Caracteriza??o de novos riz?bios isolados de ra?zes de cana-de-a??car

Matos, Gustavo Feitosa de

Caracteriza??o de novos riz?bios isolados de ra?zes de cana-de-a??car

Detalhes bibliográficos
Autor(a) principal:	Matos, Gustavo Feitosa de
Data de Publicação:	2017
Tipo de documento:	Dissertação
Idioma:	por
Título da fonte:	Biblioteca Digital de Teses e Dissertações da UFRRJ
Texto Completo:	https://tede.ufrrj.br/jspui/handle/jspui/2436
Resumo:	Recent molecular biology studies have indicated that bacteria of the genus Bradyrhizobium and Rhizobium may also play a role in the biological nitrogen fixation process (BNF) when associated with sugarcane. The use of Vigna unguiculata trap plants allowed obtaining a diverse collection of endophytic Bradyrhizobium spp. from sugarcane roots. The present work aimed to characterize a group of bacteria of the genus Bradyrhizobium from sugarcane roots, as well as to evaluate the ability of a representative strain of this group and an isolate of the genus Rhizobium to promote growth in this crop. To define the taxonomic position of the isolates of the group, phylogenetic analyzes were performed with the individual 16S rRNA, ITS, nodC and recA genes and multilocus sequence analysis (MLSA) involving four housekeeping genes (recA, dnaK, glnII and atpD), in four representative isolates (BR 10280T, BR 10266, BR 10555 and BR 10556). In addition, biochemical and morphophysiological tests were performed. Experiments in which the isolates P9-20 (BR 10280) (Bradyrhizobium sp.) and P5-2 (Rhizobium sp.) were inoculated in sugarcane seedlings were conducted in a greenhouse to evaluate the growth promoting effect of these isolates. Two harvests were performed at 30 and 75 days after transplanting (DAT). Among the analyzed variables were the budding speed index (BSI), dry mass and total nitrogen (N). Phylogenetic analyzes positioned the isolates in the superclade of B. japonicum in an independent branch close to B. huanghuaihaiense, a soybean micro-symbiont. Despite the proximity of the group under study to B. huanghuaihaiense, these isolates did not induce nodulation in Glycine max. In addition, unlike B. huanghuaihaiense, the new isolates induced nodule formation in Phaseolus vulgaris. In morphophysiological studies, significant differences were demonstrated between the representative isolates from this study and B. huanghuaihaiense. In the greenhouse experiment, an increase on BSI and root dry mass of the inoculated treatment with the P5-2 isolate was observed in the first harvest. In the second harvest, increments were found in the root dry mass and root volume, as well as in the total N of the roots and aerial part, in the treatment inoculated with P5-2, although no statistical difference was detected. These results of the characterization indicate that the sugarcane isolates of the genus Bradyrhizobium represent a new species of this genus. In relation to the greenhouse experiment, the isolate of the genus Rhizobium sp. presented potential as a growth promoter in the sugarcane crop.

Metadados do item

id	UFRRJ-1_4549143d797147f3c9f0d6a6260baea4
oai_identifier_str	oai:localhost:jspui/2436
network_acronym_str	UFRRJ-1
network_name_str	Biblioteca Digital de Teses e Dissertações da UFRRJ
repository_id_str
spelling	Baldani, Jos? Ivo538.864.458-87http://lattes.cnpq.br/0129530048505952Rouws, Luc Felicianus Marie014.563.806-56http://lattes.cnpq.br/4500797890789377Zilli, Jerri ?dsonMedici, Leonardo Oliveira602.783.683-01http://lattes.cnpq.br/0129530048505952Matos, Gustavo Feitosa de2018-09-13T17:03:38Z2017-02-23MATOS, Gustavo Feitosa de. Caracteriza??o de novos riz?bios isolados de ra?zes de cana-de-a??car. 2017. 57 f. Disserta??o (Mestrado em Fitotecnia) - Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2017.https://tede.ufrrj.br/jspui/handle/jspui/2436Recent molecular biology studies have indicated that bacteria of the genus Bradyrhizobium and Rhizobium may also play a role in the biological nitrogen fixation process (BNF) when associated with sugarcane. The use of Vigna unguiculata trap plants allowed obtaining a diverse collection of endophytic Bradyrhizobium spp. from sugarcane roots. The present work aimed to characterize a group of bacteria of the genus Bradyrhizobium from sugarcane roots, as well as to evaluate the ability of a representative strain of this group and an isolate of the genus Rhizobium to promote growth in this crop. To define the taxonomic position of the isolates of the group, phylogenetic analyzes were performed with the individual 16S rRNA, ITS, nodC and recA genes and multilocus sequence analysis (MLSA) involving four housekeeping genes (recA, dnaK, glnII and atpD), in four representative isolates (BR 10280T, BR 10266, BR 10555 and BR 10556). In addition, biochemical and morphophysiological tests were performed. Experiments in which the isolates P9-20 (BR 10280) (Bradyrhizobium sp.) and P5-2 (Rhizobium sp.) were inoculated in sugarcane seedlings were conducted in a greenhouse to evaluate the growth promoting effect of these isolates. Two harvests were performed at 30 and 75 days after transplanting (DAT). Among the analyzed variables were the budding speed index (BSI), dry mass and total nitrogen (N). Phylogenetic analyzes positioned the isolates in the superclade of B. japonicum in an independent branch close to B. huanghuaihaiense, a soybean micro-symbiont. Despite the proximity of the group under study to B. huanghuaihaiense, these isolates did not induce nodulation in Glycine max. In addition, unlike B. huanghuaihaiense, the new isolates induced nodule formation in Phaseolus vulgaris. In morphophysiological studies, significant differences were demonstrated between the representative isolates from this study and B. huanghuaihaiense. In the greenhouse experiment, an increase on BSI and root dry mass of the inoculated treatment with the P5-2 isolate was observed in the first harvest. In the second harvest, increments were found in the root dry mass and root volume, as well as in the total N of the roots and aerial part, in the treatment inoculated with P5-2, although no statistical difference was detected. These results of the characterization indicate that the sugarcane isolates of the genus Bradyrhizobium represent a new species of this genus. In relation to the greenhouse experiment, the isolate of the genus Rhizobium sp. presented potential as a growth promoter in the sugarcane crop.Estudos recentes de biologia molecular indicaram que bact?rias do g?nero Bradyrhizobium e Rhizobium tamb?m podem ter um papel no processo de fixa??o biol?gica de nitrog?nio (FBN) quando associadas ? cana-de-a??car. O uso de plantas de Vigna unguiculata como ?isca? possibilitou a obten??o de uma cole??o diversa de Bradyrhizobium spp. endof?ticos a partir de ra?zes de cana-de-a??car. O presente trabalho teve como objetivo caracterizar um grupo de bact?rias do g?nero Bradyrhizobium proveniente de ra?zes de cana-de-a??car, assim como avaliar a capacidade de uma estirpe representante desse grupo e de um isolado do g?nero Rhizobium em promover efeito de crescimento nessa cultura. Para definir o posicionamento taxon?mico dos isolados do grupo, an?lises filogen?ticas foram realizadas com os genes individuais 16S rRNA, ITS, nodC e recA e an?lise de sequ?ncia de multilocus (MLSA) envolvendo quatro genes ?housekeeping? (recA, dnaK, glnII e atpD), em quatro isolados representantes (BR 10280T, BR 10266, BR10555 e BR 10556). Al?m disso, foram realizados testes bioqu?micos e morfofisiol?gicos. Experimento onde os isolados P9-20 (BR 10280) (Bradyrhizobium sp.) e P5-2 (Rhizobium sp.) foram inoculados em minitoletes de cana-de-a??car foi conduzido em casa de vegeta??o. Foram realizadas coletas aos 30 e 75 dias ap?s transplantio (DAT). Entre as vari?veis analisadas est?o o ?ndice de velocidade de brotamento (IVB), massa seca e Nitrog?nio (N) total. An?lises filogen?ticas posicionaram os isolados no super clado de B. japonicum em um ramo independente pr?ximo a B. huanghuaihaiense, um microssimbionte de soja. Apesar da proximidade do grupo em estudo com B. huanghuaihaiense, estes isolados n?o induziram nodula??o em Glycine max. Al?m disso, diferente de B. huanghuaihaiense, os novos isolados induziram a forma??o de n?dulos em Phaseolus vulgaris. Em estudos morfofisiol?gicos foram demostradas diferen?as significativas entre os isolados representantes do grupo em estudo e B. huanghuaihaiense. Em experimento em casa de vegeta??o, observou-se na primeira coleta um incremento sobre o IVB e massa seca da raiz no tratamento inoculado com o isolado P5-2, embora n?o tenham sido observadas diferen?as estat?sticas. Na segunda coleta, incrementos foram encontrados na massa seca de raiz e volume de raiz, assim como, no N total das ra?zes e parte a?rea, no tratamento incoculado com P5-2, embora n?o tenham apresentado diferen?a estat?stica. Massa seca da parte a?rea do tratamento inoculado com P5-2 apresentou incremento na primeira e segunda coleta, em rela??o aos controles, embora n?o tenha sido detectada diferen?a estat?stica. Os resultados da caracteriza??o do grupo indicam que os isolados de cana-de-a??car do g?nero Bradyrhizobium representam uma nova esp?cie desse g?nero. Em rela??o ao experimento em casa de vegeta??o, o isolado do g?nero Rhizobium sp. apresentou potencial como promotor de crescimento na cultura de cana-de-a??carSubmitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2018-09-13T17:03:37Z No. of bitstreams: 1 2017 - Gustavo Feitosa de Matos.pdf: 1844017 bytes, checksum: 0ed6966082e21d15123a12cd0e5777ef (MD5)Made available in DSpace on 2018-09-13T17:03:38Z (GMT). No. of bitstreams: 1 2017 - Gustavo Feitosa de Matos.pdf: 1844017 bytes, checksum: 0ed6966082e21d15123a12cd0e5777ef (MD5) Previous issue date: 2017-02-23CNPq - Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gicoapplication/pdfhttps://tede.ufrrj.br/retrieve/53640/2017%20-%20Gustavo%20Feitosa%20de%20Matos.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em FitotecniaUFRRJBrasilInstituto de AgronomiaALMEIDA, N. F.; YAN, S. C.; CAI, R. M.; CLARKE, C. R.; MORRIS, C. E.; SCHAAD, N. W.; SCHUENZEL, E. L.; LACY, G. H.; SUN, X. A., JONES, J. B.; CASTILLO, J. A.; BULL, C. T.; LEMAN, S.; GUTTMAN, D. S.; SETUBAL, J. C.; VINATZER, B. A. A multilocus sequence typing and analysis database and website for plant-associated Microbes. Phytopathology, v. 100, n. 3, p. 208-215, 2010. ALTSCHUL, S.F.; MADDEN, T.L.; SCH?FFER, A.A.; ZHANG, J.; ZHANG, Z.; MILLER, W.; AND LIPMAN, D.J. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res, v. 25, n. 17, p. 3389-3402, 1997. AMANN, R. AND W. LUDWIG. Ribossoma RNA-targeted nucleic acid probes for studies in microbial ecology. FEMS Microbiology Reviews, v. 24, n. 5, p. 555-565, 2000. ANDO, S.; GOTO, M.; MEUNCHANG S.; THONGRA-AR, P.; FUJIWARA, T.; HAYASHI, H.; YONEYAMA, T. Detection of nifH sequences in sugarcane (Saccharum officinarum L.) and pineapple (Ananas comosus [L.] Merr.). Soil Science & Plant Nutrition, v. 51, n. 2, p. 303-308, 2005. ARAUJO, E.O.; ROCHA, J. R.; GEROLA, J. G.; MATTE, L. C. Diazotrophic bacteria inoculation associates with acids and nitrogen in corn. African Journal of Plant Science, v. 10, n. 8, p. 162-166, 2016. BALDANI, J.I.; BALDANI, V.; SELDIN, L.; D?BEREINER, J. Characterization of Herbaspirillum seropedicae gen. nov., sp. nov., a root-associated nitrogen-fixing bacterium. International Journal of Systematic and Evolutionary Microbiology, v. 36, p. 86-93, 1986. BALDANI, J.I.; BALDANI, V.L.D.; SELDIN, L.; DOBEREINER, J. Characterization of Herbaspirillum seropedicae gen.nov., a root associated nitrogen fixing bacterium. International Journal of Systematic and Evolutionary Microbiology, v. 36, n. 1, p. 86-93, 1986. BALDANI, J.I.; CARUSO, L.; BALDANI, V.L.D.; GOI, S.R.; D?BEREINER, J. Recent advances in BNF with non-legume plants. Soil Biology and Biochemistry, v. 29, p. 911- 922, 1997. BALDANI, J.I.; POT, B.; KIRCHHOF, G.; FALSEN, E.; BALDANI, V.L.D.; OLIVARES, F.L.; HASTE, B.; KERSTERS, K.; HORTMANN, A.; GILLIS, M.; DOBEREINER, J. Emended description of Herbaspirillum, inclusion of (Pseudomonas) rubrisubalbicans, a mild plant pathogen, as Herbaspirillum rubrisubalbicans comb. nov.; and classification of a group of clinical isolates (EF group 1) as Herbaspirillum species 3. International Journal of Systematic and Evolutionary Microbiology, v. 46, n. 3, p. 802-810, 1996. BALDANI, J.I.; REIS, V.M.; VIDEIRA, S.S.; BODDEY, L.H.; BALDANI, V.L.D. The art of isolating nitrogen-fixing bacteria from non-leguminous plants using N-free semi-solid media: a practical guide for microbiologists. Plant and Soil, v. 384, p. 413-431, 2014. 40 BALDANI, V.L.D.; BALDANI, J.I.; D?BEREINER, J. Inoculation of rice plants with the endophytic diazotrophs Herbaspirillum seropedicae and Burkholderia spp.. Biology and Fertility of Soils, v. 30, n. 5, p. 485-491, 2000. BEIJERINCK, M.W. Cultur des Bacillus radicola aus den Kno llchen. Bot. Ztg., v. 46, p. 740?750, 1888. BENEDUZI, A.; MOREIRA, F.; COSTA, P.B.; VARGAS, L.K.; LISBOA, B.B.; FAVRETO, R. Diversity and plant growth promoting evaluation abilities of bacteria isolated from sugarcane cultivated in the South of Brazil. Applied Soil Ecology, v.63, p. 94?104, 2013. BHATTACHARJEE, R.B.; AQBAL, S.; MUKHOPADHYAY,S.N. Use of nitrogen?fixing bacteria as biofertiliser for non?legumes: prospects and challenges. Applied Microbiology and Biotechnology, v.80, p.199?209, 2008. BHATTACHARYYA, P.N.; JHA, D.K. Plant growth-promoting rhizobacteria (PGPR): emergence in agriculture. World Journal of Microbiology and Biotechnology, v. 28, p. 1327?1350, 2012. BOMAR, L.; MALTZ, M.; COLSTON, S.; GRAF, J. Direct culturing of microorganisms using metatranscriptomics. MBio, v. 2, n. 2, p. e00012-11, 2011. BROUGHTON, W.J.; HANIN, M.; RELIC, B.; KOPCINSKA, J.; GOLINOWSKI, W.; SIMSEK, S.; OJANEN-REUHS, T.; REUHS, B.; MARIE, C.; KOBAYASHI, H.; BORDOGNA, B.; LE QUERE, A.; JABBOURI, S.; FELLAY, R.; PERRET, X.; DEAKIN, W.J. Flavonoid-inducible modifications to rhamnan O antigens are necessary for Rhizobium sp. Strain NGR234- Legume Symbioses. Journal of Bacteriology, v. 188, n. 10, p. 3654-3663, 2006. BURBANO, C.S.; LIU, Y.; R?SNER, K.L.; REIS, V.M.; CABALLERO-MELLADO, J.; REINHOLD-HUREK, B.; HUREK T. Predominant nifH transcript phylotypes related to Rhizobium rosettiformans in field-grown sugarcane plants and in Norway spruce. Environmental microbiology reports, v. 3, p. 383-389, 2011. CARDINALE, M.; BRUSETTI, L.; QUATRINI, P. Comparison of different primer sets for use in automated ribosomal intergenic spacer analysis of complex bacterial communities. Applied Microbiology and Biotechnology, v. 70, p. 6147?56, 2004. CAVALCANTE, V.A.; DOBEREINER, J.A. New acid-tolerant nitrogenfixing bacterium associated with sugar-cane. Plant and Soil, v. 108, p. 23-31, 1988. CHAHBOUNE, R.; CARRO, L.; PEIX, A.; RAM?REZ-BAHENA, M.H.; BARRIJAL, S.; VEL?ZQUEZ, E.; BEDMAR, E. J. Bradyrhizobium rifense sp. nov. isolated from effective nodules of Cytisus villosus grown in the Moroccan Rif. Systematic and Applied Microbiology, v.35, p.302-305, 2012. CHAINTREUIL, C.; GIRAUD, E.; PRIN, Y.; LORQUIN, J.; B?, A.; GILLIS, M.; LAJUDIE, P.; DREYFUS, B. Photosynthetic Bradyrhizobia are natural endophytes of the African wild rice Oryza breviligulata. Applied and Environmental Microbiology, v. 66, p. 5437?5447, 2000. 41 CHAVES, V.; SANTOS, S.; SCHULTZ, N.; PEREIRA, W.; SOUSA, J.; MONTEIRO, R.; REIS, V. Desenvolvimento Inicial de Duas Variedades de Cana-de-a??car Inoculadas com Bact?rias Diazotr?ficas. Revista Brasileira Ci?ncia do Solo, Vi?osa-MG, v. 39, n. 6, p. 1595-1602, 2015. CHRISTENSEN, H.; KUHNERT, P.; BUSSE, H-J.; FREDERIKSEN, W.C.; BISGAARD,M. Proposed minimal standards for the description of genera, species and subspecies of the Pasteurellaceae. International Journal of Systematic Bacteriology, v. 57, p. 166-178, 2007. COUTINHO, H.L.C.; OLIVEIRA, V.M.; MANFIO, G.P.; ROSADO, A.S. Evoluating the microbial diversity of soil samples: methodological innovations. Anais da Academia de Ci?ncia, v.71, p.491-503, 1999. DALL?AGNOL, R.F.; RIBEIRO, R.A.; DELAMUTA, J.R.M.; ORMENO-ORRILLO, E.; ROGEL, M.A.; ANDRADE, D.S.; MART?NEZ-ROMERO, E.; HUNGRIA, M. Rhizobium paranaense sp. nov., an effective N2-fixing symbiont of common bean (Phaseolus vulgaris L.) with broad geographical distribution in Brazil. International Journal of Systematic and Evolutionary Microbiology, v. 64, p. 3222-3229, 2014. DALL?AGNOL, R.F.; DELAMUTA, J.R.M.; RIBEIRO, A.R.; HUNGRIA, M. Diversidade e filogenia de estirpes de riz?bios pela metedologia de MLSA. Embrapa Soja-Artigo em anais de congresso. responsabilidade socioambiental da pesquisa agr?cola: anais. Vi?osa: SBCS. 2014.4. Trab.1212, 2012. DAVIS, K.E.R.; SANGWAN, P.; JANSSEN, P.H. Acidobateria, Rubrobacteridae and Chloroflexi are abundant among very slow-growing and mini-colony-forming soil bacteria. Environmental microbiology, v. 13, n. 3, p. 798-805, 2011. DELAMUTA, J.R.M.; RIBEIRO, R.A.; ORME?O-ORRILLO, E.; MELO, I.S.; MARTI?NEZ-ROMERO, E.; HUNGRIA, M. Polyphasic evidence supporting the reclassification of Bradyrhizobium japonicum group Ia strains as Bradyrhizobium diazoefficiens sp. nov. International Journal of Systematic and Evolutionary Microbiology, v. 63, p. 3342?3351, 2013. D?NARI?, J.; DEBELL?, F.; PROM?, J.C. Rhizobium lipochitooligosaccharide nodulation factors: signalling molecules mediating recognition and morphogenesis. Annual Review Biochemistry, Palo Alto, v. 65, p. 503- 535, 1996. DIXON, R. AND KAHN, D. Genetic regulation of biological nitrogen fixation. Nature Reviews Microbiology, v. 2, n. 8, p. 621-631, 2004. DOBBELAERE, S.; VANDERLEYDEN, J.; OKON, Y. Plant growth-promoting effects of diazotrophs in the rhizosphere. Critical Reviews in Plant Sciences, v. 22, p. 107?149, 2003. DOBEREINER J.; RUSCHEL A. P. Uma nova esp?cie de Beijerinckia. Revista de Biologia, v. 1, p. 261-272, 1958. D?BEREINER, J. Nitrogen-fixing bacteria of the genus Beijerinckia Derx in the rhizosphere of sugar cane. Plant and Soil, v. 15, p. 211, 1961. 42 D?BEREINER, J. Recent changes in concepts of plant bacteria interactions: Endophytic Nz fixing bacteria. Ci?ncia e Cultura, v. 44, p. 310-313, 1992. DOBEREINER, J.; BALDANI, J.I.; BALDANI, V.L.D. Como isolar e identificar bact?rias diazotr?ficas de plantas n?o leguminosas. Embrapa SPI, 1995. EDGAR, R.C. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic acids research, v. 32, p. 1792-1797, 2004. FELSENSTEIN, J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution, p. 783-791, 1985. FERRARA, F.I.; OLIVEIRA, Z.M.; GONZALES, H.H.S.; FLOH, E.I.S.; BARBOSA, H.R. Endophytic and rhizospheric enterobacteria isolated from sugar cane have different potentials for producing plant growthpromoting substances. Plant and Soil, v. 353, p. 409?417, 2012. FERREIRA, D.F. Sisvar: a Guide for its Bootstrap procedures in multiple comparisons. Ci?ncia e Agrotecnologia, v. 38, n. 2, p. 109-112, 2014. FISCHER, D.; PFITZNER, B.; SCHMID, M. et al. Molecular characterization of the diazotrophic bacterial community in uninoculated and inoculated field-grown sugarcane (Saccharum sp.). Plant and soil, v. 356, n. 1-2, p. 83-99, 2012. FRANK, B. Ueber die Pilzsymbiose der Leguminosen. Bulletin der Akademie der Wissenschaft in Erakau, 1889. FURUSHITA, M.; SHIBA, T.; MAEDA, T.; YAHATA, M.; KANEOKA, A.; TAKAHASHI, Y.; TRII, K.; HASEGAWA, T.; OHTA, M. Similarity of tetracycline resistance genes isolated from fish farm bacteria to those from clinical isolates. Applied and Environmental Microbiology, v. 69, p. 5336?5342, 2003. GALTIER, N.; GOUY, M.; GAUTIER, C. SEAVIEW and PHYLO_WIN: two graphic tools for sequence alignment and molecular phylogeny. Computer applications in the biosciences: CABIOS, v. 12, n. 6, p. 543-548, 1996. GARC A-MART NE , J.; ACINA , .G.; RODR GUE -VALERA, F. Use of the 16S?23S ribosomal genes spacer region in studies of prokaryotic diversity. Journal of Microbiological Methods, v. 36, p. 55-64, 1999. GEVERS, D.; COHAN, F.M.; LAWRENCE, J.G.; SPRATT, B.G.; COENYE, T.; FEIL, E.J.; STACKEBRANDT, E.; de PEER, Y.V.; VANDAMME, P.; THOMPSON, F.L.; SWINGS, J. Re-evaluating prokaryotic species. Nature Reviews Microbiology, v.3, p.733-739, 2005. GILLIS, M.; KERSTIRS, K.; HOSTE, B.; JANSSENS, D.; KROPPENSTEDT, R.M.; STEPHOM, M.P.; TEXEIRA, K.R.S.; DOBEREINER, J.; LEY, J. Acetobacter diazotrophicus sp.nov., a nitrogen fixing acetic acid bacterium associated with sugar cane. International Journal of Systematic Bacteriology, v. 39, p.361-364, 1989. G?RIO, L.A.S.; DIAS, F.L.F.; REIS, V.M.; URQUIAGA, S.; SCHULTZ, N.; BOLONHEZI, D.; MUTTON, M.A. Bact?rias promotoras de crescimento e aduba??o nitrogenada no crescimento inicial de cana-de-a??car proveniente de mudas pr?