Influ?ncia da densidade populacional de larvas no conte?do de triacilglicerol, fecundidade e tamanho de f?meas em Aedes aegypti (Linnaeus) (D?ptera: Culicidae)

Detalhes bibliográficos
Autor(a) principal: Silva, Elaine Rodrigues Miranda Nery da
Data de Publicação: 2020
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
Texto Completo: https://tede.ufrrj.br/jspui/handle/jspui/6129
Resumo: Given the importance of the Aedes aegypti as arboviroses vector, understanding the impact of abiotic factors, such as population density in the larval rearing s, during larval development, is fundamental to improve control methods. The aims of this work were to analyze the effects of population density on the triacylglycerol (TAG) reserves, fecundity and the size of mosquitos. To evaluate the influence of density on the amount of triacylglycerol in the different stages of development of A. aegypti, the larvae were reared in the following densities 0.04, 0.32 and 0.8 larvae / ml, represented by D1, D2 and D3, respectively. It was observed that different larval rearing affected the amount of triacylglycerol on the different development stages of this insect. The group reared in a high population density (D3) presented greater storage of triacylglycerol, except when analyzing the amount of triacylglycerol ?g/protein of fat body in blood-fed females. In addition, it was possible to observe a higher resistance to fasting, higher amount of laid eggs. Insects reared on D2 and D3 were larger than D1. This study provided interesting data for future investigations as to how densitiy of larval rearing affect the lipid metabolism concerning to synthesis and mobilization of triacylglycerol store in A. aegypti and how does it influences it size and the reproduction.
id UFRRJ-1_28370c42e1784b1e38155b239f13d7a7
oai_identifier_str oai:localhost:jspui/6129
network_acronym_str UFRRJ-1
network_name_str Biblioteca Digital de Teses e Dissertações da UFRRJ
repository_id_str
spelling Pontes, Emerson Guedes045.534.107-96http://lattes.cnpq.br/1562085358907265Pontes, Emerson GuedesMallet, Jacenir Reis dos SantosOliveira, Marise Maleck deSilva, Julia dos Santos147.655.907-47http://lattes.cnpq.br/6683860713209935Silva, Elaine Rodrigues Miranda Nery da2022-12-12T20:28:50Z2020-11-30SILVA, Elaine Rodrigues Miranda Nery da. Influ?ncia da densidade populacional de larvas no conte?do de triacilglicerol, fecundidade e tamanho de f?meas em Aedes aegypti (Linnaeus) (D?ptera: Culicidae). 2020. 41 f. Disserta??o (Mestrado em Biologia Animal) - Instituto de Ci?ncias Biol?gicas e da Sa?de, Universidade Federal Rural do Rio de Janeiro, Serop?dica, RJ. 2020.https://tede.ufrrj.br/jspui/handle/jspui/6129Given the importance of the Aedes aegypti as arboviroses vector, understanding the impact of abiotic factors, such as population density in the larval rearing s, during larval development, is fundamental to improve control methods. The aims of this work were to analyze the effects of population density on the triacylglycerol (TAG) reserves, fecundity and the size of mosquitos. To evaluate the influence of density on the amount of triacylglycerol in the different stages of development of A. aegypti, the larvae were reared in the following densities 0.04, 0.32 and 0.8 larvae / ml, represented by D1, D2 and D3, respectively. It was observed that different larval rearing affected the amount of triacylglycerol on the different development stages of this insect. The group reared in a high population density (D3) presented greater storage of triacylglycerol, except when analyzing the amount of triacylglycerol ?g/protein of fat body in blood-fed females. In addition, it was possible to observe a higher resistance to fasting, higher amount of laid eggs. Insects reared on D2 and D3 were larger than D1. This study provided interesting data for future investigations as to how densitiy of larval rearing affect the lipid metabolism concerning to synthesis and mobilization of triacylglycerol store in A. aegypti and how does it influences it size and the reproduction.Dada a import?ncia do mosquito Aedes aegypti como vetor de arboviroses emergentes, ? fundamental compreender o impacto de fatores abi?ticos, como a densidade populacional na cria??o larval durante o seu desenvolvimento, para melhorar os m?todos de controle. Os objetivos deste trabalho foram analisar os efeitos da densidade populacional nas reservas de triacilglicerol (TAG), fecundidade e tamanho dos mosquitos. Para avaliar a influ?ncia da densidade na quantidade de triacilglicerol nos diferentes est?gios de desenvolvimento de A. aegypti, as larvas foram criadas nas seguintes densidades: 0,04, 0,32 e 0,8 larva / ml, representadas por D1, D2 e D3, respectivamente. Observou-se que nas diferentes cria??es larvais a quantidade de triacilglicerol, nos diferentes est?gios de desenvolvimento desse inseto, foi afetada. O grupo criado em alta densidade populacional (D3) apresentou maior armazenamento de triacilglicerol, exceto ao analisar a quantidade de triacilglicerol ?g / prote?na do corpo gordo em f?meas alimentadas com sangue. Al?m disso, foi poss?vel observar maior resist?ncia ao jejum, maior quantidade de ovos postos e que os insetos criados em D2 e D3 eram maiores que em D1. Este estudo forneceu dados que contribuir?o para futuras investiga??es sobre como a densidade da cria??o larval afeta o metabolismo lip?dico em rela??o ? s?ntese e mobiliza??o do estoque de triacilglicerol em A. aegypti e como isso influencia seu tamanho e a reprodu??o.Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2022-12-12T20:28:50Z No. of bitstreams: 1 2020 - Elaine Rodrigues Miranda Nery da Silva.pdf: 1037971 bytes, checksum: 920ba00caa550ba2451864808c3f175c (MD5)Made available in DSpace on 2022-12-12T20:28:50Z (GMT). No. of bitstreams: 1 2020 - Elaine Rodrigues Miranda Nery da Silva.pdf: 1037971 bytes, checksum: 920ba00caa550ba2451864808c3f175c (MD5) Previous issue date: 2020-11-30CAPES - Coordena??o de Aperfei?oamento de Pessoal de N?vel Superiorapplication/pdfhttps://tede.ufrrj.br/retrieve/71356/2020%20-%20Elaine%20Rodrigues%20Miranda%20Nery%20da%20Silva.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Biologia AnimalUFRRJBrasilInstituto de Ci?ncias Biol?gicas e da Sa?deALTO B. W.; MUT, E. J AND LAMPMAN, R. L. Effects of nutrition and density in Culex pipiens. Medical and Veterinary Entomology, v. 7200, p. 396?406. 2012. ARRESE, E. L.; SOULAGES, J. L. Insect fat body: energy, metabolism, and regulation. Annual Review of Entomology, v. 55, n. 87, p. 207?225. 2010. BALDAL, E. A.; LINDE, K. VAN DER; ALPHEN, J. J. M. VAN. The effects of larval density on adult life-history traits in three species of Drosophila. Mechanisms of Ageing and Development 126 v. 126, p. 407?416. 2005. BALEBA, S. B. S., MASIGA, D., TORTO, B., WELDON, C. W., & GETAHUN, M. N.. Effect of larval density and substrate quality on the wing geometry of Stomoxys calcitrans L. (Diptera: Muscidae). Parasites & Vectors, 12(1), 222. 2019. BARLETTA, A. B. F. et al. Emerging role of lipid droplets in Aedes aegypti immune response against bacteria and Dengue virus. Scientific Reports, v. 6, n. September 2015, p. 1?13. 2016. BESERRA, E. B.; FERNANDES, C. R. M.; RIBEIRO, P. S. Rela??o entre densidade larval e ciclo de vida, tamanho e fecundidade de Aedes (Stegomyia) aegypti (l.) (Diptera: Culicidae) em laborat?rio. Neotropical Entomology, v. 38, n. 6, p. 847?852. 2009. BORASH, D. J.; HO, G. T. Patterns of selection: Stress resistance and energy storage in density-dependent populations of Drosophila melanogaster. Journal of Insect Physiology, v. 47, n. 12, p. 1349?1356. 2001. BRIEGEL, H. Metabolic relationship between female body size, reserves, and fecondity of Aedes aegypti. J.Insect.Physiol., v. 36, n. 3, p. 165?172. 1990. BRIEGEL, H. Physiological bases of mosquito ecology. Journal of Vector Ecology. p. 1?11. 2003. BRIEGEL, H.; HEFTI, M.; DIMARCO, E. Lipid metabolism during sequential gonotrophic cycles in large and small female Aedes aegypti. Journal of Insect Physiology.v. 48, p. 547?554. 2002. BROWN, J. E.; EVANS, B. R.; ZHENG, W.; OBAS, V.; MARTINEZ, L. B.; EGIZI, A.; ZHAO, H.; CACCONE, A.; POWELL, J. R.et al. Human impacts have shaped historical and recent evolution in Aedes aegypti , the dengue and yellow fever mosquito. The Society for the Study of Evolution . p. 514?525. 2013. CHUNG, H.-N. et al. Fat Body Organ Culture System in Aedes Aegypti, a Vector of Zika Virus. Journal of Visualized Experiments, n. 126, p. 1?8. 2017. CLIFTON, M. E.; NORIEGA, F. G. The fate of follicles after a blood meal is dependent on previtellogenic nutrition and juvenile hormone in Aedes aegypti. Journal of Insect Physiology, v. 58, n. 7, p. 1007?1019. 2012. COSTA, F. S. et al. Din?mica populacional de Aedes aegypti (L) em ?rea urbana de alta incid?ncia de dengue. Revista da Sociedade Brasileira de Medicina Tropical, v. 41, n. 3, p. 309?312. 2008. COURET, J.; BENEDICT, M. Q. A meta-analysis of the factors influencing development rate variation in Aedes aegypti ( Diptera : Culicidae ). BMC Ecology, 2014,p. 1?15. 2014. COURET, J.; DOTSON, E.; BENEDICT, M. Q. Temperature, larval diet, and density effects on development rate and survival of Aedes aegypti (Diptera: Culicidae). PLOS ONE, 9(2). 2014. DICKENS, B. L. et al. Determining environmental and anthropogenic factors which explain the global distribution of Aedes aegypti and Ae. albopictus. BMJ Global Health, v. 3, n. 4, p. 2018. FARNESI, L. C. et al. Darker eggs of mosquitoes resist more to dry conditions: Melanin enhances serosal cuticle contribution in egg resistance to desiccation in Aedes, Anopheles and Culex vectors. PLoS Neglected Tropical Diseases, v. 11, n. 10, p. 1? 20. 2017. FARNESI, L. C. et al. The influence of a light and dark cycle on the egg laying activity of Aedes aegypti ( Linnaeus , 1762 ) ( Diptera : Culicidae ). Mem Inst Oswaldo Cruz. v. 113, n. 4, p. 4?9. 2018. FIOCRUZ. Dengue. Instru??es para Pessoal de Combate ao Vetor. Manual de Normas T?nicas. FUNASA. 2001. ATTARDO, G. M.; HANSEN, I. A. S. H. S.; RAIKHEL, A. S Identification of two cationic amino acid transporters required for nutritional signaling during mosquito reproduction. Journal of Experimental Biology, v. 209, n. 16, p. 3071?3078. 2006. GIBBONS, G. F.; ISLAM, K.; PEASE, R. J. Mobilisation of triacylglycerol stores. Biochimica et biophysica acta v. 1483. 2000. GILBERT, L. I.; CHINO, H. Transport of lipids in insects. J. Lipid Res., v. 15, n. 5, p. 439? 456, set. 1974. GILLES, J. R. L. et al. Density-Dependent Effects in Experimental Larval Populations of Anopheles arabiensis (Diptera: Culicidae) Can Be Negative, Neutral, or Overcompensatory Depending on Density and Diet Levels. Journal of Medical Entomology, v. 48, n. 2, p. 296?304. 