dependent manner. In vitro experiments demonstrated that the molecule from shrimp displayed a potent anti-angiogenic effect, reducing over 80% of the tubular structures formation at 50 and 100 μg/mL. In addition, sH/HS compound was able to inhibit the migration of B16F10 cells at all tested concentrations without affecting the cell viability. Although the studied compound had no effect on the proliferation of such cells during a period of 24 h, it had a significant long-term antiproliferative effect, reducing about 80% of colony formation and anchorage-independent growth at 50 and 100 μg/mL concentrations. When its effectiveness was tested in vivo, it was demonstrated that sH/HS promoted a reduction of more than 90% of tumor growth. In the context of thromboembolic disorders associated with cancer, such findings make the sH/HS compound an excellent target for studies on inhibiting of development and tumor progression, and the prevention of coagulopathiesengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNinfo:eu-repo/semantics/openAccessCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.ufrn.br/bitstream/123456789/30995/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/30995/3/license.txte9597aa2854d128fd968be5edc8a28d9MD53TEXTAnti-IIaActivityAntitumor_Nobre_2018.pdf.txtAnti-IIaActivityAntitumor_Nobre_2018.pdf.txtExtracted texttext/plain49758https://repositorio.ufrn.br/bitstream/123456789/30995/4/Anti-IIaActivityAntitumor_Nobre_2018.pdf.txtb198b04ea29139ff15c66341b2db4054MD54THUMBNAILAnti-IIaActivityAntitumor_Nobre_2018.pdf.jpgAnti-IIaActivityAntitumor_Nobre_2018.pdf.jpgGenerated Thumbnailimage/jpeg1666https://repositorio.ufrn.br/bitstream/123456789/30995/5/Anti-IIaActivityAntitumor_Nobre_2018.pdf.jpgba2945de76d54815aa94f3636f97712fMD55123456789/309952023-02-07 16:37:18.037oai:https://repositorio.ufrn.br:123456789/30995Tk9OLUVYQ0xVU0lWRSBESVNUUklCVVRJT04gTElDRU5TRQoKCkJ5IHNpZ25pbmcgYW5kIGRlbGl2ZXJpbmcgdGhpcyBsaWNlbnNlLCBNci4gKGF1dGhvciBvciBjb3B5cmlnaHQgaG9sZGVyKToKCgphKSBHcmFudHMgdGhlIFVuaXZlcnNpZGFkZSBGZWRlcmFsIFJpbyBHcmFuZGUgZG8gTm9ydGUgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgb2YKcmVwcm9kdWNlLCBjb252ZXJ0IChhcyBkZWZpbmVkIGJlbG93KSwgY29tbXVuaWNhdGUgYW5kIC8gb3IKZGlzdHJpYnV0ZSB0aGUgZGVsaXZlcmVkIGRvY3VtZW50IChpbmNsdWRpbmcgYWJzdHJhY3QgLyBhYnN0cmFjdCkgaW4KZGlnaXRhbCBvciBwcmludGVkIGZvcm1hdCBhbmQgaW4gYW55IG1lZGl1bS4KCmIpIERlY2xhcmVzIHRoYXQgdGhlIGRvY3VtZW50IHN1Ym1pdHRlZCBpcyBpdHMgb3JpZ2luYWwgd29yaywgYW5kIHRoYXQKeW91IGhhdmUgdGhlIHJpZ2h0IHRvIGdyYW50IHRoZSByaWdodHMgY29udGFpbmVkIGluIHRoaXMgbGljZW5zZS4gRGVjbGFyZXMKdGhhdCB0aGUgZGVsaXZlcnkgb2YgdGhlIGRvY3VtZW50IGRvZXMgbm90IGluZnJpbmdlLCBhcyBmYXIgYXMgaXQgaXMKdGhlIHJpZ2h0cyBvZiBhbnkgb3RoZXIgcGVyc29uIG9yIGVudGl0eS4KCmMpIElmIHRoZSBkb2N1bWVudCBkZWxpdmVyZWQgY29udGFpbnMgbWF0ZXJpYWwgd2hpY2ggZG9lcyBub3QKcmlnaHRzLCBkZWNsYXJlcyB0aGF0IGl0IGhhcyBvYnRhaW5lZCBhdXRob3JpemF0aW9uIGZyb20gdGhlIGhvbGRlciBvZiB0aGUKY29weXJpZ2h0IHRvIGdyYW50IHRoZSBVbml2ZXJzaWRhZGUgRmVkZXJhbCBkbyBSaW8gR3JhbmRlIGRvIE5vcnRlIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdCB0aGlzIG1hdGVyaWFsIHdob3NlIHJpZ2h0cyBhcmUgb2YKdGhpcmQgcGFydGllcyBpcyBjbGVhcmx5IGlkZW50aWZpZWQgYW5kIHJlY29nbml6ZWQgaW4gdGhlIHRleHQgb3IKY29udGVudCBvZiB0aGUgZG9jdW1lbnQgZGVsaXZlcmVkLgoKSWYgdGhlIGRvY3VtZW50IHN1Ym1pdHRlZCBpcyBiYXNlZCBvbiBmdW5kZWQgb3Igc3VwcG9ydGVkIHdvcmsKYnkgYW5vdGhlciBpbnN0aXR1dGlvbiBvdGhlciB0aGFuIHRoZSBVbml2ZXJzaWRhZGUgRmVkZXJhbCBkbyBSaW8gR3JhbmRlIGRvIE5vcnRlLCBkZWNsYXJlcyB0aGF0IGl0IGhhcyBmdWxmaWxsZWQgYW55IG9ibGlnYXRpb25zIHJlcXVpcmVkIGJ5IHRoZSByZXNwZWN0aXZlIGFncmVlbWVudCBvciBhZ3JlZW1lbnQuCgpUaGUgVW5pdmVyc2lkYWRlIEZlZGVyYWwgZG8gUmlvIEdyYW5kZSBkbyBOb3J0ZSB3aWxsIGNsZWFybHkgaWRlbnRpZnkgaXRzIG5hbWUgKHMpIGFzIHRoZSBhdXRob3IgKHMpIG9yIGhvbGRlciAocykgb2YgdGhlIGRvY3VtZW50J3MgcmlnaHRzCmRlbGl2ZXJlZCwgYW5kIHdpbGwgbm90IG1ha2UgYW55IGNoYW5nZXMsIG90aGVyIHRoYW4gdGhvc2UgcGVybWl0dGVkIGJ5CnRoaXMgbGljZW5zZQo=Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2023-02-07T19:37:18Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
Anti-IIa activity and antitumor properties of a hybrid heparin/heparan sulfate-like compound from Litopenaeus vannamei shrimp |
title |
Anti-IIa activity and antitumor properties of a hybrid heparin/heparan sulfate-like compound from Litopenaeus vannamei shrimp |
spellingShingle |
Anti-IIa activity and antitumor properties of a hybrid heparin/heparan sulfate-like compound from Litopenaeus vannamei shrimp Brito, Adriana da Silva Marine invertebrate Sulfated polysaccharide Cancer |
title_short |
Anti-IIa activity and antitumor properties of a hybrid heparin/heparan sulfate-like compound from Litopenaeus vannamei shrimp |
title_full |
Anti-IIa activity and antitumor properties of a hybrid heparin/heparan sulfate-like compound from Litopenaeus vannamei shrimp |
title_fullStr |
Anti-IIa activity and antitumor properties of a hybrid heparin/heparan sulfate-like compound from Litopenaeus vannamei shrimp |
title_full_unstemmed |
Anti-IIa activity and antitumor properties of a hybrid heparin/heparan sulfate-like compound from Litopenaeus vannamei shrimp |
title_sort |
Anti-IIa activity and antitumor properties of a hybrid heparin/heparan sulfate-like compound from Litopenaeus vannamei shrimp |
author |
Brito, Adriana da Silva |
author_facet |
Brito, Adriana da Silva Cavalcante, Rômulo S. Cavalheiro, Renan P. Palhares, Laís C. G. F. Nobre, Leonardo Thiago Duarte Barreto Souza, Giulianna Paiva Viana de Andrade Nader, Helena B. Lima, Marcelo A. Chavante, Suely Ferreira |
author_role |
author |
author2 |
Cavalcante, Rômulo S. Cavalheiro, Renan P. Palhares, Laís C. G. F. Nobre, Leonardo Thiago Duarte Barreto Souza, Giulianna Paiva Viana de Andrade Nader, Helena B. Lima, Marcelo A. Chavante, Suely Ferreira |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Brito, Adriana da Silva Cavalcante, Rômulo S. Cavalheiro, Renan P. Palhares, Laís C. G. F. Nobre, Leonardo Thiago Duarte Barreto Souza, Giulianna Paiva Viana de Andrade Nader, Helena B. Lima, Marcelo A. Chavante, Suely Ferreira |
dc.subject.por.fl_str_mv |
Marine invertebrate Sulfated polysaccharide Cancer |
topic |
Marine invertebrate Sulfated polysaccharide Cancer |
description |
