Caracteriza??o de Staphylococcus do grupo sciuri e analise fenogenot?pica da resist?ncia ? oxacilina em isolados de roedores e equinos do Instituto Biol?gico do Ex?rcito

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Autor(a) principal: Silva, Amanda Couto Calazans
Data de Publicação: 2013
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
Texto Completo: https://tede.ufrrj.br/jspui/handle/jspui/3443
Resumo: Oxacillin/methicillin-resistance in Staphylococcus is related to the integration of SSCmec, containing the mec complex, in the bacterial chromosome. The mecA gene codifies a variant of PBP2, termed PBP2a or PBP2?, whose affinity with the beta-lactamic antibiotics is very low. Complex mec contains mecA and its regulatory genes mecR1 and mecI. Its origin is still unknown, although evidences support that it is related to coagulase-negative staphylococci, once mecA and a homologue gene, pbpD, were both detected in Staphylococcus sciuri species group. The present work evaluated 210 samples of skin and ear swabs from rodents and 60 nasal swabs from equines of Army Biologic Institute, Rio de Janeiro. Pheno- and genotypic results were concordant for 59,52% (25/42) and 78,57% (11/14) S. lentus and S. sciuri isolates, respectively. The phenotypic antimicrobial resistance pattern was evaluated and correlated to the detection of mecA and pbpD genes. Also, mecA sequences from sciuri group strains were compared to others species in order to evaluate the degree of similarity among them. Although all S. sciuri isolates tested positive for pbpD, no correlation was observed with antimicrobial resistance for isolates that presented only mecA homologue On the other hand, isolates tested positive for mecA gene also presented phenotypic resistance in at least one assay. The alignment of the mecA gene showed that the nucleotide sequences were sorted into 2 different groups, one comprising the bovine strains and the other containing human and equine strains.
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spelling Souza, Miliane Moreira Soares de010.761.987-32http://lattes.cnpq.br/0865211214618618Coelho, Shana de Mattos de Oliveira106.751.817-71http://lattes.cnpq.br/2160878993935525Silva, Amanda Couto Calazans2020-04-20T18:42:49Z2013-03-15SILVA, Amanda Couto Calazans. Caracteriza??o de Staphylococcus do grupo sciuri e analise fenogenot?pica da resist?ncia ? oxacilina em isolados de roedores e equinos do Instituto Biol?gico do Ex?rcito. 2013. 42 f. Disserta??o (Mestrado em Ci?ncias Veterin?rias) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2013.https://tede.ufrrj.br/jspui/handle/jspui/3443Oxacillin/methicillin-resistance in Staphylococcus is related to the integration of SSCmec, containing the mec complex, in the bacterial chromosome. The mecA gene codifies a variant of PBP2, termed PBP2a or PBP2?, whose affinity with the beta-lactamic antibiotics is very low. Complex mec contains mecA and its regulatory genes mecR1 and mecI. Its origin is still unknown, although evidences support that it is related to coagulase-negative staphylococci, once mecA and a homologue gene, pbpD, were both detected in Staphylococcus sciuri species group. The present work evaluated 210 samples of skin and ear swabs from rodents and 60 nasal swabs from equines of Army Biologic Institute, Rio de Janeiro. Pheno- and genotypic results were concordant for 59,52% (25/42) and 78,57% (11/14) S. lentus and S. sciuri isolates, respectively. The phenotypic antimicrobial resistance pattern was evaluated and correlated to the detection of mecA and pbpD genes. Also, mecA sequences from sciuri group strains were compared to others species in order to evaluate the degree of similarity among them. Although all S. sciuri isolates tested positive for pbpD, no correlation was observed with antimicrobial resistance for isolates that presented only mecA homologue On the other hand, isolates tested positive for mecA gene also presented phenotypic resistance in at least one assay. The alignment of the mecA gene showed that the nucleotide sequences were sorted into 2 different groups, one comprising the bovine strains and the other containing human and equine strains.A resist?ncia ? oxacilina em Staphylococcus ? decorrente da integra??o do SSCmec, onde est? localizado o gene mecA, no cromossomo bacteriano. Este gene codifica uma variante da PBP2, a PBP2a, de baixa afinidade a penicilina. O complexo mec ? tamb?m composto pelos genes mecR1 e mecI, com atividade repressora e anti-repressora, respectivamente, sobre o gene mecA. A origem do gene mecA ? desconhecida, por?m, estudos sugerem que sua fonte est? relacionada com Staphylococcus coagulase-negativo, uma vez que a presen?a de mecA e um gene hom?logo denominado pbpD foi detectada em isolados de Staphylococcus sciuri. O presente estudo avaliou 210 amostras de pele e conduto auditivo externo de camundongos e 60 swabs nasais de equinos saud?veis provenientes do Biot?rio e da Fazenda Modelo Gericin?, ambos do Instituto de Biologia do Ex?rcito, localizados no munic?pio do Rio de Janeiro. Resultados feno e genot?picos foram concordantes para 59,52% (25/42) e 78,57% (11/14) para S. lentus e S. sciuri, respectivamente. Foi analisado o fen?tipo de resist?ncia antimicrobiana e correlacionado com a detec??o dos genes mecA e pbpD, em seguida, foi avaliada a similaridade entre as sequ?ncias do gene mecA detectado nas esp?cies do Grupo sciuri com o gene presente em Staphylococcus aureus resistentes ? meticilina. Todos os isolados S. sciuri apresentaram o hom?logo do gene mecA, sem qualquer express?o fenot?pica de resist?ncia ? oxacilina. Por outro lado, os isolados que possu?am o gene mecA apresentaram resist?ncia fenot?pica em pelo menos um dos antibi?ticos testados. O alinhamento do gene mecA mostrou que as sequ?ncias de nucle?tidos foram agrupados em dois grupos diferentes, um oriundo de amostras bovinas e outro de amostras humanas e equinas.Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2020-04-20T18:42:49Z No. of bitstreams: 1 2013 - Amanda Couto Calazans Silva.pdf: 1010184 bytes, checksum: b379be07203c74be0a15486ca47041dc (MD5)Made available in DSpace on 2020-04-20T18:42:49Z (GMT). No. of bitstreams: 1 2013 - Amanda Couto Calazans Silva.pdf: 1010184 bytes, checksum: b379be07203c74be0a15486ca47041dc (MD5) Previous issue date: 2013-03-15CAPES - Coordena??o de Aperfei?oamento de Pessoal de N?vel Superiorapplication/pdfhttps://tede.ufrrj.br/retrieve/55756/2013%20-%20Amanda%20Couto%20Calazans%20Silva.