Metabolismo de nitrog?nio em plantas de milho inoculadas com dois g?neros de bact?rias diazotr?ficas

Detalhes bibliográficos
Autor(a) principal: Dias, Albiane Carvalho
Data de Publicação: 2021
Tipo de documento: Tese
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
Texto Completo: https://tede.ufrrj.br/jspui/handle/jspui/6074
Resumo: Maize (Zea mays L) is one of the world?s leading produced cereals. Its inoculation with diazotrophic bacteria described as plant growth-promoters can reduce the demand for nitrogen fertilizers and at the same time the costs associated with this practice. This study investigated how inoculation of the diazotrophic bacteria Azospirillum brasilense, Azospirillum baldaniorum and Herbaspirillum seropedicae in maize plants would influence the modulation of root architecture and the parameters associated with the absorption rate of nutrients, especially nitrogen (N), under different conditions of availability of N. Experiments were developed in a hydroponic system and sterile substrate (sand+vermiculite) in a greenhouse, in order to verify the metabolism and expression of genes involved in the absorption and assimilation of N. For this purpose, nitrogen fractions, the activity of the enzymes nitrate reductase and glutamine synthetase, the gene expression of proton pump isoforms (ZmHA2 and ZmHA4), high-affinity nitrate transporters (NO3-) (ZmNRT2.3, ZmNRT2.5 and ZmNRT3.1A) and N-reduction and assimilation enzymes (ZmNR1, ZmNR2, ZmGS1.1, ZmGS1.5, ZmGS2 and ZmNADH-GOGAT) were evaluated. Parameters such as biomass accumulation, total N, N use efficiency (NUE), leaf area and chlorophyll index were also evaluated. The inoculation with A. baldaniorum (Ab)-Sp245 and H. seropedicae (Hs)-ZAE94 strains promoted the greatest changes in root architecture, with improvement in all parameters evaluated, which was in agreement with the increase in dry mass and accumulation of N, but they were dependent on the date of sampling and experiment. Increases in the accumulation of biomass and total N of up to 150 and 180%, respectively, were found in the treatment inoculated with Ab-Sp245 in relation to the control. Inoculation with both strains also promoted increments greater than 90% for length, volume and root ?rea. The parameters improved due to greater availability of N. The Ab-Sp245 was more effective in the greater absorption of macronutrients, with higher speed of absorption of NO3- and provided a greater NUE under low N. The changes caused by inoculation in the gene expression profile were less consistent, only after 2 h of induction of the NO3- transport system did Hs-ZAE94 increase the expression of two high-affinity NO3- transporters (ZmNRT2.3 and ZmNRT2.5) and two isoforms of glutamine synthetase (ZmGS1.1 and ZmGS2) When compared to the control, but those found in the content of soluble metabolites and enzymatic activity indicated an improvement in the N assimilation process in the inoculated plants. The shift in root architecture and the maintenance of N uptake per unit of root area through inoculation with Ab-Sp245 was probably the main effect.
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spelling Reis, Veronica Massena631.052.847-53http://lattes.cnpq.br/9099587982889283Santos, Leandro AzevedoReis, Veronica MassenaAlves, Bruno Jos? RodriguesCoelho, Irene da SilvaVidal, Marcia SoaresAlves, Gabriela Cavalcanti142.182.367-55http://lattes.cnpq.br/7875627543551810Dias, Albiane Carvalho2022-10-17T19:32:21Z2021-11-30DIAS, Albiane Carvalho. Metabolismo de nitrog?nio em plantas de milho inoculadas com dois g?neros de bact?rias diazotr?ficas. 2021. 134 f. Tese (Doutorado em Agronomia, Ci?ncia do Solo) - Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2021.https://tede.ufrrj.br/jspui/handle/jspui/6074Maize (Zea mays L) is one of the world?s leading produced cereals. Its inoculation with diazotrophic bacteria described as plant growth-promoters can reduce the demand for nitrogen fertilizers and at the same time the costs associated with this practice. This study investigated how inoculation of the diazotrophic bacteria Azospirillum brasilense, Azospirillum baldaniorum and Herbaspirillum seropedicae in maize plants would influence the modulation of root architecture and the parameters associated with the absorption rate of nutrients, especially nitrogen (N), under