2011. GONDIM, K; ATELLA, G, PONTES, E; MAJEROWICZ, D. Lipid metabolism in insect disease vectors. Insect Biochemistry and Molecular Biology, 101, 108?123. 2018. HEINISCH E SILVA, M. R. et al. Seasonal and spatial distribution of Aedes aegypti and Aedes albopictus in a municipal urban park in S?o Paulo, SP, Brazil. Acta Tropica. 2018. HURD, H. Manipulation of medically important insect vectors by their parasites. Annual Review of Entomology, v. 48, n. 1, p. 141?161, 2003. IMAM, H.; SOFI, G.; AZIZ, S. The basic rules and methods of mosquito rearing (Aedes aegypti). Tropical Parasitology. v. 4, n. 1, p. 53?55. 2014. JIRAKANJANAKIT, N. et al. Influence of larval density or food variation on the geometry of the wing of Aedes ( Stegomyia ) aegypti. Tropical Medicine and International Health. volume v. 12, n. 11, p. 1354?1360. 2007. JUNJHON, J. et al. Ultrastructural characterization and three-dimensional architecture of replication sites in dengue virus-infected mosquito cells. Journal of Virology. 88, 4687?4697. 2014. KABA, D., BERTE, D., Ta Bi Tra, D., TELLER? ?a, J., SOLANO, P., DUJARDIN, J.-P., The wing venation patterns to identify single tsetse flies. 2016. doi:10.1016/j.meegid.2016.10.008 KANTOR, I. N. Dengue, Zika and Chikungunya. Medicina, p. 1?5. 2016. KRAEMER, M. U. G. et al. The global distribution of the arbovirus vectors Aedes aegypti and Ae . albopictus. p. 1?18. 2015. doi: 10.7554/eLife.08347 LEHMANN, T.. DALTON, R; KIM, E. H.; DAHL, E.; DIABATE, A.; DABIRE, R; DUJARDIN, J. P. Genetic contribution to variation in larval development time , adult size , and longevity of starved adults of Anopheles gambiae. Infection, Genetics and Evolution. v. 6, p. 410?416. 2006. LING, L.; KOKOZA, A. V.; ZHANG, C.; ARKZOY, E.; RAIKHEL, A. S. MicroRNA-277 targets insulin-like peptides 7 and 8 to control lipid metabolism and reproduction in Aedes aegypti mosquitoes. Proceedings of the National Academy of Sciences, v. 114, n. 38, p. E8017?E8024. 2017. LONG, K. C. et al. Experimental Transmission of Mayaro Virus by Aedes aegypti. v. 85, n. 4, p. 750?757. 2011. doi: https://doi.org/10.4269/ajtmh.2011.11-0359 LORENZ, C.; ALMEIDA, F.; LOPES, F. A.; LOUISE, C; PEREIRA, S. N.; PETERSEN, V.; VIDAL, P. O.; SUESDEK , F. V. L.; Geometric morphometrics in mosquitoes: What has been measured?. Infection, Genetics and Evolution. 2017. doi: 10.1016/j.meegid.2017.06.029 LOUNIBOS, L P;NISHIMURA, N; CONN, J;OLIVEIRA, R. Life history correlates of adult size in the Malaria Vector Anopheles darlingi. M?m?rias Instituto Oswaldo Cruz, vol 90 (6): 769 - 774. 1995. MARTINS, R. et al. Hypometabolic strategy and glucose metabolism maintenance of Aedes aegypti egg desiccation. Comparative Biochemistry and Physiology, Part B, p. 2018. MENDES, A. M. et al. Conserved mosquito/parasite interactions affect development of Plasmodium falciparum in Africa. PLoS Pathogens, v. 4, n. 5. 2008. MOORE, C. G AND FISHER, B. R. Competition in Mosquitoes. Density and Secies Ratio Effects on Gronwth, Mortaity, Fecundity, and Production of Growth Retardant. Annals of the entomological society of America, v. 62, n. 6. 1969. MOSTOWY, W. M.; FOSTER, W. A. Antagonistic effects of energy status on meal size and egg-batch size of Aedes aegypti ( Diptera : Culicidae ). Journal of Vector Ecology. p. 84?93. 2004. NATTERO, J.; PICCINALI, R. V.; LOPES, C. M.; HERN?NDEZ, M. L.; ABRAHAN, L.; LOBBIA, P. A.; RODR?GUEZ, C. S.; FUENTE, A. L. C. Morphometric variability among the species of the Sordida subcomplex ( Hemiptera : Reduviidae : Triatominae ): evidence for differentiation across the distribution range of Triatoma sordida. Parasites & Vectors p. 1?14. 2017. PERERA R, RILEY C, ISAAC G, HOPF-JANNASCH AS, MOORE RJ, et al. Dengue Virus Infection Perturbs Lipid Homeostasis in Infected Mosquito Cells. PLoS Pathogens 8(3): e1002584. 2012. doi:10.1371/journal.ppat.1002584 PETERSON, G. L. A Simplification of the Protein Assay Method of Lowry et al . Analytical Biochemistry. Which is More Generally Applicable. v. 356, p. 346?356. 1977. PRICE, D. P. et al. Small mosquitoes, large implications: crowding and starvation affects gene expression and nutrient accumulation in Aedes aegypti. Parasites & vectors, v. 8, n. 1, p. 252. 2015. QUARESMA, M. A. Avalia??o da implanta??o do componente controle vetorial do Programa de Controle da Dengue em Porto Seguro-BA/Brasil. [s.l.] FIOCRUZ. 2017. REZENDE, G. L. et al. Embryonic desiccation resistance in Aedes aegypti: Presumptive role of the chitinized Serosal Cuticle. BMC Developmental Biology, v. 8, p. 1?14. 2008. ROWLAND, M.; BOERSMA, E. Changes in the spontaneous flight activity of the mosquito Anopheles stephensi by parasitization with the rodent malaria Plasmodium yoelii. Parasitology, v. 97, n. 2, p. 221?227. 1988. ROY, S. et al. Regulation of Reproductive Processes in Female Mosquitoes. In: Advances in Insect Physiology. [s.l: s.n.]. v. 51p. 115?144. 2016 SANTOS, C.; LEITE, G.; FALQUETO, A. Does native bromeliads represent important breeding sites for Aedes aegypti (L.) (Diptera: Culicidae) in urbanized areas? Neotropical Entomology, v. 40, n. 2, p. 278?281, 2011. SOULAGES, J. L.; ARRESE. E. L. Insect fat body: energy, metabolism, and regulation. Annu Rev Entomol. 55: 207?225. 2011. doi:10.1146/annurev-ento-112408-085356. TELANG, A. AND WELLS, M. A. The effect of larval and adult nutrition on successful autogenous egg production by a mosquito. Journal of Insect Physiology. v. 50, p. 677? 