AUTOMATED AND MANUAL LEUKOCYTE DIFFERENTIAL COUNTS IN HEALTHY EQUINES

Detalhes bibliográficos
Autor(a) principal: Silva , Maria Fernanda Cazini
Data de Publicação: 2023
Outros Autores: Brito, Luma Maria Rovina, Martins, Beatriz de Azevedo, Lopes, Laryssa Eduarda de Campos, Duarte, Yasmin Cunha, Morais, Matheus Nogueira, Vasconcelos, Laura Arduino, Mendonça, Mariana Orlandini, Martins, Tainara de Oliveira, Montechiesi, Daniela Fernandez, Martins de Almeida, Breno Fernando
Tipo de documento: Artigo
Idioma: por
Título da fonte: Medicina Veterinária (Recife. Online)
Texto Completo: https://www.journals.ufrpe.br/index.php/medicinaveterinaria/article/view/5897
Resumo: The speed and convenience provided by automated cell counters are reasons for the increasing use of this technology in veterinary practice, with impedance methodology being one of the most commonly used in Brazil. In this regard, the objective of this study was to compare the automated differential leukocyte count obtained by impedance with the manual count obtained from the evaluation of blood smears in healthy horses. For this purpose, the automated differential leukocyte count using a veterinary cell counter (Abx Micros ESV 60, Horiba) was compared to the manual count using optical microscopy in 545 blood samples from healthy horses. Paired t-tests or Wilcoxon tests, Deming regression, Pearson or Spearman correlations, and Bland-Altman analysis were used, considering differences significant when p<0.05. The automated method showed higher concentrations of segmented neutrophils and monocytes, and lower concentrations of lymphocytes and eosinophils compared to the manual method. The mean error of the automated count was -10.26% for segmented neutrophils, 11.04% for lymphocytes, -41.39% for monocytes, and -10.84% for eosinophils, ranging from -200% to 161.4% depending on the cell type. There was a significant correlation between the methodologies only for the counts of segmented neutrophils, lymphocytes, and eosinophils. In conclusion, the manual differential leukocyte count in healthy horses cannot be replaced by the automated method, and the analysis of blood smears remains a fundamental tool for the proper interpretation of the leukogram in horses.
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spelling AUTOMATED AND MANUAL LEUKOCYTE DIFFERENTIAL COUNTS IN HEALTHY EQUINESAutomated impedance-based and manual leukocyte differential counts in healthy equineshorseleukogramimpedanceHorseLeukogramImpedanceThe speed and convenience provided by automated cell counters are reasons for the increasing use of this technology in veterinary practice, with impedance methodology being one of the most commonly used in Brazil. In this regard, the objective of this study was to compare the automated differential leukocyte count obtained by impedance with the manual count obtained from the evaluation of blood smears in healthy horses. For this purpose, the automated differential leukocyte count using a veterinary cell counter (Abx Micros ESV 60, Horiba) was compared to the manual count using optical microscopy in 545 blood samples from healthy horses. Paired t-tests or Wilcoxon tests, Deming regression, Pearson or Spearman correlations, and Bland-Altman analysis were used, considering differences significant when p<0.05. The automated method showed higher concentrations of segmented neutrophils and monocytes, and lower concentrations of lymphocytes and eosinophils compared to the manual method. The mean error of the automated count was -10.26% for segmented neutrophils, 11.04% for lymphocytes, -41.39% for monocytes, and -10.84% for eosinophils, ranging from -200% to 161.4% depending on the cell type. There was a significant correlation between the methodologies only for the counts of segmented neutrophils, lymphocytes, and eosinophils. In conclusion, the manual differential leukocyte count in healthy horses cannot be replaced by the automated method, and the analysis of blood smears remains a fundamental tool for the proper interpretation