Avalia??o dos efeitos antinociceptivo e anti-inflamat?rio de extrato, fra??es e compostos de Borreria verticillata (L.) G. Mey (Vassourinha de bot?o)

Detalhes bibliográficos
Autor(a) principal: Mariano, Geysa de Matos Dutra
Data de Publicação: 2017
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
Texto Completo: https://tede.ufrrj.br/jspui/handle/jspui/4318
Resumo: Borreria verticillata (L.) G. Mey is a herbaceous plant of the family Rubiaceae, considered a weed and known as vassourinha-de-bot?o. Because it is used in the population as analgesic and antipyretic, the objective of this work was to evaluate the antinociceptive and anti-inflammatory potential of B. verticillata (EFBV) aqueous extract of the leaves, flavonoid (Flav), butanol (BuOH) and ethyl acetate (AcOEt) fractions and compounds rutin (Ru, 58% yield) and chlorogenic acid (AC, 3% yield), administered orally in mice. Previous studies have shown the activity of the EFBV extract, the flavonoid rutin and the phenolic compound chlorogenic acid in several biological activities, encouraging our research with these compounds, aiming to confirm the uses of B. verticillata. The evaluation of antinociceptive activity by the writhing test (acetic acid), showed that the administration of EFBV (100, 300 and 1000mg/kg) produced inhibitions in the number of writhes of 23.6%, 30.3% and 47.3%, respectively, when compared to the vehicle, as well as the positive control (CP) indomethacin reduced of the writhes. In the tail-immersion test, the administration of EFBV did not show changes in the latency time of the animals in front of the thermal stimulus and to confirm these antinociceptive results, we evaluated the motor activity of the EFBV, in which its administration did not modify the dwell time of the animals in the rota-rod. The evaluation of anti-inflammatory activity through the carrageenan-induced pleurisy test showed that EFBV (1g/kg) inhibited leukocyte migration in 33.7%, compared to the vehicle and the fractions Flav (20mg/kg), BuOH (200mg/kg), AcOEt (25mg/kg) presented reductions in the migration of 38.9%, 71.1%, 78,8% and 85.5% respectively. Evaluating the antiedematogenic activity with the ear edema test (croton oil), the administration of EFBV (1g/kg) reduced edema by 47.6%. In zymosan-induced articular arthritis, EFBV(1g/kg), Ru (30mg/kg) and AC (2mg/kg) reduced edema of the femoral-tibial joint in 57.8%, 49.2% and 51.7%, respectively, when compared to vehicles. In the same test, EFBV (1g/kg), Ru (30mg/kg) and AC (2mg/kg) compounds inhibited the leukocyte migration to the joint cavity in 64.9%, 79.5% and 77.8%, when compared to their respective vehicles and CP dexamethasone also produced reductions in the two parameters evaluated in this model. In the measurement of the femoral-tibial diameter, followed by cytokine TNF-? dosing in the joint macerate, EFBV, Ru and AC reduced joint thickness by 58.1% (EFBV, Ru) and 40.7% (AC), when compared to saline-zymosan control, with CP dexamethasone reduced the thickness by 76.5%. The concentration of TNF-? after administration of EFBV, Ru or AC, where we observed reductions of 30.4%, 37.0%, 21.7%, respectively, in the cytokine concentration, compared to saline-zymosan control and similar to CP, reducing this concentration by 50.0%. These results show that the antinociception of the EFBV, in the writhing test, participate in anti-inflammatory mechanisms, and this extract showed antiedematogenic activity (ear edema) and, together with the compounds Ru and AC, at the femoral-tibial joint in arthritis by zimosan. In addition, EFBV and fractions (Flav, BuOH and AcOEt) inhibited leukocyte migration in pleurisy by carrageenan, and the extract, Ru and AC also showed similar effectiveness in zymosan-induced arthritis, also reducing the concentration of TNF- ?. These results suggest that the anti-inflammatory, antinociceptive and anti-arthritis effects involved the inhibition of TNF-?, justifying the biological effects of EFBV, rutin and chlorogenic acid, and may explain some of the uses of B. verticillata (L.) G. Mey
