A??o de diferentes desinfetantes na viabilidade e desenvolvimento de ovos e na migra??o larvar de Toxocara cati (Schrank,1788) em camundongos
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Data de Publicação: | 2011 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFRRJ |
Texto Completo: | https://tede.ufrrj.br/jspui/handle/jspui/3810 |
Resumo: | The Toxocara cati is one of the major intestinal parasites of cats and has great importance in public health as zoonotic agent of Visceral and Ocular Larva Migrans in humans. Studies to eliminate the infectious forms of this helminths are scarce. The aim of this study was to evaluate the action of various disinfectant solutions on the development of eggs of T. cati viability and infectivity of larvae present in the eggs of this parasite. Eggs were obtained from the dissection of female T. cati, recovered from infected cats. These were suspended in distilled water and divided into five groups with six replicates, each with about 3300 eggs. Each group was exposed for one hour at one of four selected commercial disinfectants and a control, and then washed with distilled water for removal of disinfectant solutions. All samples were assessed for development of eggs on days 0, +3, +6, +9, +12, +15, +18, +21 and +24. At the end of the observation period, 30 mice were infected with the samples. After 15 days the mice were euthanized and the brain, liver, lungs and kidneys, and carcass, macro-and microscopically observed. From day +6 eggs exposed to different treatments showed larva inside. And even after 24 days of exposure, the disinfectants were not able to inhibit the development or eliminate the larvae. The number of larvae recovered from infected mice showed that migration was observed in all animals. The carcass showed better recovery between the tissues analyzed. In no animal treated larvae were seen in the brain. No disinfectant used was able to inhibit embryogenesis of eggs of T. cati after one hour of exposure. The degree of infectivity and the migration pattern of the larvae was maintained even after treatment with disinfectants |
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Scott, Fabio Barbour00138216797http://lattes.cnpq.br/1217253540572819Scott, Fabio BarbourTancredi, Ian PhilippoMartins, Isabella Vilhena Freire09801343745xxxxxxxxxxxxxxxxxTavares, Pedro Vianna2020-08-13T14:11:27Z2011-03-30TAVARES, Pedro Vianna. A??o de diferentes desinfetantes na viabilidade e desenvolvimento de ovos e na migra??o larvar de Toxocara cati (SCHRANK, 1788) em camundongos. 2011. 38 f. Disserta??o (Mestrado em Ci?ncias Veterin?rias) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2011.https://tede.ufrrj.br/jspui/handle/jspui/3810The Toxocara cati is one of the major intestinal parasites of cats and has great importance in public health as zoonotic agent of Visceral and Ocular Larva Migrans in humans. Studies to eliminate the infectious forms of this helminths are scarce. The aim of this study was to evaluate the action of various disinfectant solutions on the development of eggs of T. cati viability and infectivity of larvae present in the eggs of this parasite. Eggs were obtained from the dissection of female T. cati, recovered from infected cats. These were suspended in distilled water and divided into five groups with six replicates, each with about 3300 eggs. Each group was exposed for one hour at one of four selected commercial disinfectants and a control, and then washed with distilled water for removal of disinfectant solutions. All samples were assessed for development of eggs on days 0, +3, +6, +9, +12, +15, +18, +21 and +24. At the end of the observation period, 30 mice were infected with the samples. After 15 days the mice were euthanized