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Ci?ncias Veterin?riasUFRRJBrasilInstituto de Veterin?riaADEGOKE,G.O. Comparative characteristics of Staphylococcus sciuri, Staphylococcus lentus and Staphylococcus gallinarum isolated from healthy and sick hosts. Vet. Microbiol. v. 11, p.185?189, 1986. ALTSCHUL, S.F,.; MADDEN, T.L.; SCHAFFER, A.A.; ZHANG, J.; ZHANG, Z.; MILLER, W.; LIPMAN, D.J. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucleic Acids Res. v.25, p.3389-3402, 1997. ANTIGNAC, A.;TOMASZ, A. Reconstruction of the Phenotypes of Methicilin-Resistant Staphylococcus aureus by Replacement of the Staphylococcal Cassete Chromossome mec with a Plasmid-Borne Copy of the Staphylococcus sciuri pbpD Gene. Antimicrob. Agents Chemother. v.53, n.2, p.435-441, 2008. BAGCIGIL, F.A., MOODLEY, A., BAPTISTE, K.E., JENSEN, V.F., GUARDABASSI, L. Occurrence, species distribution, antimicrobial resistance and clonality of methicillin- and erythromycin-resistant staphylococci in the nasal cavity of domestic animals. Veterinary Microbiology. v.121, p.307?315, 2007. BEHME, R.J.; SHUTTLEWORTH, R.; MCNABB, A.; COLBY, W.D. Identification of staphylococci with a self-educating system using fatty acid analysis and biochemical tests. J. Clin. Microbiol. v.34, p.3075?3084, 1996. BLACK, J.G. Microbiologia: Fundamentos e Perspectivas. 4? ed., Rio de Janeiro: Guanabara Koogan, p.370-419, 2002. BOYLE-VAVRA, S., DAUM, R. S. Community-acquired methicillin resistant Staphylococcus aureus: the role of Panton-Valentine leukocidin. Lab Invet, v.87, p. 3-9, 2008. BROWN, D.F.J. Detection of methicillin/oxacillin resistance in staphylococci. Journal Antimicrobial Chemotherapy, v.48, p.65-70, 2001. CAUWELIER, B.; GORDTS, B.; DESCHEEMAECKER, P. Evaluation of a disk diffusion method with cefoxitin (30?g) for detection of methicillin-resistant Staphylococcus aureus. European Journal Microbiology Infections Disease, v.23, p.389-392, 2004. CHAMBERS, H.F. Methicillin resistance in staphylococci: molecular and biochemical basis and clinical implications. Clinical Microbiology Revist. v.10, n.4, p.781-791, 1997. CLARKE, S.R.; DYKE, K.G.H. The signal transducer (BlaRI) and the repressor (BlaI) of the Staphylococcus aureus ?-lactamase operon are inducible. Microbiology. v.147, p. 803 810, 2001. 35 CLSI. Performance Standards for Antimicrobial Susceptibility Testing. 20th Informational Supplement, M100-S20. CLSI, Wayne, PA Clinical and Laboratory Standards Institute; 2011. COELHO S.M.O.; MENEZES R.A.; SOARES L.C.; PEREIRA I.A.; GOMES L.P.; SOUZA M.M.S. Mapeamento do Perfil de Resist?ncia e Detec??o do gene mecA em Staphylococcus aureus e Staphylococcus intermedius oxacilina-resistentes isolados de esp?cies humanas e animais. Ci?ncia Rural. v.37, n.1, p.195-200, 2007. COUTO, I.; SANCHES, I. S.; S?-LE?O, R.; LENCASTRE, H. Molecular characterization of Staphylococcus sciuri strains isolated from human. Journal of Clinical Microbiology, v.38, n.3, p.1136-1143, 2000. COUTO, I.; WU, S. W.; TOMASZ, A.; LENCASTRE, H. Development of methicillin resistance in clinical isolates of Staphylococcus sciuri by transcriptional activation of the mecA homologue native to the species. Journal of Clinical Microbiology, v.185, n.2, p.645-653, 2003. COUTO, I.; LENCASTRE, H.; SEVERINA, E.; KLOOS, W.; WEBSTER, J.A.; HUBNER, R.J.; SANCHES, I.S.; TOMASZ, A. Ubiquitous Presence of a mecA Homologue in Natural Isolates of Staphylococcus sciuri. Microbial Drug Resistance. v.2, n.4, p.377-391, 2010. CUNHA, M. L.R.S; LOPES, R.S.; RUGOLO, C. A. M.; CHALITA, L.V.A.S. Clinical significance of coagulase-negative staphylococci isolated from neonates. Journal Antimicrobial and Chemotherapy. v.48, p. 463?478, 2002. CUNY, C.; LAYER, F.; STROMMENGER, B.; WITTE, W. Rare Occurrence of Methicillin-Resistant Staphylococcus aureus CC130 with a Novel mecA Homologue in Humans in Germany. PLoS ONE ,v. 6, n. 9, 2011. DANCER, S.J. The problem with cephalosporins. Journal Antimicrobial and Chemotherapy, v.48, p.463?478, 2001. DEVRIESE, L.A.; NZUAMBE, D.; GODARD, C.Identification and characteristics of staphylococci isolated from lesions and normal skin of horses. Veterinary Microbiology. v.10, n.3, p.269-277, 1985. . FELSENSTEIN, J. Confidence limits on phylogenies: An approach using the bootstrap. Evolution, v.39, p.783?791, 1985. FERNANDES, C.J.; FERNANDES, L.A.; COLLIGNON, P. Cefoxitin resistance as a surrogate marker for the detection of methicillin-resistant Staphylococcus aureus. Journal Antimicrobial Chemotherapy, v. 55, p. 506-10, 2005. FIRTH, N.; SKURRAY, R.A. The Staphylococcus:Genetics: Accessory elements and genetic exchange. American Society for Microbiology, p. 326-338, 2000. 36 FORBES, B.A.; SAHM, D.F.; WEISSFELD, A.S. Staphylococcus, Micrococcus and Similar Organisms. In: Bayiley & Scott?s Diagnostic Microbiology, 11 ed. Mosby: USA, 2002. FRAZIER, W. C; WESHOFF, D. C. Microbiologia de los Alimentos. 4. ed. Zaragosa: Editorial Acribia, 2000. FUDA, C.; FISHER, J.; MOBASHEREY, S. ?-Lactam resistance in Staphylococcus aureus: the adaptive resistance of a plastic genome. Cellular and Molecular Life Sciences (CMLS). Vol. 62, p. 2617-1633, 2005. GOLEMI-KOTRA, D.; CHA, J.Y.; MEROUEH, S.O.; VAKULENKO, S.B.; MOBASHERY, S. Resistance to b-lactam antibiotics and its mediation by the sensor domain of the transmembrane BlaR signaling pathway in Staphylococcus aureus. Journal of Biological Chemistry, v.278, p.18419-18425, 2003. GOLLEDGE, C.L. Staphylococcus saprophyticus bacteremia. J. Infect.Dis., v.157, p.215-234, 1988. GOTZ, F. Staphylococcus and biofilms. Molecular Microbiology., v.43, p.1367?1378, 2002. GRAHAM, J. C. Comparison of PCR detection of mecA with methicillin and oxacillin disc susceptibility testing in coagulase-negative staphylococci. Journal of Antimicrobial Chemotherapy, v.45, p.111-113, 2000. GRIETHUYSEN, A. van.; LOO, I. van.; BELKUM, A. van; VANDENBROUCKEGRAULS, C.; WANNET, W.; KEULEN, P. van.; KLUYTMANS, J. Loss of the mecA Gene during Storage of Methicillin-Resistant Staphylococus aureus Strains. Journal of Clinical Microbiology, The Netherlands, v.43, n. 3, p-1361-1365, 2005. GUARDABASSI, L.; SCHWARZ, S.; LLOYD, D.H. Pet animals as reservoirs of antimicrobial-resistant bacteria. Journal of Antimicrobial and Chemotherapy. v.54, pp.321?332, 2004. HACKBARTH, C.J.; CHAMBERS, H.F. blaI and blaR1 regulate beta-lactamase and PBP 2a production in methicillin-resistant Staphylococcus aureus. Antimicrobial Agents Chemotherapy, v.37, n.