Expressão imuno-histoquimica da cicloxigenase-2 e p53 em cancinoma epidermóide oral
Autor(a) principal: | |
---|---|
Data de Publicação: | 2006 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/jspui/handle/123456789/17137 |
Resumo: | Squamous cell carcinoma is the most common malignant neoplasm in the oral cavity, accounting for more than 90% of all malignancies in this location. Cyclooxygenases (COX s) are key enzymes on arachidonic acid metabolism and prostaglandin synthesis, being expressed basically in two forms: the constitutive (COX-1) and the inducible (COX-2). Increased levels on the expression of COX-2 have been implicated in the pathogenesis tumor progression of various forms of human cancer, including oral squamous cell carcinoma, some of what suggesting a possible interaction between COX-2 and the protein expressed by the tumor suppressor gene p53, mutated in more than 50% of all human cancers. The mean of the present research consisted in analyze the correlation between the expression of COX-2 and p53, at the protein level, as well as evaluate the difference on the expression of these two proteins with the histological grading of malignancy. 34 cases of oral squamous cell carcinoma were selected and graded according to the histological grading system proposed by Bryne (1998) and the labeling indexes (LI s) for COX-2 and p53 evaluated using immunohistochemistry method. The results revealed that COX-2 was expressed in increased levels in most of the specimens, although there was no statistic significant correlation between LI s from COX-2 and p53 (p>0.05), and there were no statistical differences on the expression of these proteins between tumors of high and low grade of malignancy (p>0.05). Interestingly, the expression of COX-2 and p53 was detected in fragments of dysplastic oral epithelium adjacent to tumor areas, on basal and suprabasal layers. The absence of statistical correlation between the expression of COX-2 and p53 proteins do not rule ot the existence of a relation between them, were it may reflect the diversity of regulatory pathways between both, different direct and indirect inhibitory effects of COX-2 over p53, as well as the wide range of activation macheenisms for COX-2 and mutational status of the p53 gene Another conclusion point that the increased expression of COX-2 observed in oral squamous cell carcinomas suggest a role for this protein in the processes of pathogenesis and tumoral evolution of this malignant neoplasm |
id |
UFRN_e712d6c7426783adc0ee8c5447a76f73 |
---|---|
oai_identifier_str |
oai:https://repositorio.ufrn.br:123456789/17137 |
network_acronym_str |
UFRN |
network_name_str |
Repositório Institucional da UFRN |
repository_id_str |
|
spelling |
Goulart Filho, João Augusto Viannahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4125022J8&dataRevisao=nullhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4794937Z9Bento, Patrícia Meirahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4776885U7&dataRevisao=nullFreitas, Roseana de Almeidahttp://lattes.cnpq.br/9512014003639405Galvão, Hébel Cavalcanti2014-12-17T15:32:24Z2007-07-132014-12-17T15:32:24Z2006-02-17GOULART FILHO, João Augusto Vianna. Expressão imuno-histoquimica da cicloxigenase-2 e p53 em cancinoma epidermóide oral. 2006. 145 f. Dissertação (Mestrado em Odontologia) - Universidade Federal do Rio Grande do Norte, Natal, 2006.https://repositorio.ufrn.br/jspui/handle/123456789/17137Squamous cell carcinoma is the most common malignant neoplasm in the oral cavity, accounting for more than 90% of all malignancies in this location. Cyclooxygenases (COX s) are key enzymes on arachidonic acid metabolism and prostaglandin synthesis, being expressed basically in two forms: the constitutive (COX-1) and the inducible (COX-2). Increased levels on the expression of COX-2 have been implicated in the pathogenesis tumor progression of various forms of human cancer, including oral squamous cell carcinoma, some of what suggesting a possible interaction between COX-2 and the protein expressed by the tumor suppressor gene p53, mutated in more than 50% of all human cancers. The mean of the present research consisted in analyze the correlation between the expression of COX-2 and p53, at the protein level, as well as evaluate the difference on the expression of these two proteins with the histological grading of malignancy. 