and the brain, liver, lungs and kidneys, and carcass, macro-and microscopically observed. From day +6 eggs exposed to different treatments showed larva inside. And even after 24 days of exposure, the disinfectants were not able to inhibit the development or eliminate the larvae. The number of larvae recovered from infected mice showed that migration was observed in all animals. The carcass showed better recovery between the tissues analyzed. In no animal treated larvae were seen in the brain. No disinfectant used was able to inhibit embryogenesis of eggs of T. cati after one hour of exposure. The degree of infectivity and the migration pattern of the larvae was maintained even after treatment with disinfectantsO Toxocara cati ? um dos principais parasitos intestinais de gatos e exerce grande import?ncia em sa?de p?blica sendo agente zoon?tico da Larva Migrans Visceral e Ocular em humanos. Estudos para eliminar as formas infectantes deste helminto s?o escassos. O objetivo do presente estudo foi avaliar a a??o de diferentes solu??es desinfetantes sobre o desenvolvimento de ovos de T. cati e a viabilidade e infectividade de larvas presentes em ovos deste parasito. Os ovos foram obtidos a partir da disseca??o de f?meas de T. cati, recuperadas de gatos comprovadamente infectados. Estes foram suspensos em ?gua destilada e divididos em cinco grupos com seis repeti??es, cada qual com aproximadamente 3300 ovos. Cada grupo foi exposto por uma hora, a um dos quatro desinfetantes comerciais selecionados e um controle, e em seguida lavados com ?gua destilada para elimina??o das solu??es desinfetantes. Todas as amostras foram avaliadas quanto ao desenvolvimento dos ovos, atrav?s de al?quotas de 25?L observadas em microsc?pio ?ptico, nos dias 0, +3, +6, +9, +12, +15, +18, +21 e +24. Ao final do per?odo de observa??o, 30 camundongos foram infectados com as amostras obtidas. Ap?s 15 dias os camundongos foram eutanasiados e o c?rebro, f?gado, pulm?es e rins, al?m da carca?a, observados macro e microscopicamente. A partir do dia +6 os ovos expostos aos diferentes tratamentos apresentaram larva no seu interior. E mesmo na avalia??o do dia +24, os desinfetantes n?o foram capazes de inibir o desenvolvimento ou eliminar as larvas. O n?mero das larvas recuperadas dos camundongos infectados demonstrou que houve migra??o larvar em todos os animais. O tecido que apresentou melhor recupera??o entre os grupos foi a carca?a. Em nenhum animal tratado foram visualizadas larvas no c?rebro. Nenhum desinfetante utilizado foi capaz de inibir a embriog?nese dos ovos de T. cati ap?s uma hora de exposi??o aos produtos. O potencial de infectividade e o padr?o de migra??o das larvas foi mantido mesmo ap?s o tratamento com os desinfetantes.Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2020-08-13T14:11:27Z No. of bitstreams: 1 2011 - Pedro Vianna Tavares.pdf: 6588777 bytes, checksum: c75bd1ce0f1b025bc63a9bf81e790517 (MD5)Made available in DSpace on 2020-08-13T14:11:27Z (GMT). No. of bitstreams: 1 2011 - Pedro Vianna Tavares.pdf: 6588777 bytes, checksum: c75bd1ce0f1b025bc63a9bf81e790517 (MD5) Previous issue date: 2011-03-30Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPqapplication/pdfhttps://tede.ufrrj.br/retrieve/61578/2011%20-%20Pedro%20Vianna%20Tavares.