(5), p. 1144-1149, 1993. HARDY, B. The issue of antibiotic use in the livestock industry: what have we learned? Animal Biotechnology, v. 13, n. (1), p. 129-147, 2002. HEIKENS, E. Comparison of genotypic and phenotypic methods for species level identification of clinical isolates of coagulase-negative staphylococci. Journal of Clinical Microbiology, v. 43, n. 5, p. 2286-2290, 2005. HEILMANN, C.; PETERS, G. Biology and pathogenicity of S. epidermidis. In: Fischetti VA; Novick RP; Ferreti JJ; Portnoy DA; Rood JI Gram-positive pathogens. Washington, D.C.: ASM Press, p.442-9, 2000. 37 HERRON-OLSON, L.; FITZGERALD, J.R.; MUSSER, J.M.; KAPUR, V. Molecular Correlates of Host Specialization in Staphylococcus aureus. PLoS ONE 2(10): e1120.doi:10.1371/journal.pone.0001120, 2007. HIGGINS, E.T., RONEY, C., CROWE, E., HYMES, C. Ideal versus ought predilections for approach and avoidance: Distinct self-regulatory systems. Journal of Personality and Social Psychology 66: 276?286, 1994. HUDOME, S.M.; FISHER, M.C. Nosocomial infections in the neonatal intensive care. Curr Opin Infect Dis 2001;14:303-7. HUSSAIN, Z. Comparison of the MicroScan system with the ?API Staph-Ident? system for species identification of coagulase-negative staphylococci. J. Clin. Microbiol., v.23, n.8, p.126-128, 1986. HUSSAIN, Z.; STOAKES, L.; MICHAEL, A.J.; GARROW, S.; FITZGERALD, V. Detection of methicillin resistance in primary blood culture isolates of coagulase negative staphylococci by PCR, slide agglutination, disk diffusion, and a commercial method. Journal of Clinical Microbiology, v.40, n.6, p.2251-2253, 2001. ITO T; KATAYAMA Y.; ASDA K.; MORI N.; TSUTSUMIMOTO K.; TIENSASITORN C.; HIRAMATSU K. Structural comparison of three types of staphylococcal cassette chromosome mec integrated in the chromosome in methicillin-resistant S. aureus. Antimicrobial Agents and Chemotherapy. v.45, pp.1323-1336, 2001. ITO, T.; MA, X.X.; TAKEUCHI, F.; OKUMA, K.; YUZAWA, H.; HIRAMATSU, K. Novel type V staphylococcal cassette chromosome mec driven by a novel cassette chromosome recombinase, ccr. Antimicrobial Agents and Chemotherapy. v.48, pp. 2637-2651, 2004. JANSEN, M.D.; BOX, A.T.A.; FLUIT, A.C. SCCmec Typing in Methicillin-Resistant Staphylococcus aureus Strains of Animal Origin. Emerging Infectious Diseases.v.15, n.1, p.136, 2009. KARAKULSKA, J.; FIJA?KOWSKI, K.; NAWROTEK, P.; POBUCEWICZ, A.; POSZUMSKI, F.; CZERNOMYSY-FUROWICZ, D. Identification and Methicillin Resistance of Coagulase-Negative Staphylococci Isolated from Nasal Cavity of Healthy Horses. The Journal of Microbiology. v.50, n.3, p.444-451, 2012. KATAYAMA, Y.; ITO, T.; HIRAMATSU, K. Genetic organization of the chromosome region surrounding mecA in clinical staphylococcal strains: role of IS431-mediated mecI deletion in expression of resistance in mecA-carrrying, low-level methicillinresistant Staphylococcus haemolyticus. Antimicrobial Agents and Chemotherapy, v. 45, n. 7, p. 1955-1963, 2001. KAWAMURA,Y.; HOU, X.G.; SULTANA, F.; HIROSE, K.; MIYAKE, M.; SHU, S.E.; EZAKI, T. Distribution of Staphylococcus species among human clinical 38 specimens and emended description of Staphylococcus caprae. J. Clin. Microbiol. v.36, p.2038?2042, 1998. KLOOS, W. E.; K. H. SCHLEIFER; SMITH, R. F. Characterization of Staphylococcus sciuri sp. nov. and its subspecies. Int. J. Syst. Bacteriol. v.26, p. 22?37, 1975. KLOOS, W.E.; SCHLEIFER, K.H. Genus IV ? Staphylococcus. Bergey?s Manual of Systematic Bacteriology. Baltimore. p. 1.013-1.035, 1994. KLOOS, W.E. Taxonomy and Systematic of Staphylococci Indigenous to Humans. In: Crossey KB, Archer LG, editors. The Staphylococci in Human Disease. New York: Churchill Livingstone. p. 113-37, 1999. KOHNER, J. P.; UHL, J.; KOLBERT, C.; PERSING, D.; COCKERILL, F. A Comparison of susceptibility testing methods with mecA Gene analysis for determining oxacilin (methicilin) resistance in clinical isolates of Staphylococcus aureus and coagulase -ative staphylococcus spp.. Journal of Clinical Microbiology. v.37. n.9 p.2952-2961, 1999. KONEMAN, E.W.; ALLEN, S.D.; JANDA, W.M; SCHRECKENBERGER, P.C.; WINN, J.R. Diagn. Microbiol. 6.ed. Rio de Janeiro: Editora MEDS, 2008. KURODA, M.; OHTA, T.; UCHIYAMA, I.; BABA T.; YUZAWA, H.I.; KOBAYASHI, L.; CUI, A.; OGUCHI, K.; AOKI, Y.; NAGAI, J. Plasmid-mediated resistance to vancomycin and teicoplanin in Enterococcus faecium. New Englang Journal Medicine, v.319, p.157-61, 2001. LEE, J.H. Methicillin (Oxacillin)-resistant Staphylococcus aureus strains isolated from major food animals and their potential transmission to humans. Appl Environ Microbiol. v. 69, p.6489?6494, 2003. LOWY, F.D. Antimicrobial resistance: the example of Staphylococcus aureus. Journal Clinical Investigation, v.111, p.1265-1273, 2003. LUDWIG, W.; SCHLEIFER, K-H. & WHITMAN, W. B. 2009. Revised Road Map to the Phylum Firmicutes. http://www.bergeys.org/outlines/Bergeys_Vol_3_Outline.pdf, pp. 1-32 MA, X. X.; ITO, T.; TIASANSITORN, C.; JAMKLANG, M.; CHONGTRAKOOL, P.; BOYLE-VAVRA, S.; DAUM, R. S.; HIRAMATSU, K. Novel type of staphylococcal cassette chromosome mec identifield in community-acquired methicillin-resistant Staphylococcus aureus strains. Antimicrob Agents Chemother. 2002; 46 (4): 1147-52. MANIE, T. Antimicrobial resistance of bacteria isolated from slaughtered and retail chickens in south Africa. Letters in Applied Microbiology. v. 26, p. 253-258, 1997. MARTINS, R. P.; MARQUES, M. R. H.; NETO, A .C. Etiologia da mastite subcl?nica em vacas do rebanho de uma queijaria em Nossa Senhora do Livramento, MT. Revista Higiene Alimentar, S?o Paulo, v. 20, n. 13, p. 104-110, 2006. 39 MARTINEAU, F.; PICARD, F.J.; LANSAC, N.; MENARD, C.; ROY, P.H.; OUELLETTE, M.; BERGERON, M.G. Correlation between the resistance genotype determined by multiplex PCR assays and the antibiotic susceptibility patterns of Staphylococcus aureus and Staphylococcus epidermidis. Antimicrobial Agents and Chemotheraphy, v. 44, p.231?238, 2000. MCEWEN, S.A.; FEDORKA-CRAY, P.J. Antimicrobial Use and Resistance in Animals Clinical Infectious Disease, v.34, n.(3), p.93-106, 2002. McKINNEY, T.K.; SHARMA, V.K.; CRAIG, W.A.; ARCHER, G.L. Transcription of the gene mediating methicillin resistance in Staphylococcus aureus (mecA) is corepressed but not coinduced by cognate mecA and ?