Types and concentrations of cytokinins in the micropropagation of Anthurium maricense
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Agro@mbiente on-line |
Texto Completo: | https://revista.ufrr.br/agroambiente/article/view/4671 |
Resumo: | Faced with the demand for plants potted for their foliage, Anthurium maricense is seen as a viable option. However, most of the studies on obtaining micropropagated plantlets are for A. andraeanum, with nothing yet reported for A. maricense. The aim of this study therefore, was to evaluate the effect of four cytokinins in different concentrations, on the in vitro induction of shoots from nodal segments of A. maricense. The experimental design was completely randomised in a 4 x 4 factorial scheme, with four cytokinins (BAP, ZEA, CIN and 2iP) and 4 concentrations (0, 2.22, 4.44 and 6.66 μM), for a total of 16 treatments, with 6 replications of five test tubes, and using one nodal segment. Cultures were kept in a growth room at 25 ± 2°C, a photoperiod of 16 h and a light intensity of 30 μmolm-2 s-1 for 60 days. After this period, the number of shoots formed per node was evaluated. The addition of a cytokinin to the culture medium was determinant for the in vitro regeneration of shoots in A. maricense. The greatest estimated number of shoot formations in A. maricense were obtained in the culture media containing ZEA (3.87) and BAP (3.30), both at concentration of 6.66 μM. |
id |
UFRR-4_920ba0222f03c7cb8d1176cfba64c4e5 |
---|---|
oai_identifier_str |
oai:oai.revista.ufrr.br:article/4671 |
network_acronym_str |
UFRR-4 |
network_name_str |
Agro@mbiente on-line |
repository_id_str |
|
spelling |
Types and concentrations of cytokinins in the micropropagation of Anthurium maricenseAnthurium. Araceae. Tissue culture. Floriculture. In vitro regeneration.Faced with the demand for plants potted for their foliage, Anthurium maricense is seen as a viable option. However, most of the studies on obtaining micropropagated plantlets are for A. andraeanum, with nothing yet reported for A. maricense. The aim of this study therefore, was to evaluate the effect of four cytokinins in different concentrations, on the in vitro induction of shoots from nodal segments of A. maricense. The experimental design was completely randomised in a 4 x 4 factorial scheme, with four cytokinins (BAP, ZEA, CIN and 2iP) and 4 concentrations (0, 2.22, 4.44 and 6.66 μM), for a total of 16 treatments, with 6 replications of five test tubes, and using one nodal segment. Cultures were kept in a growth room at 25 ± 2°C, a photoperiod of 16 h and a light intensity of 30 μmolm-2 s-1 for 60 days. After this period, the number of shoots formed per node was evaluated. The addition of a cytokinin to the culture medium was determinant for the in vitro regeneration of shoots in A. maricense. The greatest estimated number of shoot formations in A. maricense were obtained in the culture media containing ZEA (3.87) and BAP (3.30), both at concentration of 6.66 μM.UFRR2018-06-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revista.ufrr.br/agroambiente/article/view/467110.18227/1982-8470ragro.v12i2.4671AGRO@MBIENTE ON-LINE JOURNALRAGR; Vol. 12 No. 2 (2018): ABRIL-JUNHO; 117-123REVISTA AGRO@MBIENTE ON-LINE; Vol. 12 Núm. 2 (2018): ABRIL-JUNHO; 117-123REVISTA AGRO@MBIENTE ON-LINE; v. 12 n. 2 (2018): ABRIL-JUNHO; 117-1231982-8470reponame:Agro@mbiente on-lineinstname:Universidade Federal de Roraima (UFRR)instacron:UFRRporhttps://revista.ufrr.br/agroambiente/article/view/4671/2499Copyright (c) 2018 REVISTA AGRO@MBIENTE ON-LINEinfo:eu-repo/semantics/openAccessSerafim, Cecília MoreiraCampos, Arlene Santistebandos Santos Melo, Priscila BezerraRibeiro de Castro, Ana CecíliaPortugal Pinto de Carvalho, Ana Cristina2018-06-30T18:54:13Zoai:oai.revista.ufrr.br:article/4671Revistahttps://revista.ufrr.br/index.php/agroambientePUBhttps://revista.ufrr.br/index.php/agroambiente/oai||scpuchoa@dsi.ufrr.br|| arcanjoalves@oi.com.br1982-84701982-8470opendoar:2018-06-30T18:54:13Agro@mbiente on-line - Universidade Federal de Roraima (UFRR)false |
dc.title.none.fl_str_mv |
Types and concentrations of cytokinins in the micropropagation of Anthurium maricense |
title |
