Optimization of separation of bioactive compounds in daily foods by aqueous two-phase flotation coupled with HPLC-DAD

Detalhes bibliográficos
Autor(a) principal: Santos, Everaldo Silvino dos
Data de Publicação: 2017
Outros Autores: Padilha, Carlos Eduardo de Araújo, Nogueira, Cleitiane da Costa, Leitão, Ana Laura de Sá, Rocha, Patrícia Maria, Almeida, Hanna Nóbrega, Souza, Domingos Fabiano Santana, Oliveira, Jackson Araújo de, Macedo, Gorete Ribeiro de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/handle/123456789/32317
Resumo: In this study, we have used Aqueous Two-Phase Flotation (ATPF) in order to recovery and concentrate bioactive phenolic compounds found in foods. Firstly, the effects of the operational conditions on the ATPF performance have been investigated using a model solution of Gallic Acid (GA). The optimal conditions were: [ammonium sulfate] = 450 g/L, pH = 3.8, air flowrate = 37.5 mL/min, time flotation = 120 min, initial organic phase volume = 10.0 mL and polyethylene glycol 400 as solvent. Next, experiments were carried out with aqueous extracts of daily foods and the ATPF phases were analyzed by HPLC-DAD. In terms of total phenolic compounds content, the highest percentage of separation was obtained for clove extract with 38.80% due to transfer of gallic acid and eugenol to PEG rich-phase. The results showed that ATPF is a promising technique for be applied for separation and purification of phenolic compounds mainly in natural extracts
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spelling Santos, Everaldo Silvino dosPadilha, Carlos Eduardo de AraújoNogueira, Cleitiane da CostaLeitão, Ana Laura de SáRocha, Patrícia MariaAlmeida, Hanna NóbregaSouza, Domingos Fabiano SantanaOliveira, Jackson Araújo deMacedo, Gorete Ribeiro de2021-04-26T14:43:01Z2021-04-26T14:43:01Z2017-06-10PADILHA, C. E.; NOGUEIRA, C. C.; LEITAO, A. L. S.; ROCHA, P. M.; ALMEIDA, H. N.; SOUZA, D. S. S.; OLIVEIRA, J. A.; MACEDO, G. R.; SANTOS, E. S.. Optimization of Separation of Bioactive Compounds in Daily Foods by Aqueous Two-Phase Flotation Coupled With HPLC-DADOptimization of Separation of Bioactive Compounds in Daily Foods by Aqueous Two-Phase Flotation Coupled With HPLC-DAD. International Journal of Chromatography and Separation Techniques, v. 106, p. 1-8, 2017. Disponível em https://gavinpublishers.com/articles/research-article/International-Journal-of-Chromatography-and-Separation-Techniques/optimization-of-separation-of-bioactive-compounds-in-daily-foods-by-aqueous-two-phase-flotation-coupled-with-hplc-dad. Acesso em: 25 mar. 2021. http://www.doi.org/10.29011/IJCST-106.000006.2577-218Xhttps://repositorio.ufrn.br/handle/123456789/3231710.29011/IJCST-106.000006Gavin PublishersAttribution-ShareAlike 3.0 Brazilhttp://creativecommons.org/licenses/by-sa/3.0/br/info:eu-repo/semantics/openAccessAqueous Two-Phase FlotationConcentrationFoodsGallic AcidPolyethylene GlycolRecoveryOptimization of separation of bioactive compounds in daily foods by aqueous two-phase flotation coupled with HPLC-DADinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleIn this study, we have used Aqueous Two-Phase Flotation (ATPF) in order to recovery and concentrate bioactive phenolic compounds found in foods. Firstly, the effects of the operational conditions on the ATPF performance have been investigated using a model solution of Gallic Acid (GA). The optimal conditions were: [ammonium sulfate] = 450 g/L, pH = 3.8, air flowrate = 37.5 mL/min, time flotation = 120 min, initial organic phase volume = 10.0 mL and polyethylene glycol 400 as solvent. Next, experiments were carried out with aqueous extracts of daily foods and the ATPF phases were analyzed by HPLC-DAD. In terms