-lactamase regulators. Journal Bacteriology, v.183, n.23, p. 6862-8, 2001 MOELLERING, R.C. The problem of complicated skin and skin structure infections: the need for new agents. J Antimicrob Chemother, v.65, n.4, p.3-8, 2010. MORENO, L. V. Antibiotic residues and drugs resistant bacteria in beef and chicken tissues. Journal of Food Science, v. 55, n. 3, p. 632-635, 1990. MORSE, S.I. Estafilococos. In: Braude AI, Davis CE, Fierer J, editors. Microbiologia Cl?nica. Buenos Aires: Panamericana; 1984. p. 313-20. MOTA, R.A.; SILVA, R.A.; FREITAS, M.F.L.; PORTO, W.J.N.; SILVA, L.B.G. Utiliza??o indiscriminada de antimicrobianos e sua contribui??o a multirresit?ncia bacteriana. Brazilian Journal veterinary Research animal Science. v. 42, n. 6, p. 465-470, 2005. MURAKAMI, K.W.; MINAMIDE, K.; WADA, W.; NAKAMURA, E.; TERAOKA, H.; WATANBE, S. Identification of methicillin resistant strains of staphylococci by polymerase chain reaction. Journal of Clinical Microbiology. v.29, pp.2240-2244, 1991. NATIONAL CENTER FOR BIOTECHNOLOGY INFORMATION. TaxBrowser, Washington, 12 dez. 2006. Dispon?vel em: http://www.ncbi.nlm.nih.gov/sites/entrez?db=taxonomy. Acesso em: 20 out. 2011. OLIVEIRA, D.C., MILHEIRI?O, C., DE LENCASTRE, H. Redefining a structural variant of staphylococcal cassette chromosome mec, SCCmec type VI. Antimicrobial Agents and Chemotherapy. v.50, pp.3457-3459, 2006. OLIVEIRA, N. J. Antibi?ticos promotores de crescimento na produ??o animal. In: 4? Encontro de zootecnista do norte de minas: novas perspectivas mercadol?gicas, 2008, Montes Claros. Anais da UFMG, p. 89-115, v. 42, 2008. PEREIRA, I. A. Processos infecciosos de animais de companhia: uma abordagem sobre fatores de virul?ncia em Staphylococcus spp. e resist?ncia ? azitromicina e oxacilina como modelo de estudo.166 p. Tese (Doutorado em Ci?ncias Veterin?rias). Instituto de Veterin?ria, Departamento de Parasitologia Animal, Universidade Federal Rural do Rio de Janeiro, Serop?dica, RJ, 2010. 40 PRESCOTT, M.L., HARLEY, J.P. AND KLEIN, D.A. Procaryotic cell structure and function in Microbiology. WCB . p 33-72, 1996. ROSATO, A.E.; KREISWIRTH, B.N.; CRAIG,W.A.; EISNER, W.; CLIMO, M.; ARCHER, G.L. mecA-blaZ Corepressors in Clinical Staphylococcus aureus Isolates. Antimicrobial Agents and Chemotherapy, v.47, n.(4), p. 1460?1463, 2003. SAKAI, H. Simultaneous detection of Staphylococcus aureus and coagulasenegative staphylococci in positive blood cultures by real-time PCR with two fluorescence resonance energy transfer probe sets. Journal of Clinical Microbiology, v.42, n.12, p.5739-5744, 2004. SAMBROOK, J.; FRITSCH, E. F.; MANIATIS, T. Molecular Cloning: a laboratory manual. New York: Cold Spring Harbor Laboratory Press. 2002. SEVERIN,A.; WU, S.W; TABEI, K.; TOMASZ. A. High-Level ?-Lactam Resistance and Cell Wall Synthesis Catalyzed by the mecA Homologue of the Staphylococcus sciuri Introduced into Staphylococcus aureus. J. Bacteriol. v.187, n.19, p.6651-6658, 2005. SILVA, C.C.M. Resist?ncia a Antimicrobianos em Staphylococcus aureus Isolados de Gado de Leite Tese apresentada ? Universidade Federal de Vi?osa, como parte das exig?ncias do Programa de P?s-Gradua??o em Microbiologia Agr?cola, para obten??o do t?tulo de Doctor Scientiae. 2008. SNARY, E.L.; KELLY, L.A.; DAVISON, H.C.; TEALE, C.; WOOLDRIDGE, M. Antimicrobial resistance: a microbial risk assessment perspective. Journal of Antimicrobial Chemotherapy. v.53, n.6, pp.906-917, 2004. SOARES, L. C. Correla??o entre marcadores fenot?picos e genot?picos de virul?ncia e resit?ncia ? oxacilina ea Staphylococcus spp. coagulase-negativos isolados a partir de mastite bovina. 82 p. Tese (Doutorado em Ci?ncias Veterin?rias). Instituto de Veterin?ria. Departamento de Parasitologia Animal, Universidade Federal Rural do Rio de Janeiro, Serop?dica, RJ, 2010. SOUZA, M. M. S., COELHO, S. M. O., PEREIRA, I. A., SOARES, L. C., PRIBUL, B. R., COELHO, I. S. Antibiotic resistance in Staphylococcus species of animal origin. Antibiotic Resistence, 2012, in press. STAPLETON, P.D.; TAYLOR, P.W. Methicillin resistance in Staphylococcus aureus mechanisms and modulation. Scientific Program. v.85, n.(1), p. 57?72, 2002 STEPANOVIC, S.; DAKIC, I.; MORRISON, D.; HAUSCHILD, T.; JEZEK, P.; PETR?S, P.; MARTEL,A.; VUKOVIC, D.; SHITTU, A.; DEVRIESE, L.A. Identification and Characterization of Clinical Isolates of Members of the Staphylococcus sciuri Group, Journal of Clinical Microbiology. v.43, n.2, p.956-958, 2005. 41 SUZUKI, E.; KUWAHARA-ARAI, K.; RICHARDSON, J. F.; HIRAMATSU, K. Distribution of mec regulator genes in methicillin-resistant Staphylococcus clinical strains. Antimicrobial Agents and Chemotherpy. v.37, p.1219?1226, 1993. SUYAMA, M.; BORK, P. Evolution of prokaryotic gene order: genome rearrangements in closely related species. Trends Genetics, v. 17, p.10?13, 2001. SWENSON, J.M.; TENOVER, F.C.; WILLIAMS, P.P.; KILLGORE, G.; Results of disk diffusion testing with cefoxitin correlate with presence of mecA in Staphylococcus spp. Journal of Clinical Microbiology. v.43, n.8, p.3818-3823, 2005. TENOVER, F. C. (2008). Vancomycin-resistant staphylococcus aureus: a perfect but geographically limited storm? Clinical Infectious Disease; v. 46, p. 675?677, 2008. TOPLEY, W.W.C. Wilson GS. Staphylococcus and Micrococcus. In: Principles of Bacteriology, Virology and Immunity. 6th ed. London: E. Arnold; 1976. p.764-801. VELASCO, D. Evaluation of different methods for detecting methicillin (oxacillin) resistance in Staphylococcus aureus. Journal Antimicrobial Chemotherapy, v. 55, p. 379-82, 2005. VON EIFF, C.; PETERS, G. HEILMANN, C. Pathogenesis of infections due to coagulase-negative staphylococci. Lancet of Infectious Disease. v. 2, p. 677-685, 2004. WALKER. J.; BORROW, R.; GOERING, R.V.; EGERTON, S.; FOX, A.; OPPENHEIN, B.A. Subtyping of methicillin-resistant Staphylococcus aureus isolates from the North-West of England: a comparison of standardized pulsed-field gel electrophoresis with bacteriophage typing including an inter-laboratory reproducibility study. Journal of Medical Microbiology. v.48, p.297-301, 1999. WEESE, J. S.; ROUSSEAU, J.; TRAUB-DARATZ, J.L.; WILLEY, B.M., MCGREER, A.J.; LOW, D.E. Community-associated methicillin-resistant Staphylococcus aureus in horses and humans who work with horses. Jouranl of The American Vetenary Medicine Association. v.226, pp.580?583, 2005. WELLER, T. M. The distribution of mecA, mecRI and mecI and sequence analysis of mecI and the mec promoter region in staphylococci expressing resistance to methicillin. Journal Antimicrobial Chemotherapy, v.43, p.15?22, 1999. WILKE, M.S.; LOVERING, A.L.; STRYNADKA, N.C.J. b-Lactam antibiotic resistance: a current structural perspective. Current Opinion in Microbiology, v.8, p.525?533, 2005. WITTE, W.; KRESKEN. M.; BRAULKE, C.; CUNY, C. Increasing incidence and widespread dissemination of methicilin-resistant Staphylococcus aureus (MRSA) in hospitals in central Europe, with special reference to German hospitals. Clinical of Microbiology Infections. v.3, n.40, p.414-422, 1999. 