-brotadas. Pesquisa Agropecu?ria Brasileira, v. 50, p. 33-43, 2015. 43 GLICK, B.R. The enhancement of plant growth by free-living bacteria. Canadian Journal of Microbiology, v. 41, p. 109?117, 1995. GOMES, A.A.; REIS, V.M.; BALDANI, V.L.D.; GOI, S.R. Rela??o entre a distribui??o de nitrog?nio e coloniza??o por bact?rias diazotr?ficas em cana de a??car. Pesquisa Agropecu?ria Brasileira, v. 40, p. 1105-1113, 2005. GOVINDARAJAN, M.; BALANDREAU, J.; MUTHUKUMARASAMY, R.; REVATHI, G.; LAKSHMINARASIMHAN, C. Improved yield of micropropagated sugarcane following inoculation by endophytic Burkholderia vietnamiensis. Plant end Soil, v. 280, p. 239?252, 2006. GOVINDARAJAN, M.; KWON, S-W.; WEON, H-Y. Isolation, molecular characterization and growth-promoting activities of endophytic sugarcane diazotroph Klebsiella sp.. World Journal Microbiol, v. 23, p. 997?1006, 2007. GUTI?RREZ?MA?ERO, F.J.; RAMOS?SOLANO, B.; PROBANZA, A.; MEHOUACHI, J.; TADEO, F.; TALON, M. The plant?growth?promoting rhizobacteria Bacillus pumilus and Bacillus licheniformis produce high amounts of physiologically active gibberellins. Physiologia Plantarum, v. 111, p. 206-211, 2001. HALLMANN, J.; QUADT-HALLMANN, A.; MAHAFFEE, W. F.; KLOEPPER, J. W. Bacterial endophytes in agricultural crops. Canadian Journal of Microbiology. V. 43, p. 895-914, 1997. HARDOIM, P.R.; VAN OVERBEEK, L.S.; BERG, G.; PIRTTIL?, A.M.; COMPANT, S.; CAMPISANO, A.; DORING, M.; SESSITSCH, A. The hidden world within plants: ecological and evolutionary considerations for defining functioning of microbial endophytes. Microbiology and Molecular Biology Reviews, v. 79, p. 293-320, 2015. JORDAN, D.C. NOTES: transfer of Rhizobium japonicum Buchanan 1980 to Bradyrhizobium gen. nov., a genus of slow-growing, root nodule bacteria from leguminous plants. International Journal of Systematic and Evolutionary Microbiology, v. 32, n. 1, p. 136-139, 1982. KAMST, E.; ESPANHAK, H.P.; KAFETZOPOULOS, D. Biosynthesis and secretion of rhizobial lipochitin-oligosaccharide signal molecules. Subcellular Biochemistry, v. 29, p. 29-71, 1998. KAMST, E.; PILLING, J.; RAAMSDONK, L.M.; LUGTENBERG, B.J.J.; ESPANHAK, H.P. Rhizobium nodulation protein NodC is an important determinant of chitin oligosaccharide chain length in Nod factor biosynthesis, v.179, p.2103-2108, 1997. KANEKO, T.; NAKAMURA, Y.; SATO, S.; MINAMISAWA, K.; UCHIUMI, T.; SASAMOTO, S.; WATANABE, A.; IDESAWA, K.; IRIGUCHI, M.; KAWASHIMA, K.; KOHARA, M.; MATSUMOTO, M.; SHIMPO, S.; TSURUOKA, H.; WADA, T.; YAMADA, M.; TABATA, S. Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110. DNA Research, v. 9, p. 189-197, 2002. 44 KENNEDY, I.; CHOUDHURY, A.; KECSK?S, M. Non-symbiotic bacterial diazotrophs in crop-farming systems: can their potential for plant growth promotion be better exploited? Soil Biology and Biochemistry, v. 36, p. 1229?1244, 2004. KONSTANTINIDIS K.T.; TIEDJE J.M. Prokaryotic taxonomy and phylogeny in the genomic era: advancements and challenges ahead. Current opinion in microbiology, v. 10, p. 504-509, 2007. KONSTANTINIDIS, K.T.; RAMETTE, A.; TIEDJE, J.M. Toward a more robust assessment of intraspecies diversity, using fewer genetic markers. Applied and Environmental Microbiology, v. 72, p. 7286?7293, 2006. KUYKENDALL, L.D.; SAXENA, B.; DEVINE, T.E.; UDELL, S.E. Genetic diversity in Bradyrhizobium japonicum (Jordan 1982) and a proposal for Bradyrhizobium elkanii sp. nov. Canadian Journal of Microbiology, v. 38, n. 6, p. 501-505, 1992. LAGUERRE, G.; NOUR, S.M.; MACHERET, V.; SANJUAN, J.; DROUIN, P.; AMARGER, N. Classification of rhizobia based on nodC and nifH gene analysis reveals a close phylogenetic relationship among Phaseolus vulgaris symbionts. Microbiology, v. 147, p. 981?993, 2001. LANDELL, M.G.A.; CAMPANA, M.P., FIGUEIREDO, P.; XAVIER, M.A.; ANJOS, I.A.; DINARDO-MIRANDA, L.L.; SCARPARI, M.S.; GARCIA, J.C.; BID?IA, M.A.P.; SILVA, D.N.; MENDON?A, J,R,; KANTHACK, R.A.D.; CAMPOS, M.F.; BRANCALI?O, S.R.; PETRI, R.H.; MIGUEL, P.E.M. Sistema de multiplica??o de cana-de-a??car com uso de mudas pr?-brotadas (MPB), oriundas de gemas individualizadas. Ribeir?o Preto: Instituto Agron?mico de Campinas, 2012. LAWSON, D.M. & SMITH, B.E. Molybdenum Nitrogenases. In: Metals Ions in Biological System: Volume 39: Molybdenum and Tungsten: Their Roles in Biological Processes:. CRC Press, 2002. LEDGARD, S.F. & STEELE, K.W. Biological nitrogen fixation in mixed legume/grass pastures. Plant end Soil, v. 141, p. 137, 1992. LIMA, E.; BODDEY, R.M.; D?BEREINER, J. Quantification of biological nitrogen fixation associated with sugarcane using a 15N aided nitrogen balance. Soil Biology and Biochemistry, v. 19, n. 2, p. 165-170, 1987. LUDWIG, W.; STRUNK, O.; WESTRAM, R.; RICHTER, L.; MEIER, H.; YADHUKUMAR, A.; et al. ARB: a software environment for sequence data. Nucleic acids research, v. 32, n. 4, p. 1363-1371, 2004. MAGALH?ES, F.M.; BALDANI, J.I.; SOUTO, S.M.; KUYKENDALL, J.R.; D?BEREINER, J. A new acid-tolerant Azospirillum species. Anais-Academia Brasileira de Ciencias, 1983. MAGNANI, G.S.; DIDONET, C.M.; CRUZ, L.M.; PICHETH, C.F.; PEDROSA, F.O.; SOUZA, E.M. Diversity of endophytic bacteria in Brazilian sugarcane. Genetics and Molecular Research, v. 9, p. 250?258, 2010. 45 MAGUIRE, J.D. Speed of germination aid in selection and evaluation for seedling emergence and vigor. Crop Sci.;2:176-7, 1962. MARCELLETTI, S.; FERRANTE, P.; SCORTICHINI, M. Multilocus sequence typing reveals relevant genetic variation and different evolutionary dynamics among strains of Xanthomonas arboricola pv. juglandis. Diversity, v. 2, n. 11, p. 1205-1222, 2010. MARQUES J?NIOR, R.B.; CANELLAS, L.P.; SILVA, L.G.; OLIVARES, F.P. Promo??o de enraizamento de microtoletes de cana-de-a??car pelo uso conjunto de subst?ncias h?micas e bact?rias diazotr?ficas endof?ticas. Revista Brasileira de Ci?ncia do Solo, v. 32, p. 1121-1128, 2008. MATOS, G. F.; COELHO, M.R.R., BALDANI, J.I.; REIS, V.M.; URQUIAGA, S.; ROUWS, L.F.M. Positive effects os endophytic (Brady)rhizobium isolates on sugarcane sett germination and root growth under glass house conditions. In: 10th International PGPR WorKshop, Li?ge. From Omics to the Field, 2015. MATOS, G.F. Isolamento, caracteriza??o e aplica??o de riz?bios naturalmente associadas ? cana-de-a??car, usando novas estrat?gias de cultivo. Monografia do curso de gradua??o em Agronomia. Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, RJ, 39 p. 2015. MATTHEWS, S.S; SPARKES, D.L.; BULLARD, M.J. The response of wheat to inoculation with the diazotroph Azorhizobium caulinodans. Aspects Appl Biol, v. 63, p. 35?42, 2001. MENNA, P.; BARCELLOS, F.G.; HUNGRIA, M. Phylogeny and taxonomy of a diverse collection of Bradyrhizobium strains based on Multilocus Sequence Analysis of the 16S rRNA gene, ITS region and gln II, rec A, ATP D and dnaK genes. International Journal of Systematic and Evolutionary Microbiology, v. 59, p. 2934-2950. 2009. MOREIRA, F.M.S.; SILVA, K.; N?BREGA, R.S.A.; CARVALHO, F. Bact?rias diazotr?ficas associativas: diversidade ecologia e potencial de aplica??es. Comunicata Scientiae, v. 1, p. 74 ? 99, 2010. MOREIRA, F.M.S.; SIQUEIRA, J. O. Microbiologia e bioqu?mica do solo. 2. ed. Lavras: UFLA, p. 729, 2006. MULLIS, K.; FALOONA, F.; SCHARF, S.; SAIKI, R.; HORN, G.; ERLICH, H. Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. Cold Spring Harbor Symposia on Quantitative Biology, v. 51, p. 263-273, 1986. NAGPAL, M.L.; FOX, K.F.; FOX A. Utility of 16S?23S rRNA spacer region methodology: how similar are interspace regions within a genome and between strains for closely related organisms? Journal of Microbiological Methods, v. 33, 211?219, 1998. NOGUEIRA, A.R.A.; SOUZA, G.B. Manual de laborat?rio: solo, ?gua, nutri??o vegetal, nutri??o animal e alimentos. Embrapa Pecu?ria Sudeste, p. 313, 2005. NORRIS, D.O.; DATE, R.A. Legume bacteriology. Tropical Pastures Research; Principles and Methods. p, p. 134-174, 1976. 46 OLDROYD G.; SPEAK, F.; ENTER. Signalling systems that promote beneficial symbiotic associations in plants. Nature Reviews Microbiology, v. 11, p. 252?263, 2013. OLDROYD, G.E.; MURRAY, J.D.; POOLE P.S.; DOWNIE, J.A. The rules of engagement in the legume-rhizobial symbiosis. Annual Review of Genetics, v. 45, p. 119-44, 2011. OLIVARES, F.L.; JAMES, E.K.; BALDANI, J.I.; DOBEREINER, J. Infection of mottle strip disease-susceptible and resistant sugar cane varieties by the endophytc diazotroph Herbaspirillum. New phytologist, Cambridge, Gr?-Bretanha, v.135, p.575-597, 1997. OLIVEIRA, A.L.M.; CANUTO, E.L.; REIS, V.M.; BALDANI, J.I. Response of micropropagated sugarcane varieties to inoculation with endophytic diazotrophic bacteria. Brazilian Journal of Microbiology, v. 34, p. 59-61, 2003. OLIVEIRA, A.L.M.; URQUIAGA, S.; D?BEREINER, J. & BALDANI, J.I. The effect of inoculating endophytic N2 fixing bacteria on micro propagated sugarcane plants. Plant and Soil, v. 242, p. 205-215, 2002. OLIVEIRA, R.P.; SCHULTZ, N.; MONTEIRO, R.C.; PEREIRA, W.; DE ARA?JO, A.P.; URQUIAGA, S.; REIS, V.M. Growth analysis of sugarcane inoculated with diazotrophic bacteria and nitrogen fertilization. African Journal of Agricultural Research, v. 11, p. 2786-2795, 2016. ORME?O-ORRILLO, E.; RIBEIRO, R.A.; HELENE, L.C.F.; HUNGRIA, M.; DELAMUTA, J.R.M.; MART?NEZ-ROMERO, E.; ROGEL, M.A. Bradyrhizobium viridifuturi sp. nov., encompassing nitrogen-fixing symbionts of legumes used for greenmanure and environmental services. International Journal of Systematic and Evolutionary Microbiology. v. 65, p. 4441-4448, 2015. PAUNGFOO-LONHIENNE, C.; LONHIENNE, T.G.A.; YEOH, Y.K.; WEBB, R.I.; LAKSHMANAN, P.; CHAN, C.X.; LIM, P.E.; RAGAN, M.A.; SCHMIDT, S.; HUGENHOLTZ, P. A new species of Burkholderia isolated from sugarcane roots promotes plant growth. Microbial Biotechnology, v. 7, p. 142?154, 2014. PIROMYOU, P.T.; GREETATORN, K.; TEAMTISONG, T.; OKUBO, R.; SHINODA, A.; NUNTAKIJ, P.; TITTABUTR, N.; BOONKERD, K.; MINAMISAWA, AND N. TEAUMROONG. Preferential Association of Endophytic Bradyrhizobia with Different Rice Cultivars and Its Implications for Rice Endophyte Evolution. Applied and environmental microbiology, v. 81, n. 9, p. 3049-3061, 2015. QUECINE, M.C.; ARA?JO, W.L.; ROSSETTO, P.B.; FERREIRA, A.; TSUI, S.; LACAVA, P.T.; PIZZIRANI-KLEINER, A.A. Sugarcane Growth Promotion by the Endophytic Bacterium Pantoea agglomerans 33.1. Applied and Environmental Microbiology, v. 78, p. 7511?7518, 2012. R CORE TEAM. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. URL https://www.R-project.org/, 2016. REES, D.C. & HOWARD, J.B. Nitrogenase: standing at the crossroads. Current opinion in chemical biology, v. 4, n. 5, p. 559-566, 2000. 47 REINHOLD-HUREK, B. & HUREK, T. Living inside plants: bacterial endophytes. Current opinion in plant biology, v. 14, n. 4, p. 435-443, 2011. REINHOLD-HUREK, B.; HUREK, T. Life in grasses: diazotrophic endophytes. Trends in Microbiology v. 6, p. 139?144, 1998. REIS JUNIOR, F.B.; MENDES, I.E.; TEIXEIRA, K.R.S.; REIS, V.M. Uso de Ferramentas Moleculares em Estudos da Diversidade de Microrganismos do Solo. Planaltina, DF: Embrapa Cerrados, 2002. REIS JUNIOR, F.B; REIS, V.M.; URQUIAGA, S.; D?BEREINER, J. Influence of nitrogen fertilisation on the population of diazotrophic bacteria Herbaspirillum spp. and Acetobacter diazotrophicus in sugar cane (Saccharum spp.). Plant and Soil, v.219, p.153-159, 2000. REIS, F.B., SILVA, L.G.; REIS, V.M.; DOBEREINER, J. Occurrence of diazotrophic bacteria in different sugar cane genotypes. Pesquisa Agropecuaria Brasileira v. 35, p. 985? 994, 2000. REIS, V.M.; BALDANI, J.I.; URQUIAGA, S. Recomenda??o de uma mistura de estirpes de cinco bact?rias fixadores de nitrog?nio para inocula??o de cana-de-a??car: Gluconacetobacter diazotrophicus (BR 11281), Herbaspirillum seropedicae (BR 11335), Herbaspirillum rubrisubalbicans (BR 11504), Azospirillum amazonense (BR 11145) e Burkholderia tropica (BR11366). Embrapa Agrobiologia. Circular T?cnica, 2009. RICHARDSON, A.E.; BAREA, J.M.; MCNEILL, A.M. et al. Acquisition of phodphorus and nitrogen in the rhizosphere and plant growth promocion by mricroorganisms. Plant and Soil, v. 321, p. 305, 2009. RICHTER, M.; ROSSELL?-M?RA, R. Shifting the genomic gold standard for the prokaryotic species definition. Proceedings of the National Academy of Sciences, v. 106, p. 19126-19131, 2009. RIVAS, R.; MARTENS, M.; LAJUDIE, P.; WILLEMS, A. Multilocus sequence analysis of the genus Bradyrhizobium. Systematic and applied microbiology, v. 32, p. 101-110, 2009. RIVAS, R.; MARTENS, M.; LAJUDIE, P.; WILLEMS, A. Multilocus sequence analysis of the genus Bradyrhizobium. Systematic and applied microbiology, v. 32, p. 101-110, 2009. RIVAS, R.; WILLEMS, A.; PALOMO, J.L.; GARC?A-BENAVIDES, P.; MATEOS, P.F.; MART?NEZ-MOLINA, E.; GILLIS, M.; VEL?ZQUEZ, E. Bradyrhizobium betae sp. nov., isolated from roots of Beta vulgaris affected by tumour-like deformations. International Journal of Systematic Bacteriology, v. 54, p. 1271?1275, 2004. ROBERTSON, G.P.; VITOUSEK, P.M. Nitrogen in agriculture: balancing the cost of an essential resource. Annual Review of Environment and Resources, v. 34, p. 97?125, 2009. RODRIGUEZ NETO, J.; MALAVOLTA JUNIOR, V.A.; VICTOR, O. Meio Simples para isolamento e cultivo de Xanthomonas campestris pv. Citri tipo B. Summa Phytopathol, v. 12, n. 1-2, p. 32, 1986. RODRIGUEZ, H.; FRAGA, R. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnology advances, v. 17, p. 319-339, 1999. 48 ROESCH, L.F.; SELBACH, P.A.; S?, E.S. Reinocula??o de bact?rias diazotr?ficas aumentando o crescimento de plantas de trigo. Ci?ncia Rural, Santa Maria, v. 35, n.5, p. 1201-1204, 2005. ROSADO, A.S.; DUARTE, G.F.; MENDON?A-HAGLER, L.C. A moderna microbiologia do solo: Aplica??o de t?cnicas de biologia molecular. In: SIQUEIRA, J.D.; MOREIRA, F.M.S.; LOPES, A.S.; GUILHERME, LR.G.; FAQUIM, V.; FRUTINI-NETO, A.E.; CARVALHO, J.G. (Ed.). Inter-rela??o fertilidade, biologia do solo e nutri??o de plantas. Vi?osa: SBCS, Lavras: UFLA/DSC. p. 429-448, 1999. ROSENBLUETH, M. and MARTINEZ-ROMERO, E. Bacterial endophytes and their interactions with hosts. Molecular plant-microbe interactions, v. 19, n. 8, p. 827-837, 2006. ROSSEL?-MORA, R.; AMANN, R. The species concept for prokaryotes. FEMS Microbiology Review, v. 25, n. 1, p. 39-67, 2001. ROUWS, L.F.M.; LEITE, J.; MATOS, G.F.; ZILLI, J.E.; COELHO, M. R.R.; XAVIER, G. R.; FISCHER, D.; HARTMANN, A.; REIS, V.M., BALDANI, J.I. Endophytic Bradyrhizobium spp. isolates from sugarcane obtained through different culture strategies. Environmental microbiology reports, v. 6, n. 4, p. 354-363, 2013. RUSCHEL, A.P.; HENIS, Y.; SALATI E. Nitrogen-15 tracing of N-fixation with soil-grown sugarcane seedlings. Soil Biology and Biochemistry, v. 7, n. 2, p. 181-182, 1975. SAITOU, N.; NEI, M. The Neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, v. 4, p. 406-425, 1987. SALEH, S.A.; MEKHEMAR G.A.A.; EL-SOUD, A.A.A.; RAGAB, A.A.; MIKHAEEL, F.T. Survival of Azorhizobium and Azospirillum in different carrier materials: inoculation of wheat and Sesbania rostrata. Bulletin of Faculty of Agriculture, v. 52, p. 319?338, 2001. SANTI, C.; BOGUSZ, D.; FRANCHE, C. Biological nitrogen fixation in nonlegume plants. Annals of Botany, v. 111, p. 743?767, 2013. SANTOYO, G.; MORENO-HAGELSIEB, G.; OROZCO-MOSQUEDA, M.C.; GLICK, B.R, Plant growth-promoting bacterial endophytes. Microbiological Research, v. 183, p. 92?99, 2016. SARITA, S.; SHARMA, P.K.; PRIEFER, U.B.; PRELL, J. Direct amplification of rhizobial nodC sequences from soiltotal DNA and comparison to nodC diversity of root nodule isolates. FEMS microbiology ecology, v. 54, n. 1, p. 1-11, 2005. SCHULTZ, N.; SILVA, J.A.; SOUSA, J.S.; MONTEIRO, R.C.; OLIVEIRA, R.P.; CHAVES, V.A.; PEREIRA, W.; SILVA, M.; BALDANI, J.I.; BODDEY, R.M.; REIS, V.M.; URQUIAGA, S. Inoculation of sugarcane with diazotrophic bacteria. Revista Brasileira de Ci?ncia do Solo, v. 38, p. 407-414, 2014. SILVA, C., VINUESA, P., EGUIARTE, L.E., SOUZA, V., MART?NEZ-ROMERO, E. Evolutionary genetics and biogeographic structure of Rhizobium gallicum sensu lato, a widely 49 distributed bacterial symbiont of diverse legumes. Molecular Ecology, v. 14, p. 4033-4050, 2005. SOUZA, R.S.C.; OKURA, V.K.; ARMANHI, J.S.L.; JORR,?I.N.B.; LOZANO, N.; SILVA, M.J.; GONZ?ALEZ-GUERRERO, M.; ARAUJO, L.M.; FERREIRA, N.V.; BAGHERI, H.C.; IMPERIAL, J.; ARRUDA, P. Unlocking the bacterial and fungal communities assemblages of sugarcane microbiome. Scientific Reports v. 6, p. 28774, 2016. STACKEBRANDT, E.; GOEBEL, B. Taxonomic note. A place for DNA?DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. International Journal of Systematic and Evolutionary Microbiology, v. 44, n. 4, p. 846-849, 1994. STEPKOWSKI, T.; CZAPLINSKA, M., MIEDZINSKA, K.; MOULIN, L. The variable part of the dnaK gene as an alternative marker for phylogenetic studies of rhizobia and related alpha Proteobacteria. Systematic and Applied Microbiology, v.26, p.483-494, 2003. STEPKOWSKI, T.; MOULIN, L.; KRZYZANSKA, A.; MCINNES, A.; LAW, I.J.; HOWIESON, J. European origin of Bradyrhizobium populations infecting lupins and serradella in soils of western Australia and South Africa. Applied and Environmental Microbiology, v. 71, n. 11, p. 7041-7052, 2005. STEVENSON, B.S.; EICHORST, S.A.; WERTZ, J.T.; SCHMIDT, T.M.; BREZNAK, J.A. New strategies for cultivation and detection of previously uncultered microbes. Applied and Environmental Microbiology. v. 70, p. 4748-4755, 2004. TAMURA, K.; STECHER, G.; PETERSON, D.; FILIPSKI, A.; KUMAR, S. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution v. 30, p. 2725-2729, 2013. TEALE, W.; PAPONOV, I.; PALME, K. Auxin in action: signalling, transport and the control of plant growth and development. Nature Reviews Molecular Cell Biology, v. 7, n. 11, p. 847-859, 2006. THAWEENUT, N.; HACHISUKA, Y.; ANDO, S.; YANAGISAWA, S.; YONEYAMA, T. Two seasons study of nifH gene expression and nitrogen fixation by diazotrophic endophytes in sugarcane (Saccharum spp. hybrids): expression of nifH genes similar to those of rhizobia. Plant and Soil, v. 338, p. 435-449, 2011. TIGHE, S.W.; DE LAJUDIE, P.; DIPIETRO, K.; LINDSTR?M, K.; NICK, G.; JARVIS, B. D. Analysis of cellular fatty acids and phenotypic relationships of Agrobacterium, Bradyrhizobium, Mesorhizobium, Rhizobium and Sinorhizobium species using the Sherlock Microbial Identification System. International journal of systematic and evolutionary microbiology, v. 50, p. 787-801, 2000. TILMAN, D.; CASSMAN, K.G.; MATSON, P.A.; NAYLOR, R.; POLASKY, S. Agricultural sustainability and intensive production practices. Nature, v. 418, n. 6898, p. 671-677, 2002. 50 TOLEDO, B.F.B. Identifica??o de estirpes de riz?bios por sequenciamento parcial dos genes 16S rDNA e nifH. Graduate Program in Agronomy ? Plant Breeding and Genetics, UNESP, Jaboticabal, Brazil, 2008. UEDA, T.; SUGA, Y.; YAHIRO, N.; MATSUGUCHI, T. Phylogeny of Sym plasmids of rhizobia by PCR-based sequencing of a nodC segment. Journal of bacteriology, v. 177, n. 2, p. 468-472, 1995. URQUIAGA, S.; CRUZ, K.H.S.; BODDEY, R.M. Contribution of nitrogen fixation to sugar cane: nitrogen-15 and nitrogen-balance estimates. Soil Science Society of America Journal, v. 56, n. 1, p. 105-114, 1992. VAN BELKUM, A.; STRUELENS, M.; DE VISSER, A.; VERBRUGH, H.; TIBAYRENC, M. Role of Genomic Typing in Taxonomy, Evolutionary Genetics, and Microbial Epidemiology. Clinical Microbiology Reviews, v. 14, p. 547?560, 2001. VAN BERKUM, P.; TEREFEWORK, Z.; PAULIN, L.; SUOMALAINEN, S.; LINDSTROM, K.; EARDLY, B.D. Discordant phylogenies within the rrn loci of rhizobia. Journal of Bacteriology, v. 185, p. 2988-2998, 2003. VANDAMME, P.; POT, B.; GILLIS, M.; DEVOS, P.; KERSTERS, K.; SWING, S.J. Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbial Reviews, v. 60, p. 407?438, 1996. VESSEY, J.K. Plant growth promoting rhizobacteria as biofertilizers. Plant and Soil, v. 255, p. 571?586, 2003. VINCENT, J.M. A manual for the practical study of root-nodule bacteria., 1970. VINUESA, P.; LE?N-BARRIOS, M.; SILVA, C.; WILLEMS, A.; JARABO-LORENZO, A.; P?REZ-GALDONA, R.; WERNER, D.; MART?NEZ-ROMERO, E. Bradyrhizobium canariense sp. nov., an acid-tolerant endosymbiont that nodulates endemic genistoid legumes (Papilionoideae: Genisteae) from the Canary Islands, along with Bradyrhizobium japonicum bv. genistearum, Bradyrhizobium genospecies alpha and Bradyrhizobium genospecies beta. International Journal of Systematic Bacteriology, v. 55, p. 569?575, 2005. WANG, J.Y.; WANG, R.; ZHANG, Y.M.; LIU, H.C.; CHEN, W.F.; WANG, E.T., SUI, X. H.; CHEN, W.X. Bradyrhizobium daqingense sp. nov., isolated from soybean nodules. International journal of systematic and evolutionary microbiology, v. 63, p. 616-624, 2013. WANG, K.; YAN, P.S.; DING, Q.L.; WU, Q.X.; WANG, Z.B.; PENG, J. Diversity of culturable root-associated/endophytic bacteria and their chitinolytic and aflatoxin inhibition activity of peanut plant in China. World Journal of Microbiology and Biotechnology, v.29, p.1-10, 2013. WANG, Y.; BROWN, H.N.; CROWLEY, D.E.; SZANISZLO, P.J. Evidence for direct utilization of a siderophore, ferrioxamine B, in axenically grown cucumber. Plant, Cell & Environment, v. 16, n. 5, p. 579-585, 1993. WILLEMS, A. The taxonomy of rhizobia: an overview. Plant and Soil, v. 287, p. 3-14, 2006. 