685. 2004. TELANG, A.; FRAME, L.; BROWN, M. R. Larval feeding duration affects ecdysteroid levels and nutritional reserves regulating pupal commitment in the yellow fever mosquito Aedes aegypti ( Diptera : Culicidae ). The Journal of Experimental Biology. p. 854? 864. 2007. WANG, X. et al. Hormone and receptor interplay in the regulation of mosquito lipid metabolism. PNAS. e2709?e2718. 2017. WEAVER, S. C. COSTA, F., et al. Zika Virus: History, Emergence, Biology, and Prospects for Control. Antiviral research. p. 69?80. 2017. WILKE, A. B. B. et al. Ornamental bromeliads of Miami-Dade County, Florida are important breeding sites for Aedes aegypti (Diptera: Culicidae). Parasites & Vectors, v. 11, n. 1, p. 283. 2018. YOSHIOKA, M. et al. Diet and density dependent competition affect larval performance and oviposition site selection in the mosquito species Aedes albopictus (Diptera : Culicidae). Parasites & Vectors. p. 1?11. 2012. ZARA, L. S. A. SANTOS, M.; OLIVEIRA, S. F. Estrat?gias de controle do Aedes aegypti: uma revis?o. Epidemiologia e Servi?os de Sa?de, v. 25, n. 2, p. 1?2, 2016. ZHOU, G.; PENNINGTON, J. E.; ?, M. A. W. Utilization of pre-existing energy stores of female Aedes aegypti mosquitoes during the first gonotrophic cycle. Insect Biochemistry and Molecular Biology, v. 34, p. 919?925, 2004. ZIEGLER, R.; ANTWERPEN, R. VAN. Lipid uptake by insect oocytes. Insect Biochemistry and Molecular Biology. 36 (2006) 264?272. 2006. SECRETARIA DE ESTADO DE SA?DE DO RIO DE JANEIRO. Boletim epidemiol?gico arboviroses. N? 004. n. 85, p. 1?17. 2018. ZIRBEL, K.; EASTMOND, B.; ALTO, B. W. Parental and offspring larval diets interact to influence life-history traits and infection with dengue virus in Aedes aegypti. R. Soc. open sci. 5: 180539. 2018.Aedes aegyptitriacilgliceroldensidademosquitostriacylglyceroldensitymosquitoesBioqu?micaInflu?ncia da densidade populacional de larvas no conte?do de triacilglicerol, fecundidade e tamanho de f?meas em Aedes aegypti (Linnaeus) (D?ptera: Culicidae)Influence of the population density of larvae on the content of triacylglycerol, fecundity and size of females in Aedes aegypti (Linnaeus) (D?ptera: Culicidae)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRRJinstname:Universidade Federal Rural do Rio de Janeiro (UFRRJ)instacron:UFRRJTHUMBNAIL2020 - Elaine Rodrigues Miranda Nery da Silva.pdf.jpg2020 - Elaine Rodrigues Miranda Nery da Silva.pdf.jpgimage/jpeg1943http://localhost:8080/tede/bitstream/jspui/6129/4/2020+-+Elaine+Rodrigues+Miranda+Nery+da+Silva.pdf.jpgcc73c4c239a4c332d642ba1e7c7a9fb2MD54TEXT2020 - Elaine Rodrigues Miranda Nery da Silva.pdf.txt2020 - Elaine Rodrigues Miranda Nery da Silva.pdf.txttext/plain84957http://localhost:8080/tede/bitstream/jspui/6129/3/2020+-+Elaine+Rodrigues+Miranda+Nery+da+Silva.pdf.txt9be62f7a51255272ab86ee36e92fceb5MD53ORIGINAL2020 - Elaine Rodrigues Miranda Nery da Silva.pdf2020 - Elaine Rodrigues Miranda Nery da Silva.pdfapplication/pdf1037971http://localhost:8080/tede/bitstream/jspui/6129/2/2020+-+Elaine+Rodrigues+Miranda+Nery+da+Silva.pdf920ba00caa550ba2451864808c3f175cMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82089http://localhost:8080/tede/bitstream/jspui/6129/1/license.txt7b5ba3d2445355f386edab96125d42b7MD51jspui/61292022-12-13 02:00:35.227oai:localhost:jspui/6129Tk9UQTogQ09MT1FVRSBBUVVJIEEgU1VBIFBSP1BSSUEgTElDRU4/QQpFc3RhIGxpY2VuP2EgZGUgZXhlbXBsbyA/IGZvcm5lY2lkYSBhcGVuYXMgcGFyYSBmaW5zIGluZm9ybWF0aXZvcy4KCkxJQ0VOP0EgREUgRElTVFJJQlVJPz9PIE4/Ty1FWENMVVNJVkEKCkNvbSBhIGFwcmVzZW50YT8/byBkZXN0YSBsaWNlbj9hLCB2b2M/IChvIGF1dG9yIChlcykgb3UgbyB0aXR1bGFyIGRvcyBkaXJlaXRvcyBkZSBhdXRvcikgY29uY2VkZSA/IFVuaXZlcnNpZGFkZSAKWFhYIChTaWdsYSBkYSBVbml2ZXJzaWRhZGUpIG8gZGlyZWl0byBuP28tZXhjbHVzaXZvIGRlIHJlcHJvZHV6aXIsICB0cmFkdXppciAoY29uZm9ybWUgZGVmaW5pZG8gYWJhaXhvKSwgZS9vdSAKZGlzdHJpYnVpciBhIHN1YSB0ZXNlIG91IGRpc3NlcnRhPz9vIChpbmNsdWluZG8gbyByZXN1bW8pIHBvciB0b2RvIG8gbXVuZG8gbm8gZm9ybWF0byBpbXByZXNzbyBlIGVsZXRyP25pY28gZSAKZW0gcXVhbHF1ZXIgbWVpbywgaW5jbHVpbmRvIG9zIGZvcm1hdG9zID91ZGlvIG91IHY/ZGVvLgoKVm9jPyBjb25jb3JkYSBxdWUgYSBTaWdsYSBkZSBVbml2ZXJzaWRhZGUgcG9kZSwgc2VtIGFsdGVyYXIgbyBjb250ZT9kbywgdHJhbnNwb3IgYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YT8/byAKcGFyYSBxdWFscXVlciBtZWlvIG91IGZvcm1hdG8gcGFyYSBmaW5zIGRlIHByZXNlcnZhPz9vLgoKVm9jPyB0YW1iP20gY29uY29yZGEgcXVlIGEgU2lnbGEgZGUgVW5pdmVyc2lkYWRlIHBvZGUgbWFudGVyIG1haXMgZGUgdW1hIGM/cGlhIGEgc3VhIHRlc2Ugb3UgCmRpc3NlcnRhPz9vIHBhcmEgZmlucyBkZSBzZWd1cmFuP2EsIGJhY2stdXAgZSBwcmVzZXJ2YT8/by4KClZvYz8gZGVjbGFyYSBxdWUgYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YT8/byA/IG9yaWdpbmFsIGUgcXVlIHZvYz8gdGVtIG8gcG9kZXIgZGUgY29uY2VkZXIgb3MgZGlyZWl0b3MgY29udGlkb3MgCm5lc3RhIGxpY2VuP2EuIFZvYz8gdGFtYj9tIGRlY2xhcmEgcXVlIG8gZGVwP3NpdG8gZGEgc3VhIHRlc2Ugb3UgZGlzc2VydGE/P28gbj9vLCBxdWUgc2VqYSBkZSBzZXUgCmNvbmhlY2ltZW50bywgaW5mcmluZ2UgZGlyZWl0b3MgYXV0b3JhaXMgZGUgbmluZ3U/bS4KCkNhc28gYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YT8/byBjb250ZW5oYSBtYXRlcmlhbCBxdWUgdm9jPyBuP28gcG9zc3VpIGEgdGl0dWxhcmlkYWRlIGRvcyBkaXJlaXRvcyBhdXRvcmFpcywgdm9jPyAKZGVjbGFyYSBxdWUgb2J0ZXZlIGEgcGVybWlzcz9vIGlycmVzdHJpdGEgZG8gZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzIHBhcmEgY29uY2VkZXIgPyBTaWdsYSBkZSBVbml2ZXJzaWRhZGUgCm9zIGRpcmVpdG9zIGFwcmVzZW50YWRvcyBuZXN0YSBsaWNlbj9hLCBlIHF1ZSBlc3NlIG1hdGVyaWFsIGRlIHByb3ByaWVkYWRlIGRlIHRlcmNlaXJvcyBlc3Q/IGNsYXJhbWVudGUgCmlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIG5vIHRleHRvIG91IG5vIGNvbnRlP2RvIGRhIHRlc2Ugb3UgZGlzc2VydGE/P28gb3JhIGRlcG9zaXRhZGEuCgpDQVNPIEEgVEVTRSBPVSBESVNTRVJUQT8/TyBPUkEgREVQT1NJVEFEQSBURU5IQSBTSURPIFJFU1VMVEFETyBERSBVTSBQQVRST0M/TklPIE9VIApBUE9JTyBERSBVTUEgQUc/TkNJQSBERSBGT01FTlRPIE9VIE9VVFJPIE9SR0FOSVNNTyBRVUUgTj9PIFNFSkEgQSBTSUdMQSBERSAKVU5JVkVSU0lEQURFLCBWT0M/IERFQ0xBUkEgUVVFIFJFU1BFSVRPVSBUT0RPUyBFIFFVQUlTUVVFUiBESVJFSVRPUyBERSBSRVZJUz9PIENPTU8gClRBTUI/TSBBUyBERU1BSVMgT0JSSUdBPz9FUyBFWElHSURBUyBQT1IgQ09OVFJBVE8gT1UgQUNPUkRPLgoKQSBTaWdsYSBkZSBVbml2ZXJzaWRhZGUgc2UgY29tcHJvbWV0ZSBhIGlkZW50aWZpY2FyIGNsYXJhbWVudGUgbyBzZXUgbm9tZSAocykgb3UgbyhzKSBub21lKHMpIGRvKHMpIApkZXRlbnRvcihlcykgZG9zIGRpcmVpdG9zIGF1dG9yYWlzIGRhIHRlc2Ugb3UgZGlzc2VydGE/P28sIGUgbj9vIGZhcj8gcXVhbHF1ZXIgYWx0ZXJhPz9vLCBhbD9tIGRhcXVlbGFzIApjb25jZWRpZGFzIHBvciBlc3RhIGxpY2VuP2EuCg==Biblioteca Digital de Teses e Dissertaçõeshttps://tede.ufrrj.br/PUBhttps://tede.ufrrj.br/oai/requestbibliot@ufrrj.br||bibliot@ufrrj.bropendoar:2022-12-13T04:00:35Biblioteca Digital de Teses e Dissertações da UFRRJ - Universidade Federal Rural do Rio de Janeiro (UFRRJ)false
dc.title.por.fl_str_mv Influ?ncia da densidade populacional de larvas no conte?do de triacilglicerol, fecundidade e tamanho de f?meas em Aedes aegypti (Linnaeus) (D?ptera: Culicidae)
dc.title.alternative.eng.fl_str_mv Influence of the population density of larvae on the content of triacylglycerol, fecundity and size of females in Aedes aegypti (Linnaeus) (D?ptera: Culicidae)
title Influ?ncia da densidade populacional de larvas no conte?do de triacilglicerol, fecundidade e tamanho de f?meas em Aedes aegypti (Linnaeus) (D?ptera: Culicidae)
spellingShingle Influ?ncia da densidade populacional de larvas no conte?do de triacilglicerol, fecundidade e tamanho de f?meas em Aedes aegypti (Linnaeus) (D?ptera: Culicidae)
Silva, Elaine Rodrigues Miranda Nery da
Aedes aegypti
triacilglicerol
densidade
mosquitos
triacylglycerol
density
mosquitoes
Bioqu?mica
title_short Influ?ncia da densidade populacional de larvas no conte?do de triacilglicerol, fecundidade e tamanho de f?meas em Aedes aegypti (Linnaeus) (D?ptera: Culicidae)
title_full Influ?ncia da densidade populacional de larvas no conte?do de triacilglicerol, fecundidade e tamanho de f?meas em Aedes aegypti (Linnaeus) (D?ptera: Culicidae)
title_fullStr Influ?ncia da densidade populacional de larvas no conte?do de triacilglicerol, fecundidade e tamanho de f?meas em Aedes aegypti (Linnaeus) (D?ptera: Culicidae)
title_full_unstemmed Influ?ncia da densidade populacional de larvas no conte?do de triacilglicerol, fecundidade e tamanho de f?meas em Aedes aegypti (Linnaeus) (D?ptera: Culicidae)
title_sort Influ?ncia da densidade populacional de larvas no conte?do de triacilglicerol, fecundidade e tamanho de f?meas em Aedes aegypti (Linnaeus) (D?ptera: Culicidae)
author Silva, Elaine Rodrigues Miranda Nery da
author_facet Silva, Elaine Rodrigues Miranda Nery da
author_role author
dc.contributor.advisor1.fl_str_mv Pontes, Emerson Guedes
dc.contributor.advisor1ID.fl_str_mv 045.534.107-96
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/1562085358907265
dc.contributor.referee1.fl_str_mv Pontes, Emerson Guedes
dc.contributor.referee2.fl_str_mv Mallet, Jacenir Reis dos Santos
dc.contributor.referee3.fl_str_mv Oliveira, Marise Maleck de
dc.contributor.referee4.fl_str_mv Silva, Julia dos Santos
dc.contributor.authorID.fl_str_mv 147.655.907-47
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/6683860713209935
dc.contributor.author.fl_str_mv Silva, Elaine Rodrigues Miranda Nery da
contributor_str_mv Pontes, Emerson Guedes
Pontes, Emerson Guedes
Mallet, Jacenir Reis dos Santos
Oliveira, Marise Maleck de
Silva, Julia dos Santos
dc.subject.por.fl_str_mv Aedes aegypti
triacilglicerol
densidade
mosquitos
topic Aedes aegypti
triacilglicerol
densidade
mosquitos
triacylglycerol
density
mosquitoes
Bioqu?mica
dc.subject.eng.fl_str_mv triacylglycerol
density
mosquitoes
dc.subject.cnpq.fl_str_mv Bioqu?mica
description Given the importance of the Aedes aegypti as arboviroses vector, understanding the impact of abiotic factors, such as population density in the larval rearing s, during larval development, is fundamental to improve control methods. The aims of this work were to analyze the effects of population density on the triacylglycerol (TAG) reserves, fecundity and the size of mosquitos. To evaluate the influence of density on the amount of triacylglycerol in the different stages of development of A. aegypti, the larvae were reared in the following densities 0.04, 0.32 and 0.8 larvae / ml, represented by D1, D2 and D3, respectively. It was observed that different larval rearing affected the amount of triacylglycerol on the different development stages of this insect. The group reared in a high population density (D3) presented greater storage of triacylglycerol, except when analyzing the amount of triacylglycerol ?g/protein of fat body in blood-fed females. In addition, it was possible to observe a higher resistance to fasting, higher amount of laid eggs. Insects reared on D2 and D3 were larger than D1. This study provided interesting data for future investigations as to how densitiy of larval rearing affect the lipid metabolism concerning to synthesis and mobilization of triacylglycerol store in A. aegypti and how does it influences it size and the reproduction.
