Metab?litos especiais isolados de Eugenia copacabanensis Kiaersk (Myrtaceae) e avalia??o de atividades antioxidante e antimicrobiana

Detalhes bibliográficos
Autor(a) principal: Carvalho Junior, Almir Ribeiro de
Data de Publicação: 2013
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
Texto Completo: https://tede.ufrrj.br/jspui/handle/jspui/3459
Resumo: The genus Eugenia (Myrtaceae) comprises about 550 species of trees and shrubs, mainly found in Central and South America. Several species are used in folk medicine to treat diseases such as diabetes, chronic diarrhea, body aches, skin rash, etc. This has motivated diverse studies related to both the chemical composition and the biological properties of the genus. The objective of this work was to study the chemical profile of the species Eugenia copacabanensis, besides evaluating its antioxidant and atimicrobiano potential. The compounds described in this work were isolated from chromatographic techniques and identified by 1H and 13C NMR, GC-MS experiments besides comparison with literature data. From leaves of E. copacabanenis were isolated and -amyrin, ursolic acid, 30-hydroxy-ursolic acid (rubif?lico), betulin, -sitosterol, -sitosterol glycoside, clovanodiol, globulol, viridiflorol and the flavonoids kaempferol-3-O-ramnosideo, quercetin-3-O-arabinoside, and quercetin. While from branches were isolated, friedelin, friedelan-3,4-lactone, taraxerol, octacosanyl coumarate, heptacosanyl coumarate, hexacosanyl coumarate and teracosanyl coumarate. From acetylation and methylation reactions of acid 30-hydroxy-ursolic it were obtained: diacetyl rubifolic acid methyl diacetyl rubifolato respectively. The antioxidant activity of the extracts and fractions was evaluated by DPPH and -carotene-linoleic acid methods. In both cases, we obtained excellent results with EC50 values ranging between 0.92 ?0.04 and 40.7 ? 1.0 g/ml and % inhibition between 28.7 ?7.9 to 90.0 ? 0.1%. Antimicrobial activity was evaluated against the microorganisms, Staphylococcus aureus, Candida albicans, Cryptococcus neoformans and Rhizopus oryzae and extracts and fractions of E. copacabanensis were not active until the concentration of 2.5 mg / mL.
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spelling Carvalho, Mario Geraldo de257.152.327-91http://lattes.cnpq.br/9794451665032168Figueiredo, Maria RaquelOliveira, Rodrigo Rodrigues deOliveira, Marcia Cristina Campos de125.085.417-29http://lattes.cnpq.br/7191491222073956Carvalho Junior, Almir Ribeiro de2020-04-27T14:46:09Z2013-04-16CARVALHO JUNIOR, Almir Ribeiro de. Metab?litos Especiais isolados de Eugenia copacabanensis Kiaersk (Myrtaceae) e avalia??o de atividades antioxidante e antimicrobiana. 2013. 126 f. Disserta??o (Mestrado em Qu?mica) - Instituto de Ci?ncias Exatas, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2013.https://tede.ufrrj.br/jspui/handle/jspui/3459The genus Eugenia (Myrtaceae) comprises about 550 species of trees and shrubs, mainly found in Central and South America. Several species are used in folk medicine to treat diseases such as diabetes, chronic diarrhea, body aches, skin rash, etc. This has motivated diverse studies related to both the chemical composition and the biological properties of the genus. The objective of this work was to study the chemical profile of the species Eugenia copacabanensis, besides evaluating its antioxidant and atimicrobiano potential. The compounds described in this work were isolated from chromatographic techniques and identified by 1H and 13C NMR, GC-MS experiments besides comparison with literature data. From leaves of E. copacabanenis were isolated and -amyrin, ursolic acid, 30-hydroxy-ursolic acid (rubif?lico), betulin, -sitosterol, -sitosterol glycoside, clovanodiol, globulol, viridiflorol and the flavonoids kaempferol-3-O-ramnosideo, quercetin-3-O-arabinoside, and quercetin. While from branches were isolated, friedelin, friedelan-3,4-lactone, taraxerol, octacosanyl coumarate, heptacosanyl coumarate, hexacosanyl coumarate and teracosanyl coumarate. From acetylation and methylation reactions of acid 30-hydroxy-ursolic it were obtained: diacetyl rubifolic acid methyl diacetyl rubifolato respectively. The antioxidant activity of the extracts and fractions was evaluated by DPPH and -carotene-linoleic acid methods. In both cases, we obtained excellent results with EC50 values ranging between 0.92 ?0.04 and 40.7 ? 1.0 g/ml and % inhibition between 28.7 ?7.9 to 90.0 ? 