Caracteriza??o e sele??o de isolados de Beauveria bassiana para o controle microbiano do carrapato Boophilus microplus
Autor(a) principal: | |
---|---|
Data de Publicação: | 2007 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFRRJ |
Texto Completo: | https://tede.ufrrj.br/jspui/handle/tede/805 |
Resumo: | Beauveria bassiana is an entomopathogenic fungus used worldwide as a biological control agent of pest arthropods. The present study aimed at characterizing several isolates of B. bassiana and selects those with major potential to control ticks population and other arthropods under natural conditions. Thus, 53 isolates of B. bassiana and 7 isolates including 5 other Beauveria spp. and 1 Engyodontium albus (=Beauveria alba) were investigated. These isolates were originally from different regions of Brazil and other nations, and different hosts or substrate. Initially, all the isolates were morphologically analyzed and investigated about the potential of conidial production. Afterwards, experiments were conducted to investigate the genetic diversity of Brazilian isolates of B. bassiana, to evaluate the spatial distribution of genotypes, and to understand the genetic relationship among isolates of different species of Beauveria. Thus, all isolates were analyzed by isozymes electrophoresis, AFLP-PCR and sequencing of ITS1 and ITS2 regions. The results have permitted the taxonomic identification of isolates and the selection of those with major potential to conidial production. The results have also showed the detection of high genetic variability among B. bassiana isolates, indicating that this fungus represents a species aggregate. Furthermore, the genetic distance among isolates was directly related to the geographic distances. In addition, the isolates were evaluated to UV-B tolerance, heat tolerance, and cold activity. Heat and cold are two abiotic factors of the environment that restrict the use of entomopathogenic fungi as agents for biological control of insects. In addition, solar radiation, particularly the UV-B wavelength, is a major environmental factor that can negatively affect this entomopathogenic fungus in the field. High variability in UV-B tolerance was detected among the isolates after 2 h of radiation exposure. Conidial relative germination ranged from 0% to almost 80%, and a delayed germination was detected. Moreover, isolates of B. bassiana originating from lower latitudes tended to have lower UV-B tolerance than isolates from higher latitudes. A high variability in thermotolerance was also observed among the isolates after 2 h of heat exposure at 45 ?C, ranging from low, to medium, to high viability. The thermal death point of most resistant isolates was between 44 ?C and 45 ?C after 6 h of heat exposure. At cold temperatures most of the isolates grew at 5 ?C. An attempt to correlate the latitude of origin with thermotolerance or cold activity indicated that isolates from higher latitudes were more cold-active than isolates from nearer the equator. The present study also evaluated the virulence of the isolates toward Boophilus microplus larvae, and virulence ranged from very low to very high. It was observed that different populations of B. microplus may present different susceptibility to B. bassiana infection. Therefore, the present study has selected isolates of this entomopathogenic fungus with major effective potential to control B. microplus and other pest arthropods under environmental conditions. |
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Bittencourt, V?nia Rita Elias Pinheiro505.198.676-53http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786415J1Costa, Gisela Lara da859.362.867-20http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785611J5Pacheco, Raquel da Silva611.339.147-72http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788615Y3071.248.587-20http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4707291J3Fernandes, ?verton Kort Kamp2016-04-28T20:16:21Z2007-05-242007-03-30FERNANDES, ?verton Kort Kamp. Caracteriza??o e sele??o de isolados de Beauveria bassiana para o controle microbiano do carrapato Boophilus microplus. 2007. 148 f. Tese (Doutorado em Ci?ncias Veterin?rias) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2007.https://tede.ufrrj.br/jspui/handle/tede/805Beauveria bassiana is an entomopathogenic fungus used worldwide as a biological control agent of pest arthropods. The present study aimed at characterizing several isolates of B. bassiana and selects those with major potential to control ticks population and other arthropods under natural conditions. Thus, 53 isolates of B. bassiana and 7 isolates including 5 other Beauveria spp. and 1 Engyodontium albus (=Beauveria alba) were investigated. These isolates were originally from different regions of Brazil and other nations, and different hosts or substrate. Initially, all the isolates were morphologically analyzed and investigated about the potential of conidial production. Afterwards, experiments were conducted to