34 cases of oral squamous cell carcinoma were selected and graded according to the histological grading system proposed by Bryne (1998) and the labeling indexes (LI s) for COX-2 and p53 evaluated using immunohistochemistry method. The results revealed that COX-2 was expressed in increased levels in most of the specimens, although there was no statistic significant correlation between LI s from COX-2 and p53 (p>0.05), and there were no statistical differences on the expression of these proteins between tumors of high and low grade of malignancy (p>0.05). Interestingly, the expression of COX-2 and p53 was detected in fragments of dysplastic oral epithelium adjacent to tumor areas, on basal and suprabasal layers. The absence of statistical correlation between the expression of COX-2 and p53 proteins do not rule ot the existence of a relation between them, were it may reflect the diversity of regulatory pathways between both, different direct and indirect inhibitory effects of COX-2 over p53, as well as the wide range of activation macheenisms for COX-2 and mutational status of the p53 gene Another conclusion point that the increased expression of COX-2 observed in oral squamous cell carcinomas suggest a role for this protein in the processes of pathogenesis and tumoral evolution of this malignant neoplasmO carcinoma epidermóide é a neoplasia maligna mais comum na cavidade oral, representando mais de 90% das malignidades nesta localização. As cicloxigenases (COX s) são enzimas chave no metabolismo do ácido aracdônico e síntese de prostaglandinas, sendo expressas basicamente sob duas formas: uma constitutiva (COX-1) e uma induzida (COX-2). Níveis elevados na expressão da COX-2 têm sido implicados na patogênese e progressão tumoral em diversos tipos de câncer em humanos, incluindo o carcinoma epidermóide oral, alguns dos quais sugerindo uma possível interação entre a COX-2 e a proteína expressa pelo gene supressor tumoral p53, mutado em mais de 50% de todos cânceres humanos. O propósito da presente pesquisa consistiu em analisar a correlação entre a expressão de COX-2 e p53, em nível de proteína, bem como avaliar a diferença na expressão destas duas proteínas em relação ao grau histológico de malignidade. Para tal, foram selecionados 34 casos de carcinoma epidermóide oral, os quais foram classificados de acordo com o sistema de gradação histológica de malignidade proposto por Bryne (1998) e cujos índices de positividade para COX-2 e p53 foram avaliados através da técnica imuno-histoquímica. O resultados revelaram que a COX-2 esteve expressa em níveis elevados na maior parte dos espécimes analisados, embora não se tenha verificado correlação estatisticamente significativa entre os IP s da COX-2 e da p53 (p>0,05), tampouco diferença estatisticamente significativa entre a expressão destas proteínas entre tumores de alto e baixo grau de malignidade (p>0,05). Interessantemente, foi detectada a expressão da COX-2 e da p53 em fragmentos de epitélio oral displásico, nas camadas basal e parabasal, adjacentes ao tumor. A ausência de correlação estatística entre a expressão das proteínas COX-2 e p53 não descarta a existência de uma relação entre as mesmas, podendo refletir a diversidade de vias regulatórias entre ambas, os diferentes efeitos inibitórios diretos e indiretos da COX-2 sobre a p53, bem como os inúmeros mecanismos de ativação da COX-2 e o estado mutacional do gene p53. Conclui-se ainda que a elevada expressão da COX-2 observada em carcinomas epidermóides orais sugere um papel desta proteína dentro dos processos de patogênese e evolução tumoral desta neoplasia malignaCoordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal do Rio Grande do NortePrograma de Pós-Graduação em Patologia