In this present study, the anti-IIa activity and the antitumor properties of a hybrid heparin/heparan sulfate-like compound (sH/HS) from Litopenaeus vannamei shrimp heads are related. In addition to inhibiting 90.7% of thrombin activity at the lowest tested concentration (0.5 μg/mL), sH/HS compound stimulated the synthesis of antithrombotic heparan sulfate by endothelial cells in a dose-dependent manner. In vitro experiments demonstrated that the molecule from shrimp displayed a potent anti-angiogenic effect, reducing over 80% of the tubular structures formation at 50 and 100 μg/mL. In addition, sH/HS compound was able to inhibit the migration of B16F10 cells at all tested concentrations without affecting the cell viability. Although the studied compound had no effect on the proliferation of such cells during a period of 24 h, it had a significant long-term antiproliferative effect, reducing about 80% of colony formation and anchorage-independent growth at 50 and 100 μg/mL concentrations. When its effectiveness was tested in vivo, it was demonstrated that sH/HS promoted a reduction of more than 90% of tumor growth. In the context of thromboembolic disorders associated with cancer, such findings make the sH/HS compound an excellent target for studies on inhibiting of development and tumor progression, and the prevention of coagulopathies |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018-06-30 |
dc.date.accessioned.fl_str_mv |
2020-12-15T14:44:31Z |
dc.date.available.fl_str_mv |
2020-12-15T14:44:31Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
BRITO, Adriana da Silva; CAVALCANTE, Rômulo S.; CAVALHEIRO, Renan P.; PALHARES, Laís C. G. F.; NOBRE, Leonardo Thiago Duarte Barreto; SOUZA, Giulianna Paiva Viana de Andrade; NADER, Helena B.; LIMA, Marcelo A.; CHAVANTE, Suely Ferreira Anti-IIa activity and antitumor properties of a hybrid heparin/heparan sulfate-like compound from Litopenaeus vannamei shrimp. International Journal Of Biological Macromolecules, [s. l.], v. 118, p. 1470-1478, out. 2018. Elsevier BV. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S0141813018311577?via%3Dihub#!. Acesso em: 21 set. 2020. http://dx.doi.org/10.1016/j.ijbiomac.2018.06.143 |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/handle/123456789/30995 |
dc.identifier.issn.none.fl_str_mv |
0141-8130 (print) |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.ijbiomac.2018.06.143 |
identifier_str_mv |
BRITO, Adriana da Silva; CAVALCANTE, Rômulo S.; CAVALHEIRO, Renan P.; PALHARES, Laís C. G. F.; NOBRE, Leonardo Thiago Duarte Barreto; SOUZA, Giulianna Paiva Viana de Andrade; NADER, Helena B.; LIMA, Marcelo A.; CHAVANTE, Suely Ferreira Anti-IIa activity and antitumor properties of a hybrid heparin/heparan sulfate-like compound from Litopenaeus vannamei shrimp. International Journal Of Biological Macromolecules, [s. l.], v. 118, p. 1470-1478, out. 2018. Elsevier BV. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S0141813018311577?via%3Dihub#!. Acesso em: 21 set. 2020. http://dx.doi.org/10.1016/j.ijbiomac.2018.06.143 0141-8130 (print) 10.1016/j.ijbiomac.2018.06.143 |
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eng |
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Elsevier |
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Elsevier |
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