Efeito da Azadiractina no desenvolvimento do Trypanossoma cruzi (Cone DM-28C) em Rhodnius prolixus STAL, 1859 (Hemiptera, Rreduviidae)

Detalhes bibliográficos
Autor(a) principal: Gonzalez, Marcelo Salabert
Data de Publicação: 1992
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
Texto Completo: https://rima.ufrrj.br/jspui/handle/20.500.14407/11711
Resumo: Neste trabalho, foram investigados os efeitos da azadiractina A no curso da infecção por Trypanosoma cruzi no intestino do triatomíneo vetor Rhodnius prolixus. No Rhodnius prolixus o desenvolvimento do clone Dm-28c do T. cruzi decresce de uma maneira dose dependente, e a ED50 deste efeito inibitório da azadiractina de 0.25 ug/ml de sangue. Usando este inseto, nós conduzimos experimentos de longo prazo demonstrando que a azadiractina (1.0 ug/ml de sangue) bloqueia o desenvolvimento do T. cruzi durante 120 dias após o tratamento com a droga. Similarmente, a eliminação do T. cruzi nas fezes e urina foi bloqueada por um período de 50 dias após a infecção dos insetos tratados com azadiractina. Larvas de 5º estádio de R. prolixus infectadas com T. cruzi realizam uma drástica inibição do desenvolvimento do tripanosomatídeo quando tratadas com azadiractina A. A discussão destes resultados enfoca a possibilidade de que a azadiractina deve atuar diretamente na fisiologia intestinal e/ou indiretamente através do sistema neurosecretório.
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spelling Gonzalez, Marcelo SalabertGarcia, Eloi de Souzahttp://lattes.cnpq.br/6564076531735310Moya Borja, Gonzalo EfraínGarcia, Elói de SouzaPenna, Patrícia de Azambuja2023-12-22T01:56:29Z2023-12-22T01:56:29Z1992-10-29GONZALES, Marcelo Solabert. Efeito da Azadiractina no desenvolvimento do Trypanossoma cruzi (CLONE DM-28C) em Rhodnius prolixus STAL, 1859 (Hemiptera, Reduviidae). 1992. 166 f. Dissertação (Mestrado em Ciências Veterinárias) - Instituto de Ciências Biológicas e da Saúde, Universidade Federal Rural do Rio de Janeiro, Seropédica-RJ, 1992.https://rima.ufrrj.br/jspui/handle/20.500.14407/11711Neste trabalho, foram investigados os efeitos da azadiractina A no curso da infecção por Trypanosoma cruzi no intestino do triatomíneo vetor Rhodnius prolixus. No Rhodnius prolixus o desenvolvimento do clone Dm-28c do T. cruzi decresce de uma maneira dose dependente, e a ED50 deste efeito inibitório da azadiractina de 0.25 ug/ml de sangue. Usando este inseto, nós conduzimos experimentos de longo prazo demonstrando que a azadiractina (1.0 ug/ml de sangue) bloqueia o desenvolvimento do T. cruzi durante 120 dias após o tratamento com a droga. Similarmente, a eliminação do T. cruzi nas fezes e urina foi bloqueada por um período de 50 dias após a infecção dos insetos tratados com azadiractina. Larvas de 5º estádio de R. prolixus infectadas com T. cruzi realizam uma drástica inibição do desenvolvimento do tripanosomatídeo quando tratadas com azadiractina A. A discussão destes resultados enfoca a possibilidade de que a azadiractina deve atuar diretamente na fisiologia intestinal e/ou indiretamente através do sistema neurosecretório.Coordenação e Aperfeiçoamento de Pessoal de Nível Superior - CAPESOMSStudies were carried out the effects of azadirachtin on the course of Trypanosoma cruzi infection in the gut of triatomine vector, Rhodnius prolixus. In Rhodnius prolixus the development of T. cruzi clone Dm-28c decreased in a dose dependent manner, and ED50 of this inhibitory effect was 0.25 ug azadirachtin/ml bloodmeal. Using this insect, we conducted a long term experiment which showed that azadirachtin (1.0 ug/ml bloodmeal) completely blocks the development of T. cruzi even 120 days after treatment with the drug, and after four infectant meals. Similarly, the elimination of T. cruzi in feces and urine was also completely blocked over a period of 50 days after infection in insects treated with azadirachtin. Fifth-instar larvae of Rhodnius prolixus infected with T. cruzi displayed drastic inhibition of trypanosome development when treated with azadirachtin. 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ZELEDÓN, R., 1987. life cycle of Trypanosoma cruzi in the in the insect vector. In: Chagas Disease Vectors (Anatomic and Physiological Aspects). BRENNER, R. R. & STOKA, A. M., eds. 2: 59-75. ZELEDÓN, R., BOLANOS, R. & ROJAS, M., 1984. Scanning electron microscopy of the final phase c f the life cycle of T. cruzi in the insect vector. Acta. Trop., 41: 39-43.https://tede.ufrrj.br/retrieve/62171/1992%20-%20Marcelo%20Salabert%20Gonzalez.pdf.jpghttps://tede.ufrrj.br/jspui/handle/jspui/3944Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2020-09-28T15:02:25Z No. of bitstreams: 1 1992 - Marcelo Salabert Gonzalez.pdf: 749486 bytes, checksum: 68b6a62af438043d8e57418564b19607 (MD5)Made available in DSpace on 2020-09-28T15:02:25Z (GMT). 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dc.title.por.fl_str_mv Efeito da Azadiractina no desenvolvimento do Trypanossoma cruzi (Cone DM-28C) em Rhodnius prolixus STAL, 1859 (Hemiptera, Rreduviidae)