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Ci?ncias Veterin?riasUFRRJBrasilInstituto de Veterin?riaABOUGRAIN, A. K.; NAHAISI, M. H.; MADI, N. S.; SAIED, M. M.; GHENGHESH, K. S. Parasitological contamination in salad vegetables in Tripoli-Libya. Food Control, v.21, p.760-762, 2010. ABO-SHEHADA, M. N.; HERBERT, I. V. The migration of larval Toxocara canis in mice II. Veterinary Parasitology, v.17, n.1, p.75-83, 1985. ACHA, P.; SZYFRES, B. Zoonosis y enfermedades transmissibles al hombre y a los animales, Organizaci?n Mundial de la Salud: Washington, 1986. AKAO, N.; TOMODA, M.; HAYASHI, E.; SIZUKI, R.; SHIMIZU-SUGANUMA, M.; SHICHINOHE, K.; FUJITA, K. Cerebellar ataxia due to Toxocara infection in Mongolian gerbils, Meriones unguiculatus. Veterinary Parasitology, v.113, p.229-237, 2003. ALBA-HURTADO, F.; MU?OZ-GUZM?N, M. A.; VALDIVIA-ANDA, G.; T?RTORA, J.L.; ORTEGA-PIERRES, M.G. Toxocara canis: Larval migration dynamics, detection of antibody reactivity to larval excretory?secretory antigens and clinical findings during experimental infection of gerbils (Meriones unguiculatus). Experimental Parasitology, v.122, n.1, p.1-5, 2009. AY?I?EK, H.; YARSAN, E.; SARIMEHMETOGLU, H. O.; TANY?KSEL, M.; GIRGINKARDESLER, N.; ?ZYURT, M. Efficacy of Some Disinfectants on Embryonated Eggs of Toxocara canis. Turkish Journal of Medical Science, v.31, n.1, p.35-39, 2001. AYRES M, AYRES J?NIOR M, AYRES DL, SANTOS AS. Bioestat 5.0: aplica??es estat?sticas nas ?reas das ci?ncias biol?gicas e m?dicas. Bel?m: Sociedade Civil Mamirau? 364p, 2007. 34 BARRIGA, O. O. A Critical Look at the Importance, Prevalence and Control of Toxocariasis and the Possibilities of Immunological Control. Veterinary Parasitology, v.29, n.2-3, p.195- 234, 1988. BEAVER, P. C.; SNYDER, C. H.; CARRERA, G. M.; DENT, J. H., LAFFERTY, J. W. Chronic eosinophilia due to visceral larva migrans: Report of three cases. Pediatrics, v.9, n.1, p.7-19, 1952. BOUCHET, F.; BOULLARD, Y.; BACCAM, D.; LEGER, N. Ultrastructural studies of alterations induced by microwaves in Toxocara canis eggs: prophylactic interest. Z. Parazitenkd, v.72, p.755-765, 1986. BOWMAN, D. D. Parasitologia Veterin?ria de Georgis. 8 ed. Barueri: Manole, 422p. 2006. CARDILLO, N.; ROSA, A.; RIBICICH, M.; L?PEZ, C.; SOMMERFELT, C. Experimental infection with Toxocara cati in BALB/c Mice, migratory behaviour and pathological changes. Zoonoses and Public Health, v.56, n.4, p.198-205, 2009. C?RTES V. A.; PAIM, G. V.; FILHO, R. A. A. Infesta??o por ancilostomideos e toxocarideos em c?es e gatos apreendidos em vias publicas, S?o Paulo. Revista Sa?de Publica, v. 22, n. 4, p. 341-3, 1988. DESPOMMIER, D. Toxocariasis: Clinical Aspects, Epidemiology, Medical Ecology, and Molecular Aspects. Clinical Microbiology Reviews, v.16, n.2, p.265-272, 2003. DUBEY, J. P. Migration of Toxocara cati larvae in mice. Tropical Geographic Medicine, v. 20, n. 2, p. 172-176, 1968. DUBINSKY, P.; HAVASIOVAREITEROVA, K.; PETKO, B.; HOVORKA, I.; TOMASOVICOVA, O. Role of small mammals in the epidemiology of toxocariasis. Parasitology, v.110, n.2, p.187?193, 1995. 35 DUNSMORE, J. D.; THOMPSON, R. C. A.; BATES, I. A. The accumulation of Toxocara canis larvae in the brains of mice. International Journal for Parasitology, v.13, n.5, p.517- 521, 1983. FISHER, M. Toxocara cati: underestimaded zoonotic agent. Trends in Parasitology v. 19 n. 4, 2003. GAMBOA, M. I. Effects of temperatureand humidity on the development of eggs of Toxocara canis under laboratory conditions. Journal of Helminthology, v.79, p.327-331, 2005. HABLUETZEL, A.; TRALDI, G.; RUGGIERI, S., ATTILI, A. R., SCUPPA, P., ARCHETTI, R., MENGHINI, G.; ESPOSITO, F. An estimation of Toxocara canis prevalence in dogs, environmental egg contamination and risk of human infection in the March? region of Italy. Veterinary Parasitology, v.113, p.243-252, 2003. KAMIYA, M.; OOI, H. K.; NOMURA, T. The effect of radiation on the viability and migratory ability of second-stage larvae of Toxocara canis in mice. Veterinary parasitology, v.24, n.1, p.87-92, 1987. LACIAK, V.; LACIAKOV?, A.; M?T?, D.; SEVERA, J.; PAG??, M. Action of selected disinfectants on Toxocara canis eggs. Medycyna Weterynaryjna, v.65, n.2, p.102-106, 2009. LESCANO, S. A. L., CHIEFFI, P. P.; PERES, B. A.; VALARDE, C. N.; SALINAS, A. A.; ROJAS, C. E. Soil Contaminationa and Human infection by Toxocara sp. In the Urban Area of Lima, Peru. Memorial do Instituto Oswaldo Cruz, v.93, n.6, p.733-734, 1998. LESCANO, S. L.; QUEIROZ, M. L.; CHIEFFI, P. P. Larval recovery of Toxocara canis in Organs and Tissues of Experimentally Rattus norvegicus. Mem?rias do Instituto Oswaldo Cruz, v.99, n.6, p.627-628, 2004. LEVINE, N.D. Textbook of veterinary Parasitology. Minneapolis: Burges, 1978. 