of total phenolic compounds content, the highest percentage of separation was obtained for clove extract with 38.80% due to transfer of gallic acid and eugenol to PEG rich-phase. The results showed that ATPF is a promising technique for be applied for separation and purification of phenolic compounds mainly in natural extractsengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALOptimizationSeparationBioactive_Santos_2017.pdfOptimizationSeparationBioactive_Santos_2017.pdfapplication/pdf881544https://repositorio.ufrn.br/bitstream/123456789/32317/1/OptimizationSeparationBioactive_Santos_2017.pdf0f58478ab98a948a6dd2da1458f53583MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-81031https://repositorio.ufrn.br/bitstream/123456789/32317/2/license_rdf9b85e4235558a2887c2be3998124b615MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/32317/3/license.txte9597aa2854d128fd968be5edc8a28d9MD53TEXTOptimizationSeparationBioactive_Santos_2017.pdf.txtOptimizationSeparationBioactive_Santos_2017.pdf.txtExtracted texttext/plain32024https://repositorio.ufrn.br/bitstream/123456789/32317/4/OptimizationSeparationBioactive_Santos_2017.pdf.txtb35399c06b765d9a16f5fef308d1e6e8MD54THUMBNAILOptimizationSeparationBioactive_Santos_2017.pdf.jpgOptimizationSeparationBioactive_Santos_2017.pdf.jpgGenerated Thumbnailimage/jpeg1807https://repositorio.ufrn.br/bitstream/123456789/32317/5/OptimizationSeparationBioactive_Santos_2017.pdf.jpg6c0e373d8730bd87c4fe48dbf05f5d2aMD55123456789/323172021-05-02 08:35:33.898oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2021-05-02T11:35:33Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.pt_BR.fl_str_mv Optimization of separation of bioactive compounds in daily foods by aqueous two-phase flotation coupled with HPLC-DAD
title Optimization of separation of bioactive compounds in daily foods by aqueous two-phase flotation coupled with HPLC-DAD
spellingShingle Optimization of separation of bioactive compounds in daily foods by aqueous two-phase flotation coupled with HPLC-DAD
Santos, Everaldo Silvino dos
Aqueous Two-Phase Flotation
Concentration
Foods
Gallic Acid
Polyethylene Glycol
Recovery
title_short Optimization of separation of bioactive compounds in daily foods by aqueous two-phase flotation coupled with HPLC-DAD
title_full Optimization of separation of bioactive compounds in daily foods by aqueous two-phase flotation coupled with HPLC-DAD
title_fullStr Optimization of separation of bioactive compounds in daily foods by aqueous two-phase flotation coupled with HPLC-DAD
title_full_unstemmed Optimization of separation of bioactive compounds in daily foods by aqueous two-phase flotation coupled with HPLC-DAD
title_sort Optimization of separation of bioactive compounds in daily foods by aqueous two-phase flotation coupled with HPLC-DAD
author Santos, Everaldo Silvino dos
author_facet Santos, Everaldo Silvino dos
Padilha, Carlos Eduardo de Araújo
Nogueira, Cleitiane da Costa
Leitão, Ana Laura de Sá
Rocha, Patrícia Maria
Almeida, Hanna Nóbrega
Souza, Domingos Fabiano Santana
Oliveira, Jackson Araújo de
Macedo, Gorete Ribeiro de
author_role author
author2 Padilha, Carlos Eduardo de Araújo
Nogueira, Cleitiane da Costa
Leitão, Ana Laura de Sá
Rocha, Patrícia Maria
Almeida, Hanna Nóbrega
Souza, Domingos Fabiano Santana
Oliveira, Jackson Araújo de
Macedo, Gorete Ribeiro de
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, Everaldo Silvino dos
Padilha, Carlos Eduardo de Araújo
Nogueira, Cleitiane da Costa
Leitão, Ana Laura de Sá
Rocha, Patrícia Maria
Almeida, Hanna Nóbrega
Souza, Domingos Fabiano Santana
Oliveira, Jackson Araújo de
Macedo, Gorete Ribeiro de
dc.subject.por.fl_str_mv Aqueous Two-Phase Flotation
Concentration
Foods