OralUFRNBROdontologiaCicloxigenase-2, p53, carcinoma epidermóide oral, gradação histológica de malignidade imuno-histoquímicaCyclooxigenase-2, p53, oral squamous cell carcinoma, histological grading of malignancy, immunohistochemistryCNPQ::CIENCIAS DA SAUDE::ODONTOLOGIAExpressão imuno-histoquimica da cicloxigenase-2 e p53 em cancinoma epidermóide oralinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALJoaoAVGF.pdfapplication/pdf1413564https://repositorio.ufrn.br/bitstream/123456789/17137/1/JoaoAVGF.pdff5d8ea2da8907cd551169a4091430007MD51TEXTJoaoAVGF.pdf.txtJoaoAVGF.pdf.txtExtracted texttext/plain201142https://repositorio.ufrn.br/bitstream/123456789/17137/6/JoaoAVGF.pdf.txtd22fe9db7435f6225ec550742573d5f3MD56THUMBNAILJoaoAVGF.pdf.jpgJoaoAVGF.pdf.jpgIM Thumbnailimage/jpeg2040https://repositorio.ufrn.br/bitstream/123456789/17137/7/JoaoAVGF.pdf.jpg4b69dd634eeeeea33a131ede37803c83MD57123456789/171372017-11-04 13:35:40.958oai:https://repositorio.ufrn.br:123456789/17137Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2017-11-04T16:35:40Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.por.fl_str_mv |
Expressão imuno-histoquimica da cicloxigenase-2 e p53 em cancinoma epidermóide oral |
title |
Expressão imuno-histoquimica da cicloxigenase-2 e p53 em cancinoma epidermóide oral |
spellingShingle |
Expressão imuno-histoquimica da cicloxigenase-2 e p53 em cancinoma epidermóide oral Goulart Filho, João Augusto Vianna Cicloxigenase-2, p53, carcinoma epidermóide oral, gradação histológica de malignidade imuno-histoquímica Cyclooxigenase-2, p53, oral squamous cell carcinoma, histological grading of malignancy, immunohistochemistry CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
title_short |
Expressão imuno-histoquimica da cicloxigenase-2 e p53 em cancinoma epidermóide oral |
title_full |
Expressão imuno-histoquimica da cicloxigenase-2 e p53 em cancinoma epidermóide oral |
title_fullStr |
Expressão imuno-histoquimica da cicloxigenase-2 e p53 em cancinoma epidermóide oral |
title_full_unstemmed |
Expressão imuno-histoquimica da cicloxigenase-2 e p53 em cancinoma epidermóide oral |
title_sort |
Expressão imuno-histoquimica da cicloxigenase-2 e p53 em cancinoma epidermóide oral |
author |
Goulart Filho, João Augusto Vianna |
author_facet |
Goulart Filho, João Augusto Vianna |
author_role |
author |
dc.contributor.authorID.por.fl_str_mv |
|
dc.contributor.authorLattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4125022J8&dataRevisao=null |
dc.contributor.advisorID.por.fl_str_mv |
|
dc.contributor.advisorLattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4794937Z9 |
dc.contributor.referees1.pt_BR.fl_str_mv |
Bento, Patrícia Meira |
dc.contributor.referees1ID.por.fl_str_mv |
|
dc.contributor.referees1Lattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4776885U7&dataRevisao=null |
dc.contributor.referees2.pt_BR.fl_str_mv |
Freitas, Roseana de Almeida |
dc.contributor.referees2ID.por.fl_str_mv |
|
dc.contributor.referees2Lattes.por.fl_str_mv |
http://lattes.cnpq.br/9512014003639405 |
dc.contributor.author.fl_str_mv |
Goulart Filho, João Augusto Vianna |
dc.contributor.advisor1.fl_str_mv |
Galvão, Hébel Cavalcanti |
contributor_str_mv |
Galvão, Hébel Cavalcanti |
dc.subject.por.fl_str_mv |
Cicloxigenase-2, p53, carcinoma epidermóide oral, gradação histológica de malignidade imuno-histoquímica |
topic |
Cicloxigenase-2, p53, carcinoma epidermóide oral, gradação histológica de malignidade imuno-histoquímica Cyclooxigenase-2, p53, oral squamous cell carcinoma, histological grading of malignancy, immunohistochemistry CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
dc.subject.eng.fl_str_mv |
Cyclooxigenase-2, p53, oral squamous cell carcinoma, histological grading of malignancy, immunohistochemistry |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
description |
