Effect of flow patterns on bovine serum albumin and ampicillin partitioning using aqueous two-phase systems in microdevice

Detalhes bibliográficos
Autor(a) principal: Santos, Everaldo Silvino dos
Data de Publicação: 2021
Outros Autores: Pedro, Marcelo da Silva, Oliveira, LuizAndré Fialho, Padilha, Carlos Eduardo de Araújo, Oliveira, Jackson Araújo de, Souza, Domingos Fabiano de Santana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/handle/123456789/32345
Resumo: 2026
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spelling Santos, Everaldo Silvino dosPedro, Marcelo da SilvaOliveira, LuizAndré FialhoPadilha, Carlos Eduardo de AraújoOliveira, Jackson Araújo deSouza, Domingos Fabiano de Santana2021-05-03T17:58:20Z2021-01-01PEDRO, Marcelo da Silva; OLIVEIRA, Luiz André Fialho; PADILHA, Carlos Eduardo de Araújo; SANTOS, Everaldo Silvino dos; OLIVEIRA, Jackson Araújo de; SOUZA, Domingos Fabiano de Santana. Effect of flow patterns on bovine serum albumin and ampicillin partitioning using aqueous two-phase systems in microdevice. Separation and Purification Technology, [S.L.], v. 254, p. 117592, jan. 2021. Disponível em https://www.sciencedirect.com/science/article/abs/pii/S1383586620320669?via%3Dihub. Acesso em 30 mar. 2021. https://doi.org/10.1016/j.seppur.2020.1175921383-5866https://repositorio.ufrn.br/handle/123456789/3234510.1016/j.seppur.2020.117592ElsevierMicrofluidExtractionPolyethylene glycolBovine serum albuminDroplet flow patternEffect of flow patterns on bovine serum albumin and ampicillin partitioning using aqueous two-phase systems in microdeviceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2026The separation and purification of biomolecules via aqueous two-phase extraction (ATPE) has encountered a series of obstacles to its industrial implementation. Recently, the miniaturization of ATPE has attracted interest due to the increase of performance, the possibility of operating in a continuous mode, and easy to scale-up by numbering-up approach. However, there is a gap in the literature with regard to the effects of flow patterns on the partitioning of biomolecules using microdevices. Thus, the present study investigated the ATPE of bovine serum albumin (BSA) and ampicillin, a beta-lactam antibiotic, under different flow patterns (slug, droplet and parallel) by means of microdevices. The microdevices were manufactured with circular tubes in a senoidal shape and the ATPE were prepared from the mixture between aqueous solutions of polyethylene glycol (PEG) and dibasic potassium phosphate. Using 1.80 mm tubes, slug, droplet and parallel flow patterns were observed only in the PEG 1500/dibasic potassium phosphate system, while the parallel flow pattern was not stabilized in systems involving PEG 4000 and PEG 6000. Experiments using a droplet flow pattern showed higher values of recovery (56% after 10 min) and partition coefficient of BSA compared to the slug and parallel flow patterns. In experiments involving ampicillin, the extraction performance was practically independent of the flow patterns. The BSA recovery behaved as an inverse function of the diameter tubes, which achieved slightly superior per- formance than the batch experiment (67% after 10 min) using droplet flow pattern, 0.51 mm tubes and residence time of 10 min (69%)engreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNinfo:eu-repo/semantics/openAccessCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.ufrn.br/bitstream/123456789/32345/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/32345/3/license.txte9597aa2854d128fd968be5edc8a28d9MD53123456789/323452024-03-19 01:02:05.01oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2024-03-19T04:02:05Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.pt_BR.fl_str_mv Effect of flow patterns on bovine serum albumin and ampicillin partitioning using aqueous two-phase systems in microdevice