Gallic Acid
Polyethylene Glycol
Recovery
topic Aqueous Two-Phase Flotation
Concentration
Foods
Gallic Acid
Polyethylene Glycol
Recovery
description In this study, we have used Aqueous Two-Phase Flotation (ATPF) in order to recovery and concentrate bioactive phenolic compounds found in foods. Firstly, the effects of the operational conditions on the ATPF performance have been investigated using a model solution of Gallic Acid (GA). The optimal conditions were: [ammonium sulfate] = 450 g/L, pH = 3.8, air flowrate = 37.5 mL/min, time flotation = 120 min, initial organic phase volume = 10.0 mL and polyethylene glycol 400 as solvent. Next, experiments were carried out with aqueous extracts of daily foods and the ATPF phases were analyzed by HPLC-DAD. In terms of total phenolic compounds content, the highest percentage of separation was obtained for clove extract with 38.80% due to transfer of gallic acid and eugenol to PEG rich-phase. The results showed that ATPF is a promising technique for be applied for separation and purification of phenolic compounds mainly in natural extracts
publishDate 2017
dc.date.issued.fl_str_mv 2017-06-10
dc.date.accessioned.fl_str_mv 2021-04-26T14:43:01Z
dc.date.available.fl_str_mv 2021-04-26T14:43:01Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PADILHA, C. E.; NOGUEIRA, C. C.; LEITAO, A. L. S.; ROCHA, P. M.; ALMEIDA, H. N.; SOUZA, D. S. S.; OLIVEIRA, J. A.; MACEDO, G. R.; SANTOS, E. S.. Optimization of Separation of Bioactive Compounds in Daily Foods by Aqueous Two-Phase Flotation Coupled With HPLC-DADOptimization of Separation of Bioactive Compounds in Daily Foods by Aqueous Two-Phase Flotation Coupled With HPLC-DAD. International Journal of Chromatography and Separation Techniques, v. 106, p. 1-8, 2017. Disponível em https://gavinpublishers.com/articles/research-article/International-Journal-of-Chromatography-and-Separation-Techniques/optimization-of-separation-of-bioactive-compounds-in-daily-foods-by-aqueous-two-phase-flotation-coupled-with-hplc-dad. Acesso em: 25 mar. 2021. http://www.doi.org/10.29011/IJCST-106.000006.
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/handle/123456789/32317
dc.identifier.issn.none.fl_str_mv 2577-218X
dc.identifier.doi.none.fl_str_mv 10.29011/IJCST-106.000006
identifier_str_mv PADILHA, C. E.; NOGUEIRA, C. C.; LEITAO, A. L. S.; ROCHA, P. M.; ALMEIDA, H. N.; SOUZA, D. S. S.; OLIVEIRA, J. A.; MACEDO, G. R.; SANTOS, E. S.. Optimization of Separation of Bioactive Compounds in Daily Foods by Aqueous Two-Phase Flotation Coupled With HPLC-DADOptimization of Separation of Bioactive Compounds in Daily Foods by Aqueous Two-Phase Flotation Coupled With HPLC-DAD. International Journal of Chromatography and Separation Techniques, v. 106, p. 1-8, 2017. Disponível em https://gavinpublishers.com/articles/research-article/International-Journal-of-Chromatography-and-Separation-Techniques/optimization-of-separation-of-bioactive-compounds-in-daily-foods-by-aqueous-two-phase-flotation-coupled-with-hplc-dad. Acesso em: 25 mar. 2021. http://www.doi.org/10.29011/IJCST-106.000006.
2577-218X
10.29011/IJCST-106.000006
url https://repositorio.ufrn.br/handle/123456789/32317
dc.language.iso.fl_str_mv eng
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dc.rights.driver.fl_str_mv Attribution-ShareAlike 3.0 Brazil
http://creativecommons.org/licenses/by-sa/3.0/br/
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rights_invalid_str_mv Attribution-ShareAlike 3.0 Brazil
http://creativecommons.org/licenses/by-sa/3.0/br/
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dc.publisher.none.fl_str_mv Gavin Publishers
publisher.none.fl_str_mv Gavin Publishers
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