Effects of Purified Saccharomyces cerevisiae (1→3)-β-Glucan on Venous Ulcer Healing
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/jspui/handle/1/6249 |
Resumo: | Water-insoluble glucan was isolated from the baker’s yeast Saccharomyces cerevisiae. The yeast cells were treated with alkali and the residue then with acid. Chemical and NMR (1D and 2D) analyses showed that a linear (1→3)-β-glucan was purified that was not contaminated with other carbohydrates, proteins or phenolic compounds. The effects of the glucan on wound healing were assessed in human venous ulcers by histopathological analysis after 30 days of topical treatment. (1→3)-β-glucan enhanced ulcer healing and increased epithelial hyperplasia, as well as increased inflammatory cells, angiogenesis and fibroblast proliferation. In one patient who had an ulcer that would not heal for over 15 years, glucan treatment caused a 67.8% decrease in the area of the ulcer. This is the first study to investigate the effects of (1→3)-β-glucan on venous ulcer healing in humans; our findings suggest that this glucan is a potential natural biological response modifier in wound healing |
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Medeiros, Sarah Dantas VianaCordeiro, Sara LimaCavalcanti, Jéssica Escorel ChavesMelchuna, Karina MendesLima, Aleida Maria da SilvaAraújo Filho, IramiMedeiros, Aldo CunhaRocha, Keyla Borges FerreiraOliveira, Elizabeth MaiaFaria, Eduardo Dantas BaptistaSassaki, Guilherme LanziRocha, Hugo Alexandre OliveiraSales, Valéria Soraya Farias2013-08-02T15:57:37Z2013-08-02T15:57:37Z2012-07-02MEDEIROS, Aldo Cunha et al. Effects of Purified Saccharomyces cerevisiae (1→3)-β-Glucan on Venous Ulcer Healing. International Journal of Molecular Sciences, v. 13, p. 8142-8158, 2012. Disponível em:<http://www.mdpi.com/1422-0067/13/7/8142>. Acesso em: 01 ago. 2013.1422-0067https://repositorio.ufrn.br/jspui/handle/1/6249Water-insoluble glucan was isolated from the baker’s yeast Saccharomyces cerevisiae. The yeast cells were treated with alkali and the residue then with acid. Chemical and NMR (1D and 2D) analyses showed that a linear (1→3)-β-glucan was purified that was not contaminated with other carbohydrates, proteins or phenolic compounds. The effects of the glucan on wound healing were assessed in human venous ulcers by histopathological analysis after 30 days of topical treatment. (1→3)-β-glucan enhanced ulcer healing and increased epithelial hyperplasia, as well as increased inflammatory cells, angiogenesis and fibroblast proliferation. In one patient who had an ulcer that would not heal for over 15 years, glucan treatment caused a 67.8% decrease in the area of the ulcer. This is the first study to investigate the effects of (1→3)-β-glucan on venous ulcer healing in humans; our findings suggest that this glucan is a potential natural biological response modifier in wound healingengwater-insoluble glucanpolysaccharideyeastimmunomodulatortissue repairEffects of Purified Saccharomyces cerevisiae (1→3)-β-Glucan on Venous Ulcer Healinginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALEffectsPurifiedSaccharomyces_Medeiros_2012.pdfEffectsPurifiedSaccharomyces_Medeiros_2012.pdfapplication/pdf1334706https://repositorio.ufrn.br/bitstream/1/6249/1/EffectsPurifiedSaccharomyces_Medeiros_2012.pdfe6770f9e73e677e24ee9271e30a8a050MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.ufrn.br/bitstream/1/6249/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52TEXTAldoCM_Effects of purified_ijms-13-08142.pdf.txtAldoCM_Effects of purified_ijms-13-08142.pdf.txtExtracted texttext/plain37490https://repositorio.ufrn.br/bitstream/1/6249/7/AldoCM_Effects%20of%20purified_ijms-13-08142.pdf.txt99b7f8376cdc0bbe3cfa42fef769fbc4MD57THUMBNAILAldoCM_Effects of purified_ijms-13-08142.pdf.jpgAldoCM_Effects of purified_ijms-13-08142.pdf.jpgIM Thumbnailimage/jpeg8374https://repositorio.ufrn.br/bitstream/1/6249/8/AldoCM_Effects%20of%20purified_ijms-13-08142.pdf.jpg9569b541be8e3baefbd2ebf336a2aed6MD581/62492021-11-30 18:54:27.441oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2021-11-30T21:54:27Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
Effects of Purified Saccharomyces cerevisiae (1→3)-β-Glucan on Venous Ulcer Healing |
