Design of a lipase-nano particle biocatalysts and its use in the kinetic resolution of medicament precursors

• Autor(a) principal: Bezerra, Rayanne Mendes
• Data de Publicação: 2017
• Outros Autores: Andrade Neto, Davino M., Galvão, Wesley S., Rios, Nathalia Saraiva, Carvalho, Ana Caroline Lustosa de Melo, Corrêa, Marcio Assolin, Bohn, Felipe, Fernandez-Lafuente, Roberto, Fechine, Pierre B.A., Mattos, Marcos Carlos de, Santos, José Cleiton Sousa dos, Gonçalves, Luciana R.B.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFRN
• Texto Completo: https://repositorio.ufrn.br/jspui/handle/123456789/29275
• topic: Biocatalyst; Superparamagnetic nanoparticles; 3-Amino-propyltriethoxysilane; Branched-polyethylenimine; Kinetic resolution

Superparamagnetic iron oxide nanoparticles (Fe3O4) were prepared by the co-precipitation method and functionalized with 3-amino-propyltriethoxysilane (APTES) or branched-polyethylenimine (PEI). After that, two parallels methods to immobilize the lipase from Thermomyces lanuginosus (TLL) were performed: the first one by ionic exchange and the second one by covalent attachment after the functionalization of the support with glutaraldehyde (GA). X-ray powder diffraction, magnetometry and infrared spectroscopy analysis were used to characterize the TLL preparations. These analyses showed that all samples presented superparamagnetic properties even after the immobilization procedure. The SPMN (superparamagnetic nanoparticle) @APTES covalent preparation had around 450 min of half-life time at pH 7.0 and 70 °C while that of the free enzyme was 46 min. These biocatalysts were evaluated in the kinetic resolution of rac-1-methyl-2-(2,6-dimethylphenoxy)ethyl acetate in different co-solvents (acetonitrile, isopropanol, ethyl ether and tetrahydrofuran). The best results were for the enzyme/substrate ratio of 2:1, in the presence of the ethyl ether or THF (20% v/v both) at 30 °C during 24 h with the SPMN@PEI-TLL biocatalyst. The conversion attained was 50% and the enantiomeric excess of the product was 99%. The new SPMN support are an excellent strategy to easy recovery of the biocatalyst by applying a magnetic field