42 YASUDA, R.; KAWANO, J.; MATSUO, E.; MASUDA, T.; SHIMIZU, A.; ANZAI, T.; HASHIKURA, S. Distribution of mecA-harboring staphylococci in healthy horses. Journal Veterinary Medicine Science. v.69, n.4, p.821-827, 2002. YOUNG, H. K. Do nonclinical uses of antibiotics make a different? Infectious Control Hospital Epidemiology. v. 15, p. 484-487, 1994. ZHANG, L.; GRAY, L.; NOVICK, R.P. & JI, G. Transmembrane topology of AgrB, the protein involved in the post-translational modification of AgrD in Staphylococcus aureus. Journal of Biology and Chemmistry.v.277, p.34736-34742, 2004. ZHOU, Y.; ANTIGNAC, A.; WU, S.W.; TOMASZ, A. Penicillin-binding proteins and cell wall composition in ?-lactam-sensitive and -resistant strains of Staphylococcus sciuri. Journal of Bacteriology. 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dc.title.por.fl_str_mv Caracteriza??o de Staphylococcus do grupo sciuri e analise fenogenot?pica da resist?ncia ? oxacilina em isolados de roedores e equinos do Instituto Biol?gico do Ex?rcito
dc.title.alternative.eng.fl_str_mv Phenogenotypic characterization of Staphylococcus sciuri group and oxacillin-resistance analysis in isolates from rodents ande quines of Army Biologic Institute
title Caracteriza??o de Staphylococcus do grupo sciuri e analise fenogenot?pica da resist?ncia ? oxacilina em isolados de roedores e equinos do Instituto Biol?gico do Ex?rcito
spellingShingle Caracteriza??o de Staphylococcus do grupo sciuri e analise fenogenot?pica da resist?ncia ? oxacilina em isolados de roedores e equinos do Instituto Biol?gico do Ex?rcito
Silva, Amanda Couto Calazans
Staphylococcus sciuri
gene mecA
resist?ncia antimicrobiana
Staphylococcus sciuri
mecA gene
antimicrobial resistance
Medicina Veterin?ria
title_short Caracteriza??o de Staphylococcus do grupo sciuri e analise fenogenot?pica da resist?ncia ? oxacilina em isolados de roedores e equinos do Instituto Biol?gico do Ex?rcito
title_full Caracteriza??o de Staphylococcus do grupo sciuri e analise fenogenot?pica da resist?ncia ? oxacilina em isolados de roedores e equinos do Instituto Biol?gico do Ex?rcito
title_fullStr Caracteriza??o de Staphylococcus do grupo sciuri e analise fenogenot?pica da resist?ncia ? oxacilina em isolados de roedores e equinos do Instituto Biol?gico do Ex?rcito
title_full_unstemmed Caracteriza??o de Staphylococcus do grupo sciuri e analise fenogenot?pica da resist?ncia ? oxacilina em isolados de roedores e equinos do Instituto Biol?gico do Ex?rcito
title_sort Caracteriza??o de Staphylococcus do grupo sciuri e analise fenogenot?pica da resist?ncia ? oxacilina em isolados de roedores e equinos do Instituto Biol?gico do Ex?rcito
author Silva, Amanda Couto Calazans
author_facet Silva, Amanda Couto Calazans
author_role author
dc.contributor.advisor1.fl_str_mv Souza, Miliane Moreira Soares de
dc.contributor.advisor1ID.fl_str_mv 010.761.987-32
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/0865211214618618
dc.contributor.advisor-co1.fl_str_mv Coelho, Shana de Mattos de Oliveira
dc.contributor.authorID.fl_str_mv 106.751.817-71
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/2160878993935525
dc.contributor.author.fl_str_mv Silva, Amanda Couto Calazans
contributor_str_mv Souza, Miliane Moreira Soares de
Coelho, Shana de Mattos de Oliveira
dc.subject.por.fl_str_mv Staphylococcus sciuri
gene mecA
resist?ncia antimicrobiana
topic Staphylococcus sciuri
gene mecA
resist?ncia antimicrobiana
Staphylococcus sciuri
mecA gene
antimicrobial resistance
Medicina Veterin?ria
dc.subject.eng.fl_str_mv Staphylococcus sciuri
mecA gene
antimicrobial resistance
dc.subject.cnpq.fl_str_mv Medicina Veterin?ria
description Oxacillin/methicillin-resistance in Staphylococcus is related to the integration of SSCmec, containing the mec complex, in the bacterial chromosome. The mecA gene codifies a variant of PBP2, termed PBP2a or PBP2?, whose affinity with the beta-lactamic antibiotics is very low. Complex mec contains mecA and its regulatory genes mecR1 and mecI. Its origin is still unknown, although evidences support that it is related to coagulase-negative staphylococci, once mecA and a homologue gene, pbpD, were both detected in Staphylococcus sciuri species group. The present work evaluated 210 samples of skin and ear swabs from rodents and 60 nasal swabs from equines of Army Biologic Institute, Rio de Janeiro. Pheno- and genotypic results were concordant for 59,52% (25/42) and 78,57% (11/14) S. lentus and S. sciuri isolates, respectively. The phenotypic antimicrobial resistance pattern was evaluated and correlated to the detection of mecA and pbpD genes. Also, mecA sequences from sciuri group strains were compared to others species in order to evaluate the degree of similarity among them. Although all S. sciuri isolates tested positive for pbpD, no correlation was observed with antimicrobial resistance for isolates that presented only mecA homologue On the other hand, isolates tested positive for mecA gene also presented phenotypic resistance in at least one assay. The alignment of the mecA gene showed that the nucleotide sequences were sorted into 2 different groups, one comprising the bovine strains and the other containing human and equine strains.
publishDate 2013
dc.date.issued.fl_str_mv 2013-03-15
dc.date.accessioned.fl_str_mv 2020-04-20T18:42:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA, Amanda Couto Calazans. Caracteriza??o de Staphylococcus do grupo sciuri e analise fenogenot?pica da resist?ncia ? oxacilina em isolados de roedores e equinos do Instituto Biol?gico do Ex?rcito. 2013. 42 f. Disserta??o (Mestrado em Ci?ncias Veterin?rias) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2013.
dc.identifier.uri.fl_str_mv https://tede.ufrrj.br/jspui/handle/jspui/3443
identifier_str_mv SILVA, Amanda Couto Calazans. Caracteriza??o de Staphylococcus do grupo sciuri e analise fenogenot?pica da resist?ncia ? oxacilina em isolados de roedores e equinos do Instituto Biol?gico do Ex?rcito. 2013. 42 f. Disserta??o (Mestrado em Ci?ncias Veterin?rias) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2013.