investigate the genetic diversity of Brazilian isolates of B. bassiana, to evaluate the spatial distribution of genotypes, and to understand the genetic relationship among isolates of different species of Beauveria. Thus, all isolates were analyzed by isozymes electrophoresis, AFLP-PCR and sequencing of ITS1 and ITS2 regions. The results have permitted the taxonomic identification of isolates and the selection of those with major potential to conidial production. The results have also showed the detection of high genetic variability among B. bassiana isolates, indicating that this fungus represents a species aggregate. Furthermore, the genetic distance among isolates was directly related to the geographic distances. In addition, the isolates were evaluated to UV-B tolerance, heat tolerance, and cold activity. Heat and cold are two abiotic factors of the environment that restrict the use of entomopathogenic fungi as agents for biological control of insects. In addition, solar radiation, particularly the UV-B wavelength, is a major environmental factor that can negatively affect this entomopathogenic fungus in the field. High variability in UV-B tolerance was detected among the isolates after 2 h of radiation exposure. Conidial relative germination ranged from 0% to almost 80%, and a delayed germination was detected. Moreover, isolates of B. bassiana originating from lower latitudes tended to have lower UV-B tolerance than isolates from higher latitudes. A high variability in thermotolerance was also observed among the isolates after 2 h of heat exposure at 45 ?C, ranging from low, to medium, to high viability. The thermal death point of most resistant isolates was between 44 ?C and 45 ?C after 6 h of heat exposure. At cold temperatures most of the isolates grew at 5 ?C. An attempt to correlate the latitude of origin with thermotolerance or cold activity indicated that isolates from higher latitudes were more cold-active than isolates from nearer the equator. The present study also evaluated the virulence of the isolates toward Boophilus microplus larvae, and virulence ranged from very low to very high. It was observed that different populations of B. microplus may present different susceptibility to B. bassiana infection. Therefore, the present study has selected isolates of this entomopathogenic fungus with major effective potential to control B. microplus and other pest arthropods under environmental conditions.Beauveria bassiana ? um fungo entomopatog?nico utilizado em v?rias partes do mundo como agente de controle biol?gico de artr?podes. Baseado nisso, o presente estudo buscou caracterizar diversos isolados de B. bassiana e selecionar aqueles com maior potencial para controlar popula??es de carrapatos e outros artr?podes em condi??es naturais. Neste sentido, 53 isolados de B. bassiana e mais sete isolados incluindo cinco esp?cies de Beauveria spp. e uma de Engyodontium albus (=Beauveria alba) foram estudadas. Esses isolados foram oriundos de v?rias regi?es geogr?ficas brasileiras e estrangeiras, e de diferentes hospedeiros ou substratos. Os isolados foram inicialmente avaliados morfologicamente e quanto ao potencial de produ??o de con?dios. Em seguida, buscou-se investigar a diversidade gen?tica dos isolados brasileiros de B. bassiana, avaliar a distribui??o espacial de gen?tipos, e conhecer a rela??o gen?tica entre os isolados das diferentes esp?cies. Para esta finalidade os isolados foram submetidos as t?cnicas de eletroforese de isoenzimas, AFLP-PCR e seq?enciamento de regi?es ITS1 e ITS2. Os resultados permitiram a confirma??o taxon?mica dos isolados e a sele??o daqueles com maior capacidade de produ??o de con?dios. Permitiram tamb?m detectar grande variabilidade gen?tica entre os isolados de B. bassiana indicando que este fungo representa um agregado de esp?cies. Al?m disso, a dist?ncia gen?tica detectada entre os isolados foi fortemente relacionada a dist?ncia geogr?fica de origem dos isolados. Posteriormente, os isolados tamb?m foram avaliados quanto a toler?ncia ? radia??o UV-B, toler?ncia ao calor e atividade ao frio. Tanto o calor quanto o frio s?o fatores abi?ticos ambientais que restringem o uso de fungos como agentes de biocontrole, enquanto a radia??o solar, particularmente UV-B, ? o maior fator ambiental que pode afetar negativamente a a??o deste entomopat?geno. Neste sentido, foi observada alta variabilidade entre os isolados analisados em rela??o ? toler?ncia a UV-B ap?s duas horas de exposi??o ? radia??o, obtendose percentuais de germina??o de con?dios que variaram entre 0% e aproximadamente 80%. Al?m disso, foi observado atraso na germina??o dos con?dios, e revelado que os isolados originados de baixas latitudes tenderam a apresentar menor toler?nc ia a UV-B do que aqueles provenientes de latitudes mais elevadas. Foi tamb?m encontrada alta variabilidade em termotoler?ncia dos con?dios ap?s duas horas de exposi??o a 45 ?C, variando entre baixa, m?dia e alta viabilidade. Os con?dios mais resistentes n?o toleraram exposi??o entre 44 ?C e 45 ?C por seis horas. Quando submetidos a 5 ?C a maioria dos isolados apresentaram atividade. Os isolados de B. bassiana obtidos de latitudes mais elevadas foram mais ativos ao frio do que aqueles originados pr?ximos ? linha do equador. Al?m disso, os isolados tamb?m foram avaliados quanto ao potencial virulento sobre larvas de Boophilus microplus, e neste sentido foi poss?vel diferenci?