Características morfológicas, fisiológicas e transcriptoma em variedades de arroz (Oryza sativa L.) contrastantes quanto a tolerância ao estresse hídrico

Detalhes bibliográficos
Autor(a) principal: Ferreira, Leandro Martins
Data de Publicação: 2017
Tipo de documento: Tese
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
Texto Completo: https://rima.ufrrj.br/jspui/handle/20.500.14407/9057
Resumo: Dentre os estresses abióticos que podem limitar o crescimento das culturas agrícolas, a seca é considerada um dos principais, sendo capaz de reduzir consideravelmente a produção global de alimentos. O arroz é uma das mais importantes culturas agrícolas do mundo e sua produção demanda grande quantidade de água, pois é uma espécie extremamente sensível ao déficit hídrico. Portanto, a obtenção de plantas de arroz que lidam com o estresse hídrico, sem redução significativa de produtividade é um desafio para os programas de melhoramento atuais. Este trabalho teve como objetivos: (i) identificar variedades de arroz de sequeiro contrastantes quanto à tolerância ao estresse hídrico por meio da avaliação de características morfológicas e fisiológicas, (ii) analisar parâmetros radiculares que possam explicar a diferença entre variedades tolerantes e sensíveis ao estresse hídrico e, (iii) identificar novos alvos biotecnológicos envolvidos com essa tolerância por meio da análise do perfil de transcritos nas variedades de arroz contrastantes. Foram realizados seis experimentos, sendo dois em casa de vegetação e quatro em câmara de crescimento. O delineamento experimental utilizado foi o inteiramente casualizado. O primeiro experimento iniciou com dez variedades de arroz submetidas a condição controle e estresse hídrico durante o período reprodutivo. As variedades contrastantes foram selecionadas com base em características morfológicas e fisiológicas analisadas. Os experimentos II a IV foram realizados a fim de correlacionar a tolerância ao estresse com o desenvolvimento e morfologia do sistema radicular. O experimento V foi realizado para avaliar a regulação de genes relacionados a tolerância ao estresse hídrico e o experimento VI teve como objetivo analisar a expressão diferencial de genes por meio da técnica de RNA-seq em raízes de arroz. Os dados obtidos dos componentes de produtividade, índices de tolerância ao estresse e análise multivariada das características morfológicas e fisiológicas permitiram identificar as variedades Catetão e Piauí como as mais tolerantes ao estresse hídrico, e Quebra Cacho e Mira como as mais sensíveis. Foi observado que a tolerância ao estresse hídrico está correlacionada com o menor ângulo radicular, aumento da densidade e emissão de raízes laterais em condições de déficit hídrico na variedade Catetão. Além disso, essa variedade mostra indução rápida e elevados níveis de expressão de genes e fatores de transcrição relacionados à tolerância ao estresse hídrico em arroz. Por meio do sequenciamento do RNA foi possível identificar diversos genes com potencial para serem utilizados em programas de melhoramento visando o aumento da tolerância ao estresse hídrico em arroz.