url https://tede.ufrrj.br/jspui/handle/jspui/3443
dc.language.iso.fl_str_mv por
language por
dc.relation.references.por.fl_str_mv ADEGOKE,G.O. Comparative characteristics of Staphylococcus sciuri, Staphylococcus lentus and Staphylococcus gallinarum isolated from healthy and sick hosts. Vet. Microbiol. v. 11, p.185?189, 1986. ALTSCHUL, S.F,.; MADDEN, T.L.; SCHAFFER, A.A.; ZHANG, J.; ZHANG, Z.; MILLER, W.; LIPMAN, D.J. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucleic Acids Res. v.25, p.3389-3402, 1997. ANTIGNAC, A.;TOMASZ, A. Reconstruction of the Phenotypes of Methicilin-Resistant Staphylococcus aureus by Replacement of the Staphylococcal Cassete Chromossome mec with a Plasmid-Borne Copy of the Staphylococcus sciuri pbpD Gene. Antimicrob. Agents Chemother. v.53, n.2, p.435-441, 2008. BAGCIGIL, F.A., MOODLEY, A., BAPTISTE, K.E., JENSEN, V.F., GUARDABASSI, L. Occurrence, species distribution, antimicrobial resistance and clonality of methicillin- and erythromycin-resistant staphylococci in the nasal cavity of domestic animals. Veterinary Microbiology. v.121, p.307?315, 2007. BEHME, R.J.; SHUTTLEWORTH, R.; MCNABB, A.; COLBY, W.D. Identification of staphylococci with a self-educating system using fatty acid analysis and biochemical tests. J. Clin. Microbiol. v.34, p.3075?3084, 1996. BLACK, J.G. Microbiologia: Fundamentos e Perspectivas. 4? ed., Rio de Janeiro: Guanabara Koogan, p.370-419, 2002. BOYLE-VAVRA, S., DAUM, R. S. Community-acquired methicillin resistant Staphylococcus aureus: the role of Panton-Valentine leukocidin. Lab Invet, v.87, p. 3-9, 2008. BROWN, D.F.J. Detection of methicillin/oxacillin resistance in staphylococci. Journal Antimicrobial Chemotherapy, v.48, p.65-70, 2001. CAUWELIER, B.; GORDTS, B.; DESCHEEMAECKER, P. Evaluation of a disk diffusion method with cefoxitin (30?g) for detection of methicillin-resistant Staphylococcus aureus. European Journal Microbiology Infections Disease, v.23, p.389-392, 2004. CHAMBERS, H.F. Methicillin resistance in staphylococci: molecular and biochemical basis and clinical implications. Clinical Microbiology Revist. v.10, n.4, p.781-791, 1997. CLARKE, S.R.; DYKE, K.G.H. The signal transducer (BlaRI) and the repressor (BlaI) of the Staphylococcus aureus ?-lactamase operon are inducible. Microbiology. v.147, p. 803 810, 2001. 35 CLSI. Performance Standards for Antimicrobial Susceptibility Testing. 20th Informational Supplement, M100-S20. CLSI, Wayne, PA Clinical and Laboratory Standards Institute; 2011. COELHO S.M.O.; MENEZES R.A.; SOARES L.C.; PEREIRA I.A.; GOMES L.P.; SOUZA M.M.S. Mapeamento do Perfil de Resist?ncia e Detec??o do gene mecA em Staphylococcus aureus e Staphylococcus intermedius oxacilina-resistentes isolados de esp?cies humanas e animais. Ci?ncia Rural. v.37, n.1, p.195-200, 2007. COUTO, I.; SANCHES, I. S.; S?-LE?O, R.; LENCASTRE, H. Molecular characterization of Staphylococcus sciuri strains isolated from human. Journal of Clinical Microbiology, v.38, n.3, p.1136-1143, 2000. COUTO, I.; WU, S. W.; TOMASZ, A.; LENCASTRE, H. Development of methicillin resistance in clinical isolates of Staphylococcus sciuri by transcriptional activation of the mecA homologue native to the species. Journal of Clinical Microbiology, v.185, n.2, p.645-653, 2003. COUTO, I.; LENCASTRE, H.; SEVERINA, E.; KLOOS, W.; WEBSTER, J.A.; HUBNER, R.J.; SANCHES, I.S.; TOMASZ, A. Ubiquitous Presence of a mecA Homologue in Natural Isolates of Staphylococcus sciuri. Microbial Drug Resistance. v.2, n.4, p.377-391, 2010. CUNHA, M. L.R.S; LOPES, R.S.; RUGOLO, C. A. M.; CHALITA, L.V.A.S. Clinical significance of coagulase-negative staphylococci isolated from neonates. Journal Antimicrobial and Chemotherapy. v.48, p. 463?478, 2002. CUNY, C.; LAYER, F.; STROMMENGER, B.; WITTE, W. Rare Occurrence of Methicillin-Resistant Staphylococcus aureus CC130 with a Novel mecA Homologue in Humans in Germany. PLoS ONE ,v. 6, n. 9, 2011. DANCER, S.J. The problem with cephalosporins. Journal Antimicrobial and Chemotherapy, v.48, p.463?478, 2001. DEVRIESE, L.A.; NZUAMBE, D.; GODARD, C.Identification and characteristics of staphylococci isolated from lesions and normal skin of horses. Veterinary Microbiology. v.10, n.3, p.269-277, 1985. . FELSENSTEIN, J. Confidence limits on phylogenies: An approach using the bootstrap. Evolution, v.39, p.783?791, 1985. FERNANDES, C.J.; FERNANDES, L.A.; COLLIGNON, P. Cefoxitin resistance as a surrogate marker for the detection of methicillin-resistant Staphylococcus aureus. Journal Antimicrobial Chemotherapy, v. 55, p. 506-10, 2005. FIRTH, N.; SKURRAY, R.A. The Staphylococcus:Genetics: Accessory elements and genetic exchange. American Society for Microbiology, p. 326-338, 2000. 36 FORBES, B.A.; SAHM, D.F.; WEISSFELD, A.S. Staphylococcus, Micrococcus and Similar Organisms. In: Bayiley & Scott?s Diagnostic Microbiology, 11 ed. Mosby: USA, 2002. FRAZIER, W. C; WESHOFF, D. C. Microbiologia de los Alimentos. 4. ed. Zaragosa: Editorial Acribia, 2000. FUDA, C.; FISHER, J.; MOBASHEREY, S. ?-Lactam resistance in Staphylococcus aureus: the adaptive resistance of a plastic genome. Cellular and Molecular Life Sciences (CMLS). Vol. 62, p. 2617-1633, 2005. GOLEMI-KOTRA, D.; CHA, J.Y.; MEROUEH, S.O.; VAKULENKO, S.B.; MOBASHERY, S. Resistance to b-lactam antibiotics and its mediation by the sensor domain of the transmembrane BlaR signaling pathway in Staphylococcus aureus. Journal of Biological Chemistry, v.278, p.18419-18425, 2003. GOLLEDGE, C.L. Staphylococcus saprophyticus bacteremia. J. Infect.Dis., v.157, p.215-234, 1988. GOTZ, F. Staphylococcus and biofilms. Molecular Microbiology., v.43, p.1367?1378, 2002. GRAHAM, J. C. Comparison of PCR detection of mecA with methicillin and oxacillin disc susceptibility testing in coagulase-negative staphylococci. Journal of Antimicrobial Chemotherapy, v.45, p.111-113, 2000. GRIETHUYSEN, A. van.; LOO, I. van.; BELKUM, A. van; VANDENBROUCKEGRAULS, C.; WANNET, W.; KEULEN, P. van.; KLUYTMANS, J. Loss of the mecA Gene during Storage of Methicillin-Resistant Staphylococus aureus Strains. Journal of Clinical Microbiology, The Netherlands, v.43, n. 3, p-1361-1365, 2005. GUARDABASSI, L.; SCHWARZ, S.; LLOYD, D.H. Pet animals as reservoirs of antimicrobial-resistant bacteria. Journal of Antimicrobial and Chemotherapy. v.54, pp.321?332, 2004. HACKBARTH, C.J.; CHAMBERS, H.F. blaI and blaR1 regulate beta-lactamase and PBP 2a production in methicillin-resistant Staphylococcus aureus. Antimicrobial Agents Chemotherapy, v.37, n.(5), p. 1144-1149, 1993. HARDY, B. The issue of antibiotic use in the livestock industry: what have we learned? Animal Biotechnology, v. 13, n. (1), p. 129-147, 2002. HEIKENS, E. Comparison of genotypic and phenotypic methods for species level identification of clinical isolates of coagulase-negative staphylococci. Journal of Clinical Microbiology, v. 43, n. 5, p. 2286-2290, 2005. HEILMANN, C.; PETERS, G. Biology and pathogenicity of S. epidermidis. In: Fischetti VA; Novick RP; Ferreti JJ; Portnoy DA; Rood JI Gram-positive pathogens. Washington, D.C.: ASM Press, p.442-9, 2000. 