236p. 36 LUTY, T. Prevalence of Toxocara species in dogs, cats and red foxes from de Poznam region, Poland. Journal of Helminthology. v. 75, p. 153-156, 2001. MARTINEZ-BARBABOSA, I.; TSUJI, O. V.; CABELLO, R. R.; CARDEN?S, E. M. G.; CHASIN, O. A.. The prevalence of Toxocara cati in domestic cats in M?xico city. Veterinary Parasitology, v. 114, p. 43-49, 2003. MORRONDO, P.; D?EZ-MORRONDO, C.; PEDREIRA, J.; D?EZ-BA?OS, N.; S?NCHEZ-ANDRADE, R.; PAZ-SILVA, A.; D?EZ-BA?OS, P. Toxocara canis larvae viability after disinfectant-exposition. Parasitology Research, v.99, p.558-561, 2006. OLIVEIRA-SEQUEIRA, T. C. G.; AMARANTE, A. F. T.; FERRARI, T. B.; NUNES, L. C. Prevalence of intestinal parasites in dogs from S?o Paulo State, Brazil. Veterinary Parasitology, v.103, p.19-27, 2002. OLIVEIRA, C. A. F.; GERMANO P. M. L.; Estudo da ocorr?ncia de enteroparasitas em hortali?as comercializadas na regi?o metropolitana de S?o Paulo, SP, Brasil. I - Pesquisa de helmintos. Revista Sa?de P?blica, v. 26, n.4, p. 289-92 O?LORCAIN, P. The effect of freezing on the viability of Toxocara canis and T. cati embryonated eggs. Journal of Helminthology. v.69, p.169-171, 1995. OOI, H. K.; LIN, C. L.; WANG, J. S. Effect of ozone treatment on Toxocara canis eggs. Journal of Veterinary Medical Science, v.60, p.169-173, 1998. OSHIMA, T. Standarization of Techniques for infecting mice with Toxocara canis and observations on the normal migration routes of larvae. The Journal of Parasitology, v.47, p.657-660, 1961. OVERGAAUW, P. A. M. Aspects of Toxocara Epidemiology: Toxocarosis in Dogs and Cats. Critical Reviews in Microbiology, v.23, n.3, p.233-251, 1997. 37 PARSONS, J. C. Ascarid infections of cats and dogs. The Veterinary Clinics of North America, v.17, p.1307-1313, 1987. SAKAI, R.; KAWASHIMA, H.; SHIBUI, H.; KAMATA, K.; KAMBARA, C.; TOCHIGI,H. M. Toxocara cati. Induced Ocular Toxocariasis. Archive Ophthalmology. Japan, v.116, p.1686-1687, 1998. SERRA, C. M. B.; UCH?A, C. M. A.; COIMBRA, R. A. Exame parasitol?gico de fezes de gato (Felis catus domesticus) domiciliados e errantes da regi?o metropolitana do Rio de Janeiro, Brasil. Revista da Sociedade Brasileira de Medicina Tropical, v. 36, n.3, p.331-334, 2003. SOMMERFELT, I.E; ROSA, A.; DUCHENE, A.; DEGREGORIO, A.; LO?PEZA, C.; PISAN?, A.; DE TORRES, R. Toxocara canis in experimentally infected pigs: migratory pattern and tissue lesions. Veterinary Parasitology, v. 125, p.323?334, 2004. SOULSBY, E. J. L. Parasitolog?a y enfermedades parasitarias en los animales dom?sticos. 7 ed., M?xico: Interamericana, 1987. 823p. SPRENT, J. F. A. The life history and development of Toxocara cati (Schrank 1788) in the domestic cat. Parasitology, v.46, n.1-2, p.54-78, 1956. SPRENT, J. F. A. Observations on the development of Toxocara canis (Werner, 1782) in the dog. Parasitology, v.48, p.184-209, 1958. SWERCZEK, T.W.; NIELSEN, S. W.; HELMBOLDT, C. F. Transmammary passage of Toxocara cati in the cat. American Journal of Veterinary Research, v.32, p.89?92, 1971. VAN KNAPEN, F.; FRANCHIMONT, J. H. Steam sterilisation of sandpits infected with Toxocara eggs. British Medical Journal, v.1, p.1320, 1979. 