Induction of somatic embryogenesis in two cultivars of anthurium analysed by scanning electron microscopy
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Agro@mbiente on-line |
Texto Completo: | https://revista.ufrr.br/agroambiente/article/view/5333 |
Resumo: | Somatic embryogenesis is an advantageous tool in the commercial production of micropropagated anthurium plantlets. As such, the aim of this study was to establish a protocol for the induction of somatic embryogenesis in Jureia and Luau cultivars. Defoliated nodal segments, 1.0 cm in length and containing one bud, were used as explants. The experimental design was completely randomised, in a 2 x 3 x 5 factorial scheme (cultivar: Jureia and Luau x auxin: 2,4-D, NAA and Picloram x concentration: 0, 2.5, 5.0, 7.5, and 10.0 μM), with 30 treatments in a scheme of plots split over time (15, 30, 45, 60, 75 and 90 days). The anatomy and percentage of embryogenic callus formation were analysed. The structures formed, analysed by scanning electron microscopy, corresponded to embryogenic calli. The Luau cultivar was superior in forming embryogenic calli. For the two cultivars, among the auxins under study, NAA demonstrated a greater induction potential for somatic embryogenesis, with the concentration of 7.5 μM giving the highest mean values. The 90-day evaluation period showed the maximum formation of embryogenic calli; however, mean values were fairly similar to the 75-day evaluation period. To induce embryogenic calli, therefore, it is suggested that the nodal segments be inoculated into a culture medium with added NAA growth regulator at a concentration of 7.5 μM, and that the explants remain in this medium for 75 days after inoculation. |
id |
UFRR-4_87072f12ab44725d0574085d432fa983 |
---|---|
oai_identifier_str |
oai:oai.revista.ufrr.br:article/5333 |
network_acronym_str |
UFRR-4 |
network_name_str |
Agro@mbiente on-line |
repository_id_str |
|
spelling |
Induction of somatic embryogenesis in two cultivars of anthurium analysed by scanning electron microscopyAnálise microestrutural. Calos embriogênicos. Micropropagação. Morfogênese in vitro.Somatic embryogenesis is an advantageous tool in the commercial production of micropropagated anthurium plantlets. As such, the aim of this study was to establish a protocol for the induction of somatic embryogenesis in Jureia and Luau cultivars. Defoliated nodal segments, 1.0 cm in length and containing one bud, were used as explants. The experimental design was completely randomised, in a 2 x 3 x 5 factorial scheme (cultivar: Jureia and Luau x auxin: 2,4-D, NAA and Picloram x concentration: 0, 2.5, 5.0, 7.5, and 10.0 μM), with 30 treatments in a scheme of plots split over time (15, 30, 45, 60, 75 and 90 days). The anatomy and percentage of embryogenic callus formation were analysed. The structures formed, analysed by scanning electron microscopy, corresponded to embryogenic calli. The Luau cultivar was superior in forming embryogenic calli. For the two cultivars, among the auxins under study, NAA demonstrated a greater induction potential for somatic embryogenesis, with the concentration of 7.5 μM giving the highest mean values. The 90-day evaluation period showed the maximum formation of embryogenic calli; however, mean values were fairly similar to the 75-day evaluation period. To induce embryogenic calli, therefore, it is suggested that the nodal segments be inoculated into a culture medium with added NAA growth regulator at a concentration of 7.5 μM, and that the explants remain in this medium for 75 days after inoculation.UFRR2019-05-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revista.ufrr.br/agroambiente/article/view/533310.18227/1982-8470ragro.v13i0.5333AGRO@MBIENTE ON-LINE JOURNALRAGR; Vol. 13 (2019): Edição Continuada; 1-13REVISTA AGRO@MBIENTE ON-LINE; Vol. 13 (2019): Edição Continuada; 1-13REVISTA AGRO@MBIENTE ON-LINE; v. 13 (2019): Edição Continuada; 1-131982-8470reponame:Agro@mbiente on-lineinstname:Universidade Federal de Roraima (UFRR)instacron:UFRRporhttps://revista.ufrr.br/agroambiente/article/view/5333/2613Copyright (c) 2019 REVISTA AGRO@MBIENTE ON-LINEinfo:eu-repo/semantics/openAccessdos Santos Melo, Priscila BezerraPortugal Pinto de Carvalho, Ana CristinaCampos de Magalhães Bertini, Cândida HermíniaMuniz, Celli RodriguesRossetti, Adroaldo Guimarães2019-12-06T15:37:15Zoai:oai.revista.ufrr.br:article/5333Revistahttps://revista.ufrr.br/index.php/agroambientePUBhttps://revista.ufrr.br/index.php/agroambiente/oai||scpuchoa@dsi.ufrr.br|| arcanjoalves@oi.com.br1982-84701982-8470opendoar:2019-12-06T15:37:15Agro@mbiente on-line - Universidade Federal de Roraima (UFRR)false |
dc.title.none.fl_str_mv |
Induction of somatic embryogenesis in two cultivars of anthurium analysed by scanning electron microscopy |
