Papel dos linf?citos B-1 na imunopatog?nese do L?pus eritematoso sist?mico

• Autor(a) principal: Silva, Amanda Couto
• Data de Publicação: 2021
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
• Texto Completo: https://tede.ufrrj.br/jspui/handle/jspui/5917
• topic: Linf?citos B-1; L?pus eritematoso sist?mico; Imunomodula??o; Autoimunidade; B-1 lymphocytes; Systemic lupus erythematosus; Immunomodulation; autoimmunity; Medicina Veterin?ria

Systemic lupus erythematosus (SLE) is a chronic autoimmune disease that affects dogs, cats, horses, humans and primates. The pathogenesis is multifactorial, being associated with genetic, hormonal and environmental factors. SLE leads to deposition of immune complexes in joints and various organs, resulting in clinical manifestations. The participation of T and B lymphocytes is crucial for the pathogenesis of the disease. B-1 lymphocytes, a subpopulation of B cells, have characteristics that may contribute to the pathogenesis of autoimmune diseases through the secretion of cytokines such as IL-10 exerting a modulating action on both the acute and chronic inflammatory response, presenting antigens to T cells, participating of innate and adaptive immunity and are the main producers of natural antibodies. Understanding the role of B-1 cells in SLE immunopathogenesis may open new fronts for studies on immunotherapeutic strategies to control this complex and multifactorial disease. The present work evaluated the role of B-1 cells in the immunopathogenesis of SLE using the pristane induction model. SLE was induced in BALB/c, XID (B-1 deficient) and XID mice repopulated with B-1. The animals were evaluated for six months. We found that BALB/c pristane and XID repopulated with B-1 showed characteristic signs of the disease, such as lipogranuloma formation and splenomegaly. Notably, BALB/c pristane also presented ascites, joint edema, arthritis, kidney damage with immune complex deposition. In the spleen, animals BALB/c had a higher percentage of B cells (CD19+, IgM+), BALB/c pristane T CD8 (CD3+, CD8+), while XID pristane had increased T CD4 (CD3+,CD4+). In the peritoneal lavage, after induction with pristane, there was a decrease in B cells (CD19+, IgM+) and an increase in B1a (CD19+, IgM CD5+) in BALB/c pristane. In peripheral blood, BALB/c had a higher number of lymphocytes, XID had a neutrophilic profile, and all groups with SLE showed an increase in monocytes. The cytokines IL-10, IL-6 and IFN-? were increased in BALB/c and XID repopulated that developed SLE. Based on these results, we suggest that the presence of B-1 cells may contribute to the development of SLE.