38 VEROCAI, G.G.; TAVARES, P.V.; RIBEIRO, F. A.; CORREIA, T. R.; SCOTT, F. B. Effects of disinfectants on Toxocara canis embryogenesis and larval establishment in mice tissues. Zoonoses and Public Health, v.57, n.1, p. 213-216, 2010. VIDAL, J. E.; SZTAJNBOK, J.; SEGURO, A. C. Eosinophilic meningoencephalitis due to Toxocara canis: Case report and Review of the Literature. American Journal of Tropical Medicine and Hygiene, v.60, n.3, p.341-343, 2003. YANG, J.; KEYSTONE, J. S.; MCINTYRE, L.; SPENCE, H. Toxocara Antibodies in veterinary Personnel. Canine Veterinary Journal, v.23, p. 126-128, 1982. WANG, G. X.; LUO, Z. J. A novel method for the recovery of Toxocara canis in mice. Journal of Helminthology, v.72, p.183-184, 1998. ZAR, J.H. Biostatistical Analysis. 4a ed, Prentice Hall, New Jersey, 1999. ZIBAEI, M.; SADJJADI, S. M.; UGA, S.; Experimental Toxocara cati infection in Gerbils and Rats. 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dc.title.por.fl_str_mv |
A??o de diferentes desinfetantes na viabilidade e desenvolvimento de ovos e na migra??o larvar de Toxocara cati (Schrank,1788) em camundongos |
dc.title.alternative.eng.fl_str_mv |
Action of different disinfectants on the viability and development of eggs and in the migration of larvae of Toxocara cati (Schrank, 1788) in mice |
title |
A??o de diferentes desinfetantes na viabilidade e desenvolvimento de ovos e na migra??o larvar de Toxocara cati (Schrank,1788) em camundongos |
spellingShingle |
A??o de diferentes desinfetantes na viabilidade e desenvolvimento de ovos e na migra??o larvar de Toxocara cati (Schrank,1788) em camundongos Tavares, Pedro Vianna Desinfetantes Toxocara cati desenvolvimento Disinfectants Toxocara cati development Medicina Veterin?ria |
title_short |
A??o de diferentes desinfetantes na viabilidade e desenvolvimento de ovos e na migra??o larvar de Toxocara cati (Schrank,1788) em camundongos |
title_full |
A??o de diferentes desinfetantes na viabilidade e desenvolvimento de ovos e na migra??o larvar de Toxocara cati (Schrank,1788) em camundongos |
title_fullStr |
A??o de diferentes desinfetantes na viabilidade e desenvolvimento de ovos e na migra??o larvar de Toxocara cati (Schrank,1788) em camundongos |
title_full_unstemmed |
A??o de diferentes desinfetantes na viabilidade e desenvolvimento de ovos e na migra??o larvar de Toxocara cati (Schrank,1788) em camundongos |
title_sort |
A??o de diferentes desinfetantes na viabilidade e desenvolvimento de ovos e na migra??o larvar de Toxocara cati (Schrank,1788) em camundongos |
author |
Tavares, Pedro Vianna |
author_facet |
Tavares, Pedro Vianna |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Scott, Fabio Barbour |
dc.contributor.advisor1ID.fl_str_mv |
00138216797 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1217253540572819 |
dc.contributor.referee1.fl_str_mv |
Scott, Fabio Barbour |
dc.contributor.referee2.fl_str_mv |
Tancredi, Ian Philippo |
dc.contributor.referee3.fl_str_mv |
Martins, Isabella Vilhena Freire |
dc.contributor.authorID.fl_str_mv |
09801343745 |
dc.contributor.authorLattes.fl_str_mv |
xxxxxxxxxxxxxxxxx |
dc.contributor.author.fl_str_mv |
Tavares, Pedro Vianna |
contributor_str_mv |
Scott, Fabio Barbour Scott, Fabio Barbour Tancredi, Ian Philippo Martins, Isabella Vilhena Freire |
dc.subject.por.fl_str_mv |
Desinfetantes Toxocara cati desenvolvimento |
topic |
Desinfetantes Toxocara cati desenvolvimento Disinfectants Toxocara cati development Medicina Veterin?ria |
dc.subject.eng.fl_str_mv |
Disinfectants Toxocara cati development |
dc.subject.cnpq.fl_str_mv |
Medicina Veterin?ria |
description |
