Análise da resposta Th17 em líquen plano oral
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/jspui/handle/123456789/17122 |
Resumo: | Th17 cells have been strongly associated with the pathogenesis of several autoimmune and inflammatory diseases. IL-17 and IL-23 are important cytokines associated with this lineage. The aim of this study was to analyze, through immunohistochemical methods, the immunoexpression of IL-17 and IL-23 in the inflammatory infiltrate of oral lichen planus (OLP) lesion compared to that of inflammatory fibrous hyperplasia (IFH) and between clinical forms reticular and erosive of OLP. The sample included 41 cases of OLP, of which 23 were reticular and 18 erosive and 10 cases of IFH. The results were subjected to nonparametric statistical tests with a 5% significance level. In OLP lesions histomorphological analysis, the most common findings were: hyperparakeratinization, specimens with atrophic epithelium in erosive clinical form (p = 0.011), epithelial projections in most of reticular type of lesions, in addition Civatte bodies were identified in most samples of both clinical forms. For immunohistochemistry analysis, five fields with strong immunoreactivity for IL-17 and IL-23 were photomicrographed at 400x magnification, images were transferred to a computer where with ImageJ software®, lymphocytes that exhibited cytoplasmic immunostaining for these cytokines were counted. A mean was established after for each case. There was no statistically significant difference in the number of imunopositive lymphocytes for IL-17 and IL-23 among the group of OLP and IFH group, however a larger amount of lymphocytes imunopositive for IL-17 was found in the LPO group (p = 0.079) and significantly higher amounts of those lymphocytes were found in the erosive OLP when compared to the group of reticular OLP and IFH (p = 0.019). Furthermore, a marker epithelial immunopositivity for IL-17 was observed in OLP group. Although the results of this study do not permit the forceful assertion about the participation of Th17 lineage in OLP lesions, the findings of immunopositive lymphocytes counting for IL-17 and IL-23, which are potent proinflammatory cytokines, together with the the marked epithelial immunopositivity found for IL-17 in this study, suggest a possible role of this lineage in the pathogenesis of this disorder |
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Monteiro, Barbara Vanessa de Britohttp://lattes.cnpq.br/8314906045927707http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4707501Z3&dataRevisao=nullGodoy, Gustavo Pinahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4751939A2Silveira, Ericka Janine Dantas dahttp://lattes.cnpq.br/2186658404241838Miguel, Márcia Cristina da Costa2014-12-17T15:32:21Z2012-10-152014-12-17T15:32:21Z2012-02-24MONTEIRO, Barbara Vanessa de Brito. Análise da resposta Th17 em líquen plano oral. 2012. 108 f. Dissertação (Mestrado em Odontologia) - Universidade Federal do Rio Grande do Norte, Natal, 2012.https://repositorio.ufrn.br/jspui/handle/123456789/17122Th17 cells have been strongly associated with the pathogenesis of several autoimmune and inflammatory diseases. IL-17 and IL-23 are important cytokines associated with this lineage. The aim of this study was to analyze, through immunohistochemical methods, the immunoexpression of IL-17 and IL-23 in the inflammatory infiltrate of oral lichen planus (OLP) lesion compared to that of inflammatory fibrous hyperplasia (IFH) and between clinical forms reticular and erosive of OLP. The sample included 41 cases of OLP, of which 23 were reticular and 18 erosive and 10 cases of IFH. The results were subjected to nonparametric statistical tests with a 5% significance level. In OLP lesions histomorphological analysis, the most common findings were: hyperparakeratinization, specimens with atrophic epithelium in erosive clinical form (p = 0.011), epithelial projections in most of reticular type of lesions, in addition Civatte bodies were identified in most samples of both clinical forms. For immunohistochemistry analysis, five fields with strong immunoreactivity for IL-17 and IL-23 were photomicrographed at 400x magnification, images were transferred to a computer where with ImageJ software®, lymphocytes that exhibited cytoplasmic immunostaining for these cytokines were counted. A mean was established after for each case. There was no statistically significant difference in the number of imunopositive lymphocytes for IL-17 and IL-23 among the group of OLP and IFH group, however a larger amount of lymphocytes imunopositive for IL-17 was found in the LPO group (p = 0.079) and significantly higher amounts of those lymphocytes were found in the erosive OLP when compared to the group of reticular OLP and IFH (p = 0.019). Furthermore, a marker epithelial immunopositivity for IL-17 was observed in OLP group. Although the results of this study do not permit the forceful assertion about the