publishDate 2020
dc.date.issued.fl_str_mv 2020-11-30
dc.date.accessioned.fl_str_mv 2022-12-12T20:28:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA, Elaine Rodrigues Miranda Nery da. Influ?ncia da densidade populacional de larvas no conte?do de triacilglicerol, fecundidade e tamanho de f?meas em Aedes aegypti (Linnaeus) (D?ptera: Culicidae). 2020. 41 f. Disserta??o (Mestrado em Biologia Animal) - Instituto de Ci?ncias Biol?gicas e da Sa?de, Universidade Federal Rural do Rio de Janeiro, Serop?dica, RJ. 2020.
dc.identifier.uri.fl_str_mv https://tede.ufrrj.br/jspui/handle/jspui/6129
identifier_str_mv SILVA, Elaine Rodrigues Miranda Nery da. Influ?ncia da densidade populacional de larvas no conte?do de triacilglicerol, fecundidade e tamanho de f?meas em Aedes aegypti (Linnaeus) (D?ptera: Culicidae). 2020. 41 f. Disserta??o (Mestrado em Biologia Animal) - Instituto de Ci?ncias Biol?gicas e da Sa?de, Universidade Federal Rural do Rio de Janeiro, Serop?dica, RJ. 2020.
url https://tede.ufrrj.br/jspui/handle/jspui/6129
dc.language.iso.fl_str_mv por
language por
dc.relation.references.por.fl_str_mv ALTO B. W.; MUT, E. J AND LAMPMAN, R. L. Effects of nutrition and density in Culex pipiens. Medical and Veterinary Entomology, v. 7200, p. 396?406. 2012. ARRESE, E. L.; SOULAGES, J. L. Insect fat body: energy, metabolism, and regulation. Annual Review of Entomology, v. 55, n. 87, p. 207?225. 2010. BALDAL, E. A.; LINDE, K. VAN DER; ALPHEN, J. J. M. VAN. The effects of larval density on adult life-history traits in three species of Drosophila. Mechanisms of Ageing and Development 126 v. 126, p. 407?416. 2005. BALEBA, S. B. S., MASIGA, D., TORTO, B., WELDON, C. W., & GETAHUN, M. N.. Effect of larval density and substrate quality on the wing geometry of Stomoxys calcitrans L. (Diptera: Muscidae). Parasites & Vectors, 12(1), 222. 2019. BARLETTA, A. B. F. et al. Emerging role of lipid droplets in Aedes aegypti immune response against bacteria and Dengue virus. Scientific Reports, v. 6, n. September 2015, p. 1?13. 2016. BESERRA, E. B.; FERNANDES, C. R. M.; RIBEIRO, P. S. Rela??o entre densidade larval e ciclo de vida, tamanho e fecundidade de Aedes (Stegomyia) aegypti (l.) (Diptera: Culicidae) em laborat?rio. Neotropical Entomology, v. 38, n. 6, p. 847?852. 2009. BORASH, D. J.; HO, G. T. Patterns of selection: Stress resistance and energy storage in density-dependent populations of Drosophila melanogaster. Journal of Insect Physiology, v. 47, n. 12, p. 1349?1356. 2001. BRIEGEL, H. Metabolic relationship between female body size, reserves, and fecondity of Aedes aegypti. J.Insect.Physiol., v. 36, n. 3, p. 165?172. 1990. BRIEGEL, H. Physiological bases of mosquito ecology. Journal of Vector Ecology. p. 1?11. 2003. BRIEGEL, H.; HEFTI, M.; DIMARCO, E. Lipid metabolism during sequential gonotrophic cycles in large and small female Aedes aegypti. Journal of Insect Physiology.v. 48, p. 547?554. 2002. BROWN, J. E.; EVANS, B. R.; ZHENG, W.; OBAS, V.; MARTINEZ, L. B.; EGIZI, A.; ZHAO, H.; CACCONE, A.; POWELL, J. R.et al. Human impacts have shaped historical and recent evolution in Aedes aegypti , the dengue and yellow fever mosquito. The Society for the Study of Evolution . p. 514?525. 2013. CHUNG, H.-N. et al. Fat Body Organ Culture System in Aedes Aegypti, a Vector of Zika Virus. Journal of Visualized Experiments, n. 126, p. 1?8. 2017. CLIFTON, M. E.; NORIEGA, F. G. The fate of follicles after a blood meal is dependent on previtellogenic nutrition and juvenile hormone in Aedes aegypti. Journal of Insect Physiology, v. 58, n. 7, p. 1007?1019. 2012. COSTA, F. S. et al. Din?mica populacional de Aedes aegypti (L) em ?rea urbana de alta incid?ncia de dengue. Revista da Sociedade Brasileira de Medicina Tropical, v. 41, n. 3, p. 309?312. 2008. COURET, J.; BENEDICT, M. Q. A meta-analysis of the factors influencing development rate variation in Aedes aegypti ( Diptera : Culicidae ). BMC Ecology, 2014,p. 1?15. 2014. COURET, J.; DOTSON, E.; BENEDICT, M. Q. Temperature, larval diet, and density effects on development rate and survival of Aedes aegypti (Diptera: Culicidae). PLOS ONE, 9(2). 2014. DICKENS, B. L. et al. Determining environmental and anthropogenic factors which explain the global distribution of Aedes aegypti and Ae. albopictus. BMJ Global Health, v. 3, n. 4, p. 2018. FARNESI, L. C. et al. Darker eggs of mosquitoes resist more to dry conditions: Melanin enhances serosal cuticle contribution in egg resistance to desiccation in Aedes, Anopheles and Culex vectors. PLoS Neglected Tropical Diseases, v. 11, n. 10, p. 1? 20. 2017. FARNESI, L. C. et al. The influence of a light and dark cycle on the egg laying activity of Aedes aegypti ( Linnaeus , 1762 ) ( Diptera : Culicidae ). Mem Inst Oswaldo Cruz. v. 113, n. 4, p. 4?9. 