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spelling Ferreira, Leandro MartinsSantos, Leandro Azevedo983.907.835-68Souza, Sonia Regina de003.927.487-03Santos, Leandro AzevedoSantos, André Marques dosRossiello, Roberto Oscar PereyraMartins, Gilberto SachettoCabral, Luiz Mors107.560.167-38http://lattes.cnpq.br/69975443282944552023-12-21T18:33:59Z2023-12-21T18:33:59Z2017-02-22FERREIRA, Leandro Martins. Características morfológicas, fisiológicas e transcriptoma em variedades de arroz (Oryza sativa L.) contrastantes quanto à tolerância ao estresse hídrico. 2017. 110 f. Tese (Doutorado em Agronomia - Ciência do Solo). Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ. 2017.https://rima.ufrrj.br/jspui/handle/20.500.14407/9057Dentre os estresses abióticos que podem limitar o crescimento das culturas agrícolas, a seca é considerada um dos principais, sendo capaz de reduzir consideravelmente a produção global de alimentos. O arroz é uma das mais importantes culturas agrícolas do mundo e sua produção demanda grande quantidade de água, pois é uma espécie extremamente sensível ao déficit hídrico. Portanto, a obtenção de plantas de arroz que lidam com o estresse hídrico, sem redução significativa de produtividade é um desafio para os programas de melhoramento atuais. Este trabalho teve como objetivos: (i) identificar variedades de arroz de sequeiro contrastantes quanto à tolerância ao estresse hídrico por meio da avaliação de características morfológicas e fisiológicas, (ii) analisar parâmetros radiculares que possam explicar a diferença entre variedades tolerantes e sensíveis ao estresse hídrico e, (iii) identificar novos alvos biotecnológicos envolvidos com essa tolerância por meio da análise do perfil de transcritos nas variedades de arroz contrastantes. Foram realizados seis experimentos, sendo dois em casa de vegetação e quatro em câmara de crescimento. O delineamento experimental utilizado foi o inteiramente casualizado. O primeiro experimento iniciou com dez variedades de arroz submetidas a condição controle e estresse hídrico durante o período reprodutivo. As variedades contrastantes foram selecionadas com base em características morfológicas e fisiológicas analisadas. Os experimentos II a IV foram realizados a fim de correlacionar a tolerância ao estresse com o desenvolvimento e morfologia do sistema radicular. O experimento V foi realizado para avaliar a regulação de genes relacionados a tolerância ao estresse hídrico e o experimento VI teve como objetivo analisar a expressão diferencial de genes por meio da técnica de RNA-seq em raízes de arroz. Os dados obtidos dos componentes de produtividade, índices de tolerância ao estresse e análise multivariada das características morfológicas e fisiológicas permitiram identificar as variedades Catetão e Piauí como as mais tolerantes ao estresse hídrico, e Quebra Cacho e Mira como as mais sensíveis. Foi observado que a tolerância ao estresse hídrico está correlacionada com o menor ângulo radicular, aumento da densidade e emissão de raízes laterais em condições de déficit hídrico na variedade Catetão. Além disso, essa variedade mostra indução rápida e elevados níveis de expressão de genes e fatores de transcrição relacionados à tolerância ao estresse hídrico em arroz. Por meio do sequenciamento do RNA foi possível identificar diversos genes com potencial para serem utilizados em programas de melhoramento visando o aumento da tolerância ao estresse hídrico em arroz.CAPESFaperjCNPqAmong the abiotic stress, drought is a major environmental stress seriously limiting plant growth and crop productivity. Rice is one of the most important staple food crops in the world and requires a larger quantity of water to produce, once it is a crop extremely sensitive to drought stress. For this reason, to obtain rice plants that cope with drought stress without major reduction in productivity is the challenge for breeding programs nowadays. This work aimed: (i) identify upland rice varieties with contrasting drought tolerance through the evaluation of morphological and physiological traits, (ii) analyse root parameters which could explain the differences between tolerant and sensitive varieties to the drought stress and, (iii) identify new biotechnological targets related with the tolerance through transcript profile analysis in the contrasting varieties. Six experiments were performed, two in greenhouse and four in growth chamber conditions. The experimental design