51 WILLEMS, A.; COOPMAN, R.; GILLIS, M. Phylogenetic and DNA-DNA hybridization analyses of Bradyrhizobium species. International journal of systematic and evolutionary microbiology v. 51, p. 111-117, 2001. WILLEMS, A.; DOIGNON-BOURCIER, F.; GORIS, J.; COOPMAN, R.; LAJUDIE, P.; DE VOS, P.; GILLIS, M. DNA?DNA hybridization study of Bradyrhizobium strains. International Journal of Systematic Bacteriology, v. 51, p. 1315?1322, 2001. WILLEMS, A.; MUNIVE, A.; DE LAJUDIE, P.; GILLIS, M. In most Bradyrhizobium groups sequence comparison of 16S-23S rDNA internal transcribed spacer regions corroborates DNA-DNA hybridizations. Systematic and Applied Microbiology, v. 26, p. 203?210, 2003. WOESE, C.R. Bacterial evolution. Microbiological reviews, v. 51, n. 2, p. 221, 1987. YANNI, Y.G.; RIZK, R.Y.; CORICH, V.; SQUARTINI, A.; NINKE, K.; PHILIPHOLLINGSWORTH, S.; ORGAMBIDE, G.; BRUINJ, F.; STOLTZFUS, J.; BUCKLEY, D.; SCHMIDT, T.M.; MATEOS, P.F.; LADHA, J.K.; DAZZO, F.B. Natural endophytic association between Rhizobium leguminosarum bv. trifolii and rice roots and assessment of its potential to promote rice growth. Plant and Soil v. 194, p. 99?114, 1997. YAO, Z.Y.; KAN, F.L.; WANG, E.T.; WEI, G.H.; CHEN, W.X. Characterization of rhizobia that nodulate legume species of the genus Lespedeza and description of Bradyrhizobium yuanmingense sp. nov. International Journal of Systematic and Evolutionary Microbiology, v. 52, p. 2219-2230, 2002. YAO, Z.Y.; KAN, F.L.; WANG, E.T.; WEI, G.H.; CHEN, W.X. Characterization of rhizobia that nodulate legume species within the genus Lespedeza and description of Bradyrhizobium yuanmingense sp. nov. International Journal of Systematic Bacteriology, v. 52, p. 2219?2230, 2002. YU, X.; CLOUTIER, S.; TAMBONG, J.T.; BROMFIELD, E.S. Bradyrhizobium ottawaense sp. nov., a symbiotic nitrogen fixing bacterium from root nodules of soybeans in Canada. International journal of systematic and evolutionary microbiology, v. 64, p. 3202-3207, 2014. ZAKHIA, F.; LAJUDIE, P. Taxonomy of rhizobia. Agronomie, v. 21, n. 6-7, p. 569-576, 2001. ZHANG, Y.M.; LI JR, Y.; CHEN, W.F.; WANG, E.T.; SUI, X.H.; LI, Q.Q.; ZHANG, Y.Z.; ZHOU, Y.G.; CHEN, W.X. Bradyrhizobium huanghuaihaiense sp. nov. an effective symbiotic bacterium isolated from soybean (Glycine max L.) nodules. International journal of systematic and evolutionary microbiology, v. 62, p. 1951-1957, 2012.Bradyrhizobiumtaxonomia de procariotospromo??o de crescimento em cana-de-a??carBradyrhizobiumtaxonomy of prokaryotesPromotion of growth in sugarcaneAgronomiaCaracteriza??o de novos riz?bios isolados de ra?zes de cana-de-a??carCharacterization of new rhizobia isolated from sugarcane rootsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRRJinstname:Universidade Federal Rural do Rio de Janeiro (UFRRJ)instacron:UFRRJTHUMBNAIL2017 - Gustavo Feitosa de Matos.pdf.jpg2017 - Gustavo Feitosa de Matos.pdf.jpgimage/jpeg1943http://localhost:8080/tede/bitstream/jspui/2436/18/2017+-+Gustavo+Feitosa+de+Matos.pdf.jpgcc73c4c239a4c332d642ba1e7c7a9fb2MD518TEXT2017 - Gustavo Feitosa de Matos.pdf.txt2017 - Gustavo Feitosa de Matos.pdf.txttext/plain163017http://localhost:8080/tede/bitstream/jspui/2436/17/2017+-+Gustavo+Feitosa+de+Matos.pdf.txtf5370c55f75ad879dce8a077ef22063fMD517ORIGINAL2017 - Gustavo Feitosa de Matos.pdf2017 - Gustavo Feitosa de Matos.pdfapplication/pdf1844017http://localhost:8080/tede/bitstream/jspui/2436/2/2017+-+Gustavo+Feitosa+de+Matos.pdf0ed6966082e21d15123a12cd0e5777efMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82089http://localhost:8080/tede/bitstream/jspui/2436/1/license.txt7b5ba3d2445355f386edab96125d42b7MD51jspui/24362023-04-14 15:43:05.103oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttps://tede.ufrrj.br/PUBhttps://tede.ufrrj.br/oai/requestbibliot@ufrrj.br\|\|bibliot@ufrrj.bropendoar:2023-04-14T18:43:05Biblioteca Digital de Teses e Dissertações da UFRRJ - Universidade Federal Rural do Rio de Janeiro (UFRRJ)false
dc.title.por.fl_str_mv	Caracteriza??o de novos riz?bios isolados de ra?zes de cana-de-a??car
dc.title.alternative.eng.fl_str_mv	Characterization of new rhizobia isolated from sugarcane roots
title	Caracteriza??o de novos riz?bios isolados de ra?zes de cana-de-a??car
spellingShingle	Caracteriza??o de novos riz?bios isolados de ra?zes de cana-de-a??car Matos, Gustavo Feitosa de Bradyrhizobium taxonomia de procariotos promo??o de crescimento em cana-de-a??car Bradyrhizobium taxonomy of prokaryotes Promotion of growth in sugarcane Agronomia
title_short	Caracteriza??o de novos riz?bios isolados de ra?zes de cana-de-a??car
title_full	Caracteriza??o de novos riz?bios isolados de ra?zes de cana-de-a??car
title_fullStr	Caracteriza??o de novos riz?bios isolados de ra?zes de cana-de-a??car
title_full_unstemmed	Caracteriza??o de novos riz?bios isolados de ra?zes de cana-de-a??car
title_sort	Caracteriza??o de novos riz?bios isolados de ra?zes de cana-de-a??car
author	Matos, Gustavo Feitosa de
author_facet	Matos, Gustavo Feitosa de
author_role	author
dc.contributor.advisor1.fl_str_mv	Baldani, Jos? Ivo
dc.contributor.advisor1ID.fl_str_mv	538.864.458-87
dc.contributor.advisor1Lattes.fl_str_mv	http://lattes.cnpq.br/0129530048505952
dc.contributor.advisor-co1.fl_str_mv	Rouws, Luc Felicianus Marie
dc.contributor.advisor-co1ID.fl_str_mv	014.563.806-56
dc.contributor.advisor-co1Lattes.fl_str_mv	http://lattes.cnpq.br/4500797890789377
dc.contributor.referee1.fl_str_mv	Zilli, Jerri ?dson
dc.contributor.referee2.fl_str_mv	Medici, Leonardo Oliveira
dc.contributor.authorID.fl_str_mv	602.783.683-01
dc.contributor.authorLattes.fl_str_mv	http://lattes.cnpq.br/0129530048505952
dc.contributor.author.fl_str_mv	Matos, Gustavo Feitosa de
contributor_str_mv	Baldani, Jos? Ivo Rouws, Luc Felicianus Marie Zilli, Jerri ?dson Medici, Leonardo Oliveira
dc.subject.por.fl_str_mv	Bradyrhizobium taxonomia de procariotos promo??o de crescimento em cana-de-a??car
topic	Bradyrhizobium taxonomia de procariotos promo??o de crescimento em cana-de-a??car Bradyrhizobium taxonomy of prokaryotes Promotion of growth in sugarcane Agronomia
dc.subject.eng.fl_str_mv	Bradyrhizobium taxonomy of prokaryotes Promotion of growth in sugarcane
dc.subject.cnpq.fl_str_mv	Agronomia
description	Recent molecular biology studies have indicated that bacteria of the genus Bradyrhizobium and Rhizobium may also play a role in the biological nitrogen fixation process (BNF) when associated with sugarcane. The use of Vigna unguiculata trap plants allowed obtaining a diverse collection of endophytic Bradyrhizobium spp. from sugarcane roots. The present work aimed to characterize a group of bacteria of the genus Bradyrhizobium from sugarcane roots, as well as to evaluate the ability of a representative strain of this group and an isolate of the genus Rhizobium to promote growth in this crop. To define the taxonomic position of the isolates of the group, phylogenetic analyzes were performed with the individual 16S rRNA, ITS, nodC and recA genes and multilocus sequence analysis (MLSA) involving four housekeeping genes (recA, dnaK, glnII and atpD), in four representative isolates (BR 10280T, BR 10266, BR 10555 and BR 10556). In addition, biochemical and morphophysiological tests were performed. Experiments in which the isolates P9-20 (BR 10280) (Bradyrhizobium sp.) and P5-2 (Rhizobium sp.) were inoculated in sugarcane seedlings were conducted in a greenhouse to evaluate the growth promoting effect of these isolates. Two harvests were performed at 30 and 75 days after transplanting (DAT). Among the analyzed variables were the budding speed index (BSI), dry mass and total nitrogen (N). Phylogenetic analyzes positioned the isolates in the superclade of B. japonicum in an independent branch close to B. huanghuaihaiense, a soybean micro-symbiont. Despite the proximity of the group under study to B. huanghuaihaiense, these isolates did not induce nodulation in Glycine max. In addition, unlike B. huanghuaihaiense, the new isolates induced nodule formation in Phaseolus vulgaris. In morphophysiological studies, significant differences were demonstrated between the representative isolates from this study and B. huanghuaihaiense. In the greenhouse experiment, an increase on BSI and root dry mass of the inoculated treatment with the P5-2 isolate was observed in the first harvest. In the second harvest, increments were found in the root dry mass and root volume, as well as in the total N of the roots and aerial part, in the treatment inoculated with P5-2, although no statistical difference was detected. These results of the characterization indicate that the sugarcane isolates of the genus Bradyrhizobium represent a new species of this genus. In relation to the greenhouse experiment, the isolate of the genus Rhizobium sp. presented potential as a growth promoter in the sugarcane crop.
publishDate	2017
dc.date.issued.fl_str_mv	2017-02-23
dc.date.accessioned.fl_str_mv	2018-09-13T17:03:38Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/masterThesis
format	masterThesis
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	MATOS, Gustavo Feitosa de. Caracteriza??o de novos riz?bios isolados de ra?zes de cana-de-a??car. 2017. 57 f. Disserta??o (Mestrado em Fitotecnia) - Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2017.
dc.identifier.uri.fl_str_mv	https://tede.ufrrj.br/jspui/handle/jspui/2436
identifier_str_mv	MATOS, Gustavo Feitosa de. Caracteriza??o de novos riz?bios isolados de ra?zes de cana-de-a??car. 2017. 57 f. Disserta??o (Mestrado em Fitotecnia) - Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2017.