2018. FIOCRUZ. Dengue. Instru??es para Pessoal de Combate ao Vetor. Manual de Normas T?nicas. FUNASA. 2001. ATTARDO, G. M.; HANSEN, I. A. S. H. S.; RAIKHEL, A. S Identification of two cationic amino acid transporters required for nutritional signaling during mosquito reproduction. Journal of Experimental Biology, v. 209, n. 16, p. 3071?3078. 2006. GIBBONS, G. F.; ISLAM, K.; PEASE, R. J. Mobilisation of triacylglycerol stores. Biochimica et biophysica acta v. 1483. 2000. GILBERT, L. I.; CHINO, H. Transport of lipids in insects. J. Lipid Res., v. 15, n. 5, p. 439? 456, set. 1974. GILLES, J. R. L. et al. Density-Dependent Effects in Experimental Larval Populations of Anopheles arabiensis (Diptera: Culicidae) Can Be Negative, Neutral, or Overcompensatory Depending on Density and Diet Levels. Journal of Medical Entomology, v. 48, n. 2, p. 296?304. 2011. GONDIM, K; ATELLA, G, PONTES, E; MAJEROWICZ, D. Lipid metabolism in insect disease vectors. Insect Biochemistry and Molecular Biology, 101, 108?123. 2018. HEINISCH E SILVA, M. R. et al. Seasonal and spatial distribution of Aedes aegypti and Aedes albopictus in a municipal urban park in S?o Paulo, SP, Brazil. Acta Tropica. 2018. HURD, H. Manipulation of medically important insect vectors by their parasites. Annual Review of Entomology, v. 48, n. 1, p. 141?161, 2003. IMAM, H.; SOFI, G.; AZIZ, S. The basic rules and methods of mosquito rearing (Aedes aegypti). Tropical Parasitology. v. 4, n. 1, p. 53?55. 2014. JIRAKANJANAKIT, N. et al. Influence of larval density or food variation on the geometry of the wing of Aedes ( Stegomyia ) aegypti. Tropical Medicine and International Health. volume v. 12, n. 11, p. 1354?1360. 2007. JUNJHON, J. et al. Ultrastructural characterization and three-dimensional architecture of replication sites in dengue virus-infected mosquito cells. Journal of Virology. 88, 4687?4697. 2014. KABA, D., BERTE, D., Ta Bi Tra, D., TELLER? ?a, J., SOLANO, P., DUJARDIN, J.-P., The wing venation patterns to identify single tsetse flies. 2016. doi:10.1016/j.meegid.2016.10.008 KANTOR, I. N. Dengue, Zika and Chikungunya. Medicina, p. 1?5. 2016. KRAEMER, M. U. G. et al. The global distribution of the arbovirus vectors Aedes aegypti and Ae . albopictus. p. 1?18. 2015. doi: 10.7554/eLife.08347 LEHMANN, T.. DALTON, R; KIM, E. H.; DAHL, E.; DIABATE, A.; DABIRE, R; DUJARDIN, J. P. Genetic contribution to variation in larval development time , adult size , and longevity of starved adults of Anopheles gambiae. Infection, Genetics and Evolution. v. 6, p. 410?416. 2006. LING, L.; KOKOZA, A. V.; ZHANG, C.; ARKZOY, E.; RAIKHEL, A. S. MicroRNA-277 targets insulin-like peptides 7 and 8 to control lipid metabolism and reproduction in Aedes aegypti mosquitoes. Proceedings of the National Academy of Sciences, v. 114, n. 38, p. E8017?E8024. 2017. LONG, K. C. et al. Experimental Transmission of Mayaro Virus by Aedes aegypti. v. 85, n. 4, p. 750?757. 2011. doi: https://doi.org/10.4269/ajtmh.2011.11-0359 LORENZ, C.; ALMEIDA, F.; LOPES, F. A.; LOUISE, C; PEREIRA, S. N.; PETERSEN, V.; VIDAL, P. O.; SUESDEK , F. V. L.; Geometric morphometrics in mosquitoes: What has been measured?. Infection, Genetics and Evolution. 2017. doi: 10.1016/j.meegid.2017.06.029 LOUNIBOS, L P;NISHIMURA, N; CONN, J;OLIVEIRA, R. Life history correlates of adult size in the Malaria Vector Anopheles darlingi. M?m?rias Instituto Oswaldo Cruz, vol 90 (6): 769 - 774. 1995. MARTINS, R. et al. Hypometabolic strategy and glucose metabolism maintenance of Aedes aegypti egg desiccation. Comparative Biochemistry and Physiology, Part B, p. 2018. MENDES, A. M. et al. Conserved mosquito/parasite interactions affect development of Plasmodium falciparum in Africa. PLoS Pathogens, v. 4, n. 5. 2008. MOORE, C. G AND FISHER, B. R. Competition in Mosquitoes. Density and Secies Ratio Effects on Gronwth, Mortaity, Fecundity, and Production of Growth Retardant. Annals of the entomological society of America, v. 62, n. 6. 1969. MOSTOWY, W. M.; FOSTER, W. A. Antagonistic effects of energy status on meal size and egg-batch size of Aedes aegypti ( Diptera : Culicidae ). Journal of Vector Ecology. p. 84?93. 2004. NATTERO, J.; PICCINALI, R. V.; LOPES, C. M.; HERN?NDEZ, M. L.; ABRAHAN, L.; LOBBIA, P. A.; RODR?GUEZ, C. S.; FUENTE, A. L. C. Morphometric variability among the species of the Sordida subcomplex ( Hemiptera : Reduviidae : Triatominae ): evidence for differentiation across the distribution range of Triatoma sordida. Parasites & Vectors p. 1?14. 2017. PERERA R, RILEY C, ISAAC G, HOPF-JANNASCH AS, MOORE RJ, et al. Dengue Virus Infection Perturbs Lipid Homeostasis in Infected Mosquito Cells. PLoS Pathogens 8(3): e1002584. 2012. doi:10.1371/journal.ppat.1002584 PETERSON, G. L. A Simplification of the Protein Assay Method of Lowry et al . Analytical Biochemistry. Which is More Generally Applicable. v. 356, p. 346?356. 