-los em baixa, m?dia e alta virul?ncia. Foi detectado tamb?m que diferentes popula??es desta esp?cie de carrapato podem manifestar diferentes n?veis de suscetibilidade ? infec??o por B. bassiana. Neste sentido, o presente estudo permitiu selecionar isolados deste entomopat?geno com maior potencial para o controle biol?gico de B. microplus e outros artr?podes em condi??es de campo.Made available in DSpace on 2016-04-28T20:16:21Z (GMT). No. of bitstreams: 1 2007 - ?verton Kort Kamp Fernandes.pdf: 2844359 bytes, checksum: a56e9f806dc8f1f0e7cc6b4641b4a78c (MD5) Previous issue date: 2007-03-30Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPqapplication/pdfhttps://tede.ufrrj.br/retrieve/59884/2007%20-%20%c3%89verton%20Kort%20Kamp%20Fernandes.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Ci?ncias Veterin?riasUFRRJBrasilInstituto de Veterin?riacontrole biol?gicoBoophilus microplusBeauveria sppbiological controlMedicina Veterin?riaCaracteriza??o e sele??o de isolados de Beauveria bassiana para o controle microbiano do carrapato Boophilus microplusCharacterization and selection of Beauveria bassiana isolates to microbiological control of Boophilus microplus tickinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRRJinstname:Universidade Federal Rural do Rio de Janeiro (UFRRJ)instacron:UFRRJTHUMBNAIL2007 - ?verton Kort Kamp Fernandes.pdf.jpg2007 - ?verton Kort Kamp Fernandes.pdf.jpgimage/jpeg1943http://localhost:8080/tede/bitstream/tede/805/3/2007+-+%C3%89verton+Kort+Kamp+Fernandes.pdf.jpg8c1280dca4077478b5fad29883580c72MD53TEXT2007 - ?verton Kort Kamp Fernandes.pdf.txt2007 - ?verton Kort Kamp Fernandes.pdf.txttext/plain344343http://localhost:8080/tede/bitstream/tede/805/2/2007+-+%C3%89verton+Kort+Kamp+Fernandes.pdf.txt65a72c6f4a8df5b732245dcc9ecec947MD52ORIGINAL2007 - ?verton Kort Kamp Fernandes.pdf2007 - ?verton Kort Kamp Fernandes.pdfapplication/pdf2844482http://localhost:8080/tede/bitstream/tede/805/1/2007+-+%C3%89verton+Kort+Kamp+Fernandes.pdf959f61f774506ebbe407d8651420aa03MD51tede/8052022-06-13 11:39:39.539oai:localhost:tede/805Biblioteca Digital de Teses e Dissertaçõeshttps://tede.ufrrj.br/PUBhttps://tede.ufrrj.br/oai/requestbibliot@ufrrj.br||bibliot@ufrrj.bropendoar:2022-06-13T14:39:39Biblioteca Digital de Teses e Dissertações da UFRRJ - Universidade Federal Rural do Rio de Janeiro (UFRRJ)false |
dc.title.por.fl_str_mv |
Caracteriza??o e sele??o de isolados de Beauveria bassiana para o controle microbiano do carrapato Boophilus microplus |
dc.title.alternative.eng.fl_str_mv |
Characterization and selection of Beauveria bassiana isolates to microbiological control of Boophilus microplus tick |
title |
Caracteriza??o e sele??o de isolados de Beauveria bassiana para o controle microbiano do carrapato Boophilus microplus |
spellingShingle |
Caracteriza??o e sele??o de isolados de Beauveria bassiana para o controle microbiano do carrapato Boophilus microplus Fernandes, ?verton Kort Kamp controle biol?gico Boophilus microplus Beauveria spp biological control Medicina Veterin?ria |
title_short |
Caracteriza??o e sele??o de isolados de Beauveria bassiana para o controle microbiano do carrapato Boophilus microplus |
title_full |
Caracteriza??o e sele??o de isolados de Beauveria bassiana para o controle microbiano do carrapato Boophilus microplus |
title_fullStr |
Caracteriza??o e sele??o de isolados de Beauveria bassiana para o controle microbiano do carrapato Boophilus microplus |
title_full_unstemmed |
Caracteriza??o e sele??o de isolados de Beauveria bassiana para o controle microbiano do carrapato Boophilus microplus |
title_sort |
Caracteriza??o e sele??o de isolados de Beauveria bassiana para o controle microbiano do carrapato Boophilus microplus |
author |
Fernandes, ?verton Kort Kamp |
author_facet |
Fernandes, ?verton Kort Kamp |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Bittencourt, V?nia Rita Elias Pinheiro |
dc.contributor.advisor1ID.fl_str_mv |
505.198.676-53 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786415J1 |
dc.contributor.advisor-co1.fl_str_mv |
Costa, Gisela Lara da |
dc.contributor.advisor-co1ID.fl_str_mv |
859.362.867-20 |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785611J5 |
dc.contributor.advisor-co2.fl_str_mv |
Pacheco, Raquel da Silva |
dc.contributor.advisor-co2ID.fl_str_mv |
611.339.147-72 |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788615Y3 |
dc.contributor.authorID.fl_str_mv |
071.248.587-20 |
dc.contributor.authorLattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4707291J3 |
dc.contributor.author.fl_str_mv |
Fernandes, ?verton Kort Kamp |
contributor_str_mv |