different conditions of availability of N. Experiments were developed in a hydroponic system and sterile substrate (sand+vermiculite) in a greenhouse, in order to verify the metabolism and expression of genes involved in the absorption and assimilation of N. For this purpose, nitrogen fractions, the activity of the enzymes nitrate reductase and glutamine synthetase, the gene expression of proton pump isoforms (ZmHA2 and ZmHA4), high-affinity nitrate transporters (NO3-) (ZmNRT2.3, ZmNRT2.5 and ZmNRT3.1A) and N-reduction and assimilation enzymes (ZmNR1, ZmNR2, ZmGS1.1, ZmGS1.5, ZmGS2 and ZmNADH-GOGAT) were evaluated. Parameters such as biomass accumulation, total N, N use efficiency (NUE), leaf area and chlorophyll index were also evaluated. The inoculation with A. baldaniorum (Ab)-Sp245 and H. seropedicae (Hs)-ZAE94 strains promoted the greatest changes in root architecture, with improvement in all parameters evaluated, which was in agreement with the increase in dry mass and accumulation of N, but they were dependent on the date of sampling and experiment. Increases in the accumulation of biomass and total N of up to 150 and 180%, respectively, were found in the treatment inoculated with Ab-Sp245 in relation to the control. Inoculation with both strains also promoted increments greater than 90% for length, volume and root ?rea. The parameters improved due to greater availability of N. The Ab-Sp245 was more effective in the greater absorption of macronutrients, with higher speed of absorption of NO3- and provided a greater NUE under low N. The changes caused by inoculation in the gene expression profile were less consistent, only after 2 h of induction of the NO3- transport system did Hs-ZAE94 increase the expression of two high-affinity NO3- transporters (ZmNRT2.3 and ZmNRT2.5) and two isoforms of glutamine synthetase (ZmGS1.1 and ZmGS2) When compared to the control, but those found in the content of soluble metabolites and enzymatic activity indicated an improvement in the N assimilation process in the inoculated plants. The shift in root architecture and the maintenance of N uptake per unit of root area through inoculation with Ab-Sp245 was probably the main effect.O milho (Zea mays L.) ? um dos principais cereais produzidos no mundo. A sua inocula??o com bact?rias diazotr?ficas descritas como promotoras de crescimento de plantas pode reduzir a demanda por fertilizantes nitrogenados e ao mesmo tempo os gastos associados a essa pr?tica. Este estudo investigou como a inocula??o das bact?rias diazotr?ficas Azospirillum brasilense, Azospirillum baldaniorum e Herbaspirillum seropedicae em plantas de milho influenciaria a modula??o da arquitetura da raiz e os par?metros associados ? taxa de absor??o de nutrientes, especialmente de nitrog?nio (N), sob diferentes condi??es de disponibilidade de N. Foram desenvolvidos seis experimentos, quatro em sistema hidrop?nico e dois em substrato est?ril (areia+vermiculita) em casa de vegeta??o, a fim de verificar o metabolismo e a express?o de genes envolvidos na absor??o e assimila??o de N. Para isso, foram avaliadas nos tecidos vegetais as fra??es nitrogenadas, a??cares sol?veis, a atividade das enzimas nitrato redutase e glutamina sintetase, a express?o g?nica de isoformas de bombas de pr?tons (ZmHA2 e ZmHA4), transportadores de alta afinidade de nitrato (NO3-) (ZmNRT2.3, ZmNRT2.5 e ZmNRT3.1A) e das enzimas de redu??o e assimila??o de N (ZmNR1, ZmNR2, ZmGS1.1, ZmGS1.5, ZmGS2 e ZmNADH-GOGAT). Par?metros como ac?mulo de biomassa, N total, efici?ncia do uso de N (EUN), ?rea foliar e ?ndice de clorofila tamb?m foram avaliados. A inocula??o com as estirpes de Azospirillum baldaniorum (Ab)-Sp245 e Herbaspirillum seropedicae (Hs)-ZAE94 promoveram as maiores mudan?as na arquitetura radicular, com melhora em todos os par?metros avaliados, o que estava de acordo com o aumento da massa seca e ac?mulo de N, mas dependente da data de amostragem e do ensaio. Incrementos no ac?mulo de biomassa e N total em at? 150 e 180%, respectivamente, foram encontrados no tratamento inoculado com Ab-Sp245 em rela??o ao controle. A inocula??o com ambas as estirpes tamb?m promoveu incrementos superiores a 90% para comprimento, volume e ?rea radicular. Os par?metros avaliados melhoraram em fun??o da maior disponibilidade de N. A Ab-Sp245 foi mais efetiva na maior absor??o