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spelling Vanderlinde, Frederico Argollo49295896734http://lattes.cnpq.br/3183716703092107Vanderlinde, Frederico ArgolloMarinho, Bruno Guimar?esMatheus, Maria Eline10389316733http://lattes.cnpq.br/2615320530323647Mariano, Geysa de Matos Dutra2021-01-06T17:57:29Z2017-02-22MARIANO, Geysa de Matos Dutra. Avalia??o dos efeitos antinociceptivo e anti-inflamat?rio de extrato, fra??es e compostos de Borreria verticillata (L.) G. Mey (Vassourinha de bot?o). 2017. 73 f. Disserta??o (Mestrado em Ci?ncias Fisiol?gicas) - Instituto de Ci?ncias Biol?gicas e da Sa?de, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2017.https://tede.ufrrj.br/jspui/handle/jspui/4318Borreria verticillata (L.) G. Mey is a herbaceous plant of the family Rubiaceae, considered a weed and known as vassourinha-de-bot?o. Because it is used in the population as analgesic and antipyretic, the objective of this work was to evaluate the antinociceptive and anti-inflammatory potential of B. verticillata (EFBV) aqueous extract of the leaves, flavonoid (Flav), butanol (BuOH) and ethyl acetate (AcOEt) fractions and compounds rutin (Ru, 58% yield) and chlorogenic acid (AC, 3% yield), administered orally in mice. Previous studies have shown the activity of the EFBV extract, the flavonoid rutin and the phenolic compound chlorogenic acid in several biological activities, encouraging our research with these compounds, aiming to confirm the uses of B. verticillata. The evaluation of antinociceptive activity by the writhing test (acetic acid), showed that the administration of EFBV (100, 300 and 1000mg/kg) produced inhibitions in the number of writhes of 23.6%, 30.3% and 47.3%, respectively, when compared to the vehicle, as well as the positive control (CP) indomethacin reduced of the writhes. In the tail-immersion test, the administration of EFBV did not show changes in the latency time of the animals in front of the thermal stimulus and to confirm these antinociceptive results, we evaluated the motor activity of the EFBV, in which its administration did not modify the dwell time of the animals in the rota-rod. The evaluation of anti-inflammatory activity through the carrageenan-induced pleurisy test showed that EFBV (1g/kg) inhibited leukocyte migration in 33.7%, compared to the vehicle and the fractions Flav (20mg/kg), BuOH (200mg/kg), AcOEt (25mg/kg) presented reductions in the migration of 38.9%, 71.1%, 78,8% and 85.5% respectively. Evaluating the antiedematogenic activity with the ear edema test (croton oil), the administration of EFBV (1g/kg) reduced edema by 47.6%. In zymosan-induced articular arthritis, EFBV(1g/kg), Ru (30mg/kg) and AC (2mg/kg) reduced edema of the femoral-tibial joint in 57.8%, 49.2% and 51.7%, respectively, when compared to vehicles. In the same test, EFBV (1g/kg), Ru (30mg/kg) and AC (2mg/kg) compounds inhibited the leukocyte migration to the joint cavity in 64.9%, 79.5% and 77.8%, when compared to their respective vehicles and CP dexamethasone also produced reductions in the two parameters evaluated in this model. In the measurement of the femoral-tibial diameter, followed by cytokine TNF-? dosing in the joint macerate, EFBV, Ru and AC reduced joint thickness by 58.1% (EFBV, Ru) and 40.7% (AC), when compared to saline-zymosan control, with CP dexamethasone reduced the thickness by 76.5%. The concentration of TNF-? after administration of EFBV, Ru or AC, where we observed reductions of 30.4%, 37.0%, 21.7%, respectively, in the cytokine concentration, compared to saline-zymosan control and similar to CP, reducing this concentration by 50.0%. These results show that the antinociception of the EFBV, in the writhing test, participate in anti-inflammatory mechanisms, and this extract showed antiedematogenic activity (ear edema) and, together with the compounds Ru and AC, at the femoral-tibial joint in arthritis by zimosan. In addition, EFBV and fractions (Flav, BuOH and AcOEt) inhibited leukocyte migration in pleurisy by carrageenan, and the extract, Ru and AC also showed similar effectiveness in zymosan-induced arthritis, also reducing the concentration of TNF- ?. These results suggest that the anti-inflammatory, antinociceptive and anti-arthritis effects involved the inhibition of TNF-?, justifying the biological effects of EFBV, rutin and chlorogenic