The Toxocara cati is one of the major intestinal parasites of cats and has great importance in public health as zoonotic agent of Visceral and Ocular Larva Migrans in humans. Studies to eliminate the infectious forms of this helminths are scarce. The aim of this study was to evaluate the action of various disinfectant solutions on the development of eggs of T. cati viability and infectivity of larvae present in the eggs of this parasite. Eggs were obtained from the dissection of female T. cati, recovered from infected cats. These were suspended in distilled water and divided into five groups with six replicates, each with about 3300 eggs. Each group was exposed for one hour at one of four selected commercial disinfectants and a control, and then washed with distilled water for removal of disinfectant solutions. All samples were assessed for development of eggs on days 0, +3, +6, +9, +12, +15, +18, +21 and +24. At the end of the observation period, 30 mice were infected with the samples. After 15 days the mice were euthanized and the brain, liver, lungs and kidneys, and carcass, macro-and microscopically observed. From day +6 eggs exposed to different treatments showed larva inside. And even after 24 days of exposure, the disinfectants were not able to inhibit the development or eliminate the larvae. The number of larvae recovered from infected mice showed that migration was observed in all animals. The carcass showed better recovery between the tissues analyzed. In no animal treated larvae were seen in the brain. No disinfectant used was able to inhibit embryogenesis of eggs of T. cati after one hour of exposure. The degree of infectivity and the migration pattern of the larvae was maintained even after treatment with disinfectants |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-03-30 |
dc.date.accessioned.fl_str_mv |
2020-08-13T14:11:27Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
TAVARES, Pedro Vianna. A??o de diferentes desinfetantes na viabilidade e desenvolvimento de ovos e na migra??o larvar de Toxocara cati (SCHRANK, 1788) em camundongos. 2011. 38 f. Disserta??o (Mestrado em Ci?ncias Veterin?rias) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2011. |
dc.identifier.uri.fl_str_mv |
https://tede.ufrrj.br/jspui/handle/jspui/3810 |
identifier_str_mv |
TAVARES, Pedro Vianna. A??o de diferentes desinfetantes na viabilidade e desenvolvimento de ovos e na migra??o larvar de Toxocara cati (SCHRANK, 1788) em camundongos. 2011. 38 f. Disserta??o (Mestrado em Ci?ncias Veterin?rias) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2011. |
url |
https://tede.ufrrj.br/jspui/handle/jspui/3810 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.references.por.fl_str_mv |
ABOUGRAIN, A. K.; NAHAISI, M. H.; MADI, N. S.; SAIED, M. M.; GHENGHESH, K. S. Parasitological contamination in salad vegetables in Tripoli-Libya. Food Control, v.21, p.760-762, 2010. ABO-SHEHADA, M. N.; HERBERT, I. V. The migration of larval Toxocara canis in mice II. Veterinary Parasitology, v.17, n.1, p.75-83, 1985. ACHA, P.; SZYFRES, B. Zoonosis y enfermedades transmissibles al hombre y a los animales, Organizaci?n Mundial de la Salud: Washington, 1986. AKAO, N.; TOMODA, M.; HAYASHI, E.; SIZUKI, R.; SHIMIZU-SUGANUMA, M.; SHICHINOHE, K.; FUJITA, K. Cerebellar ataxia due to Toxocara infection in Mongolian gerbils, Meriones unguiculatus. Veterinary Parasitology, v.113, p.229-237, 2003. ALBA-HURTADO, F.; MU?OZ-GUZM?N, M. A.; VALDIVIA-ANDA, G.; T?RTORA, J.L.; ORTEGA-PIERRES, M.G. Toxocara canis: Larval migration dynamics, detection of antibody reactivity to larval excretory?secretory antigens and clinical findings during experimental infection of gerbils (Meriones unguiculatus). Experimental Parasitology, v.122, n.1, p.1-5, 2009. AY?I?EK, H.; YARSAN, E.; SARIMEHMETOGLU, H. O.; TANY?KSEL, M.; GIRGINKARDESLER, N.; ?ZYURT, M. Efficacy of Some Disinfectants on Embryonated Eggs of Toxocara canis. Turkish Journal of Medical Science, v.31, n.1, p.35-39, 2001. AYRES M, AYRES J?NIOR M, AYRES DL, SANTOS AS. Bioestat 5.0: aplica??es estat?sticas nas ?reas das ci?ncias biol?gicas e m?dicas. Bel?m: Sociedade Civil Mamirau? 