title Efeito da Azadiractina no desenvolvimento do Trypanossoma cruzi (Cone DM-28C) em Rhodnius prolixus STAL, 1859 (Hemiptera, Rreduviidae)
spellingShingle Efeito da Azadiractina no desenvolvimento do Trypanossoma cruzi (Cone DM-28C) em Rhodnius prolixus STAL, 1859 (Hemiptera, Rreduviidae)
Gonzalez, Marcelo Salabert
Medicina Veterinária
Medicina Veterinária
title_short Efeito da Azadiractina no desenvolvimento do Trypanossoma cruzi (Cone DM-28C) em Rhodnius prolixus STAL, 1859 (Hemiptera, Rreduviidae)
title_full Efeito da Azadiractina no desenvolvimento do Trypanossoma cruzi (Cone DM-28C) em Rhodnius prolixus STAL, 1859 (Hemiptera, Rreduviidae)
title_fullStr Efeito da Azadiractina no desenvolvimento do Trypanossoma cruzi (Cone DM-28C) em Rhodnius prolixus STAL, 1859 (Hemiptera, Rreduviidae)
title_full_unstemmed Efeito da Azadiractina no desenvolvimento do Trypanossoma cruzi (Cone DM-28C) em Rhodnius prolixus STAL, 1859 (Hemiptera, Rreduviidae)
title_sort Efeito da Azadiractina no desenvolvimento do Trypanossoma cruzi (Cone DM-28C) em Rhodnius prolixus STAL, 1859 (Hemiptera, Rreduviidae)
author Gonzalez, Marcelo Salabert
author_facet Gonzalez, Marcelo Salabert
author_role author
dc.contributor.author.fl_str_mv Gonzalez, Marcelo Salabert
dc.contributor.advisor1.fl_str_mv Garcia, Eloi de Souza
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/6564076531735310
dc.contributor.referee1.fl_str_mv Moya Borja, Gonzalo Efraín
dc.contributor.referee2.fl_str_mv Garcia, Elói de Souza
dc.contributor.referee3.fl_str_mv Penna, Patrícia de Azambuja
contributor_str_mv Garcia, Eloi de Souza
Moya Borja, Gonzalo Efraín
Garcia, Elói de Souza
Penna, Patrícia de Azambuja
dc.subject.por.fl_str_mv Medicina Veterinária
topic Medicina Veterinária
Medicina Veterinária
dc.subject.cnpq.fl_str_mv Medicina Veterinária
description Neste trabalho, foram investigados os efeitos da azadiractina A no curso da infecção por Trypanosoma cruzi no intestino do triatomíneo vetor Rhodnius prolixus. No Rhodnius prolixus o desenvolvimento do clone Dm-28c do T. cruzi decresce de uma maneira dose dependente, e a ED50 deste efeito inibitório da azadiractina de 0.25 ug/ml de sangue. Usando este inseto, nós conduzimos experimentos de longo prazo demonstrando que a azadiractina (1.0 ug/ml de sangue) bloqueia o desenvolvimento do T. cruzi durante 120 dias após o tratamento com a droga. Similarmente, a eliminação do T. cruzi nas fezes e urina foi bloqueada por um período de 50 dias após a infecção dos insetos tratados com azadiractina. Larvas de 5º estádio de R. prolixus infectadas com T. cruzi realizam uma drástica inibição do desenvolvimento do tripanosomatídeo quando tratadas com azadiractina A. A discussão destes resultados enfoca a possibilidade de que a azadiractina deve atuar diretamente na fisiologia intestinal e/ou indiretamente através do sistema neurosecretório.
publishDate 1992
dc.date.issued.fl_str_mv 1992-10-29
dc.date.accessioned.fl_str_mv 2023-12-22T01:56:29Z
dc.date.available.fl_str_mv 2023-12-22T01:56:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv GONZALES, Marcelo Solabert. Efeito da Azadiractina no desenvolvimento do Trypanossoma cruzi (CLONE DM-28C) em Rhodnius prolixus STAL, 1859 (Hemiptera, Reduviidae). 1992. 166 f. Dissertação (Mestrado em Ciências Veterinárias) - Instituto de Ciências Biológicas e da Saúde, Universidade Federal Rural do Rio de Janeiro, Seropédica-RJ, 1992.
dc.identifier.uri.fl_str_mv https://rima.ufrrj.br/jspui/handle/20.500.14407/11711
identifier_str_mv GONZALES, Marcelo Solabert. Efeito da Azadiractina no desenvolvimento do Trypanossoma cruzi (CLONE DM-28C) em Rhodnius prolixus STAL, 1859 (Hemiptera, Reduviidae). 1992. 166 f. Dissertação (Mestrado em Ciências Veterinárias) - Instituto de Ciências Biológicas e da Saúde, Universidade Federal Rural do Rio de Janeiro, Seropédica-RJ, 1992.
url https://rima.ufrrj.br/jspui/handle/20.500.14407/11711
dc.language.iso.fl_str_mv por
language por
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