acid, and may explain some of the uses of B. verticillata (L.) G. MeyA Borreria verticillata (L.) G. Mey ? uma planta herb?cea da fam?lia Rubiaceae, considerada uma erva daninha e conhecida como vassourinha-de-bot?o. Por ser usada pela popula??o como analg?sica e antipir?tica, o objetivo deste trabalho foi avaliar o potencial antinociceptivo e anti-inflamat?rio do extrato aquoso das folhas de B. verticillata (EFBV), das fra??es flavono?dica (Flav), butan?lica (BuOH) e acetato de etila (AcOEt) e dos compostos rutina (Ru, rendimento de 58%) e ?cido clorog?nico (AC, rendimento de 3%), administrados via oral em camundongos. Estudos anteriores, mostraram a atividade do extrato de EFBV, do flavon?ide rutina e do composto fen?lico ?cido clorog?nico em diversas atividades biol?gicas, incentivando nossa pesquisa com estes compostos, objetivando a confirma??o dos usos da B. verticillata. A avalia??o da atividade antinociceptiva pelo modelo das contor??es abdominais (?cido ac?tico), mostrou que a administra??o de EFBV (100, 300 e 1000mg/kg) produziu inibi??es no n?mero de contor??es de 23,6%, 30,3% e 47,3% respectivamente, quando comparados ao ve?culo, assim como o controle positivo (CP) indometacina reduziu as contor??es. No modelo de imers?o cauda, a administra??o de EFBV n?o mostrou altera??es no tempo de lat?ncia dos animais diante do est?mulo t?rmico e para confirmar estes resultados antinociceptivos, avaliamos a atividade motora do EFBV, no qual sua administra??o n?o modificou o tempo de perman?ncia dos animais no rota-rod. A avalia??o da atividade anti-inflamat?ria, utilizando o modelo de pleurisia por carragenina, mostrou que a administra??o de EFBV (1g/kg) inibiu a migra??o leucocit?ria em 33,7%, comparativamente ao ve?culo e as fra??es Flav (20mg/kg), BuOH (200mg/kg) e AcOEt (25mg/kg) tamb?m apresentaram redu??es na migra??o leucocit?ria de 38,9%, 71,1%, 78,8% respectivamente. Avaliando a atividade antiedematog?nica com o modelo de edema de orelha (?leo de cr?ton), a administra??o de EFBV (1g/kg) reduziu o edema de orelha em 47,6%. Na artrite articular por zimosan, o EFBV (1g/kg), a Ru (30mg/kg) e o AC (2mg/kg) reduziram o edema da articula??o f?mur-tibial em 57,8%, 49,2% e 51,7% respectivamente, quando comparados aos ve?culos. Neste mesmo modelo, o EFBV (1g/kg), os compostos Ru (30mg/kg) e AC (2mg/kg) inibiram a migra??o leucocit?ria para cavidade articular em 64,9%, 79,5% e 77,8%, quando comparados aos seus respectivos ve?culos, assim como a CP dexametasona tamb?m produziu redu??es nos dois par?metros avaliados neste modelo. Na mensura??o do di?metro f?mur-tibial, seguida da dosagem da citocina TNF-? no macerado articular, EFBV, Ru e AC reduziram a espessura articular em 58,1% (EFBV, Ru) e 40,7% (AC), quando comparados ao controle salina-zimosam. Avaliamos a concentra??o de TNF-? ap?s administra??o de EFBV, Ru ou AC, onde observamos redu??es de 30,4%, 37,0%, 21,7%, respectivamente, na concentra??o da citocina, comparados com controle salina-zimosan e semelhante ao CP, reduzindo em 50,0% esta concentra??o. Estes resultados evidenciam que a antinocicep??o do EFBV, no modelo das contor??es abdominais mostrando envolvimento de mecanismos anti-inflamat?rios, tendo este extrato apresentado atividade antiedematog?nica no modelo de edema de orelha e, juntamente com os compostos Ru e AC, no modelo de artrite por zimosan. Adicionalmente, o EFBV e as fra??es (Flav, BuOH e AcOEt) inibiram a migra??o leucocit?ria na pleurisia por carragenina, tendo tamb?m o extrato, Ru e AC apresentado efetividade semelhante na artrite articular por zimosan, reduzindo tamb?m a concentra??o de TNF-?. Esses resultados sugerem que os efeitos anti-inflamat?rio, antinociceptivo e anti-artrite obtidos envolve a inibi??o do TNF-?, justificando os efeitos biol?gicos do EFBV, da rutina e do ?cido clorog?nico, podendo explicar alguns dos usos da B. verticillata (L.) G. Mey.Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2021-01-06T17:57:28Z No. of bitstreams: 1 2017 - Geysa de Matos Dutra Mariano.pdf: 2380734 bytes, checksum: 2a32fa608cc5446c25f43f1f515803c2 (MD5)Made available in DSpace on 2021-01-06T17:57:29Z (GMT). No. of bitstreams: 1 2017 - Geysa de Matos Dutra Mariano.pdf: 2380734 bytes, checksum: 2a32fa608cc5446c25f43f1f515803c2 (MD5) Previous issue date: 2017-02-22application/pdfhttps://tede.ufrrj.br/retrieve/63709/2017%20-%20Geysa%20de%20Matos%20Dutra%20Mariano.