364p, 2007. 34 BARRIGA, O. O. A Critical Look at the Importance, Prevalence and Control of Toxocariasis and the Possibilities of Immunological Control. Veterinary Parasitology, v.29, n.2-3, p.195- 234, 1988. BEAVER, P. C.; SNYDER, C. H.; CARRERA, G. M.; DENT, J. H., LAFFERTY, J. W. Chronic eosinophilia due to visceral larva migrans: Report of three cases. Pediatrics, v.9, n.1, p.7-19, 1952. BOUCHET, F.; BOULLARD, Y.; BACCAM, D.; LEGER, N. Ultrastructural studies of alterations induced by microwaves in Toxocara canis eggs: prophylactic interest. Z. Parazitenkd, v.72, p.755-765, 1986. BOWMAN, D. D. Parasitologia Veterin?ria de Georgis. 8 ed. Barueri: Manole, 422p. 2006. CARDILLO, N.; ROSA, A.; RIBICICH, M.; L?PEZ, C.; SOMMERFELT, C. Experimental infection with Toxocara cati in BALB/c Mice, migratory behaviour and pathological changes. Zoonoses and Public Health, v.56, n.4, p.198-205, 2009. C?RTES V. A.; PAIM, G. V.; FILHO, R. A. A. Infesta??o por ancilostomideos e toxocarideos em c?es e gatos apreendidos em vias publicas, S?o Paulo. Revista Sa?de Publica, v. 22, n. 4, p. 341-3, 1988. DESPOMMIER, D. Toxocariasis: Clinical Aspects, Epidemiology, Medical Ecology, and Molecular Aspects. Clinical Microbiology Reviews, v.16, n.2, p.265-272, 2003. DUBEY, J. P. Migration of Toxocara cati larvae in mice. Tropical Geographic Medicine, v. 20, n. 2, p. 172-176, 1968. DUBINSKY, P.; HAVASIOVAREITEROVA, K.; PETKO, B.; HOVORKA, I.; TOMASOVICOVA, O. Role of small mammals in the epidemiology of toxocariasis. Parasitology, v.110, n.2, p.187?193, 1995. 35 DUNSMORE, J. D.; THOMPSON, R. C. A.; BATES, I. A. The accumulation of Toxocara canis larvae in the brains of mice. International Journal for Parasitology, v.13, n.5, p.517- 521, 1983. FISHER, M. Toxocara cati: underestimaded zoonotic agent. Trends in Parasitology v. 19 n. 4, 2003. GAMBOA, M. I. Effects of temperatureand humidity on the development of eggs of Toxocara canis under laboratory conditions. Journal of Helminthology, v.79, p.327-331, 2005. HABLUETZEL, A.; TRALDI, G.; RUGGIERI, S., ATTILI, A. R., SCUPPA, P., ARCHETTI, R., MENGHINI, G.; ESPOSITO, F. An estimation of Toxocara canis prevalence in dogs, environmental egg contamination and risk of human infection in the March? region of Italy. Veterinary Parasitology, v.113, p.243-252, 2003. KAMIYA, M.; OOI, H. K.; NOMURA, T. The effect of radiation on the viability and migratory ability of second-stage larvae of Toxocara canis in mice. Veterinary parasitology, v.24, n.1, p.87-92, 1987. LACIAK, V.; LACIAKOV?, A.; M?T?, D.; SEVERA, J.; PAG??, M. Action of selected disinfectants on Toxocara canis eggs. Medycyna Weterynaryjna, v.65, n.2, p.102-106, 2009. LESCANO, S. A. L., CHIEFFI, P. P.; PERES, B. A.; VALARDE, C. N.; SALINAS, A. A.; ROJAS, C. E. Soil Contaminationa and Human infection by Toxocara sp. In the Urban Area of Lima, Peru. Memorial do Instituto Oswaldo Cruz, v.93, n.6, p.733-734, 1998. LESCANO, S. L.; QUEIROZ, M. L.; CHIEFFI, P. P. Larval recovery of Toxocara canis in Organs and Tissues of Experimentally Rattus norvegicus. Mem?rias do Instituto Oswaldo Cruz, v.99, n.6, p.627-628, 2004. LEVINE, N.D. Textbook of veterinary Parasitology. Minneapolis: Burges, 1978. 