title |
Induction of somatic embryogenesis in two cultivars of anthurium analysed by scanning electron microscopy |
spellingShingle |
Induction of somatic embryogenesis in two cultivars of anthurium analysed by scanning electron microscopy dos Santos Melo, Priscila Bezerra Análise microestrutural. Calos embriogênicos. Micropropagação. Morfogênese in vitro. |
title_short |
Induction of somatic embryogenesis in two cultivars of anthurium analysed by scanning electron microscopy |
title_full |
Induction of somatic embryogenesis in two cultivars of anthurium analysed by scanning electron microscopy |
title_fullStr |
Induction of somatic embryogenesis in two cultivars of anthurium analysed by scanning electron microscopy |
title_full_unstemmed |
Induction of somatic embryogenesis in two cultivars of anthurium analysed by scanning electron microscopy |
title_sort |
Induction of somatic embryogenesis in two cultivars of anthurium analysed by scanning electron microscopy |
author |
dos Santos Melo, Priscila Bezerra |
author_facet |
dos Santos Melo, Priscila Bezerra Portugal Pinto de Carvalho, Ana Cristina Campos de Magalhães Bertini, Cândida Hermínia Muniz, Celli Rodrigues Rossetti, Adroaldo Guimarães |
author_role |
author |
author2 |
Portugal Pinto de Carvalho, Ana Cristina Campos de Magalhães Bertini, Cândida Hermínia Muniz, Celli Rodrigues Rossetti, Adroaldo Guimarães |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
dos Santos Melo, Priscila Bezerra Portugal Pinto de Carvalho, Ana Cristina Campos de Magalhães Bertini, Cândida Hermínia Muniz, Celli Rodrigues Rossetti, Adroaldo Guimarães |
dc.subject.por.fl_str_mv |
Análise microestrutural. Calos embriogênicos. Micropropagação. Morfogênese in vitro. |
topic |
Análise microestrutural. Calos embriogênicos. Micropropagação. Morfogênese in vitro. |
description |
Somatic embryogenesis is an advantageous tool in the commercial production of micropropagated anthurium plantlets. As such, the aim of this study was to establish a protocol for the induction of somatic embryogenesis in Jureia and Luau cultivars. Defoliated nodal segments, 1.0 cm in length and containing one bud, were used as explants. The experimental design was completely randomised, in a 2 x 3 x 5 factorial scheme (cultivar: Jureia and Luau x auxin: 2,4-D, NAA and Picloram x concentration: 0, 2.5, 5.0, 7.5, and 10.0 μM), with 30 treatments in a scheme of plots split over time (15, 30, 45, 60, 75 and 90 days). The anatomy and percentage of embryogenic callus formation were analysed. The structures formed, analysed by scanning electron microscopy, corresponded to embryogenic calli. The Luau cultivar was superior in forming embryogenic calli. For the two cultivars, among the auxins under study, NAA demonstrated a greater induction potential for somatic embryogenesis, with the concentration of 7.5 μM giving the highest mean values. The 90-day evaluation period showed the maximum formation of embryogenic calli; however, mean values were fairly similar to the 75-day evaluation period. To induce embryogenic calli, therefore, it is suggested that the nodal segments be inoculated into a culture medium with added NAA growth regulator at a concentration of 7.5 μM, and that the explants remain in this medium for 75 days after inoculation. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-05-03 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revista.ufrr.br/agroambiente/article/view/5333 10.18227/1982-8470ragro.v13i0.5333 |
url |
https://revista.ufrr.br/agroambiente/article/view/5333 |
identifier_str_mv |
10.18227/1982-8470ragro.v13i0.5333 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://revista.ufrr.br/agroambiente/article/view/5333/2613 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2019 REVISTA AGRO@MBIENTE ON-LINE info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2019 REVISTA AGRO@MBIENTE ON-LINE |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UFRR |
publisher.none.fl_str_mv |
UFRR |
dc.source.none.fl_str_mv |
AGRO@MBIENTE ON-LINE JOURNALRAGR; Vol. 13 (2019): Edição Continuada; 1-13 REVISTA AGRO@MBIENTE ON-LINE; Vol. 13 (2019): Edição Continuada; 1-13 REVISTA AGRO@MBIENTE ON-LINE; v. 13 (2019): Edição Continuada; 1-13 1982-8470 reponame:Agro@mbiente on-line instname:Universidade Federal de Roraima (UFRR) instacron:UFRR |
instname_str |
Universidade Federal de Roraima (UFRR) |
instacron_str |
UFRR |
institution |
UFRR |
reponame_str |
Agro@mbiente on-line |
collection |
Agro@mbiente on-line |
repository.name.fl_str_mv |
Agro@mbiente on-line - Universidade Federal de Roraima (UFRR) |
repository.mail.fl_str_mv |
||scpuchoa@dsi.ufrr.br|| arcanjoalves@oi.com.br |
_version_ |
1799770041481166848 |