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Ci?ncias Fisiol?gicasUFRRJBrasilInstituto de Ci?ncias Biol?gicas e da Sa?deABDULLAHI-GERO, H. S., AHMED, A., HUSSAINI, I. M. Preliminary evaluation of ethanol leaf extract of Borreria verticillata Linn (Rubiaceae) for analgesic and anti-inflammatory effects. Journal of Medicinal Plants Research., Nigeria, v.8, n.20, p. 736-747, mai, 2014. 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Mey (Vassourinha de bot?o)Evaluation of antinociceptive and anti-inflammatory effects of extract, fractions and compounds of Borreria verticillata (L.) G. 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dc.title.por.fl_str_mv Avalia??o dos efeitos antinociceptivo e anti-inflamat?rio de extrato, fra??es e compostos de Borreria verticillata (L.) G. Mey (Vassourinha de bot?o)
dc.title.alternative.por.fl_str_mv Evaluation of antinociceptive and anti-inflammatory effects of extract, fractions and compounds of Borreria verticillata (L.) G. Mey (Vassourinha de bot?o)
title Avalia??o dos efeitos antinociceptivo e anti-inflamat?rio de extrato, fra??es e compostos de Borreria verticillata (L.) G. Mey (Vassourinha de bot?o)
spellingShingle Avalia??o dos efeitos antinociceptivo e anti-inflamat?rio de extrato, fra??es e compostos de Borreria verticillata (L.) G. Mey (Vassourinha de bot?o)
Mariano, Geysa de Matos Dutra
Antinociceptivo
Anti-inflamat?rio
Borreria verticillata (L.) G. Mey
Antinociceptive
Anti-inflammatory
Borreria verticillata
Fisiologia
title_short Avalia??o dos efeitos antinociceptivo e anti-inflamat?rio de extrato, fra??es e compostos de Borreria verticillata (L.) G. Mey (Vassourinha de bot?o)
title_full Avalia??o dos efeitos antinociceptivo e anti-inflamat?rio de extrato, fra??es e compostos de Borreria verticillata (L.) G. Mey (Vassourinha de bot?o)
title_fullStr Avalia??o dos efeitos antinociceptivo e anti-inflamat?rio de extrato, fra??es e compostos de Borreria verticillata (L.) G. Mey (Vassourinha de bot?o)
title_full_unstemmed Avalia??o dos efeitos antinociceptivo e anti-inflamat?rio de extrato, fra??es e compostos de Borreria verticillata (L.) G. Mey (Vassourinha de bot?o)
title_sort Avalia??o dos efeitos antinociceptivo e anti-inflamat?rio de extrato, fra??es e compostos de Borreria verticillata (L.) G. Mey (Vassourinha de bot?o)
author Mariano, Geysa de Matos Dutra
author_facet Mariano, Geysa de Matos Dutra
author_role author
dc.contributor.advisor1.fl_str_mv Vanderlinde, Frederico Argollo
dc.contributor.advisor1ID.fl_str_mv 49295896734
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/3183716703092107
dc.contributor.referee1.fl_str_mv Vanderlinde, Frederico Argollo
dc.contributor.referee2.fl_str_mv Marinho, Bruno Guimar?es
dc.contributor.referee3.fl_str_mv Matheus, Maria Eline
dc.contributor.authorID.fl_str_mv 10389316733
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/2615320530323647
dc.contributor.author.fl_str_mv Mariano, Geysa de Matos Dutra
contributor_str_mv Vanderlinde, Frederico Argollo
Vanderlinde, Frederico Argollo
Marinho, Bruno Guimar?es
Matheus, Maria Eline
dc.subject.por.fl_str_mv Antinociceptivo
Anti-inflamat?rio
Borreria verticillata (L.) G. Mey
topic Antinociceptivo
Anti-inflamat?rio
Borreria verticillata (L.) G. Mey
Antinociceptive
Anti-inflammatory
Borreria verticillata
Fisiologia
dc.subject.eng.fl_str_mv Antinociceptive
Anti-inflammatory
Borreria verticillata
dc.subject.cnpq.fl_str_mv Fisiologia