url	https://tede.ufrrj.br/jspui/handle/jspui/2436
dc.language.iso.fl_str_mv	por
language	por
dc.relation.references.por.fl_str_mv	ALMEIDA, N. F.; YAN, S. C.; CAI, R. M.; CLARKE, C. R.; MORRIS, C. E.; SCHAAD, N. W.; SCHUENZEL, E. L.; LACY, G. H.; SUN, X. A., JONES, J. B.; CASTILLO, J. A.; BULL, C. T.; LEMAN, S.; GUTTMAN, D. S.; SETUBAL, J. C.; VINATZER, B. A. A multilocus sequence typing and analysis database and website for plant-associated Microbes. Phytopathology, v. 100, n. 3, p. 208-215, 2010. ALTSCHUL, S.F.; MADDEN, T.L.; SCH?FFER, A.A.; ZHANG, J.; ZHANG, Z.; MILLER, W.; AND LIPMAN, D.J. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res, v. 25, n. 17, p. 3389-3402, 1997. AMANN, R. AND W. LUDWIG. Ribossoma RNA-targeted nucleic acid probes for studies in microbial ecology. FEMS Microbiology Reviews, v. 24, n. 5, p. 555-565, 2000. ANDO, S.; GOTO, M.; MEUNCHANG S.; THONGRA-AR, P.; FUJIWARA, T.; HAYASHI, H.; YONEYAMA, T. Detection of nifH sequences in sugarcane (Saccharum officinarum L.) and pineapple (Ananas comosus [L.] Merr.). Soil Science & Plant Nutrition, v. 51, n. 2, p. 303-308, 2005. ARAUJO, E.O.; ROCHA, J. R.; GEROLA, J. G.; MATTE, L. C. Diazotrophic bacteria inoculation associates with acids and nitrogen in corn. African Journal of Plant Science, v. 10, n. 8, p. 162-166, 2016. BALDANI, J.I.; BALDANI, V.; SELDIN, L.; D?BEREINER, J. Characterization of Herbaspirillum seropedicae gen. nov., sp. nov., a root-associated nitrogen-fixing bacterium. International Journal of Systematic and Evolutionary Microbiology, v. 36, p. 86-93, 1986. BALDANI, J.I.; BALDANI, V.L.D.; SELDIN, L.; DOBEREINER, J. Characterization of Herbaspirillum seropedicae gen.nov., a root associated nitrogen fixing bacterium. International Journal of Systematic and Evolutionary Microbiology, v. 36, n. 1, p. 86-93, 1986. BALDANI, J.I.; CARUSO, L.; BALDANI, V.L.D.; GOI, S.R.; D?BEREINER, J. Recent advances in BNF with non-legume plants. Soil Biology and Biochemistry, v. 29, p. 911- 922, 1997. BALDANI, J.I.; POT, B.; KIRCHHOF, G.; FALSEN, E.; BALDANI, V.L.D.; OLIVARES, F.L.; HASTE, B.; KERSTERS, K.; HORTMANN, A.; GILLIS, M.; DOBEREINER, J. Emended description of Herbaspirillum, inclusion of (Pseudomonas) rubrisubalbicans, a mild plant pathogen, as Herbaspirillum rubrisubalbicans comb. nov.; and classification of a group of clinical isolates (EF group 1) as Herbaspirillum species 3. International Journal of Systematic and Evolutionary Microbiology, v. 46, n. 3, p. 802-810, 1996. BALDANI, J.I.; REIS, V.M.; VIDEIRA, S.S.; BODDEY, L.H.; BALDANI, V.L.D. The art of isolating nitrogen-fixing bacteria from non-leguminous plants using N-free semi-solid media: a practical guide for microbiologists. Plant and Soil, v. 384, p. 413-431, 2014. 40 BALDANI, V.L.D.; BALDANI, J.I.; D?BEREINER, J. Inoculation of rice plants with the endophytic diazotrophs Herbaspirillum seropedicae and Burkholderia spp.. Biology and Fertility of Soils, v. 30, n. 5, p. 485-491, 2000. BEIJERINCK, M.W. Cultur des Bacillus radicola aus den Kno llchen. Bot. Ztg., v. 46, p. 740?750, 1888. BENEDUZI, A.; MOREIRA, F.; COSTA, P.B.; VARGAS, L.K.; LISBOA, B.B.; FAVRETO, R. Diversity and plant growth promoting evaluation abilities of bacteria isolated from sugarcane cultivated in the South of Brazil. Applied Soil Ecology, v.63, p. 94?104, 2013. BHATTACHARJEE, R.B.; AQBAL, S.; MUKHOPADHYAY,S.N. Use of nitrogen?fixing bacteria as biofertiliser for non?legumes: prospects and challenges. Applied Microbiology and Biotechnology, v.80, p.199?209, 2008. BHATTACHARYYA, P.N.; JHA, D.K. Plant growth-promoting rhizobacteria (PGPR): emergence in agriculture. World Journal of Microbiology and Biotechnology, v. 28, p. 1327?1350, 2012. BOMAR, L.; MALTZ, M.; COLSTON, S.; GRAF, J. Direct culturing of microorganisms using metatranscriptomics. MBio, v. 2, n. 2, p. e00012-11, 2011. BROUGHTON, W.J.; HANIN, M.; RELIC, B.; KOPCINSKA, J.; GOLINOWSKI, W.; SIMSEK, S.; OJANEN-REUHS, T.; REUHS, B.; MARIE, C.; KOBAYASHI, H.; BORDOGNA, B.; LE QUERE, A.; JABBOURI, S.; FELLAY, R.; PERRET, X.; DEAKIN, W.J. Flavonoid-inducible modifications to rhamnan O antigens are necessary for Rhizobium sp. Strain NGR234- Legume Symbioses. Journal of Bacteriology, v. 188, n. 10, p. 3654-3663, 2006. BURBANO, C.S.; LIU, Y.; R?SNER, K.L.; REIS, V.M.; CABALLERO-MELLADO, J.; REINHOLD-HUREK, B.; HUREK T. Predominant nifH transcript phylotypes related to Rhizobium rosettiformans in field-grown sugarcane plants and in Norway spruce. Environmental microbiology reports, v. 3, p. 383-389, 2011. CARDINALE, M.; BRUSETTI, L.; QUATRINI, P. Comparison of different primer sets for use in automated ribosomal intergenic spacer analysis of complex bacterial communities. Applied Microbiology and Biotechnology, v. 70, p. 6147?56, 2004. CAVALCANTE, V.A.; DOBEREINER, J.A. New acid-tolerant nitrogenfixing bacterium associated with sugar-cane. Plant and Soil, v. 108, p. 23-31, 1988. CHAHBOUNE, R.; CARRO, L.; PEIX, A.; RAM?REZ-BAHENA, M.H.; BARRIJAL, S.; VEL?ZQUEZ, E.; BEDMAR, E. J. Bradyrhizobium rifense sp. nov. isolated from effective nodules of Cytisus villosus grown in the Moroccan Rif. Systematic and Applied Microbiology, v.35, p.302-305, 2012. CHAINTREUIL, C.; GIRAUD, E.; PRIN, Y.; LORQUIN, J.; B?, A.; GILLIS, M.; LAJUDIE, P.; DREYFUS, B. Photosynthetic Bradyrhizobia are natural endophytes of the African wild rice Oryza breviligulata. Applied and Environmental Microbiology, v. 66, p. 5437?5447, 2000. 41 CHAVES, V.; SANTOS, S.; SCHULTZ, N.; PEREIRA, W.; SOUSA, J.; MONTEIRO, R.; REIS, V. Desenvolvimento Inicial de Duas Variedades de Cana-de-a??car Inoculadas com Bact?rias Diazotr?ficas. Revista Brasileira Ci?ncia do Solo, Vi?osa-MG, v. 39, n. 6, p. 1595-1602, 2015. CHRISTENSEN, H.; KUHNERT, P.; BUSSE, H-J.; FREDERIKSEN, W.C.; BISGAARD,M. Proposed minimal standards for the description of genera, species and subspecies of the Pasteurellaceae. International Journal of Systematic Bacteriology, v. 57, p. 166-178, 2007. COUTINHO, H.L.C.; OLIVEIRA, V.M.; MANFIO, G.P.; ROSADO, A.S. Evoluating the microbial diversity of soil samples: methodological innovations. Anais da Academia de Ci?ncia, v.71, p.491-503, 1999. DALL?AGNOL, R.F.; RIBEIRO, R.A.; DELAMUTA, J.R.M.; ORMENO-ORRILLO, E.; ROGEL, M.A.; ANDRADE, D.S.; MART?NEZ-ROMERO, E.; HUNGRIA, M. Rhizobium paranaense sp. nov., an effective N2-fixing symbiont of common bean (Phaseolus vulgaris L.) with broad geographical distribution in Brazil. International Journal of Systematic and Evolutionary Microbiology, v. 64, p. 3222-3229, 2014. DALL?AGNOL, R.F.; DELAMUTA, J.R.M.; RIBEIRO, A.R.; HUNGRIA, M. Diversidade e filogenia de estirpes de riz?bios pela metedologia de MLSA. Embrapa Soja-Artigo em anais de congresso. responsabilidade socioambiental da pesquisa agr?cola: anais. Vi?osa: SBCS. 2014.4. Trab.1212, 2012. DAVIS, K.E.R.; SANGWAN, P.; JANSSEN, P.H. Acidobateria, Rubrobacteridae and Chloroflexi are abundant among very slow-growing and mini-colony-forming soil bacteria. Environmental microbiology, v. 13, n. 3, p. 798-805, 2011. DELAMUTA, J.R.M.; RIBEIRO, R.A.; ORME?O-ORRILLO, E.; MELO, I.S.; MARTI?NEZ-ROMERO, E.; HUNGRIA, M. Polyphasic evidence supporting the reclassification of Bradyrhizobium japonicum group Ia strains as Bradyrhizobium diazoefficiens sp. nov. International Journal of Systematic and Evolutionary Microbiology, v. 63, p. 3342?3351, 2013. D?NARI?, J.; DEBELL?, F.; PROM?, J.C. Rhizobium lipochitooligosaccharide nodulation factors: signalling molecules mediating recognition and morphogenesis. Annual Review Biochemistry, Palo Alto, v. 65, p. 503- 535, 1996. DIXON, R. AND KAHN, D. Genetic regulation of biological nitrogen fixation. Nature Reviews Microbiology, v. 2, n. 8, p. 621-631, 2004. DOBBELAERE, S.; VANDERLEYDEN, J.; OKON, Y. Plant growth-promoting effects of diazotrophs in the rhizosphere. Critical Reviews in Plant Sciences, v. 22, p. 107?149, 2003. DOBEREINER J.; RUSCHEL A. P. Uma nova esp?cie de Beijerinckia. Revista de Biologia, v. 1, p. 261-272, 1958. D?BEREINER, J. Nitrogen-fixing bacteria of the genus Beijerinckia Derx in the rhizosphere of sugar cane. Plant and Soil, v. 15, p. 211, 1961. 42 D?BEREINER, J. Recent changes in concepts of plant bacteria interactions: Endophytic Nz fixing bacteria. Ci?ncia e Cultura, v. 44, p. 310-313, 1992. DOBEREINER, J.; BALDANI, J.I.; BALDANI, V.L.D. Como isolar e identificar bact?rias diazotr?ficas de plantas n?o leguminosas. Embrapa SPI, 1995. EDGAR, R.C. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic acids research, v. 32, p. 1792-1797, 2004. FELSENSTEIN, J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution, p. 783-791, 1985. FERRARA, F.I.; OLIVEIRA, Z.M.; GONZALES, H.H.S.; FLOH, E.I.S.; BARBOSA, H.R. Endophytic and rhizospheric enterobacteria isolated from sugar cane have different potentials for producing plant growthpromoting substances. Plant and Soil, v. 353, p. 409?417, 2012. FERREIRA, D.F. Sisvar: a Guide for its Bootstrap procedures in multiple comparisons. Ci?ncia e Agrotecnologia, v. 38, n. 2, p. 109-112, 2014. FISCHER, D.; PFITZNER, B.; SCHMID, M. et al. Molecular characterization of the diazotrophic bacterial community in uninoculated and inoculated field-grown sugarcane (Saccharum sp.). Plant and soil, v. 356, n. 1-2, p. 83-99, 2012. FRANK, B. Ueber die Pilzsymbiose der Leguminosen. Bulletin der Akademie der Wissenschaft in Erakau, 1889. FURUSHITA, M.; SHIBA, T.; MAEDA, T.; YAHATA, M.; KANEOKA, A.; TAKAHASHI, Y.; TRII, K.; HASEGAWA, T.; OHTA, M. Similarity of tetracycline resistance genes isolated from fish farm bacteria to those from clinical isolates. Applied and Environmental Microbiology, v. 69, p. 5336?5342, 2003. GALTIER, N.; GOUY, M.; GAUTIER, C. SEAVIEW and PHYLO_WIN: two graphic tools for sequence alignment and molecular phylogeny. Computer applications in the biosciences: CABIOS, v. 12, n. 6, p. 543-548, 1996. GARC A-MART NE , J.; ACINA , .G.; RODR GUE -VALERA, F. Use of the 16S?23S ribosomal genes spacer region in studies of prokaryotic diversity. Journal of Microbiological Methods, v. 36, p. 55-64, 1999. GEVERS, D.; COHAN, F.M.; LAWRENCE, J.G.; SPRATT, B.G.; COENYE, T.; FEIL, E.J.; STACKEBRANDT, E.; de PEER, Y.V.; VANDAMME, P.; THOMPSON, F.L.; SWINGS, J. Re-evaluating prokaryotic species. Nature Reviews Microbiology, v.3, p.733-739, 2005. GILLIS, M.; KERSTIRS, K.; HOSTE, B.; JANSSENS, D.; KROPPENSTEDT, R.M.; STEPHOM, M.P.; TEXEIRA, K.R.S.; DOBEREINER, J.; LEY, J. Acetobacter diazotrophicus sp.nov., a nitrogen fixing acetic acid bacterium associated with sugar cane. International Journal of Systematic Bacteriology, v. 39, p.361-364, 1989. G?RIO, L.A.S.; DIAS, F.L.F.; REIS, V.M.; URQUIAGA, S.; SCHULTZ, N.; BOLONHEZI, D.; MUTTON, M.A. Bact?rias promotoras de crescimento e aduba??o nitrogenada no crescimento inicial de cana-de-a??car proveniente de mudas pr?