Squamous cell carcinoma is the most common malignant neoplasm in the oral cavity, accounting for more than 90% of all malignancies in this location. Cyclooxygenases (COX s) are key enzymes on arachidonic acid metabolism and prostaglandin synthesis, being expressed basically in two forms: the constitutive (COX-1) and the inducible (COX-2). Increased levels on the expression of COX-2 have been implicated in the pathogenesis tumor progression of various forms of human cancer, including oral squamous cell carcinoma, some of what suggesting a possible interaction between COX-2 and the protein expressed by the tumor suppressor gene p53, mutated in more than 50% of all human cancers. The mean of the present research consisted in analyze the correlation between the expression of COX-2 and p53, at the protein level, as well as evaluate the difference on the expression of these two proteins with the histological grading of malignancy. 34 cases of oral squamous cell carcinoma were selected and graded according to the histological grading system proposed by Bryne (1998) and the labeling indexes (LI s) for COX-2 and p53 evaluated using immunohistochemistry method. The results revealed that COX-2 was expressed in increased levels in most of the specimens, although there was no statistic significant correlation between LI s from COX-2 and p53 (p>0.05), and there were no statistical differences on the expression of these proteins between tumors of high and low grade of malignancy (p>0.05). Interestingly, the expression of COX-2 and p53 was detected in fragments of dysplastic oral epithelium adjacent to tumor areas, on basal and suprabasal layers. The absence of statistical correlation between the expression of COX-2 and p53 proteins do not rule ot the existence of a relation between them, were it may reflect the diversity of regulatory pathways between both, different direct and indirect inhibitory effects of COX-2 over p53, as well as the wide range of activation macheenisms for COX-2 and mutational status of the p53 gene Another conclusion point that the increased expression of COX-2 observed in oral squamous cell carcinomas suggest a role for this protein in the processes of pathogenesis and tumoral evolution of this malignant neoplasm |
publishDate |
2006 |
dc.date.issued.fl_str_mv |
2006-02-17 |
dc.date.available.fl_str_mv |
2007-07-13 2014-12-17T15:32:24Z |
dc.date.accessioned.fl_str_mv |
2014-12-17T15:32:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
GOULART FILHO, João Augusto Vianna. Expressão imuno-histoquimica da cicloxigenase-2 e p53 em cancinoma epidermóide oral. 2006. 145 f. Dissertação (Mestrado em Odontologia) - Universidade Federal do Rio Grande do Norte, Natal, 2006. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/jspui/handle/123456789/17137 |
identifier_str_mv |
GOULART FILHO, João Augusto Vianna. Expressão imuno-histoquimica da cicloxigenase-2 e p53 em cancinoma epidermóide oral. 2006. 145 f. Dissertação (Mestrado em Odontologia) - Universidade Federal do Rio Grande do Norte, Natal, 2006. |
url |
https://repositorio.ufrn.br/jspui/handle/123456789/17137 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal do Rio Grande do Norte |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Patologia Oral |
dc.publisher.initials.fl_str_mv |
UFRN |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Odontologia |
publisher.none.fl_str_mv |
Universidade Federal do Rio Grande do Norte |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFRN instname:Universidade Federal do Rio Grande do Norte (UFRN) instacron:UFRN |
instname_str |
Universidade Federal do Rio Grande do Norte (UFRN) |
instacron_str |
UFRN |
institution |
UFRN |
reponame_str |
Repositório Institucional da UFRN |
collection |
Repositório Institucional da UFRN |
bitstream.url.fl_str_mv |
https://repositorio.ufrn.br/bitstream/123456789/17137/1/JoaoAVGF.pdf https://repositorio.ufrn.br/bitstream/123456789/17137/6/JoaoAVGF.pdf.txt https://repositorio.ufrn.br/bitstream/123456789/17137/7/JoaoAVGF.pdf.jpg |
bitstream.checksum.fl_str_mv |
f5d8ea2da8907cd551169a4091430007 d22fe9db7435f6225ec550742573d5f3 4b69dd634eeeeea33a131ede37803c83 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN) |
repository.mail.fl_str_mv |
|
_version_ |
1802117735331659776 |