236p. 36 LUTY, T. Prevalence of Toxocara species in dogs, cats and red foxes from de Poznam region, Poland. Journal of Helminthology. v. 75, p. 153-156, 2001. MARTINEZ-BARBABOSA, I.; TSUJI, O. V.; CABELLO, R. R.; CARDEN?S, E. M. G.; CHASIN, O. A.. The prevalence of Toxocara cati in domestic cats in M?xico city. Veterinary Parasitology, v. 114, p. 43-49, 2003. MORRONDO, P.; D?EZ-MORRONDO, C.; PEDREIRA, J.; D?EZ-BA?OS, N.; S?NCHEZ-ANDRADE, R.; PAZ-SILVA, A.; D?EZ-BA?OS, P. Toxocara canis larvae viability after disinfectant-exposition. Parasitology Research, v.99, p.558-561, 2006. OLIVEIRA-SEQUEIRA, T. C. G.; AMARANTE, A. F. T.; FERRARI, T. B.; NUNES, L. C. Prevalence of intestinal parasites in dogs from S?o Paulo State, Brazil. Veterinary Parasitology, v.103, p.19-27, 2002. OLIVEIRA, C. A. F.; GERMANO P. M. L.; Estudo da ocorr?ncia de enteroparasitas em hortali?as comercializadas na regi?o metropolitana de S?o Paulo, SP, Brasil. I - Pesquisa de helmintos. Revista Sa?de P?blica, v. 26, n.4, p. 289-92 O?LORCAIN, P. The effect of freezing on the viability of Toxocara canis and T. cati embryonated eggs. Journal of Helminthology. v.69, p.169-171, 1995. OOI, H. K.; LIN, C. L.; WANG, J. S. Effect of ozone treatment on Toxocara canis eggs. Journal of Veterinary Medical Science, v.60, p.169-173, 1998. OSHIMA, T. Standarization of Techniques for infecting mice with Toxocara canis and observations on the normal migration routes of larvae. The Journal of Parasitology, v.47, p.657-660, 1961. OVERGAAUW, P. A. M. Aspects of Toxocara Epidemiology: Toxocarosis in Dogs and Cats. Critical Reviews in Microbiology, v.23, n.3, p.233-251, 1997. 37 PARSONS, J. C. Ascarid infections of cats and dogs. The Veterinary Clinics of North America, v.17, p.1307-1313, 1987. SAKAI, R.; KAWASHIMA, H.; SHIBUI, H.; KAMATA, K.; KAMBARA, C.; TOCHIGI,H. M. Toxocara cati. Induced Ocular Toxocariasis. Archive Ophthalmology. Japan, v.116, p.1686-1687, 1998. SERRA, C. M. B.; UCH?A, C. M. A.; COIMBRA, R. A. Exame parasitol?gico de fezes de gato (Felis catus domesticus) domiciliados e errantes da regi?o metropolitana do Rio de Janeiro, Brasil. Revista da Sociedade Brasileira de Medicina Tropical, v. 36, n.3, p.331-334, 2003. SOMMERFELT, I.E; ROSA, A.; DUCHENE, A.; DEGREGORIO, A.; LO?PEZA, C.; PISAN?, A.; DE TORRES, R. Toxocara canis in experimentally infected pigs: migratory pattern and tissue lesions. Veterinary Parasitology, v. 125, p.323?334, 2004. SOULSBY, E. J. L. Parasitolog?a y enfermedades parasitarias en los animales dom?sticos. 7 ed., M?xico: Interamericana, 1987. 823p. SPRENT, J. F. A. The life history and development of Toxocara cati (Schrank 1788) in the domestic cat. Parasitology, v.46, n.1-2, p.54-78, 1956. SPRENT, J. F. A. Observations on the development of Toxocara canis (Werner, 1782) in the dog. Parasitology, v.48, p.184-209, 1958. SWERCZEK, T.W.; NIELSEN, S. W.; HELMBOLDT, C. F. Transmammary passage of Toxocara cati in the cat. American Journal of Veterinary Research, v.32, p.89?92, 1971. VAN KNAPEN, F.; FRANCHIMONT, J. H. Steam sterilisation of sandpits infected with Toxocara eggs. British Medical Journal, v.1, p.1320, 1979. 38 VEROCAI, G.G.; TAVARES, P.V.; RIBEIRO, F. A.; CORREIA, T. R.; SCOTT, F. B. Effects of disinfectants on Toxocara canis embryogenesis and larval establishment in mice tissues. Zoonoses and Public Health, v.57, n.1, p. 213-216, 2010. VIDAL, J. E.; SZTAJNBOK, J.; SEGURO, A. C. Eosinophilic meningoencephalitis due to Toxocara canis: Case report and Review of the Literature. American Journal of Tropical Medicine and Hygiene, v.60, n.3, p.341-343, 2003. YANG, J.; KEYSTONE, J. S.; MCINTYRE, L.; SPENCE, H. Toxocara Antibodies in veterinary Personnel. Canine Veterinary Journal, v.23, p. 126-128, 1982. WANG, G. X.; LUO, Z. J. A novel method for the recovery of Toxocara canis in mice. Journal of Helminthology, v.72, p.183-184, 1998. ZAR, J.H. Biostatistical Analysis. 4a ed, Prentice Hall, New Jersey, 1999. ZIBAEI, M.; SADJJADI, S. M.; UGA, S.; Experimental Toxocara cati infection in Gerbils and Rats. Korean Journal of Parasitology, v.48, n.4, p.331-333, 2010. |
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