title Effect of flow patterns on bovine serum albumin and ampicillin partitioning using aqueous two-phase systems in microdevice
spellingShingle Effect of flow patterns on bovine serum albumin and ampicillin partitioning using aqueous two-phase systems in microdevice
Santos, Everaldo Silvino dos
Microfluid
Extraction
Polyethylene glycol
Bovine serum albumin
Droplet flow pattern
title_short Effect of flow patterns on bovine serum albumin and ampicillin partitioning using aqueous two-phase systems in microdevice
title_full Effect of flow patterns on bovine serum albumin and ampicillin partitioning using aqueous two-phase systems in microdevice
title_fullStr Effect of flow patterns on bovine serum albumin and ampicillin partitioning using aqueous two-phase systems in microdevice
title_full_unstemmed Effect of flow patterns on bovine serum albumin and ampicillin partitioning using aqueous two-phase systems in microdevice
title_sort Effect of flow patterns on bovine serum albumin and ampicillin partitioning using aqueous two-phase systems in microdevice
author Santos, Everaldo Silvino dos
author_facet Santos, Everaldo Silvino dos
Pedro, Marcelo da Silva
Oliveira, LuizAndré Fialho
Padilha, Carlos Eduardo de Araújo
Oliveira, Jackson Araújo de
Souza, Domingos Fabiano de Santana
author_role author
author2 Pedro, Marcelo da Silva
Oliveira, LuizAndré Fialho
Padilha, Carlos Eduardo de Araújo
Oliveira, Jackson Araújo de
Souza, Domingos Fabiano de Santana
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, Everaldo Silvino dos
Pedro, Marcelo da Silva
Oliveira, LuizAndré Fialho
Padilha, Carlos Eduardo de Araújo
Oliveira, Jackson Araújo de
Souza, Domingos Fabiano de Santana
dc.subject.por.fl_str_mv Microfluid
Extraction
Polyethylene glycol
Bovine serum albumin
Droplet flow pattern
topic Microfluid
Extraction
Polyethylene glycol
Bovine serum albumin
Droplet flow pattern
description 2026
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-05-03T17:58:20Z
dc.date.issued.fl_str_mv 2021-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PEDRO, Marcelo da Silva; OLIVEIRA, Luiz André Fialho; PADILHA, Carlos Eduardo de Araújo; SANTOS, Everaldo Silvino dos; OLIVEIRA, Jackson Araújo de; SOUZA, Domingos Fabiano de Santana. Effect of flow patterns on bovine serum albumin and ampicillin partitioning using aqueous two-phase systems in microdevice. Separation and Purification Technology, [S.L.], v. 254, p. 117592, jan. 2021. Disponível em https://www.sciencedirect.com/science/article/abs/pii/S1383586620320669?via%3Dihub. Acesso em 30 mar. 2021. https://doi.org/10.1016/j.seppur.2020.117592
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/handle/123456789/32345
dc.identifier.issn.none.fl_str_mv 1383-5866
dc.identifier.doi.none.fl_str_mv 10.1016/j.seppur.2020.117592
identifier_str_mv PEDRO, Marcelo da Silva; OLIVEIRA, Luiz André Fialho; PADILHA, Carlos Eduardo de Araújo; SANTOS, Everaldo Silvino dos; OLIVEIRA, Jackson Araújo de; SOUZA, Domingos Fabiano de Santana. Effect of flow patterns on bovine serum albumin and ampicillin partitioning using aqueous two-phase systems in microdevice. Separation and Purification Technology, [S.L.], v. 254, p. 117592, jan. 2021. Disponível em https://www.sciencedirect.com/science/article/abs/pii/S1383586620320669?via%3Dihub. Acesso em 30 mar. 2021. https://doi.org/10.1016/j.seppur.2020.117592
1383-5866
10.1016/j.seppur.2020.117592
url https://repositorio.ufrn.br/handle/123456789/32345
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFRN
instname:Universidade Federal do Rio Grande do Norte (UFRN)
instacron:UFRN
instname_str Universidade Federal do Rio Grande do Norte (UFRN)
instacron_str UFRN
institution UFRN
reponame_str Repositório Institucional da UFRN
collection Repositório Institucional da UFRN
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