Types and concentrations of cytokinins in the micropropagation of Anthurium maricense |
spellingShingle |
Types and concentrations of cytokinins in the micropropagation of Anthurium maricense Serafim, Cecília Moreira Anthurium. Araceae. Tissue culture. Floriculture. In vitro regeneration. |
title_short |
Types and concentrations of cytokinins in the micropropagation of Anthurium maricense |
title_full |
Types and concentrations of cytokinins in the micropropagation of Anthurium maricense |
title_fullStr |
Types and concentrations of cytokinins in the micropropagation of Anthurium maricense |
title_full_unstemmed |
Types and concentrations of cytokinins in the micropropagation of Anthurium maricense |
title_sort |
Types and concentrations of cytokinins in the micropropagation of Anthurium maricense |
author |
Serafim, Cecília Moreira |
author_facet |
Serafim, Cecília Moreira Campos, Arlene Santisteban dos Santos Melo, Priscila Bezerra Ribeiro de Castro, Ana Cecília Portugal Pinto de Carvalho, Ana Cristina |
author_role |
author |
author2 |
Campos, Arlene Santisteban dos Santos Melo, Priscila Bezerra Ribeiro de Castro, Ana Cecília Portugal Pinto de Carvalho, Ana Cristina |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Serafim, Cecília Moreira Campos, Arlene Santisteban dos Santos Melo, Priscila Bezerra Ribeiro de Castro, Ana Cecília Portugal Pinto de Carvalho, Ana Cristina |
dc.subject.por.fl_str_mv |
Anthurium. Araceae. Tissue culture. Floriculture. In vitro regeneration. |
topic |
Anthurium. Araceae. Tissue culture. Floriculture. In vitro regeneration. |
description |
Faced with the demand for plants potted for their foliage, Anthurium maricense is seen as a viable option. However, most of the studies on obtaining micropropagated plantlets are for A. andraeanum, with nothing yet reported for A. maricense. The aim of this study therefore, was to evaluate the effect of four cytokinins in different concentrations, on the in vitro induction of shoots from nodal segments of A. maricense. The experimental design was completely randomised in a 4 x 4 factorial scheme, with four cytokinins (BAP, ZEA, CIN and 2iP) and 4 concentrations (0, 2.22, 4.44 and 6.66 μM), for a total of 16 treatments, with 6 replications of five test tubes, and using one nodal segment. Cultures were kept in a growth room at 25 ± 2°C, a photoperiod of 16 h and a light intensity of 30 μmolm-2 s-1 for 60 days. After this period, the number of shoots formed per node was evaluated. The addition of a cytokinin to the culture medium was determinant for the in vitro regeneration of shoots in A. maricense. The greatest estimated number of shoot formations in A. maricense were obtained in the culture media containing ZEA (3.87) and BAP (3.30), both at concentration of 6.66 μM. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-06-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revista.ufrr.br/agroambiente/article/view/4671 10.18227/1982-8470ragro.v12i2.4671 |
url |
https://revista.ufrr.br/agroambiente/article/view/4671 |
identifier_str_mv |
10.18227/1982-8470ragro.v12i2.4671 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://revista.ufrr.br/agroambiente/article/view/4671/2499 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 REVISTA AGRO@MBIENTE ON-LINE info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 REVISTA AGRO@MBIENTE ON-LINE |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UFRR |
publisher.none.fl_str_mv |
UFRR |
dc.source.none.fl_str_mv |
AGRO@MBIENTE ON-LINE JOURNALRAGR; Vol. 12 No. 2 (2018): ABRIL-JUNHO; 117-123 REVISTA AGRO@MBIENTE ON-LINE; Vol. 12 Núm. 2 (2018): ABRIL-JUNHO; 117-123 REVISTA AGRO@MBIENTE ON-LINE; v. 12 n. 2 (2018): ABRIL-JUNHO; 117-123 1982-8470 reponame:Agro@mbiente on-line instname:Universidade Federal de Roraima (UFRR) instacron:UFRR |
instname_str |
Universidade Federal de Roraima (UFRR) |
instacron_str |
UFRR |
institution |
UFRR |
reponame_str |
Agro@mbiente on-line |
collection |
Agro@mbiente on-line |
repository.name.fl_str_mv |
Agro@mbiente on-line - Universidade Federal de Roraima (UFRR) |
repository.mail.fl_str_mv |
||scpuchoa@dsi.ufrr.br|| arcanjoalves@oi.com.br |
_version_ |
1789436778147479552 |