participation of Th17 lineage in OLP lesions, the findings of immunopositive lymphocytes counting for IL-17 and IL-23, which are potent proinflammatory cytokines, together with the the marked epithelial immunopositivity found for IL-17 in this study, suggest a possible role of this lineage in the pathogenesis of this disorderAs células Th17 têm sido fortemente associadas com a patogenia de diversas doenças autoimunes e inflamatórias. A IL-17 e a IL-23 são importantes citocinas associadas com esta linhagem. O objetivo do presente trabalho foi analisar, através de métodos imunohistoquímicos, a imunoexpressão da IL-17 e da IL-23 no infiltrado inflamatório das lesões de líquen plano oral (LPO) comparando ao da hiperplasia fibrosa inflamatória (HFI) e entre as formas clínicas reticular e erosiva do LPO com o intuito de esclarecer se a linhagem Th17 participa da patogênese do LPO. Na amostra foram incluídos 41 casos de LPO, dos quais 23 eram reticulares e 18 erosivos, além de 10 casos de HFI. Os resultados foram submetidos a testes estatísticos não paramétricos com nível de significância de 5%. Na análise histomorfológica das lesões de LPO, observou-se predomínio de: lesões hiperparaceratinizadas, espécimes com epitélio atrófico na forma clínica erosiva (p=0,011), projeções epiteliais nas lesões do tipo reticular, além de corpos de Civatte identificados na maior parte da amostra de ambas as formas clínicas. Para o estudo imuno-histoquímico, cinco campos com forte imunorreatividade para a IL-17 e para a IL-23 foram fotomicrografados sob o aumento de 400x, as fotos foram transferidas para um computador onde com o auxílio do software ImageJ®, realizou-se a contagem dos linfócitos que exibiram imunomarcação citoplasmática para estas citocinas. Posteriormente, foi estabelecida uma média para cada caso. Não foram observadas diferenças estatisticamente significativas na quantidade de linfócitos imunopositivos para a IL-17 e para a IL-23 entre o grupo do LPO e da HFI, no entanto uma maior quantidade desses linfócitos para a IL-17 foi encontrada no grupo do LPO (p=0,079) e uma quantidade significativamente maior de linfócitos imunopositivos para a IL- 23 foi encontrada entre o grupo do LPO erosivo e da HFI (p=0,019). Além disto, foi observada uma marcante imunopositividade epitelial para a IL-17 no grupo do LPO. Ainda que os resultados do presente estudo não permitam a afirmação contundente da participação da linhagem Th17 nas lesões de LPO, os achados da contagem dos linfócitos imunopositivos para a IL-17 e para a IL-23, que são potentes citocinas pró-inflamatórias, somados à marcante imunopositividade epitelial encontrada para a IL-17 neste estudo, sugerem uma possível participação desta linhagem na patogênese desta desordemConselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal do Rio Grande do NortePrograma de Pós-Graduação em Patologia OralUFRNBROdontologiaLíquen plano bucalImunoistoquímicaCélulas Th17Interleucina-17Interleucina-23Lichen planusImmunohistochemistryTh17 cellsInterleukin-17Interleukin-23CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIAAnálise da resposta Th17 em líquen plano oralinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALBarbaraVBM_DISSERT.pdfapplication/pdf1563576https://repositorio.ufrn.br/bitstream/123456789/17122/1/BarbaraVBM_DISSERT.pdfc536e73cdfce0251b3446e74e0f9cc69MD51TEXTBarbaraVBM_DISSERT.pdf.txtBarbaraVBM_DISSERT.pdf.txtExtracted texttext/plain166914https://repositorio.ufrn.br/bitstream/123456789/17122/6/BarbaraVBM_DISSERT.pdf.txta1f4d876cc48f73e468398617f1a3d9cMD56THUMBNAILBarbaraVBM_DISSERT.pdf.jpgBarbaraVBM_DISSERT.pdf.jpgIM Thumbnailimage/jpeg2610https://repositorio.ufrn.br/bitstream/123456789/17122/7/BarbaraVBM_DISSERT.pdf.jpgaf4702da823f632157b108eb142a1127MD57123456789/171222017-11-04 13:12:21.461oai:https://repositorio.ufrn.br:123456789/17122Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2017-11-04T16:12:21Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.por.fl_str_mv |
Análise da resposta Th17 em líquen plano oral |
title |
Análise da resposta Th17 em líquen plano oral |
spellingShingle |
Análise da resposta Th17 em líquen plano oral Monteiro, Barbara Vanessa de Brito Líquen plano bucal Imunoistoquímica Células Th17 Interleucina-17 Interleucina-23 Lichen planus Immunohistochemistry Th17 cells Interleukin-17 Interleukin-23 CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
title_short |
Análise da resposta Th17 em líquen plano oral |
title_full |
Análise da resposta Th17 em líquen plano oral |
title_fullStr |
Análise da resposta Th17 em líquen plano oral |
title_full_unstemmed |
Análise da resposta Th17 em líquen plano oral |
title_sort |
Análise da resposta Th17 em líquen plano oral |
author |
Monteiro, Barbara Vanessa de Brito |
author_facet |
Monteiro, Barbara Vanessa de Brito |
author_role |
author |
dc.contributor.authorID.por.fl_str_mv |
|
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/8314906045927707 |