1977. PRICE, D. P. et al. Small mosquitoes, large implications: crowding and starvation affects gene expression and nutrient accumulation in Aedes aegypti. Parasites & vectors, v. 8, n. 1, p. 252. 2015. QUARESMA, M. A. Avalia??o da implanta??o do componente controle vetorial do Programa de Controle da Dengue em Porto Seguro-BA/Brasil. [s.l.] FIOCRUZ. 2017. REZENDE, G. L. et al. Embryonic desiccation resistance in Aedes aegypti: Presumptive role of the chitinized Serosal Cuticle. BMC Developmental Biology, v. 8, p. 1?14. 2008. ROWLAND, M.; BOERSMA, E. Changes in the spontaneous flight activity of the mosquito Anopheles stephensi by parasitization with the rodent malaria Plasmodium yoelii. Parasitology, v. 97, n. 2, p. 221?227. 1988. ROY, S. et al. Regulation of Reproductive Processes in Female Mosquitoes. In: Advances in Insect Physiology. [s.l: s.n.]. v. 51p. 115?144. 2016 SANTOS, C.; LEITE, G.; FALQUETO, A. Does native bromeliads represent important breeding sites for Aedes aegypti (L.) (Diptera: Culicidae) in urbanized areas? Neotropical Entomology, v. 40, n. 2, p. 278?281, 2011. SOULAGES, J. L.; ARRESE. E. L. Insect fat body: energy, metabolism, and regulation. Annu Rev Entomol. 55: 207?225. 2011. doi:10.1146/annurev-ento-112408-085356. TELANG, A. AND WELLS, M. A. The effect of larval and adult nutrition on successful autogenous egg production by a mosquito. Journal of Insect Physiology. v. 50, p. 677? 685. 2004. TELANG, A.; FRAME, L.; BROWN, M. R. Larval feeding duration affects ecdysteroid levels and nutritional reserves regulating pupal commitment in the yellow fever mosquito Aedes aegypti ( Diptera : Culicidae ). The Journal of Experimental Biology. p. 854? 864. 2007. WANG, X. et al. Hormone and receptor interplay in the regulation of mosquito lipid metabolism. PNAS. e2709?e2718. 2017. WEAVER, S. C. COSTA, F., et al. Zika Virus: History, Emergence, Biology, and Prospects for Control. Antiviral research. p. 69?80. 2017. WILKE, A. B. B. et al. Ornamental bromeliads of Miami-Dade County, Florida are important breeding sites for Aedes aegypti (Diptera: Culicidae). Parasites & Vectors, v. 11, n. 1, p. 283. 2018. YOSHIOKA, M. et al. Diet and density dependent competition affect larval performance and oviposition site selection in the mosquito species Aedes albopictus (Diptera : Culicidae). Parasites & Vectors. p. 1?11. 2012. ZARA, L. S. A. SANTOS, M.; OLIVEIRA, S. F. Estrat?gias de controle do Aedes aegypti: uma revis?o. Epidemiologia e Servi?os de Sa?de, v. 25, n. 2, p. 1?2, 2016. ZHOU, G.; PENNINGTON, J. E.; ?, M. A. W. Utilization of pre-existing energy stores of female Aedes aegypti mosquitoes during the first gonotrophic cycle. Insect Biochemistry and Molecular Biology, v. 34, p. 919?925, 2004. ZIEGLER, R.; ANTWERPEN, R. VAN. Lipid uptake by insect oocytes. Insect Biochemistry and Molecular Biology. 36 (2006) 264?272. 2006. SECRETARIA DE ESTADO DE SA?DE DO RIO DE JANEIRO. Boletim epidemiol?gico arboviroses. N? 004. n. 85, p. 1?17. 2018. ZIRBEL, K.; EASTMOND, B.; ALTO, B. W. Parental and offspring larval diets interact to influence life-history traits and infection with dengue virus in Aedes aegypti. R. Soc. open sci. 5: 180539. 2018.
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal Rural do Rio de Janeiro
dc.publisher.program.fl_str_mv Programa de P?s-Gradua??o em Biologia Animal
dc.publisher.initials.fl_str_mv UFRRJ
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Instituto de Ci?ncias Biol?gicas e da Sa?de
publisher.none.fl_str_mv Universidade Federal Rural do Rio de Janeiro
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ
instname:Universidade Federal Rural do Rio de Janeiro (UFRRJ)
instacron:UFRRJ
instname_str Universidade Federal Rural do Rio de Janeiro (UFRRJ)
instacron_str UFRRJ
institution UFRRJ
reponame_str Biblioteca Digital de Teses e Dissertações da UFRRJ
collection Biblioteca Digital de Teses e Dissertações da UFRRJ
bitstream.url.fl_str_mv http://localhost:8080/tede/bitstream/jspui/6129/4/2020+-+Elaine+Rodrigues+Miranda+Nery+da+Silva.pdf.jpg
http://localhost:8080/tede/bitstream/jspui/6129/3/2020+-+Elaine+Rodrigues+Miranda+Nery+da+Silva.pdf.txt
http://localhost:8080/tede/bitstream/jspui/6129/2/2020+-+Elaine+Rodrigues+Miranda+Nery+da+Silva.pdf
http://localhost:8080/tede/bitstream/jspui/6129/1/license.txt
bitstream.checksum.fl_str_mv cc73c4c239a4c332d642ba1e7c7a9fb2
9be62f7a51255272ab86ee36e92fceb5
920ba00caa550ba2451864808c3f175c
7b5ba3d2445355f386edab96125d42b7
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da UFRRJ - Universidade Federal Rural do Rio de Janeiro (UFRRJ)
repository.mail.fl_str_mv bibliot@ufrrj.br||bibliot@ufrrj.br
_version_ 1800313560420581376

Registros relacionados