Diagn?stico parasitol?gico com enfoque: nos parabasal?deos em matrizes su?nas de granja na microrregi?o do Vale do Paraiba fluminense, RJ

Detalhes bibliográficos
Autor(a) principal: Costa, Nelson Oscaranha Gonsales da
Data de Publicação: 2019
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
Texto Completo: https://tede.ufrrj.br/jspui/handle/jspui/5597
Resumo: This research was carried out in the Swine Laboratory of the Federal Institute of Rio de Janeiro (IFRJ), Nilo Pe?anha Campus, in the Para?ba valley microregion, southern Fluminense mesoregion, for the monitoring of Parabasalids, parasites found in swine matrices responsible for dysentery and atrophic rhinitis, despite the emphasis on technology and sanitary management of the herd studied. The objective of this work was to determine and diagnose the presence of Parabasal?deos in swine feces with and without gastrointestinal and /or respiratory symptoms, whose main route of transmission would be oral fecal. The proposed methodology was to evaluate the presence of this etiological agent in the pigs of the pigs confined in the UFRJ. For this, feces were collected from the rectal ampulla of 70 females kept at room temperature (? 30oC), which were homogenized in buffered physiological solution (PBS) at the ratio (1:10) at the Laboratory of Reproductive Pathology of the UFRRJ, , from 5.0 grams of faeces to 50 ml of PBS, kept in a bacteriological oven at ? 37 ? C for 24 hours in order to identify the protozoan. The diagnosis based on the morphology was made based on the observation of fresh assembly or through swabs stained in Quick Panoptico with the aid of a binocular optical microscope and with 10 and 40X objective. Positive samples were maintained in culture for preservation of the protozoan. As far as the results of the fresh fecal examination were, 48/70 samples positive for Parabasalids, corresponding to 68.57% of the breeding females, the main clinical picture reported was dysentery in 31/48 (64.58%) in sows, p <0.05, and no cases of atrophic rhinitis were reported. Regarding the parasitological result, 72.7% of the matrices were positive for at least one parasite, and there was case of co-infection in 75% of the infected animals. In the matrices, coccidiosis was more frequent (60.87%), followed by Parabasalidea (52.17%), Strongyloidea (47.8%) and Balantidium (26.08%). It was concluded that Parabasal?deos are present in the herd and that they play a relevant role in the infection of animals in confinement with or without the diagnosis of dysentery.
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spelling Jesus, Vera L?cia Teixeira deCPF: 708.346.107-44Jesus, Vera L?cia Teixeira deLopes, Carlos Wilson GomesCardozo, Sergian ViannaCPF: 029.290.967-56http://lattes.cnpq.br/4223689467896974Costa, Nelson Oscaranha Gonsales da2022-05-03T17:36:02Z2019-02-28COSTA, Nelson Oscaranha Gonsales da. Diagn?stico parasitol?gico com enfoque: nos parabasal?deos em matrizes su?nas de granja na microrregi?o do Vale do Para?ba fluminense, RJ. 2019. 45 f. Disserta??o (Mesytrado em Medicina Veterin?ria, Patologia Animal) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2019.https://tede.ufrrj.br/jspui/handle/jspui/5597This research was carried out in the Swine Laboratory of the Federal Institute of Rio de Janeiro (IFRJ), Nilo Pe?anha Campus, in the Para?ba valley microregion, southern Fluminense mesoregion, for the monitoring of Parabasalids, parasites found in swine matrices responsible for dysentery and atrophic rhinitis, despite the emphasis on technology and sanitary management of the herd studied. The objective of this work was to determine and diagnose the presence of Parabasal?deos in swine feces with and without gastrointestinal and /or respiratory symptoms, whose main route of transmission would be oral fecal. The proposed methodology was to evaluate the presence of this etiological agent in the pigs of the pigs confined in the UFRJ. For this, feces were collected from the rectal ampulla of 70 females kept at room temperature (? 30oC), which were homogenized in buffered physiological solution (PBS) at the ratio (1:10) at the Laboratory of Reproductive Pathology of the UFRRJ, , from 5.0 grams of faeces to 50 ml of PBS, kept in a bacteriological oven at ? 