0.1%. Antimicrobial activity was evaluated against the microorganisms, Staphylococcus aureus, Candida albicans, Cryptococcus neoformans and Rhizopus oryzae and extracts and fractions of E. copacabanensis were not active until the concentration of 2.5 mg / mL.O g?nero Eugenia (Myrtaceae) compreende cerca 550 esp?cies de ?rvores e arbustos, principalmente, encontrados nas Am?ricas do Sul e Central. V?rias esp?cies do g?nero s?o usadas na medicina popular no tratamento de enfermidades, tais como: diabetes, diarreia cr?nica, dores no corpo, irrita??o na pele, etc. Isto tem motivado diversos estudos relacionados tanto a composi??o qu?mica, quanto ?s propriedades biol?gicas do g?nero. O objetivo deste trabalho foi estudar o perfil qu?mico da esp?cie Eugenia copacabanensis Kiaersk, al?m de avaliar o potencial antioxidante e atimicrobiano da mesma. As subst?ncias descritas nesse trabalho foram isoladas a partir de t?cnicas cromatogr?ficas e identificadas atrav?s de experimentos de RMN de 1H e 13C e compara??o com dados da literatura. Das folhas de E. copacabanenis foram isolados os triterpenos e -amirina, ?cido urs?lico, ?cido 30-hidroxi-urs?lico (?cido rubif?lico) e betulina, os sesquiterpenos clovanodiol, globulol, viridiflorol, esteroides -sitosterol, -sitosterol glicosilado e flavonoides canferol-3-O-ramnosideo, quercetina-3-O-arabinosideo, quercetina. Enquanto que dos galhos foram isolados, friedelina, friedelan-3,4-lactona, taraxerol e os cumaratos de octacosanila, heptacosanila, hexacosanila e teracosanila. A partir de rea??es de acetila??o e metila??o do ?cido 30-hidroxi-urs?lico foram obtidos dos derivados: o ?cido diacetil rubif?lico e diacetil metil rubifolato, respectivamente. A atividade antioxidante dos extratos e fra??es foi avaliada pelos m?todos DPPH e -caroteno-?cido linoleico. Em ambos os casos, obteve-se ?timos resultados, com valores de CE50 variando entre 0,92?0,04 e 40,7?1,0 g/mL e % de inibi??o de degrada??o entre 28,7?7,9 a 90,0?0,1%. A atividade antimicrobiana foi avaliada frente aos microorganismos , Candida albicans, Cryptococcus neoformans e Rhizopus oryzae e os extratos e fra??es de E. copacabanensis n?o foram ativos at? a concentra??o de 2,5 mg/mL.Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2020-04-27T14:46:08Z No. of bitstreams: 1 2013 - Almir Ribeiro de Carvalho Junior.pdf: 4989381 bytes, checksum: a1fb407e438d1562ab935c38fb3637f6 (MD5)Made available in DSpace on 2020-04-27T14:46:09Z (GMT). No. of bitstreams: 1 2013 - Almir Ribeiro de Carvalho Junior.pdf: 4989381 bytes, checksum: a1fb407e438d1562ab935c38fb3637f6 (MD5) Previous issue date: 2013-04-16Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico, CNPq, Brasilapplication/pdfhttps://tede.ufrrj.br/retrieve/14376/2013%20-%20Almir%20Ribeiro%20de%20Carvalho%20Junior.pdf.jpghttps://tede.ufrrj.br/retrieve/17428/2013%20-%20Almir%20Ribeiro%20de%20Carvalho%20Junior.pdf.jpghttps://tede.ufrrj.br/retrieve/23770/2013%20-%20Almir%20Ribeiro%20de%20Carvalho%20Junior.pdf.jpghttps://tede.ufrrj.br/retrieve/30138/2013%20-%20Almir%20Ribeiro%20de%20Carvalho%20Junior.pdf.jpghttps://tede.ufrrj.br/retrieve/36576/2013%20-%20Almir%20Ribeiro%20de%20Carvalho%20Junior.pdf.jpghttps://tede.ufrrj.br/retrieve/42960/2013%20-%20Almir%20Ribeiro%20de%20Carvalho%20Junior.pdf.jpghttps://tede.ufrrj.br/retrieve/49260/2013%20-%20Almir%20Ribeiro%20de%20Carvalho%20Junior.pdf.jpghttps://tede.ufrrj.br/retrieve/55700/2013%20-%20Almir%20Ribeiro%20de%20Carvalho%20Junior.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Qu?micaUFRRJBrasilInstituto de Ci?ncias ExatasAHN, M. 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dc.title.por.fl_str_mv Metab?litos especiais isolados de Eugenia copacabanensis Kiaersk (Myrtaceae) e avalia??o de atividades antioxidante e antimicrobiana
dc.title.alternative.eng.fl_str_mv Special metabolites isolated from Eugenia copacabanensis Kiaersk (Myrtaceae) and evaluation of antioxidant and antimicrobial activities
title Metab?litos especiais isolados de Eugenia copacabanensis Kiaersk (Myrtaceae) e avalia??o de atividades antioxidante e antimicrobiana
spellingShingle Metab?litos especiais isolados de Eugenia copacabanensis Kiaersk (Myrtaceae) e avalia??o de atividades antioxidante e antimicrobiana
Carvalho Junior, Almir Ribeiro de
E. copacabanensis
triterpenos
atividade antioxidante
E. copacabanensis
triterpenes
antioxidant activity
Qu?mica
title_short Metab?litos especiais isolados de Eugenia copacabanensis Kiaersk (Myrtaceae) e avalia??o de atividades antioxidante e antimicrobiana