-brotadas. Pesquisa Agropecu?ria Brasileira, v. 50, p. 33-43, 2015. 43 GLICK, B.R. The enhancement of plant growth by free-living bacteria. Canadian Journal of Microbiology, v. 41, p. 109?117, 1995. GOMES, A.A.; REIS, V.M.; BALDANI, V.L.D.; GOI, S.R. Rela??o entre a distribui??o de nitrog?nio e coloniza??o por bact?rias diazotr?ficas em cana de a??car. Pesquisa Agropecu?ria Brasileira, v. 40, p. 1105-1113, 2005. GOVINDARAJAN, M.; BALANDREAU, J.; MUTHUKUMARASAMY, R.; REVATHI, G.; LAKSHMINARASIMHAN, C. Improved yield of micropropagated sugarcane following inoculation by endophytic Burkholderia vietnamiensis. Plant end Soil, v. 280, p. 239?252, 2006. GOVINDARAJAN, M.; KWON, S-W.; WEON, H-Y. Isolation, molecular characterization and growth-promoting activities of endophytic sugarcane diazotroph Klebsiella sp.. World Journal Microbiol, v. 23, p. 997?1006, 2007. GUTI?RREZ?MA?ERO, F.J.; RAMOS?SOLANO, B.; PROBANZA, A.; MEHOUACHI, J.; TADEO, F.; TALON, M. The plant?growth?promoting rhizobacteria Bacillus pumilus and Bacillus licheniformis produce high amounts of physiologically active gibberellins. Physiologia Plantarum, v. 111, p. 206-211, 2001. HALLMANN, J.; QUADT-HALLMANN, A.; MAHAFFEE, W. F.; KLOEPPER, J. W. Bacterial endophytes in agricultural crops. Canadian Journal of Microbiology. V. 43, p. 895-914, 1997. HARDOIM, P.R.; VAN OVERBEEK, L.S.; BERG, G.; PIRTTIL?, A.M.; COMPANT, S.; CAMPISANO, A.; DORING, M.; SESSITSCH, A. The hidden world within plants: ecological and evolutionary considerations for defining functioning of microbial endophytes. Microbiology and Molecular Biology Reviews, v. 79, p. 293-320, 2015. JORDAN, D.C. NOTES: transfer of Rhizobium japonicum Buchanan 1980 to Bradyrhizobium gen. nov., a genus of slow-growing, root nodule bacteria from leguminous plants. International Journal of Systematic and Evolutionary Microbiology, v. 32, n. 1, p. 136-139, 1982. KAMST, E.; ESPANHAK, H.P.; KAFETZOPOULOS, D. Biosynthesis and secretion of rhizobial lipochitin-oligosaccharide signal molecules. Subcellular Biochemistry, v. 29, p. 29-71, 1998. KAMST, E.; PILLING, J.; RAAMSDONK, L.M.; LUGTENBERG, B.J.J.; ESPANHAK, H.P. Rhizobium nodulation protein NodC is an important determinant of chitin oligosaccharide chain length in Nod factor biosynthesis, v.179, p.2103-2108, 1997. KANEKO, T.; NAKAMURA, Y.; SATO, S.; MINAMISAWA, K.; UCHIUMI, T.; SASAMOTO, S.; WATANABE, A.; IDESAWA, K.; IRIGUCHI, M.; KAWASHIMA, K.; KOHARA, M.; MATSUMOTO, M.; SHIMPO, S.; TSURUOKA, H.; WADA, T.; YAMADA, M.; TABATA, S. Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110. DNA Research, v. 9, p. 189-197, 2002. 44 KENNEDY, I.; CHOUDHURY, A.; KECSK?S, M. Non-symbiotic bacterial diazotrophs in crop-farming systems: can their potential for plant growth promotion be better exploited? Soil Biology and Biochemistry, v. 36, p. 1229?1244, 2004. KONSTANTINIDIS K.T.; TIEDJE J.M. Prokaryotic taxonomy and phylogeny in the genomic era: advancements and challenges ahead. Current opinion in microbiology, v. 10, p. 504-509, 2007. KONSTANTINIDIS, K.T.; RAMETTE, A.; TIEDJE, J.M. Toward a more robust assessment of intraspecies diversity, using fewer genetic markers. Applied and Environmental Microbiology, v. 72, p. 7286?7293, 2006. KUYKENDALL, L.D.; SAXENA, B.; DEVINE, T.E.; UDELL, S.E. Genetic diversity in Bradyrhizobium japonicum (Jordan 1982) and a proposal for Bradyrhizobium elkanii sp. nov. Canadian Journal of Microbiology, v. 38, n. 6, p. 501-505, 1992. LAGUERRE, G.; NOUR, S.M.; MACHERET, V.; SANJUAN, J.; DROUIN, P.; AMARGER, N. Classification of rhizobia based on nodC and nifH gene analysis reveals a close phylogenetic relationship among Phaseolus vulgaris symbionts. Microbiology, v. 147, p. 981?993, 2001. LANDELL, M.G.A.; CAMPANA, M.P., FIGUEIREDO, P.; XAVIER, M.A.; ANJOS, I.A.; DINARDO-MIRANDA, L.L.; SCARPARI, M.S.; GARCIA, J.C.; BID?IA, M.A.P.; SILVA, D.N.; MENDON?A, J,R,; KANTHACK, R.A.D.; CAMPOS, M.F.; BRANCALI?O, S.R.; PETRI, R.H.; MIGUEL, P.E.M. Sistema de multiplica??o de cana-de-a??car com uso de mudas pr?-brotadas (MPB), oriundas de gemas individualizadas. Ribeir?o Preto: Instituto Agron?mico de Campinas, 2012. LAWSON, D.M. & SMITH, B.E. Molybdenum Nitrogenases. In: Metals Ions in Biological System: Volume 39: Molybdenum and Tungsten: Their Roles in Biological Processes:. CRC Press, 2002. LEDGARD, S.F. & STEELE, K.W. Biological nitrogen fixation in mixed legume/grass pastures. Plant end Soil, v. 141, p. 137, 1992. LIMA, E.; BODDEY, R.M.; D?BEREINER, J. Quantification of biological nitrogen fixation associated with sugarcane using a 15N aided nitrogen balance. Soil Biology and Biochemistry, v. 19, n. 2, p. 165-170, 1987. LUDWIG, W.; STRUNK, O.; WESTRAM, R.; RICHTER, L.; MEIER, H.; YADHUKUMAR, A.; et al. ARB: a software environment for sequence data. Nucleic acids research, v. 32, n. 4, p. 1363-1371, 2004. MAGALH?ES, F.M.; BALDANI, J.I.; SOUTO, S.M.; KUYKENDALL, J.R.; D?BEREINER, J. A new acid-tolerant Azospirillum species. Anais-Academia Brasileira de Ciencias, 1983. MAGNANI, G.S.; DIDONET, C.M.; CRUZ, L.M.; PICHETH, C.F.; PEDROSA, F.O.; SOUZA, E.M. Diversity of endophytic bacteria in Brazilian sugarcane. Genetics and Molecular Research, v. 9, p. 250?258, 2010. 45 MAGUIRE, J.D. Speed of germination aid in selection and evaluation for seedling emergence and vigor. Crop Sci.;2:176-7, 1962. MARCELLETTI, S.; FERRANTE, P.; SCORTICHINI, M. Multilocus sequence typing reveals relevant genetic variation and different evolutionary dynamics among strains of Xanthomonas arboricola pv. juglandis. Diversity, v. 2, n. 11, p. 1205-1222, 2010. MARQUES J?NIOR, R.B.; CANELLAS, L.P.; SILVA, L.G.; OLIVARES, F.P. Promo??o de enraizamento de microtoletes de cana-de-a??car pelo uso conjunto de subst?ncias h?micas e bact?rias diazotr?ficas endof?ticas. Revista Brasileira de Ci?ncia do Solo, v. 32, p. 1121-1128, 2008. MATOS, G. F.; COELHO, M.R.R., BALDANI, J.I.; REIS, V.M.; URQUIAGA, S.; ROUWS, L.F.M. Positive effects os endophytic (Brady)rhizobium isolates on sugarcane sett germination and root growth under glass house conditions. In: 10th International PGPR WorKshop, Li?ge. From Omics to the Field, 2015. MATOS, G.F. Isolamento, caracteriza??o e aplica??o de riz?bios naturalmente associadas ? cana-de-a??car, usando novas estrat?gias de cultivo. Monografia do curso de gradua??o em Agronomia. Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, RJ, 39 p. 2015. MATTHEWS, S.S; SPARKES, D.L.; BULLARD, M.J. The response of wheat to inoculation with the diazotroph Azorhizobium caulinodans. Aspects Appl Biol, v. 63, p. 35?42, 2001. MENNA, P.; BARCELLOS, F.G.; HUNGRIA, M. Phylogeny and taxonomy of a diverse collection of Bradyrhizobium strains based on Multilocus Sequence Analysis of the 16S rRNA gene, ITS region and gln II, rec A, ATP D and dnaK genes. International Journal of Systematic and Evolutionary Microbiology, v. 59, p. 2934-2950. 2009. MOREIRA, F.M.S.; SILVA, K.; N?BREGA, R.S.A.; CARVALHO, F. Bact?rias diazotr?ficas associativas: diversidade ecologia e potencial de aplica??es. Comunicata Scientiae, v. 1, p. 74 ? 99, 2010. MOREIRA, F.M.S.; SIQUEIRA, J. O. Microbiologia e bioqu?mica do solo. 2. ed. Lavras: UFLA, p. 729, 2006. MULLIS, K.; FALOONA, F.; SCHARF, S.; SAIKI, R.; HORN, G.; ERLICH, H. Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. Cold Spring Harbor Symposia on Quantitative Biology, v. 51, p. 263-273, 1986. NAGPAL, M.L.; FOX, K.F.; FOX A. Utility of 16S?23S rRNA spacer region methodology: how similar are interspace regions within a genome and between strains for closely related organisms? Journal of Microbiological Methods, v. 33, 211?219, 1998. NOGUEIRA, A.R.A.; SOUZA, G.B. Manual de laborat?rio: solo, ?gua, nutri??o vegetal, nutri??o animal e alimentos. Embrapa Pecu?ria Sudeste, p. 313, 2005. NORRIS, D.O.; DATE, R.A. Legume bacteriology. Tropical Pastures Research; Principles and Methods. p, p. 134-174, 1976. 46 OLDROYD G.; SPEAK, F.; ENTER. Signalling systems that promote beneficial symbiotic associations in plants. Nature Reviews Microbiology, v. 11, p. 252?263, 2013. OLDROYD, G.E.; MURRAY, J.D.; POOLE P.S.; DOWNIE, J.A. The rules of engagement in the legume-rhizobial symbiosis. Annual Review of Genetics, v. 45, p. 119-44, 2011. OLIVARES, F.L.; JAMES, E.K.; BALDANI, J.I.; DOBEREINER, J. Infection of mottle strip disease-susceptible and resistant sugar cane varieties by the endophytc diazotroph Herbaspirillum. New phytologist, Cambridge, Gr?-Bretanha, v.135, p.575-597, 1997. OLIVEIRA, A.L.M.; CANUTO, E.L.; REIS, V.M.; BALDANI, J.I. Response of micropropagated sugarcane varieties to inoculation with endophytic diazotrophic bacteria. Brazilian Journal of Microbiology, v. 34, p. 59-61, 2003. OLIVEIRA, A.L.M.; URQUIAGA, S.; D?BEREINER, J. & BALDANI, J.I. The effect of inoculating endophytic N2 fixing bacteria on micro propagated sugarcane plants. Plant and Soil, v. 242, p. 205-215, 2002. OLIVEIRA, R.P.; SCHULTZ, N.; MONTEIRO, R.C.; PEREIRA, W.; DE ARA?JO, A.P.; URQUIAGA, S.; REIS, V.M. Growth analysis of sugarcane inoculated with diazotrophic bacteria and nitrogen fertilization. African Journal of Agricultural Research, v. 11, p. 2786-2795, 2016. ORME?O-ORRILLO, E.; RIBEIRO, R.A.; HELENE, L.C.F.; HUNGRIA, M.; DELAMUTA, J.R.M.; MART?NEZ-ROMERO, E.; ROGEL, M.A. Bradyrhizobium viridifuturi sp. nov., encompassing nitrogen-fixing symbionts of legumes used for greenmanure and environmental services. International Journal of Systematic and Evolutionary Microbiology. v. 65, p. 4441-4448, 2015. PAUNGFOO-LONHIENNE, C.; LONHIENNE, T.G.A.; YEOH, Y.K.; WEBB, R.I.; LAKSHMANAN, P.; CHAN, C.X.; LIM, P.E.; RAGAN, M.A.; SCHMIDT, S.; HUGENHOLTZ, P. A new species of Burkholderia isolated from sugarcane roots promotes plant growth. Microbial Biotechnology, v. 7, p. 142?154, 2014. PIROMYOU, P.T.; GREETATORN, K.; TEAMTISONG, T.; OKUBO, R.; SHINODA, A.; NUNTAKIJ, P.; TITTABUTR, N.; BOONKERD, K.; MINAMISAWA, AND N. TEAUMROONG. Preferential Association of Endophytic Bradyrhizobia with Different Rice Cultivars and Its Implications for Rice Endophyte Evolution. Applied and environmental microbiology, v. 81, n. 9, p. 3049-3061, 2015. QUECINE, M.C.; ARA?JO, W.L.; ROSSETTO, P.B.; FERREIRA, A.; TSUI, S.; LACAVA, P.T.; PIZZIRANI-KLEINER, A.A. Sugarcane Growth Promotion by the Endophytic Bacterium Pantoea agglomerans 33.1. Applied and Environmental Microbiology, v. 78, p. 7511?7518, 2012. R CORE TEAM. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. URL https://www.R-project.org/, 2016. REES, D.C. & HOWARD, J.B. Nitrogenase: standing at the crossroads. Current opinion in chemical biology, v. 4, n. 5, p. 559-566, 2000. 47 REINHOLD-HUREK, B. & HUREK, T. Living inside plants: bacterial endophytes. Current opinion in plant biology, v. 14, n. 4, p. 435-443, 2011. REINHOLD-HUREK, B.; HUREK, T. Life in grasses: diazotrophic endophytes. Trends in Microbiology v. 6, p. 139?144, 1998. REIS JUNIOR, F.B.; MENDES, I.E.; TEIXEIRA, K.R.S.; REIS, V.M. Uso de Ferramentas Moleculares em Estudos da Diversidade de Microrganismos do Solo. Planaltina, DF: Embrapa Cerrados, 2002. REIS JUNIOR, F.B; REIS, V.M.; URQUIAGA, S.; D?BEREINER, J. Influence of nitrogen fertilisation on the population of diazotrophic bacteria Herbaspirillum spp. and Acetobacter diazotrophicus in sugar cane (Saccharum spp.). Plant and Soil, v.219, p.153-159, 2000. REIS, F.B., SILVA, L.G.; REIS, V.M.; DOBEREINER, J. Occurrence of diazotrophic bacteria in different sugar cane genotypes. Pesquisa Agropecuaria Brasileira v. 35, p. 985? 994, 2000. REIS, V.M.; BALDANI, J.I.; URQUIAGA, S. Recomenda??o de uma mistura de estirpes de cinco bact?rias fixadores de nitrog?nio para inocula??o de cana-de-a??car: Gluconacetobacter diazotrophicus (BR 11281), Herbaspirillum seropedicae (BR 11335), Herbaspirillum rubrisubalbicans (BR 11504), Azospirillum amazonense (BR 11145) e Burkholderia tropica (BR11366). Embrapa Agrobiologia. Circular T?cnica, 2009. RICHARDSON, A.E.; BAREA, J.M.; MCNEILL, A.M. et al. Acquisition of phodphorus and nitrogen in the rhizosphere and plant growth promocion by mricroorganisms. Plant and Soil, v. 321, p. 305, 2009. RICHTER, M.; ROSSELL?-M?RA, R. Shifting the genomic gold standard for the prokaryotic species definition. Proceedings of the National Academy of Sciences, v. 106, p. 19126-19131, 2009. RIVAS, R.; MARTENS, M.; LAJUDIE, P.; WILLEMS, A. Multilocus sequence analysis of the genus Bradyrhizobium. Systematic and applied microbiology, v. 32, p. 101-110, 2009. RIVAS, R.; MARTENS, M.; LAJUDIE, P.; WILLEMS, A. Multilocus sequence analysis of the genus Bradyrhizobium. Systematic and applied microbiology, v. 32, p. 101-110, 2009. RIVAS, R.; WILLEMS, A.; PALOMO, J.L.; GARC?A-BENAVIDES, P.; MATEOS, P.F.; MART?NEZ-MOLINA, E.; GILLIS, M.; VEL?ZQUEZ, E. Bradyrhizobium betae sp. nov., isolated from roots of Beta vulgaris affected by tumour-like deformations. International Journal of Systematic Bacteriology, v. 54, p. 1271?1275, 2004. ROBERTSON, G.P.; VITOUSEK, P.M. Nitrogen in agriculture: balancing the cost of an essential resource. Annual Review of Environment and Resources, v. 34, p. 97?125, 2009. RODRIGUEZ NETO, J.; MALAVOLTA JUNIOR, V.A.; VICTOR, O. Meio Simples para isolamento e cultivo de Xanthomonas campestris pv. Citri tipo B. Summa Phytopathol, v. 12, n. 1-2, p. 32, 1986. RODRIGUEZ, H.; FRAGA, R. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnology advances, v. 17, p. 319-339, 1999. 48 ROESCH, L.F.; SELBACH, P.A.; S?, E.S. Reinocula??o de bact?rias diazotr?ficas aumentando o crescimento de plantas de trigo. Ci?ncia Rural, Santa Maria, v. 35, n.5, p. 1201-1204, 2005. ROSADO, A.S.; DUARTE, G.F.; MENDON?A-HAGLER, L.C. A moderna microbiologia do solo: Aplica??o de t?cnicas de biologia molecular. In: SIQUEIRA, J.D.; MOREIRA, F.M.S.; LOPES, A.S.; GUILHERME, LR.G.; FAQUIM, V.; FRUTINI-NETO, A.E.; CARVALHO, J.G. (Ed.). Inter-rela??o fertilidade, biologia do solo e nutri??o de plantas. Vi?osa: SBCS, Lavras: UFLA/DSC. p. 429-448, 1999. ROSENBLUETH, M. and MARTINEZ-ROMERO, E. Bacterial endophytes and their interactions with hosts. Molecular plant-microbe interactions, v. 19, n. 8, p. 827-837, 2006. ROSSEL?-MORA, R.; AMANN, R. The species concept for prokaryotes. FEMS Microbiology Review, v. 25, n. 1, p. 39-67, 2001. ROUWS, L.F.M.; LEITE, J.; MATOS, G.F.; ZILLI, J.E.; COELHO, M. R.R.; XAVIER, G. R.; FISCHER, D.; HARTMANN, A.; REIS, V.M., BALDANI, J.I. Endophytic Bradyrhizobium spp. isolates from sugarcane obtained through different culture strategies. Environmental microbiology reports, v. 6, n. 4, p. 354-363, 2013. RUSCHEL, A.P.; HENIS, Y.; SALATI E. Nitrogen-15 tracing of N-fixation with soil-grown sugarcane seedlings. Soil Biology and Biochemistry, v. 7, n. 2, p. 181-182, 1975. SAITOU, N.; NEI, M. The Neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, v. 4, p. 406-425, 1987. SALEH, S.A.; MEKHEMAR G.A.A.; EL-SOUD, A.A.A.; RAGAB, A.A.; MIKHAEEL, F.T. Survival of Azorhizobium and Azospirillum in different carrier materials: inoculation of wheat and Sesbania rostrata. Bulletin of Faculty of Agriculture, v. 52, p. 319?338, 2001. SANTI, C.; BOGUSZ, D.; FRANCHE, C. Biological nitrogen fixation in nonlegume plants. Annals of Botany, v. 111, p. 743?767, 2013. SANTOYO, G.; MORENO-HAGELSIEB, G.; OROZCO-MOSQUEDA, M.C.; GLICK, B.R, Plant growth-promoting bacterial endophytes. Microbiological Research, v. 183, p. 92?99, 2016. SARITA, S.; SHARMA, P.K.; PRIEFER, U.B.; PRELL, J. Direct amplification of rhizobial nodC sequences from soiltotal DNA and comparison to nodC diversity of root nodule isolates. FEMS microbiology ecology, v. 54, n. 1, p. 1-11, 2005. SCHULTZ, N.; SILVA, J.A.; SOUSA, J.S.; MONTEIRO, R.C.; OLIVEIRA, R.P.; CHAVES, V.A.; PEREIRA, W.; SILVA, M.; BALDANI, J.I.; BODDEY, R.M.; REIS, V.M.; URQUIAGA, S. Inoculation of sugarcane with diazotrophic bacteria. Revista Brasileira de Ci?ncia do Solo, v. 38, p. 407-414, 2014. SILVA, C., VINUESA, P., EGUIARTE, L.E., SOUZA, V., MART?NEZ-ROMERO, E. Evolutionary genetics and biogeographic structure of Rhizobium gallicum sensu lato, a widely 49 distributed bacterial symbiont of diverse legumes. Molecular Ecology, v. 14, p. 4033-4050, 2005. SOUZA, R.S.C.; OKURA, V.K.; ARMANHI, J.S.L.; JORR,?I.N.B.; LOZANO, N.; SILVA, M.J.; GONZ?ALEZ-GUERRERO, M.; ARAUJO, L.M.; FERREIRA, N.V.; BAGHERI, H.C.; IMPERIAL, J.; ARRUDA, P. Unlocking the bacterial and fungal communities assemblages of sugarcane microbiome. Scientific Reports v. 6, p. 28774, 2016. STACKEBRANDT, E.; GOEBEL, B. Taxonomic note. A place for DNA?DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. International Journal of Systematic and Evolutionary Microbiology, v. 44, n. 4, p. 846-849, 1994. STEPKOWSKI, T.; CZAPLINSKA, M., MIEDZINSKA, K.; MOULIN, L. The variable part of the dnaK gene as an alternative marker for phylogenetic studies of rhizobia and related alpha Proteobacteria. Systematic and Applied Microbiology, v.26, p.483-494, 2003. STEPKOWSKI, T.; MOULIN, L.; KRZYZANSKA, A.; MCINNES, A.; LAW, I.J.; HOWIESON, J. European origin of Bradyrhizobium populations infecting lupins and serradella in soils of western Australia and South Africa. Applied and Environmental Microbiology, v. 71, n. 11, p. 7041-7052, 2005. STEVENSON, B.S.; EICHORST, S.A.; WERTZ, J.T.; SCHMIDT, T.M.; BREZNAK, J.A. New strategies for cultivation and detection of previously uncultered microbes. Applied and Environmental Microbiology. v. 70, p. 4748-4755, 2004. TAMURA, K.; STECHER, G.; PETERSON, D.; FILIPSKI, A.; KUMAR, S. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution v. 30, p. 2725-2729, 2013. TEALE, W.; PAPONOV, I.; PALME, K. Auxin in action: signalling, transport and the control of plant growth and development. Nature Reviews Molecular Cell Biology, v. 7, n. 11, p. 847-859, 2006. THAWEENUT, N.; HACHISUKA, Y.; ANDO, S.; YANAGISAWA, S.; YONEYAMA, T. Two seasons study of nifH gene expression and nitrogen fixation by diazotrophic endophytes in sugarcane (Saccharum spp. hybrids): expression of nifH genes similar to those of rhizobia. Plant and Soil, v. 338, p. 435-449, 2011. TIGHE, S.W.; DE LAJUDIE, P.; DIPIETRO, K.; LINDSTR?M, K.; NICK, G.; JARVIS, B. D. Analysis of cellular fatty acids and phenotypic relationships of Agrobacterium, Bradyrhizobium, Mesorhizobium, Rhizobium and Sinorhizobium species using the Sherlock Microbial Identification System. International journal of systematic and evolutionary microbiology, v. 50, p. 787-801, 2000. TILMAN, D.; CASSMAN, K.G.; MATSON, P.A.; NAYLOR, R.; POLASKY, S. Agricultural sustainability and intensive production practices. Nature, v. 418, n. 6898, p. 671-677, 2002. 50 TOLEDO, B.F.B. Identifica??o de estirpes de riz?bios por sequenciamento parcial dos genes 16S rDNA e nifH. Graduate Program in Agronomy ? Plant Breeding and Genetics, UNESP, Jaboticabal, Brazil, 2008. UEDA, T.; SUGA, Y.; YAHIRO, N.;
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.publisher.none.fl_str_mv	Universidade Federal Rural do Rio de Janeiro
dc.publisher.program.fl_str_mv	Programa de P?s-Gradua??o em Fitotecnia
dc.publisher.initials.fl_str_mv	UFRRJ
dc.publisher.country.fl_str_mv	Brasil
dc.publisher.department.fl_str_mv	Instituto de Agronomia
publisher.none.fl_str_mv	Universidade Federal Rural do Rio de Janeiro
dc.source.none.fl_str_mv	reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ instname:Universidade Federal Rural do Rio de Janeiro (UFRRJ) instacron:UFRRJ
instname_str	Universidade Federal Rural do Rio de Janeiro (UFRRJ)
instacron_str	UFRRJ
institution	UFRRJ
reponame_str	Biblioteca Digital de Teses e Dissertações da UFRRJ
collection	Biblioteca Digital de Teses e Dissertações da UFRRJ
bitstream.url.fl_str_mv	http://localhost:8080/tede/bitstream/jspui/2436/18/2017+-+Gustavo+Feitosa+de+Matos.pdf.jpg http://localhost:8080/tede/bitstream/jspui/2436/17/2017+-+Gustavo+Feitosa+de+Matos.pdf.txt http://localhost:8080/tede/bitstream/jspui/2436/2/2017+-+Gustavo+Feitosa+de+Matos.pdf http://localhost:8080/tede/bitstream/jspui/2436/1/license.txt
bitstream.checksum.fl_str_mv	cc73c4c239a4c332d642ba1e7c7a9fb2 f5370c55f75ad879dce8a077ef22063f 0ed6966082e21d15123a12cd0e5777ef 7b5ba3d2445355f386edab96125d42b7
bitstream.checksumAlgorithm.fl_str_mv	MD5 MD5 MD5 MD5
repository.name.fl_str_mv	Biblioteca Digital de Teses e Dissertações da UFRRJ - Universidade Federal Rural do Rio de Janeiro (UFRRJ)
repository.mail.fl_str_mv	bibliot@ufrrj.br\|\|bibliot@ufrrj.br
_version_	1800313482780868608

Caracteriza??o de novos riz?bios isolados de ra?zes de cana-de-a??car

Registros relacionados