37 HERRON-OLSON, L.; FITZGERALD, J.R.; MUSSER, J.M.; KAPUR, V. Molecular Correlates of Host Specialization in Staphylococcus aureus. PLoS ONE 2(10): e1120.doi:10.1371/journal.pone.0001120, 2007. HIGGINS, E.T., RONEY, C., CROWE, E., HYMES, C. Ideal versus ought predilections for approach and avoidance: Distinct self-regulatory systems. Journal of Personality and Social Psychology 66: 276?286, 1994. HUDOME, S.M.; FISHER, M.C. Nosocomial infections in the neonatal intensive care. Curr Opin Infect Dis 2001;14:303-7. HUSSAIN, Z. Comparison of the MicroScan system with the ?API Staph-Ident? system for species identification of coagulase-negative staphylococci. J. Clin. Microbiol., v.23, n.8, p.126-128, 1986. HUSSAIN, Z.; STOAKES, L.; MICHAEL, A.J.; GARROW, S.; FITZGERALD, V. Detection of methicillin resistance in primary blood culture isolates of coagulase negative staphylococci by PCR, slide agglutination, disk diffusion, and a commercial method. Journal of Clinical Microbiology, v.40, n.6, p.2251-2253, 2001. ITO T; KATAYAMA Y.; ASDA K.; MORI N.; TSUTSUMIMOTO K.; TIENSASITORN C.; HIRAMATSU K. Structural comparison of three types of staphylococcal cassette chromosome mec integrated in the chromosome in methicillin-resistant S. aureus. Antimicrobial Agents and Chemotherapy. v.45, pp.1323-1336, 2001. ITO, T.; MA, X.X.; TAKEUCHI, F.; OKUMA, K.; YUZAWA, H.; HIRAMATSU, K. Novel type V staphylococcal cassette chromosome mec driven by a novel cassette chromosome recombinase, ccr. Antimicrobial Agents and Chemotherapy. v.48, pp. 2637-2651, 2004. JANSEN, M.D.; BOX, A.T.A.; FLUIT, A.C. SCCmec Typing in Methicillin-Resistant Staphylococcus aureus Strains of Animal Origin. Emerging Infectious Diseases.v.15, n.1, p.136, 2009. KARAKULSKA, J.; FIJA?KOWSKI, K.; NAWROTEK, P.; POBUCEWICZ, A.; POSZUMSKI, F.; CZERNOMYSY-FUROWICZ, D. Identification and Methicillin Resistance of Coagulase-Negative Staphylococci Isolated from Nasal Cavity of Healthy Horses. The Journal of Microbiology. v.50, n.3, p.444-451, 2012. KATAYAMA, Y.; ITO, T.; HIRAMATSU, K. Genetic organization of the chromosome region surrounding mecA in clinical staphylococcal strains: role of IS431-mediated mecI deletion in expression of resistance in mecA-carrrying, low-level methicillinresistant Staphylococcus haemolyticus. Antimicrobial Agents and Chemotherapy, v. 45, n. 7, p. 1955-1963, 2001. KAWAMURA,Y.; HOU, X.G.; SULTANA, F.; HIROSE, K.; MIYAKE, M.; SHU, S.E.; EZAKI, T. Distribution of Staphylococcus species among human clinical 38 specimens and emended description of Staphylococcus caprae. J. Clin. Microbiol. v.36, p.2038?2042, 1998. KLOOS, W. E.; K. H. SCHLEIFER; SMITH, R. F. Characterization of Staphylococcus sciuri sp. nov. and its subspecies. Int. J. Syst. Bacteriol. v.26, p. 22?37, 1975. KLOOS, W.E.; SCHLEIFER, K.H. Genus IV ? Staphylococcus. Bergey?s Manual of Systematic Bacteriology. Baltimore. p. 1.013-1.035, 1994. KLOOS, W.E. Taxonomy and Systematic of Staphylococci Indigenous to Humans. In: Crossey KB, Archer LG, editors. The Staphylococci in Human Disease. New York: Churchill Livingstone. p. 113-37, 1999. KOHNER, J. P.; UHL, J.; KOLBERT, C.; PERSING, D.; COCKERILL, F. A Comparison of susceptibility testing methods with mecA Gene analysis for determining oxacilin (methicilin) resistance in clinical isolates of Staphylococcus aureus and coagulase -ative staphylococcus spp.. Journal of Clinical Microbiology. v.37. n.9 p.2952-2961, 1999. KONEMAN, E.W.; ALLEN, S.D.; JANDA, W.M; SCHRECKENBERGER, P.C.; WINN, J.R. Diagn. Microbiol. 6.ed. Rio de Janeiro: Editora MEDS, 2008. KURODA, M.; OHTA, T.; UCHIYAMA, I.; BABA T.; YUZAWA, H.I.; KOBAYASHI, L.; CUI, A.; OGUCHI, K.; AOKI, Y.; NAGAI, J. Plasmid-mediated resistance to vancomycin and teicoplanin in Enterococcus faecium. New Englang Journal Medicine, v.319, p.157-61, 2001. LEE, J.H. Methicillin (Oxacillin)-resistant Staphylococcus aureus strains isolated from major food animals and their potential transmission to humans. Appl Environ Microbiol. v. 69, p.6489?6494, 2003. LOWY, F.D. Antimicrobial resistance: the example of Staphylococcus aureus. Journal Clinical Investigation, v.111, p.1265-1273, 2003. LUDWIG, W.; SCHLEIFER, K-H. & WHITMAN, W. B. 2009. Revised Road Map to the Phylum Firmicutes. http://www.bergeys.org/outlines/Bergeys_Vol_3_Outline.pdf, pp. 1-32 MA, X. X.; ITO, T.; TIASANSITORN, C.; JAMKLANG, M.; CHONGTRAKOOL, P.; BOYLE-VAVRA, S.; DAUM, R. S.; HIRAMATSU, K. Novel type of staphylococcal cassette chromosome mec identifield in community-acquired methicillin-resistant Staphylococcus aureus strains. Antimicrob Agents Chemother. 2002; 46 (4): 1147-52. MANIE, T. Antimicrobial resistance of bacteria isolated from slaughtered and retail chickens in south Africa. Letters in Applied Microbiology. v. 26, p. 253-258, 1997. MARTINS, R. P.; MARQUES, M. R. H.; NETO, A .C. Etiologia da mastite subcl?nica em vacas do rebanho de uma queijaria em Nossa Senhora do Livramento, MT. Revista Higiene Alimentar, S?o Paulo, v. 20, n. 13, p. 104-110, 2006. 39 MARTINEAU, F.; PICARD, F.J.; LANSAC, N.; MENARD, C.; ROY, P.H.; OUELLETTE, M.; BERGERON, M.G. Correlation between the resistance genotype determined by multiplex PCR assays and the antibiotic susceptibility patterns of Staphylococcus aureus and Staphylococcus epidermidis. Antimicrobial Agents and Chemotheraphy, v. 44, p.231?238, 2000. MCEWEN, S.A.; FEDORKA-CRAY, P.J. Antimicrobial Use and Resistance in Animals Clinical Infectious Disease, v.34, n.(3), p.93-106, 2002. McKINNEY, T.K.; SHARMA, V.K.; CRAIG, W.A.; ARCHER, G.L. Transcription of the gene mediating methicillin resistance in Staphylococcus aureus (mecA) is corepressed but not coinduced by cognate mecA and ?-lactamase regulators. Journal Bacteriology, v.183, n.23, p. 6862-8, 2001 MOELLERING, R.C. The problem of complicated skin and skin structure infections: the need for new agents. J Antimicrob Chemother, v.65, n.4, p.3-8, 2010. MORENO, L. V. Antibiotic residues and drugs resistant bacteria in beef and chicken tissues. Journal of Food Science, v. 55, n. 3, p. 632-635, 1990. MORSE, S.I. Estafilococos. In: Braude AI, Davis CE, Fierer J, editors. Microbiologia Cl?nica. Buenos Aires: Panamericana; 1984. p. 313-20. MOTA, R.A.; SILVA, R.A.; FREITAS, M.F.L.; PORTO, W.J.N.; SILVA, L.B.G. Utiliza??o indiscriminada de antimicrobianos e sua contribui??o a multirresit?ncia bacteriana. Brazilian Journal veterinary Research animal Science. v. 42, n. 6, p. 465-470, 2005. MURAKAMI, K.W.; MINAMIDE, K.; WADA, W.; NAKAMURA, E.; TERAOKA, H.; WATANBE, S. Identification of methicillin resistant strains of staphylococci by polymerase chain reaction. Journal of Clinical Microbiology. v.29, pp.2240-2244, 1991. NATIONAL CENTER FOR BIOTECHNOLOGY INFORMATION. TaxBrowser, Washington, 12 dez. 2006. Dispon?vel em: http://www.ncbi.nlm.nih.gov/sites/entrez?db=taxonomy. Acesso em: 20 out. 2011. OLIVEIRA, D.C., MILHEIRI?O, C., DE LENCASTRE, H. Redefining a structural variant of staphylococcal cassette chromosome mec, SCCmec type VI. Antimicrobial Agents and Chemotherapy. v.50, pp.3457-3459, 2006. OLIVEIRA, N. J. Antibi?ticos promotores de crescimento na produ??o animal. In: 4? Encontro de zootecnista do norte de minas: novas perspectivas mercadol?gicas, 2008, Montes Claros. Anais da UFMG, p. 89-115, v. 42, 2008. PEREIRA, I. A. Processos infecciosos de animais de companhia: uma abordagem sobre fatores de virul?ncia em Staphylococcus spp. e resist?ncia ? azitromicina e oxacilina como modelo de estudo.166 p. Tese (Doutorado em Ci?ncias Veterin?rias). Instituto de Veterin?ria, Departamento de Parasitologia Animal, Universidade Federal Rural do Rio de Janeiro, Serop?dica, RJ, 2010. 