title_full Metab?litos especiais isolados de Eugenia copacabanensis Kiaersk (Myrtaceae) e avalia??o de atividades antioxidante e antimicrobiana
title_fullStr Metab?litos especiais isolados de Eugenia copacabanensis Kiaersk (Myrtaceae) e avalia??o de atividades antioxidante e antimicrobiana
title_full_unstemmed Metab?litos especiais isolados de Eugenia copacabanensis Kiaersk (Myrtaceae) e avalia??o de atividades antioxidante e antimicrobiana
title_sort Metab?litos especiais isolados de Eugenia copacabanensis Kiaersk (Myrtaceae) e avalia??o de atividades antioxidante e antimicrobiana
author Carvalho Junior, Almir Ribeiro de
author_facet Carvalho Junior, Almir Ribeiro de
author_role author
dc.contributor.advisor1.fl_str_mv Carvalho, Mario Geraldo de
dc.contributor.advisor1ID.fl_str_mv 257.152.327-91
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/9794451665032168
dc.contributor.referee1.fl_str_mv Figueiredo, Maria Raquel
dc.contributor.referee2.fl_str_mv Oliveira, Rodrigo Rodrigues de
dc.contributor.referee3.fl_str_mv Oliveira, Marcia Cristina Campos de
dc.contributor.authorID.fl_str_mv 125.085.417-29
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/7191491222073956
dc.contributor.author.fl_str_mv Carvalho Junior, Almir Ribeiro de
contributor_str_mv Carvalho, Mario Geraldo de
Figueiredo, Maria Raquel
Oliveira, Rodrigo Rodrigues de
Oliveira, Marcia Cristina Campos de
dc.subject.por.fl_str_mv E. copacabanensis
triterpenos
atividade antioxidante
topic E. copacabanensis
triterpenos
atividade antioxidante
E. copacabanensis
triterpenes
antioxidant activity
Qu?mica
dc.subject.eng.fl_str_mv E. copacabanensis
triterpenes
antioxidant activity
dc.subject.cnpq.fl_str_mv Qu?mica
description The genus Eugenia (Myrtaceae) comprises about 550 species of trees and shrubs, mainly found in Central and South America. Several species are used in folk medicine to treat diseases such as diabetes, chronic diarrhea, body aches, skin rash, etc. This has motivated diverse studies related to both the chemical composition and the biological properties of the genus. The objective of this work was to study the chemical profile of the species Eugenia copacabanensis, besides evaluating its antioxidant and atimicrobiano potential. The compounds described in this work were isolated from chromatographic techniques and identified by 1H and 13C NMR, GC-MS experiments besides comparison with literature data. From leaves of E. copacabanenis were isolated and -amyrin, ursolic acid, 30-hydroxy-ursolic acid (rubif?lico), betulin, -sitosterol, -sitosterol glycoside, clovanodiol, globulol, viridiflorol and the flavonoids kaempferol-3-O-ramnosideo, quercetin-3-O-arabinoside, and quercetin. While from branches were isolated, friedelin, friedelan-3,4-lactone, taraxerol, octacosanyl coumarate, heptacosanyl coumarate, hexacosanyl coumarate and teracosanyl coumarate. From acetylation and methylation reactions of acid 30-hydroxy-ursolic it were obtained: diacetyl rubifolic acid methyl diacetyl rubifolato respectively. The antioxidant activity of the extracts and fractions was evaluated by DPPH and -carotene-linoleic acid methods. In both cases, we obtained excellent results with EC50 values ranging between 0.92 ?0.04 and 40.7 ? 1.0 g/ml and % inhibition between 28.7 ?7.9 to 90.0 ? 0.1%. Antimicrobial activity was evaluated against the microorganisms, Staphylococcus aureus, Candida albicans, Cryptococcus neoformans and Rhizopus oryzae and extracts and fractions of E. copacabanensis were not active until the concentration of 2.5 mg / mL.
publishDate 2013
dc.date.issued.fl_str_mv 2013-04-16
dc.date.accessioned.fl_str_mv 2020-04-27T14:46:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv CARVALHO JUNIOR, Almir Ribeiro de. Metab?litos Especiais isolados de Eugenia copacabanensis Kiaersk (Myrtaceae) e avalia??o de atividades antioxidante e antimicrobiana. 2013. 126 f. Disserta??o (Mestrado em Qu?mica) - Instituto de Ci?ncias Exatas, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2013.
dc.identifier.uri.fl_str_mv https://tede.ufrrj.br/jspui/handle/jspui/3459
identifier_str_mv CARVALHO JUNIOR, Almir Ribeiro de. Metab?litos Especiais isolados de Eugenia copacabanensis Kiaersk (Myrtaceae) e avalia??o de atividades antioxidante e antimicrobiana. 2013. 126 f. Disserta??o (Mestrado em Qu?mica) - Instituto de Ci?ncias Exatas, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2013.
url https://tede.ufrrj.br/jspui/handle/jspui/3459
dc.language.iso.fl_str_mv por
language por
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