37 ? C for 24 hours in order to identify the protozoan. The diagnosis based on the morphology was made based on the observation of fresh assembly or through swabs stained in Quick Panoptico with the aid of a binocular optical microscope and with 10 and 40X objective. Positive samples were maintained in culture for preservation of the protozoan. As far as the results of the fresh fecal examination were, 48/70 samples positive for Parabasalids, corresponding to 68.57% of the breeding females, the main clinical picture reported was dysentery in 31/48 (64.58%) in sows, p <0.05, and no cases of atrophic rhinitis were reported. Regarding the parasitological result, 72.7% of the matrices were positive for at least one parasite, and there was case of co-infection in 75% of the infected animals. In the matrices, coccidiosis was more frequent (60.87%), followed by Parabasalidea (52.17%), Strongyloidea (47.8%) and Balantidium (26.08%). It was concluded that Parabasal?deos are present in the herd and that they play a relevant role in the infection of animals in confinement with or without the diagnosis of dysentery.Esta pesquisa foi realizada no Laborat?rio de Suinocultura do Instituto Federal Rio de Janeiro (IFRJ), Campus Nilo Pe?anha, na microrregi?o do vale do Para?ba, mesorregi?o sul Fluminense, RJ , para monitoramento de Parabasal?deos, parasitos encontrados em matrizes su?nas, respons?veis por disenteria e rinite atr?fica, apesar da ?nfase na tecnologia e no manejo sanit?rio do rebanho estudado. O objetivo deste trabalho foi determinar e diagnosticar a presen?a de Parabasal?deos nas fezes de su?nos com e sem sintomatologia gastroent?rica e/ou respirat?ria, cuja principal via de transmiss?o seria a feco-oral. A metodologia proposta foi avaliar a presen?a deste agente etiol?gico nas matrizes do plantel de su?nos confinadas no UFRJ. Para tal, foram coletadas fezes da ampola retal, de 70 f?meas mantidas ? temperatura ambiente (?30oC) que, ao chegarem no Laborat?rio de Patologia de Reprodu??o da UFRRJ, foram homogeneizadas em solu??o fisiol?gica tamponada (PBS) na propor??o (1:10) de 5,0 gramas de fezes para 50ml de PBS, mantidas em estufa bacteriol?gica, a ? 37?C, durante 24h, a fim de identificar o protozo?rio. O diagn?stico com base na morfologia foi feito com base na observa??o de montagem a fresco ou atrav?s de esfrega?os corados em Pan?ptico R?pido com aux?lio de um microsc?pio ?tico binocular e com objetivas de 10 e 40X. As amostras positivas foram mantidas em cultura para preserva??o do protozo?rio. Quanto ao resultado do exame a fresco das fezes foi de 48/70 amostras positivas para Parabasal?deos, correspondendo a 68,57% das f?meas em reprodu??o, principal quadro clinico relatado foi disenteria em 31/48 (64,58%) em porcas, p<0,05, e n?o foi relatado nenhum caso de rinite atr?fica. Quanto ao resultado parasitol?gico 72.7% das matrizes foram positivas para pelo menos um parasito, e houve caso de co-infec??o em 75% dos animais infectados. Nas matrizes, coccidios foi mais frequente (60.87%), seguido de Parabasalidea (52.17%), Strongyloidea (47.8%) and Balantidium (26.08%). Concluiu-se que os Parabasal?deos s?o presentes no rebanho e que desempenham um papel relevante na infec??o dos animais em confinamento com ou sem o diagn?stico de desinteria.Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2022-05-03T17:36:01Z No. of bitstreams: 1 2019 - Nelson Oscaranha Gonsales da Costa.pdf: 697291 bytes, checksum: fd381fcf1ebb63534c424592bda15fc4 (MD5)Made available in DSpace on 2022-05-03T17:36:02Z (GMT). No. of bitstreams: 1 2019 - Nelson Oscaranha Gonsales da Costa.pdf: 697291 bytes, checksum: fd381fcf1ebb63534c424592bda15fc4 (MD5) Previous issue date: 2019-02-28CAPES - Coordena??o de Aperfei?oamento de Pessoal de N?vel Superiorapplication/pdfhttps://tede.ufrrj.br/retrieve/69093/2019%20-%20Nelson%20Oscaranha%20Gonsales%20da%20Costa.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Medicina Veterin?ria (Patologia e Ci?ncias Cl?nicas)UFRRJBrasilInstituto de Veterin?riaAMIN, A.; NEUBAUER, C.; LIEBHART, D.; GRABENSTEINER, E.; HESS, M. Axenization and optimization of in vitro growth of clonal cultures of Tetratrichomonas gallinarum and Trichomonas gallinae. Experimental Parasitology, v.124, p.202-208, 2010. APPEL, L.; MICKELSEN W. D.; THOMAS, M. H.; HARMON, W. M. A comparison of techniques used for the diagnosis of Tritrichomonas foetus infections in beef bulls. Agri-practice, v.14, p. 30-34, 1993. BENCHIMOL, M. Trichomonads under microscopy. 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dc.title.por.fl_str_mv Diagn?stico parasitol?gico com enfoque: nos parabasal?deos em matrizes su?nas de granja na microrregi?o do Vale do Paraiba fluminense, RJ
dc.title.alternative.eng.fl_str_mv Parasitological diagnosis with na approach: in parabasalides in swine of farm in the southern fluminense Paraiba Valley, RJ
title Diagn?stico parasitol?gico com enfoque: nos parabasal?deos em matrizes su?nas de granja na microrregi?o do Vale do Paraiba fluminense, RJ
spellingShingle Diagn?stico parasitol?gico com enfoque: nos parabasal?deos em matrizes su?nas de granja na microrregi?o do Vale do Paraiba fluminense, RJ
Costa, Nelson Oscaranha Gonsales da
parabasal?deos
fezes
porcas
disenteria
parabasalids
feces
sows
dysentery
Medicina Veterin?ria
title_short Diagn?stico parasitol?gico com enfoque: nos parabasal?deos em matrizes su?nas de granja na microrregi?o do Vale do Paraiba fluminense, RJ