Bittencourt, V?nia Rita Elias Pinheiro Costa, Gisela Lara da Pacheco, Raquel da Silva |
dc.subject.por.fl_str_mv |
controle biol?gico Boophilus microplus Beauveria spp |
topic |
controle biol?gico Boophilus microplus Beauveria spp biological control Medicina Veterin?ria |
dc.subject.eng.fl_str_mv |
biological control |
dc.subject.cnpq.fl_str_mv |
Medicina Veterin?ria |
description |
Beauveria bassiana is an entomopathogenic fungus used worldwide as a biological control agent of pest arthropods. The present study aimed at characterizing several isolates of B. bassiana and selects those with major potential to control ticks population and other arthropods under natural conditions. Thus, 53 isolates of B. bassiana and 7 isolates including 5 other Beauveria spp. and 1 Engyodontium albus (=Beauveria alba) were investigated. These isolates were originally from different regions of Brazil and other nations, and different hosts or substrate. Initially, all the isolates were morphologically analyzed and investigated about the potential of conidial production. Afterwards, experiments were conducted to investigate the genetic diversity of Brazilian isolates of B. bassiana, to evaluate the spatial distribution of genotypes, and to understand the genetic relationship among isolates of different species of Beauveria. Thus, all isolates were analyzed by isozymes electrophoresis, AFLP-PCR and sequencing of ITS1 and ITS2 regions. The results have permitted the taxonomic identification of isolates and the selection of those with major potential to conidial production. The results have also showed the detection of high genetic variability among B. bassiana isolates, indicating that this fungus represents a species aggregate. Furthermore, the genetic distance among isolates was directly related to the geographic distances. In addition, the isolates were evaluated to UV-B tolerance, heat tolerance, and cold activity. Heat and cold are two abiotic factors of the environment that restrict the use of entomopathogenic fungi as agents for biological control of insects. In addition, solar radiation, particularly the UV-B wavelength, is a major environmental factor that can negatively affect this entomopathogenic fungus in the field. High variability in UV-B tolerance was detected among the isolates after 2 h of radiation exposure. Conidial relative germination ranged from 0% to almost 80%, and a delayed germination was detected. Moreover, isolates of B. bassiana originating from lower latitudes tended to have lower UV-B tolerance than isolates from higher latitudes. A high variability in thermotolerance was also observed among the isolates after 2 h of heat exposure at 45 ?C, ranging from low, to medium, to high viability. The thermal death point of most resistant isolates was between 44 ?C and 45 ?C after 6 h of heat exposure. At cold temperatures most of the isolates grew at 5 ?C. An attempt to correlate the latitude of origin with thermotolerance or cold activity indicated that isolates from higher latitudes were more cold-active than isolates from nearer the equator. The present study also evaluated the virulence of the isolates toward Boophilus microplus larvae, and virulence ranged from very low to very high. It was observed that different populations of B. microplus may present different susceptibility to B. bassiana infection. Therefore, the present study has selected isolates of this entomopathogenic fungus with major effective potential to control B. microplus and other pest arthropods under environmental conditions. |
publishDate |
2007 |
dc.date.available.fl_str_mv |
2007-05-24 |
dc.date.issued.fl_str_mv |
2007-03-30 |
dc.date.accessioned.fl_str_mv |
2016-04-28T20:16:21Z |
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FERNANDES, ?verton Kort Kamp. Caracteriza??o e sele??o de isolados de Beauveria bassiana para o controle microbiano do carrapato Boophilus microplus. 2007. 148 f. Tese (Doutorado em Ci?ncias Veterin?rias) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2007. |
dc.identifier.uri.fl_str_mv |
https://tede.ufrrj.br/jspui/handle/tede/805 |
identifier_str_mv |
FERNANDES, ?verton Kort Kamp. Caracteriza??o e sele??o de isolados de Beauveria bassiana para o controle microbiano do carrapato Boophilus microplus. 2007. 148 f. Tese (Doutorado em Ci?ncias Veterin?rias) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2007. |
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https://tede.ufrrj.br/jspui/handle/tede/805 |
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Universidade Federal Rural do Rio de Janeiro |
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Programa de P?s-Gradua??o em Ci?ncias Veterin?rias |
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UFRRJ |
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Brasil |
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Instituto de Veterin?ria |
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Universidade Federal Rural do Rio de Janeiro |
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