adopted was completely randomized. The first experiment started with ten rice varieties submitted to control and stress conditions during the reproductive stage. The contrasting varieties were selected based on morphological and physiological traits. Experiments from II to IV aimed to correlate the tolerance to the drought stress with the root development and morphology. Experiment V aimed to evaluate the regulation of genes related to the drought tolerance and the experiment VI aimed to analyse the differential expression of genes through the RNAseq analysis in rice roots. Data obtained from the productivity components, tolerance index and multivariate analysis through the evaluation of morphological and physiological traits allowed to identify Catetão and Piauí variety as the most tolerant and Quebra Cacho and Mira as the most sensitive. Drought tolerance was correlated with a lower root angle and increase in the root density and emission of lateral roots by Catetão variety during drought stress. Moreover, Catetão variety has showed higher expression levels and early induction of genes and transcription factors related with drought tolerance. The RNAseq analysis allowed to identify several potential genes which can be used in future breeding programs aimimg the improvement of drought tolerance in rice.application/pdfporUniversidade Federal Rural do Rio de JaneiroPrograma de Pós-Graduação em Agronomia - Ciência do SoloUFRRJBrasilInstituto de AgronomiaDroughtHigh-throughput sequencingRNA-seqReal time PCRSecaSequenciamento de nova geraçãoRNA-seqPCR em tempo realAgronomiaCaracterísticas morfológicas, fisiológicas e transcriptoma em variedades de arroz (Oryza sativa L.) contrastantes quanto a tolerância ao estresse hídricoMorphological, physiological and trancriptome traits of rice varieties (Oryza sativa L.) contrasting to the drought toleranceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisABDI, N.; DARVISHZADEH, R.; MALEKI HATAMI, H. Effective selection criteria for screening drought tolerant recombinant inbred lines of sunflower. Genetika, v. 45, n. 1, p. 153-166, 2013. 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dc.title.por.fl_str_mv Características morfológicas, fisiológicas e transcriptoma em variedades de arroz (Oryza sativa L.) contrastantes quanto a tolerância ao estresse hídrico
dc.title.alternative.eng.fl_str_mv Morphological, physiological and trancriptome traits of rice varieties (Oryza sativa L.) contrasting to the drought tolerance
title Características morfológicas, fisiológicas e transcriptoma em variedades de arroz (Oryza sativa L.) contrastantes quanto a tolerância ao estresse hídrico
spellingShingle Características morfológicas, fisiológicas e transcriptoma em variedades de arroz (Oryza sativa L.) contrastantes quanto a tolerância ao estresse hídrico
Ferreira, Leandro Martins
Drought
High-throughput sequencing
RNA-seq
Real time PCR
Seca
Sequenciamento de nova geração
RNA-seq
PCR em tempo real
Agronomia
title_short Características morfológicas, fisiológicas e transcriptoma em variedades de arroz (Oryza sativa L.) contrastantes quanto a tolerância ao estresse hídrico
title_full Características morfológicas, fisiológicas e transcriptoma em variedades de arroz (Oryza sativa L.) contrastantes quanto a tolerância ao estresse hídrico
title_fullStr Características morfológicas, fisiológicas e transcriptoma em variedades de arroz (Oryza sativa L.) contrastantes quanto a tolerância ao estresse hídrico
title_full_unstemmed Características morfológicas, fisiológicas e transcriptoma em variedades de arroz (Oryza sativa L.) contrastantes quanto a tolerância ao estresse hídrico
title_sort Características morfológicas, fisiológicas e transcriptoma em variedades de arroz (Oryza sativa L.) contrastantes quanto a tolerância ao estresse hídrico
author Ferreira, Leandro Martins
author_facet Ferreira, Leandro Martins
author_role author
dc.contributor.author.fl_str_mv Ferreira, Leandro Martins
dc.contributor.advisor1.fl_str_mv Santos, Leandro Azevedo
dc.contributor.advisor1ID.fl_str_mv 983.907.835-68
dc.contributor.advisor-co1.fl_str_mv Souza, Sonia Regina de
dc.contributor.advisor-co1ID.fl_str_mv 003.927.487-03
dc.contributor.referee1.fl_str_mv Santos, Leandro Azevedo
dc.contributor.referee2.fl_str_mv Santos, André Marques dos
dc.contributor.referee3.fl_str_mv Rossiello, Roberto Oscar Pereyra
dc.contributor.referee4.fl_str_mv Martins, Gilberto Sachetto
dc.contributor.referee5.fl_str_mv Cabral, Luiz Mors