description Borreria verticillata (L.) G. Mey is a herbaceous plant of the family Rubiaceae, considered a weed and known as vassourinha-de-bot?o. Because it is used in the population as analgesic and antipyretic, the objective of this work was to evaluate the antinociceptive and anti-inflammatory potential of B. verticillata (EFBV) aqueous extract of the leaves, flavonoid (Flav), butanol (BuOH) and ethyl acetate (AcOEt) fractions and compounds rutin (Ru, 58% yield) and chlorogenic acid (AC, 3% yield), administered orally in mice. Previous studies have shown the activity of the EFBV extract, the flavonoid rutin and the phenolic compound chlorogenic acid in several biological activities, encouraging our research with these compounds, aiming to confirm the uses of B. verticillata. The evaluation of antinociceptive activity by the writhing test (acetic acid), showed that the administration of EFBV (100, 300 and 1000mg/kg) produced inhibitions in the number of writhes of 23.6%, 30.3% and 47.3%, respectively, when compared to the vehicle, as well as the positive control (CP) indomethacin reduced of the writhes. In the tail-immersion test, the administration of EFBV did not show changes in the latency time of the animals in front of the thermal stimulus and to confirm these antinociceptive results, we evaluated the motor activity of the EFBV, in which its administration did not modify the dwell time of the animals in the rota-rod. The evaluation of anti-inflammatory activity through the carrageenan-induced pleurisy test showed that EFBV (1g/kg) inhibited leukocyte migration in 33.7%, compared to the vehicle and the fractions Flav (20mg/kg), BuOH (200mg/kg), AcOEt (25mg/kg) presented reductions in the migration of 38.9%, 71.1%, 78,8% and 85.5% respectively. Evaluating the antiedematogenic activity with the ear edema test (croton oil), the administration of EFBV (1g/kg) reduced edema by 47.6%. In zymosan-induced articular arthritis, EFBV(1g/kg), Ru (30mg/kg) and AC (2mg/kg) reduced edema of the femoral-tibial joint in 57.8%, 49.2% and 51.7%, respectively, when compared to vehicles. In the same test, EFBV (1g/kg), Ru (30mg/kg) and AC (2mg/kg) compounds inhibited the leukocyte migration to the joint cavity in 64.9%, 79.5% and 77.8%, when compared to their respective vehicles and CP dexamethasone also produced reductions in the two parameters evaluated in this model. In the measurement of the femoral-tibial diameter, followed by cytokine TNF-? dosing in the joint macerate, EFBV, Ru and AC reduced joint thickness by 58.1% (EFBV, Ru) and 40.7% (AC), when compared to saline-zymosan control, with CP dexamethasone reduced the thickness by 76.5%. The concentration of TNF-? after administration of EFBV, Ru or AC, where we observed reductions of 30.4%, 37.0%, 21.7%, respectively, in the cytokine concentration, compared to saline-zymosan control and similar to CP, reducing this concentration by 50.0%. These results show that the antinociception of the EFBV, in the writhing test, participate in anti-inflammatory mechanisms, and this extract showed antiedematogenic activity (ear edema) and, together with the compounds Ru and AC, at the femoral-tibial joint in arthritis by zimosan. In addition, EFBV and fractions (Flav, BuOH and AcOEt) inhibited leukocyte migration in pleurisy by carrageenan, and the extract, Ru and AC also showed similar effectiveness in zymosan-induced arthritis, also reducing the concentration of TNF- ?. These results suggest that the anti-inflammatory, antinociceptive and anti-arthritis effects involved the inhibition of TNF-?, justifying the biological effects of EFBV, rutin and chlorogenic acid, and may explain some of the uses of B. verticillata (L.) G. Mey
publishDate 2017
dc.date.issued.fl_str_mv 2017-02-22
dc.date.accessioned.fl_str_mv 2021-01-06T17:57:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv MARIANO, Geysa de Matos Dutra. Avalia??o dos efeitos antinociceptivo e anti-inflamat?rio de extrato, fra??es e compostos de Borreria verticillata (L.) G. Mey (Vassourinha de bot?o). 2017. 73 f. Disserta??o (Mestrado em Ci?ncias Fisiol?gicas) - Instituto de Ci?ncias Biol?gicas e da Sa?de, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2017.
dc.identifier.uri.fl_str_mv https://tede.ufrrj.br/jspui/handle/jspui/4318
identifier_str_mv MARIANO, Geysa de Matos Dutra. Avalia??o dos efeitos antinociceptivo e anti-inflamat?rio de extrato, fra??es e compostos de Borreria verticillata (L.) G. Mey (Vassourinha de bot?o). 2017. 73 f. Disserta??o (Mestrado em Ci?ncias Fisiol?gicas) - Instituto de Ci?ncias Biol?gicas e da Sa?de, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2017.
url https://tede.ufrrj.br/jspui/handle/jspui/4318
dc.language.iso.fl_str_mv por
language por
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