dc.contributor.advisorID.por.fl_str_mv |
|
dc.contributor.advisorLattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4707501Z3&dataRevisao=null |
dc.contributor.referees1.pt_BR.fl_str_mv |
Godoy, Gustavo Pina |
dc.contributor.referees1ID.por.fl_str_mv |
|
dc.contributor.referees1Lattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4751939A2 |
dc.contributor.referees2.pt_BR.fl_str_mv |
Silveira, Ericka Janine Dantas da |
dc.contributor.referees2ID.por.fl_str_mv |
|
dc.contributor.referees2Lattes.por.fl_str_mv |
http://lattes.cnpq.br/2186658404241838 |
dc.contributor.author.fl_str_mv |
Monteiro, Barbara Vanessa de Brito |
dc.contributor.advisor1.fl_str_mv |
Miguel, Márcia Cristina da Costa |
contributor_str_mv |
Miguel, Márcia Cristina da Costa |
dc.subject.por.fl_str_mv |
Líquen plano bucal Imunoistoquímica Células Th17 Interleucina-17 Interleucina-23 |
topic |
Líquen plano bucal Imunoistoquímica Células Th17 Interleucina-17 Interleucina-23 Lichen planus Immunohistochemistry Th17 cells Interleukin-17 Interleukin-23 CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
dc.subject.eng.fl_str_mv |
Lichen planus Immunohistochemistry Th17 cells Interleukin-17 Interleukin-23 |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
description |
Th17 cells have been strongly associated with the pathogenesis of several autoimmune and inflammatory diseases. IL-17 and IL-23 are important cytokines associated with this lineage. The aim of this study was to analyze, through immunohistochemical methods, the immunoexpression of IL-17 and IL-23 in the inflammatory infiltrate of oral lichen planus (OLP) lesion compared to that of inflammatory fibrous hyperplasia (IFH) and between clinical forms reticular and erosive of OLP. The sample included 41 cases of OLP, of which 23 were reticular and 18 erosive and 10 cases of IFH. The results were subjected to nonparametric statistical tests with a 5% significance level. In OLP lesions histomorphological analysis, the most common findings were: hyperparakeratinization, specimens with atrophic epithelium in erosive clinical form (p = 0.011), epithelial projections in most of reticular type of lesions, in addition Civatte bodies were identified in most samples of both clinical forms. For immunohistochemistry analysis, five fields with strong immunoreactivity for IL-17 and IL-23 were photomicrographed at 400x magnification, images were transferred to a computer where with ImageJ software®, lymphocytes that exhibited cytoplasmic immunostaining for these cytokines were counted. A mean was established after for each case. There was no statistically significant difference in the number of imunopositive lymphocytes for IL-17 and IL-23 among the group of OLP and IFH group, however a larger amount of lymphocytes imunopositive for IL-17 was found in the LPO group (p = 0.079) and significantly higher amounts of those lymphocytes were found in the erosive OLP when compared to the group of reticular OLP and IFH (p = 0.019). Furthermore, a marker epithelial immunopositivity for IL-17 was observed in OLP group. Although the results of this study do not permit the forceful assertion about the participation of Th17 lineage in OLP lesions, the findings of immunopositive lymphocytes counting for IL-17 and IL-23, which are potent proinflammatory cytokines, together with the the marked epithelial immunopositivity found for IL-17 in this study, suggest a possible role of this lineage in the pathogenesis of this disorder |
publishDate |
2012 |
dc.date.available.fl_str_mv |
2012-10-15 2014-12-17T15:32:21Z |
dc.date.issued.fl_str_mv |
2012-02-24 |
dc.date.accessioned.fl_str_mv |
2014-12-17T15:32:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MONTEIRO, Barbara Vanessa de Brito. Análise da resposta Th17 em líquen plano oral. 2012. 108 f. Dissertação (Mestrado em Odontologia) - Universidade Federal do Rio Grande do Norte, Natal, 2012. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/jspui/handle/123456789/17122 |
identifier_str_mv |
MONTEIRO, Barbara Vanessa de Brito. Análise da resposta Th17 em líquen plano oral. 2012. 108 f. Dissertação (Mestrado em Odontologia) - Universidade Federal do Rio Grande do Norte, Natal, 2012. |
url |
https://repositorio.ufrn.br/jspui/handle/123456789/17122 |
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por |
language |
por |
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info:eu-repo/semantics/openAccess |
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Universidade Federal do Rio Grande do Norte |
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Programa de Pós-Graduação em Patologia Oral |
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UFRN |
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BR |
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Odontologia |
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Universidade Federal do Rio Grande do Norte |
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