dc.contributor.authorID.fl_str_mv 107.560.167-38
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/6997544328294455
contributor_str_mv Santos, Leandro Azevedo
Souza, Sonia Regina de
Santos, Leandro Azevedo
Santos, André Marques dos
Rossiello, Roberto Oscar Pereyra
Martins, Gilberto Sachetto
Cabral, Luiz Mors
dc.subject.eng.fl_str_mv Drought
High-throughput sequencing
RNA-seq
Real time PCR
topic Drought
High-throughput sequencing
RNA-seq
Real time PCR
Seca
Sequenciamento de nova geração
RNA-seq
PCR em tempo real
Agronomia
dc.subject.por.fl_str_mv Seca
Sequenciamento de nova geração
RNA-seq
PCR em tempo real
dc.subject.cnpq.fl_str_mv Agronomia
description Dentre os estresses abióticos que podem limitar o crescimento das culturas agrícolas, a seca é considerada um dos principais, sendo capaz de reduzir consideravelmente a produção global de alimentos. O arroz é uma das mais importantes culturas agrícolas do mundo e sua produção demanda grande quantidade de água, pois é uma espécie extremamente sensível ao déficit hídrico. Portanto, a obtenção de plantas de arroz que lidam com o estresse hídrico, sem redução significativa de produtividade é um desafio para os programas de melhoramento atuais. Este trabalho teve como objetivos: (i) identificar variedades de arroz de sequeiro contrastantes quanto à tolerância ao estresse hídrico por meio da avaliação de características morfológicas e fisiológicas, (ii) analisar parâmetros radiculares que possam explicar a diferença entre variedades tolerantes e sensíveis ao estresse hídrico e, (iii) identificar novos alvos biotecnológicos envolvidos com essa tolerância por meio da análise do perfil de transcritos nas variedades de arroz contrastantes. Foram realizados seis experimentos, sendo dois em casa de vegetação e quatro em câmara de crescimento. O delineamento experimental utilizado foi o inteiramente casualizado. O primeiro experimento iniciou com dez variedades de arroz submetidas a condição controle e estresse hídrico durante o período reprodutivo. As variedades contrastantes foram selecionadas com base em características morfológicas e fisiológicas analisadas. Os experimentos II a IV foram realizados a fim de correlacionar a tolerância ao estresse com o desenvolvimento e morfologia do sistema radicular. O experimento V foi realizado para avaliar a regulação de genes relacionados a tolerância ao estresse hídrico e o experimento VI teve como objetivo analisar a expressão diferencial de genes por meio da técnica de RNA-seq em raízes de arroz. Os dados obtidos dos componentes de produtividade, índices de tolerância ao estresse e análise multivariada das características morfológicas e fisiológicas permitiram identificar as variedades Catetão e Piauí como as mais tolerantes ao estresse hídrico, e Quebra Cacho e Mira como as mais sensíveis. Foi observado que a tolerância ao estresse hídrico está correlacionada com o menor ângulo radicular, aumento da densidade e emissão de raízes laterais em condições de déficit hídrico na variedade Catetão. Além disso, essa variedade mostra indução rápida e elevados níveis de expressão de genes e fatores de transcrição relacionados à tolerância ao estresse hídrico em arroz. Por meio do sequenciamento do RNA foi possível identificar diversos genes com potencial para serem utilizados em programas de melhoramento visando o aumento da tolerância ao estresse hídrico em arroz.
publishDate 2017
dc.date.issued.fl_str_mv 2017-02-22
dc.date.accessioned.fl_str_mv 2023-12-21T18:33:59Z
dc.date.available.fl_str_mv 2023-12-21T18:33:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv FERREIRA, Leandro Martins. Características morfológicas, fisiológicas e transcriptoma em variedades de arroz (Oryza sativa L.) contrastantes quanto à tolerância ao estresse hídrico. 2017. 110 f. Tese (Doutorado em Agronomia - Ciência do Solo). Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ. 2017.
dc.identifier.uri.fl_str_mv https://rima.ufrrj.br/jspui/handle/20.500.14407/9057
identifier_str_mv FERREIRA, Leandro Martins. Características morfológicas, fisiológicas e transcriptoma em variedades de arroz (Oryza sativa L.) contrastantes quanto à tolerância ao estresse hídrico. 2017. 110 f. Tese (Doutorado em Agronomia - Ciência do Solo). Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ. 2017.
url https://rima.ufrrj.br/jspui/handle/20.500.14407/9057
dc.language.iso.fl_str_mv por
language por
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