de macronutrientes, com maior velocidade de absor??o de NO3- e proporcionou uma maior EUN sob baixo N. As mudan?as provocadas pela inocula??o no perfil de express?o g?nica foram menos consistentes, somente ap?s 2 h da indu??o do sistema de transporte de NO3- a Hs-ZAE94 aumentou a express?o de dois transportadores de alta afinidade de NO3- (ZmNRT2.3 e ZmNRT2.5) e duas isoformas de glutamina sintetase (ZmGS1.1 e ZmGS2) quando comparado ao controle, mas as encontradas no conte?do de metab?litos sol?veis e atividade enzim?tica indicaram melhoria no processo de assimila??o de N nas plantas inoculadas. As mudan?as na arquitetura radicular e a manuten??o da absor??o de N por unidade de ?rea da raiz atrav?s da inocula??o com Ab-Sp245 foi provavelmente o efeito principal.Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2022-10-17T19:32:19Z No. of bitstreams: 1 2021 - Albiane Carvalho Dias.pdf: 3382057 bytes, checksum: fcb9be6d660752a123aeb2b954aad3e6 (MD5)Made available in DSpace on 2022-10-17T19:32:21Z (GMT). No. of bitstreams: 1 2021 - Albiane Carvalho Dias.pdf: 3382057 bytes, checksum: fcb9be6d660752a123aeb2b954aad3e6 (MD5) Previous issue date: 2021-11-30CAPES - Coordena??o de Aperfei?oamento de Pessoal de N?vel SuperiorCNPq - Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gicoFAPERJ - Funda??o Carlos Chagas Filho de Amparo ? Pesquisa do Estado do Rio de JaneiroEMBRAPA - Empresa Brasileira de Pesquisa Agropecu?riaapplication/pdfhttps://tede.ufrrj.br/retrieve/71083/2021%20-%20Albiane%20Carvalho%20Dias.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Agronomia - Ci?ncia do SoloUFRRJBrasilInstituto de AgronomiaADESEMOYER, A. O.; KLOEPPER, J. W. Plant-microbes interactions in enhanced fertilizeruse efficiency. Applied Microbiology and Biotechnlogy, v. 85, n. 1, p. 1-12, 2009. ADESEMOYE, A.O.; TORBERT, H.A.; KLOEPPER J.W. Plant growth promoting rhizobacteria allow reduced application rates of chemical fertilizers. 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dc.title.por.fl_str_mv Metabolismo de nitrog?nio em plantas de milho inoculadas com dois g?neros de bact?rias diazotr?ficas
dc.title.alternative.eng.fl_str_mv Nitrogen metabolism in maize plants inoculated with two genera of diazotrophic bacteria
title Metabolismo de nitrog?nio em plantas de milho inoculadas com dois g?neros de bact?rias diazotr?ficas
spellingShingle Metabolismo de nitrog?nio em plantas de milho inoculadas com dois g?neros de bact?rias diazotr?ficas
Dias, Albiane Carvalho
Azospirillum baldaniorum
Bact?ria promotora de crescimento de planta
Herbaspirillum seropedicae
Nitrato
Zea mays L.
Plant growth-promoting bacteria
Nitrate
Agronomia
title_short Metabolismo de nitrog?nio em plantas de milho inoculadas com dois g?neros de bact?rias diazotr?ficas
title_full Metabolismo de nitrog?nio em plantas de milho inoculadas com dois g?neros de bact?rias diazotr?ficas
title_fullStr Metabolismo de nitrog?nio em plantas de milho inoculadas com dois g?neros de bact?rias diazotr?ficas
title_full_unstemmed Metabolismo de nitrog?nio em plantas de milho inoculadas com dois g?neros de bact?rias diazotr?ficas
title_sort Metabolismo de nitrog?nio em plantas de milho inoculadas com dois g?neros de bact?rias diazotr?ficas
author Dias, Albiane Carvalho
author_facet Dias, Albiane Carvalho
author_role author
dc.contributor.advisor1.fl_str_mv Reis, Veronica Massena
dc.contributor.advisor1ID.fl_str_mv 631.052.847-53
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/9099587982889283
dc.contributor.advisor-co1.fl_str_mv Santos, Leandro Azevedo
dc.contributor.referee1.fl_str_mv Reis, Veronica Massena
dc.contributor.referee2.fl_str_mv Alves, Bruno Jos? Rodrigues
dc.contributor.referee3.fl_str_mv Coelho, Irene da Silva
dc.contributor.referee4.fl_str_mv Vidal, Marcia Soares
dc.contributor.referee5.fl_str_mv Alves, Gabriela Cavalcanti
dc.contributor.authorID.fl_str_mv 142.182.367-55
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/7875627543551810
dc.contributor.author.fl_str_mv Dias, Albiane Carvalho
contributor_str_mv Reis, Veronica Massena
Santos, Leandro Azevedo
Reis, Veronica Massena
Alves, Bruno Jos? Rodrigues
Coelho, Irene da Silva
Vidal, Marcia Soares
Alves, Gabriela Cavalcanti
dc.subject.por.fl_str_mv Azospirillum baldaniorum
Bact?ria promotora de crescimento de planta
Herbaspirillum seropedicae
Nitrato
Zea mays L.
