Avaliação da interleucina-6 e do metabolismo do ferro em 20 cães saudáveis

• Autor(a) principal: Medeiros, Nina da Cunha
• Data de Publicação: 2023
• Outros Autores: Locatelli Dittrich, Rosangela
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Medicina Veterinária (Recife. Online)
• Texto Completo: https://www.journals.ufrpe.br/index.php/medicinaveterinaria/article/view/5648
• topic: capacidade total de ligação do ferro; capacidade latente de ligação do ferro; transferrina; ferritina; inflamação

In inflammation, there is an increase in interleukin-6 (IL-6), a pro-inflammatory cytokine that stimulates the production of hepcidin. This hormone regulates iron metabolism, decreasing intestinal absorption and making iron unavailable inside cells. The main consequence of these alterations is anemia due to the unavailability of this mineral. To help the diagnosis, determine the prognosis and guide therapeutic decisions, laboratory evaluation of iron metabolism, hepcidin and IL-6 are important. The present study aimed to evaluate serum iron concentration, unsaturated iron-binding capacity (UIBC), total iron-binding capacity (TIBC), transferrin saturation (TS), IL-6 and hepcidin in healthy dogs. Twenty healthy adult dogs without specification of breed and sex and presenting C-reactive protein below 10 mg/L were evaluated. Iron concentration and UIBC were assessed by colorimetric method using Ferene and TIBC and IST were calculated. The determination of the serum concentration of IL-6 and hepcidin was performed by ELISA using specific kits for the canine species. The mean values obtained were: serum iron = 157.75 µg/dl; UIBC= 270.41 µg/dl; TIBC = 425.61 µg/dl; TS = 36% and IL-6 0.164 ng/ml. Of the 20 samples evaluated, only one showed a reading for hepcidin (16.9 ng/ml). There was no correlation between serum IL-6 concentrations and serum iron concentration, UIBC, TIBC and TS, suggesting that physiological variations of this cytokine do not interfere with iron metabolism in healthy dogs.