40 PRESCOTT, M.L., HARLEY, J.P. AND KLEIN, D.A. Procaryotic cell structure and function in Microbiology. WCB . p 33-72, 1996. ROSATO, A.E.; KREISWIRTH, B.N.; CRAIG,W.A.; EISNER, W.; CLIMO, M.; ARCHER, G.L. mecA-blaZ Corepressors in Clinical Staphylococcus aureus Isolates. Antimicrobial Agents and Chemotherapy, v.47, n.(4), p. 1460?1463, 2003. SAKAI, H. Simultaneous detection of Staphylococcus aureus and coagulasenegative staphylococci in positive blood cultures by real-time PCR with two fluorescence resonance energy transfer probe sets. Journal of Clinical Microbiology, v.42, n.12, p.5739-5744, 2004. SAMBROOK, J.; FRITSCH, E. F.; MANIATIS, T. Molecular Cloning: a laboratory manual. New York: Cold Spring Harbor Laboratory Press. 2002. SEVERIN,A.; WU, S.W; TABEI, K.; TOMASZ. A. High-Level ?-Lactam Resistance and Cell Wall Synthesis Catalyzed by the mecA Homologue of the Staphylococcus sciuri Introduced into Staphylococcus aureus. J. Bacteriol. v.187, n.19, p.6651-6658, 2005. SILVA, C.C.M. Resist?ncia a Antimicrobianos em Staphylococcus aureus Isolados de Gado de Leite Tese apresentada ? Universidade Federal de Vi?osa, como parte das exig?ncias do Programa de P?s-Gradua??o em Microbiologia Agr?cola, para obten??o do t?tulo de Doctor Scientiae. 2008. SNARY, E.L.; KELLY, L.A.; DAVISON, H.C.; TEALE, C.; WOOLDRIDGE, M. Antimicrobial resistance: a microbial risk assessment perspective. Journal of Antimicrobial Chemotherapy. v.53, n.6, pp.906-917, 2004. SOARES, L. C. Correla??o entre marcadores fenot?picos e genot?picos de virul?ncia e resit?ncia ? oxacilina ea Staphylococcus spp. coagulase-negativos isolados a partir de mastite bovina. 82 p. Tese (Doutorado em Ci?ncias Veterin?rias). Instituto de Veterin?ria. Departamento de Parasitologia Animal, Universidade Federal Rural do Rio de Janeiro, Serop?dica, RJ, 2010. SOUZA, M. M. S., COELHO, S. M. O., PEREIRA, I. A., SOARES, L. C., PRIBUL, B. R., COELHO, I. S. Antibiotic resistance in Staphylococcus species of animal origin. Antibiotic Resistence, 2012, in press. STAPLETON, P.D.; TAYLOR, P.W. Methicillin resistance in Staphylococcus aureus mechanisms and modulation. Scientific Program. v.85, n.(1), p. 57?72, 2002 STEPANOVIC, S.; DAKIC, I.; MORRISON, D.; HAUSCHILD, T.; JEZEK, P.; PETR?S, P.; MARTEL,A.; VUKOVIC, D.; SHITTU, A.; DEVRIESE, L.A. Identification and Characterization of Clinical Isolates of Members of the Staphylococcus sciuri Group, Journal of Clinical Microbiology. v.43, n.2, p.956-958, 2005. 41 SUZUKI, E.; KUWAHARA-ARAI, K.; RICHARDSON, J. F.; HIRAMATSU, K. Distribution of mec regulator genes in methicillin-resistant Staphylococcus clinical strains. Antimicrobial Agents and Chemotherpy. v.37, p.1219?1226, 1993. SUYAMA, M.; BORK, P. Evolution of prokaryotic gene order: genome rearrangements in closely related species. Trends Genetics, v. 17, p.10?13, 2001. SWENSON, J.M.; TENOVER, F.C.; WILLIAMS, P.P.; KILLGORE, G.; Results of disk diffusion testing with cefoxitin correlate with presence of mecA in Staphylococcus spp. Journal of Clinical Microbiology. v.43, n.8, p.3818-3823, 2005. TENOVER, F. C. (2008). Vancomycin-resistant staphylococcus aureus: a perfect but geographically limited storm? Clinical Infectious Disease; v. 46, p. 675?677, 2008. TOPLEY, W.W.C. Wilson GS. Staphylococcus and Micrococcus. In: Principles of Bacteriology, Virology and Immunity. 6th ed. London: E. Arnold; 1976. p.764-801. VELASCO, D. Evaluation of different methods for detecting methicillin (oxacillin) resistance in Staphylococcus aureus. Journal Antimicrobial Chemotherapy, v. 55, p. 379-82, 2005. VON EIFF, C.; PETERS, G. HEILMANN, C. Pathogenesis of infections due to coagulase-negative staphylococci. Lancet of Infectious Disease. v. 2, p. 677-685, 2004. WALKER. J.; BORROW, R.; GOERING, R.V.; EGERTON, S.; FOX, A.; OPPENHEIN, B.A. Subtyping of methicillin-resistant Staphylococcus aureus isolates from the North-West of England: a comparison of standardized pulsed-field gel electrophoresis with bacteriophage typing including an inter-laboratory reproducibility study. Journal of Medical Microbiology. v.48, p.297-301, 1999. WEESE, J. S.; ROUSSEAU, J.; TRAUB-DARATZ, J.L.; WILLEY, B.M., MCGREER, A.J.; LOW, D.E. Community-associated methicillin-resistant Staphylococcus aureus in horses and humans who work with horses. Jouranl of The American Vetenary Medicine Association. v.226, pp.580?583, 2005. WELLER, T. M. The distribution of mecA, mecRI and mecI and sequence analysis of mecI and the mec promoter region in staphylococci expressing resistance to methicillin. Journal Antimicrobial Chemotherapy, v.43, p.15?22, 1999. WILKE, M.S.; LOVERING, A.L.; STRYNADKA, N.C.J. b-Lactam antibiotic resistance: a current structural perspective. Current Opinion in Microbiology, v.8, p.525?533, 2005. WITTE, W.; KRESKEN. M.; BRAULKE, C.; CUNY, C. Increasing incidence and widespread dissemination of methicilin-resistant Staphylococcus aureus (MRSA) in hospitals in central Europe, with special reference to German hospitals. Clinical of Microbiology Infections. v.3, n.40, p.414-422, 1999. 42 YASUDA, R.; KAWANO, J.; MATSUO, E.; MASUDA, T.; SHIMIZU, A.; ANZAI, T.; HASHIKURA, S. Distribution of mecA-harboring staphylococci in healthy horses. Journal Veterinary Medicine Science. v.69, n.4, p.821-827, 2002. YOUNG, H. K. Do nonclinical uses of antibiotics make a different? Infectious Control Hospital Epidemiology. v. 15, p. 484-487, 1994. ZHANG, L.; GRAY, L.; NOVICK, R.P. & JI, G. Transmembrane topology of AgrB, the protein involved in the post-translational modification of AgrD in Staphylococcus aureus. Journal of Biology and Chemmistry.v.277, p.34736-34742, 2004. ZHOU, Y.; ANTIGNAC, A.; WU, S.W.; TOMASZ, A. Penicillin-binding proteins and cell wall composition in ?-lactam-sensitive and -resistant strains of Staphylococcus sciuri. Journal of Bacteriology. V.190, n.2, p.508-514, 2008.
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