topic Azospirillum baldaniorum
Bact?ria promotora de crescimento de planta
Herbaspirillum seropedicae
Nitrato
Zea mays L.
Plant growth-promoting bacteria
Nitrate
Agronomia
dc.subject.eng.fl_str_mv Plant growth-promoting bacteria
Nitrate
dc.subject.cnpq.fl_str_mv Agronomia
description Maize (Zea mays L) is one of the world?s leading produced cereals. Its inoculation with diazotrophic bacteria described as plant growth-promoters can reduce the demand for nitrogen fertilizers and at the same time the costs associated with this practice. This study investigated how inoculation of the diazotrophic bacteria Azospirillum brasilense, Azospirillum baldaniorum and Herbaspirillum seropedicae in maize plants would influence the modulation of root architecture and the parameters associated with the absorption rate of nutrients, especially nitrogen (N), under different conditions of availability of N. Experiments were developed in a hydroponic system and sterile substrate (sand+vermiculite) in a greenhouse, in order to verify the metabolism and expression of genes involved in the absorption and assimilation of N. For this purpose, nitrogen fractions, the activity of the enzymes nitrate reductase and glutamine synthetase, the gene expression of proton pump isoforms (ZmHA2 and ZmHA4), high-affinity nitrate transporters (NO3-) (ZmNRT2.3, ZmNRT2.5 and ZmNRT3.1A) and N-reduction and assimilation enzymes (ZmNR1, ZmNR2, ZmGS1.1, ZmGS1.5, ZmGS2 and ZmNADH-GOGAT) were evaluated. Parameters such as biomass accumulation, total N, N use efficiency (NUE), leaf area and chlorophyll index were also evaluated. The inoculation with A. baldaniorum (Ab)-Sp245 and H. seropedicae (Hs)-ZAE94 strains promoted the greatest changes in root architecture, with improvement in all parameters evaluated, which was in agreement with the increase in dry mass and accumulation of N, but they were dependent on the date of sampling and experiment. Increases in the accumulation of biomass and total N of up to 150 and 180%, respectively, were found in the treatment inoculated with Ab-Sp245 in relation to the control. Inoculation with both strains also promoted increments greater than 90% for length, volume and root ?rea. The parameters improved due to greater availability of N. The Ab-Sp245 was more effective in the greater absorption of macronutrients, with higher speed of absorption of NO3- and provided a greater NUE under low N. The changes caused by inoculation in the gene expression profile were less consistent, only after 2 h of induction of the NO3- transport system did Hs-ZAE94 increase the expression of two high-affinity NO3- transporters (ZmNRT2.3 and ZmNRT2.5) and two isoforms of glutamine synthetase (ZmGS1.1 and ZmGS2) When compared to the control, but those found in the content of soluble metabolites and enzymatic activity indicated an improvement in the N assimilation process in the inoculated plants. The shift in root architecture and the maintenance of N uptake per unit of root area through inoculation with Ab-Sp245 was probably the main effect.
publishDate 2021
dc.date.issued.fl_str_mv 2021-11-30
dc.date.accessioned.fl_str_mv 2022-10-17T19:32:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv DIAS, Albiane Carvalho. Metabolismo de nitrog?nio em plantas de milho inoculadas com dois g?neros de bact?rias diazotr?ficas. 2021. 134 f. Tese (Doutorado em Agronomia, Ci?ncia do Solo) - Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2021.
dc.identifier.uri.fl_str_mv https://tede.ufrrj.br/jspui/handle/jspui/6074
identifier_str_mv DIAS, Albiane Carvalho. Metabolismo de nitrog?nio em plantas de milho inoculadas com dois g?neros de bact?rias diazotr?ficas. 2021. 134 f. Tese (Doutorado em Agronomia, Ci?ncia do Solo) - Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2021.
url https://tede.ufrrj.br/jspui/handle/jspui/6074
dc.language.iso.fl_str_mv por
language por
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