title |
Effects of Purified Saccharomyces cerevisiae (1→3)-β-Glucan on Venous Ulcer Healing |
spellingShingle |
Effects of Purified Saccharomyces cerevisiae (1→3)-β-Glucan on Venous Ulcer Healing Medeiros, Sarah Dantas Viana water-insoluble glucan polysaccharide yeast immunomodulator tissue repair |
title_short |
Effects of Purified Saccharomyces cerevisiae (1→3)-β-Glucan on Venous Ulcer Healing |
title_full |
Effects of Purified Saccharomyces cerevisiae (1→3)-β-Glucan on Venous Ulcer Healing |
title_fullStr |
Effects of Purified Saccharomyces cerevisiae (1→3)-β-Glucan on Venous Ulcer Healing |
title_full_unstemmed |
Effects of Purified Saccharomyces cerevisiae (1→3)-β-Glucan on Venous Ulcer Healing |
title_sort |
Effects of Purified Saccharomyces cerevisiae (1→3)-β-Glucan on Venous Ulcer Healing |
author |
Medeiros, Sarah Dantas Viana |
author_facet |
Medeiros, Sarah Dantas Viana Cordeiro, Sara Lima Cavalcanti, Jéssica Escorel Chaves Melchuna, Karina Mendes Lima, Aleida Maria da Silva Araújo Filho, Irami Medeiros, Aldo Cunha Rocha, Keyla Borges Ferreira Oliveira, Elizabeth Maia Faria, Eduardo Dantas Baptista Sassaki, Guilherme Lanzi Rocha, Hugo Alexandre Oliveira Sales, Valéria Soraya Farias |
author_role |
author |
author2 |
Cordeiro, Sara Lima Cavalcanti, Jéssica Escorel Chaves Melchuna, Karina Mendes Lima, Aleida Maria da Silva Araújo Filho, Irami Medeiros, Aldo Cunha Rocha, Keyla Borges Ferreira Oliveira, Elizabeth Maia Faria, Eduardo Dantas Baptista Sassaki, Guilherme Lanzi Rocha, Hugo Alexandre Oliveira Sales, Valéria Soraya Farias |
author2_role |
author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Medeiros, Sarah Dantas Viana Cordeiro, Sara Lima Cavalcanti, Jéssica Escorel Chaves Melchuna, Karina Mendes Lima, Aleida Maria da Silva Araújo Filho, Irami Medeiros, Aldo Cunha Rocha, Keyla Borges Ferreira Oliveira, Elizabeth Maia Faria, Eduardo Dantas Baptista Sassaki, Guilherme Lanzi Rocha, Hugo Alexandre Oliveira Sales, Valéria Soraya Farias |
dc.subject.por.fl_str_mv |
water-insoluble glucan polysaccharide yeast immunomodulator tissue repair |
topic |
water-insoluble glucan polysaccharide yeast immunomodulator tissue repair |
description |
Water-insoluble glucan was isolated from the baker’s yeast Saccharomyces cerevisiae. The yeast cells were treated with alkali and the residue then with acid. Chemical and NMR (1D and 2D) analyses showed that a linear (1→3)-β-glucan was purified that was not contaminated with other carbohydrates, proteins or phenolic compounds. The effects of the glucan on wound healing were assessed in human venous ulcers by histopathological analysis after 30 days of topical treatment. (1→3)-β-glucan enhanced ulcer healing and increased epithelial hyperplasia, as well as increased inflammatory cells, angiogenesis and fibroblast proliferation. In one patient who had an ulcer that would not heal for over 15 years, glucan treatment caused a 67.8% decrease in the area of the ulcer. This is the first study to investigate the effects of (1→3)-β-glucan on venous ulcer healing in humans; our findings suggest that this glucan is a potential natural biological response modifier in wound healing |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-07-02 |
dc.date.accessioned.fl_str_mv |
2013-08-02T15:57:37Z |
dc.date.available.fl_str_mv |
2013-08-02T15:57:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MEDEIROS, Aldo Cunha et al. Effects of Purified Saccharomyces cerevisiae (1→3)-β-Glucan on Venous Ulcer Healing. International Journal of Molecular Sciences, v. 13, p. 8142-8158, 2012. Disponível em:<http://www.mdpi.com/1422-0067/13/7/8142>. Acesso em: 01 ago. 2013. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/jspui/handle/1/6249 |
dc.identifier.issn.none.fl_str_mv |
1422-0067 |
identifier_str_mv |
MEDEIROS, Aldo Cunha et al. Effects of Purified Saccharomyces cerevisiae (1→3)-β-Glucan on Venous Ulcer Healing. International Journal of Molecular Sciences, v. 13, p. 8142-8158, 2012. Disponível em:<http://www.mdpi.com/1422-0067/13/7/8142>. Acesso em: 01 ago. 2013. 1422-0067 |
url |
https://repositorio.ufrn.br/jspui/handle/1/6249 |
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eng |
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