of the leukogram in horses.The speed and convenience provided by automated cell counters are reasons for the increasing use of this technology in veterinary practice, with impedance methodology being one of the most commonly used in Brazil. In this regard, the objective of this study was to compare the automated differential leukocyte count obtained by impedance with the manual count obtained from the evaluation of blood smears in healthy horses. For this purpose, the automated differential leukocyte count by impedance using a veterinary cell counter (Abx Micros ESV 60, Horiba) was compared to the manual count using optical microscopy in 545 blood samples from healthy horses. Paired t-tests or Wilcoxon tests, Deming regression, Pearson or Spearman correlations, and Bland-Altman analysis were used, considering differences significant when p<0.05. All optical microscopy counts were carried out by the same veterinary clinical pathologist with ten years of previous experience in the area. The automated method showed higher concentrations of segmented neutrophils and monocytes, and lower concentrations of lymphocytes and eosinophils compared to the manual method. The mean error of the automated count was -10.26% for segmented neutrophils, 11.04% for lymphocytes, -41.39% for monocytes, and -10.84% for eosinophils, ranging from -200% to 161.4% depending on the cell type. There was a significant correlation between the methodologies only for the counts of segmented neutrophils, lymphocytes, and eosinophils. In conclusion, the manual differential leukocyte count in healthy horses cannot be replaced by the automated method, and the analysis of blood smears remains a fundamental tool for the proper interpretation of the leukogram in horses.MEDICINA VETERINÁRIA2023-12-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.journals.ufrpe.br/index.php/medicinaveterinaria/article/view/589710.26605/medvet-v17n4-5897Medicina Veterinária; v. 17 n. 4 (2023): Medicina Veterinária; 249-2562675-66171809-467810.26605/medvet-v17n4reponame:Medicina Veterinária (Recife. Online)instname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPEporhttps://www.journals.ufrpe.br/index.php/medicinaveterinaria/article/view/5897/482485160Copyright (c) 2023 Maria Fernanda Cazini Silva , Luma Maria Rovina Brito, Beatriz de Azevedo Martins, Laryssa Eduarda de Campos Lopes, Yasmin Cunha Duarte, Matheus Nogueira Morais, Laura Arduino Vasconcelos, Mariana Orlandini Mendonça, Tainara de Oliveira Martins, Daniela Fernandez Montechiesi, Breno Fernando Martins de Almeidahttps://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessSilva , Maria Fernanda CaziniBrito, Luma Maria RovinaMartins, Beatriz de AzevedoLopes, Laryssa Eduarda de CamposDuarte, Yasmin CunhaMorais, Matheus NogueiraVasconcelos, Laura ArduinoMendonça, Mariana OrlandiniMartins, Tainara de OliveiraMontechiesi, Daniela FernandezMartins de Almeida, Breno Fernando2023-12-28T13:22:38Zoai:ojs.10.0.7.8:article/5897Revistahttps://www.journals.ufrpe.br/index.php/medicinaveterinaria/PUBhttps://www.journals.ufrpe.br/index.php/medicinaveterinaria/oairevmedvet@ufrpe.br1809-46782675-6617opendoar:2023-12-28T13:22:38Medicina Veterinária (Recife. Online) - Universidade Federal Rural de Pernambuco (UFRPE)false
dc.title.none.fl_str_mv AUTOMATED AND MANUAL LEUKOCYTE DIFFERENTIAL COUNTS IN HEALTHY EQUINES
Automated impedance-based and manual leukocyte differential counts in healthy equines
title AUTOMATED AND MANUAL LEUKOCYTE DIFFERENTIAL COUNTS IN HEALTHY EQUINES
spellingShingle AUTOMATED AND MANUAL LEUKOCYTE DIFFERENTIAL COUNTS IN HEALTHY EQUINES
Silva , Maria Fernanda Cazini
horse
leukogram
impedance
Horse
Leukogram
Impedance
title_short AUTOMATED AND MANUAL LEUKOCYTE DIFFERENTIAL COUNTS IN HEALTHY EQUINES
title_full AUTOMATED AND MANUAL LEUKOCYTE DIFFERENTIAL COUNTS IN HEALTHY EQUINES
title_fullStr AUTOMATED AND MANUAL LEUKOCYTE DIFFERENTIAL COUNTS IN HEALTHY EQUINES
title_full_unstemmed AUTOMATED AND MANUAL LEUKOCYTE DIFFERENTIAL COUNTS IN HEALTHY EQUINES