title_full Diagn?stico parasitol?gico com enfoque: nos parabasal?deos em matrizes su?nas de granja na microrregi?o do Vale do Paraiba fluminense, RJ
title_fullStr Diagn?stico parasitol?gico com enfoque: nos parabasal?deos em matrizes su?nas de granja na microrregi?o do Vale do Paraiba fluminense, RJ
title_full_unstemmed Diagn?stico parasitol?gico com enfoque: nos parabasal?deos em matrizes su?nas de granja na microrregi?o do Vale do Paraiba fluminense, RJ
title_sort Diagn?stico parasitol?gico com enfoque: nos parabasal?deos em matrizes su?nas de granja na microrregi?o do Vale do Paraiba fluminense, RJ
author Costa, Nelson Oscaranha Gonsales da
author_facet Costa, Nelson Oscaranha Gonsales da
author_role author
dc.contributor.advisor1.fl_str_mv Jesus, Vera L?cia Teixeira de
dc.contributor.advisor1ID.fl_str_mv CPF: 708.346.107-44
dc.contributor.referee1.fl_str_mv Jesus, Vera L?cia Teixeira de
dc.contributor.referee2.fl_str_mv Lopes, Carlos Wilson Gomes
dc.contributor.referee3.fl_str_mv Cardozo, Sergian Vianna
dc.contributor.authorID.fl_str_mv CPF: 029.290.967-56
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/4223689467896974
dc.contributor.author.fl_str_mv Costa, Nelson Oscaranha Gonsales da
contributor_str_mv Jesus, Vera L?cia Teixeira de
Jesus, Vera L?cia Teixeira de
Lopes, Carlos Wilson Gomes
Cardozo, Sergian Vianna
dc.subject.por.fl_str_mv parabasal?deos
fezes
porcas
disenteria
topic parabasal?deos
fezes
porcas
disenteria
parabasalids
feces
sows
dysentery
Medicina Veterin?ria
dc.subject.eng.fl_str_mv parabasalids
feces
sows
dysentery
dc.subject.cnpq.fl_str_mv Medicina Veterin?ria
description This research was carried out in the Swine Laboratory of the Federal Institute of Rio de Janeiro (IFRJ), Nilo Pe?anha Campus, in the Para?ba valley microregion, southern Fluminense mesoregion, for the monitoring of Parabasalids, parasites found in swine matrices responsible for dysentery and atrophic rhinitis, despite the emphasis on technology and sanitary management of the herd studied. The objective of this work was to determine and diagnose the presence of Parabasal?deos in swine feces with and without gastrointestinal and /or respiratory symptoms, whose main route of transmission would be oral fecal. The proposed methodology was to evaluate the presence of this etiological agent in the pigs of the pigs confined in the UFRJ. For this, feces were collected from the rectal ampulla of 70 females kept at room temperature (? 30oC), which were homogenized in buffered physiological solution (PBS) at the ratio (1:10) at the Laboratory of Reproductive Pathology of the UFRRJ, , from 5.0 grams of faeces to 50 ml of PBS, kept in a bacteriological oven at ? 37 ? C for 24 hours in order to identify the protozoan. The diagnosis based on the morphology was made based on the observation of fresh assembly or through swabs stained in Quick Panoptico with the aid of a binocular optical microscope and with 10 and 40X objective. Positive samples were maintained in culture for preservation of the protozoan. As far as the results of the fresh fecal examination were, 48/70 samples positive for Parabasalids, corresponding to 68.57% of the breeding females, the main clinical picture reported was dysentery in 31/48 (64.58%) in sows, p <0.05, and no cases of atrophic rhinitis were reported. Regarding the parasitological result, 72.7% of the matrices were positive for at least one parasite, and there was case of co-infection in 75% of the infected animals. In the matrices, coccidiosis was more frequent (60.87%), followed by Parabasalidea (52.17%), Strongyloidea (47.8%) and Balantidium (26.08%). It was concluded that Parabasal?deos are present in the herd and that they play a relevant role in the infection of animals in confinement with or without the diagnosis of dysentery.
publishDate 2019
dc.date.issued.fl_str_mv 2019-02-28
dc.date.accessioned.fl_str_mv 2022-05-03T17:36:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv COSTA, Nelson Oscaranha Gonsales da. Diagn?stico parasitol?gico com enfoque: nos parabasal?deos em matrizes su?nas de granja na microrregi?o do Vale do Para?ba fluminense, RJ. 2019. 45 f. Disserta??o (Mesytrado em Medicina Veterin?ria, Patologia Animal) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2019.
dc.identifier.uri.fl_str_mv https://tede.ufrrj.br/jspui/handle/jspui/5597
identifier_str_mv COSTA, Nelson Oscaranha Gonsales da. Diagn?stico parasitol?gico com enfoque: nos parabasal?deos em matrizes su?nas de granja na microrregi?o do Vale do Para?ba fluminense, RJ. 2019. 45 f. Disserta??o (Mesytrado em Medicina Veterin?ria, Patologia Animal) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2019.
url https://tede.ufrrj.br/jspui/handle/jspui/5597
dc.language.iso.fl_str_mv por
language por
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