title_sort AUTOMATED AND MANUAL LEUKOCYTE DIFFERENTIAL COUNTS IN HEALTHY EQUINES
author Silva , Maria Fernanda Cazini
author_facet Silva , Maria Fernanda Cazini
Brito, Luma Maria Rovina
Martins, Beatriz de Azevedo
Lopes, Laryssa Eduarda de Campos
Duarte, Yasmin Cunha
Morais, Matheus Nogueira
Vasconcelos, Laura Arduino
Mendonça, Mariana Orlandini
Martins, Tainara de Oliveira
Montechiesi, Daniela Fernandez
Martins de Almeida, Breno Fernando
author_role author
author2 Brito, Luma Maria Rovina
Martins, Beatriz de Azevedo
Lopes, Laryssa Eduarda de Campos
Duarte, Yasmin Cunha
Morais, Matheus Nogueira
Vasconcelos, Laura Arduino
Mendonça, Mariana Orlandini
Martins, Tainara de Oliveira
Montechiesi, Daniela Fernandez
Martins de Almeida, Breno Fernando
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva , Maria Fernanda Cazini
Brito, Luma Maria Rovina
Martins, Beatriz de Azevedo
Lopes, Laryssa Eduarda de Campos
Duarte, Yasmin Cunha
Morais, Matheus Nogueira
Vasconcelos, Laura Arduino
Mendonça, Mariana Orlandini
Martins, Tainara de Oliveira
Montechiesi, Daniela Fernandez
Martins de Almeida, Breno Fernando
dc.subject.por.fl_str_mv horse
leukogram
impedance
Horse
Leukogram
Impedance
topic horse
leukogram
impedance
Horse
Leukogram
Impedance
description The speed and convenience provided by automated cell counters are reasons for the increasing use of this technology in veterinary practice, with impedance methodology being one of the most commonly used in Brazil. In this regard, the objective of this study was to compare the automated differential leukocyte count obtained by impedance with the manual count obtained from the evaluation of blood smears in healthy horses. For this purpose, the automated differential leukocyte count using a veterinary cell counter (Abx Micros ESV 60, Horiba) was compared to the manual count using optical microscopy in 545 blood samples from healthy horses. Paired t-tests or Wilcoxon tests, Deming regression, Pearson or Spearman correlations, and Bland-Altman analysis were used, considering differences significant when p<0.05. The automated method showed higher concentrations of segmented neutrophils and monocytes, and lower concentrations of lymphocytes and eosinophils compared to the manual method. The mean error of the automated count was -10.26% for segmented neutrophils, 11.04% for lymphocytes, -41.39% for monocytes, and -10.84% for eosinophils, ranging from -200% to 161.4% depending on the cell type. There was a significant correlation between the methodologies only for the counts of segmented neutrophils, lymphocytes, and eosinophils. In conclusion, the manual differential leukocyte count in healthy horses cannot be replaced by the automated method, and the analysis of blood smears remains a fundamental tool for the proper interpretation of the leukogram in horses.
publishDate 2023
dc.date.none.fl_str_mv 2023-12-28
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.journals.ufrpe.br/index.php/medicinaveterinaria/article/view/5897
10.26605/medvet-v17n4-5897
url https://www.journals.ufrpe.br/index.php/medicinaveterinaria/article/view/5897
identifier_str_mv 10.26605/medvet-v17n4-5897
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://www.journals.ufrpe.br/index.php/medicinaveterinaria/article/view/5897/482485160
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MEDICINA VETERINÁRIA
publisher.none.fl_str_mv MEDICINA VETERINÁRIA
dc.source.none.fl_str_mv Medicina Veterinária; v. 17 n. 4 (2023): Medicina Veterinária; 249-256
2675-6617
1809-4678
10.26605/medvet-v17n4
reponame:Medicina Veterinária (Recife. Online)
instname:Universidade Federal Rural de Pernambuco (UFRPE)
instacron:UFRPE
instname_str Universidade Federal Rural de Pernambuco (UFRPE)
instacron_str UFRPE
institution UFRPE
reponame_str Medicina Veterinária (Recife. Online)
collection Medicina Veterinária (Recife. Online)
repository.name.fl_str_mv Medicina Veterinária (Recife. Online) - Universidade Federal Rural de Pernambuco (UFRPE)
repository.mail.fl_str_mv revmedvet@ufrpe.br
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