Dietas, prebiótico e probiótico e seus efeitos sobre o microbioma intestinal de equinos

Detalhes bibliográficos
Autor(a) principal: Franzan, Bruna Caroline
Data de Publicação: 2021
Tipo de documento: Tese
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
Texto Completo: https://rima.ufrrj.br/jspui/handle/20.500.14407/9242
Resumo: A microbiota intestinal tem importância fundamental na nutrição e na saúde dos equinos e é influenciada por diversos fatores. Esse trabalho teve como objetivo avaliar uma dieta completa extrusada (CED) na alimentação de equinos e os fatores dietéticos que influenciam o microbioma fecal. Os equinos alimentados com a CED tiveram menor consumo de água e fibra, maior consumo de matéria seca (MS), extrato etéreo e energia bruta, menor produção e pH fecal. A digestibilidade da MS, matéria orgânica, extrato etéreo, proteína bruta e energia bruta foram superiores na CED. O tempo médio de retenção da digesta (TMR) da CED foi menor quando comparado a dieta de feno de Coastcross (CHD) quando estimado pelo marcador LIPE®. A abundância relativa do filo Actinobacteria foi maior após 28 dias de consumo de CHD comparado a 7 dias de consumo. A adaptação gradual de equinos ao consumo de CED afetou os filos Verrucomicrobia, Synergistetes, Tenericutes e Lentisphaerae. A inclusão de 30% de CED na dieta afeta a abundância de grupos bacterianos relacionados a atividade fermentativa, entretanto, a diversidade de espécies bacterianas das fezes foi mantida até o momento de consumo de 60% CED e 40% de CHD. A troca abrupta do CHD para CED resultou em redução do pH fecal 96 horas após a troca e redução da diversidade de espécies bacterianas 24 horas após a troca. Além disso, afetou os filos Bacteroidetes, Firmicutes, Verrucomicrobia, Proteobacteria, Elusimicrobia e Actinobacteria. A troca abrupta da CED para CHD resultou em redução do pH fecal em 24 horas após a troca e aumento da diversidade de espécies bacterianas 96 horas após a troca. Além disso, afetou os filos Synergistetes e Lentisphaerae. Por fim, ambas as trocas de dietas resultaram no aumento da abundância relativa de OTUs classificadas como Bacteroidetes e Treponema 24 horas após a troca. A suplementação com prebiótico inulina (PRE), probiótico Saccharomyces cerevisiae (PRO) e simbiótico inulina + Saccharomyces cerevisiae (SIM) não influenciou os índices de diversidade bacteriana observadas nas fezes de potros no período de desmame. A suplementação com PRE aumentou a classe Bacilli nas fezes dos potros lactentes em comparação ao grupo controle. O pH fecal dos potros suplementados com PRE foi superior ao grupo controle. A suplementação com PRO e SIM aumentou as classes Erysipelotrichia e Saccharimonadia, respectivamente, após três dias de consumo, entretanto, o efeito não foi prolongado. Além disso, houve diferença dos microbiomas fecais entre os sexos dos potros. Portanto, conclui-se que a CED pode ser usada na alimentação de equinos, desde que seja feito adaptação gradual. A composição da dieta e a suplementação com aditivos afetam o microbioma fecal de equinos e sua resposta a mudanças dietéticas.
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spelling Franzan, Bruna CarolineSilva, Vinicius Pimentel297.639.108-46Almeida, Fernando Queiroz deSilva, Vinicius PimentelManso Filho, CordeiroCosta, Márcio CarvalhoCoelho, Irene da SilvaAlmeida, Maria Izabel Vieira de106.175.936-98https://orcid.org/0000-0002-3290-6304http://lattes.cnpq.br/80125047394406382023-12-21T18:36:49Z2023-12-21T18:36:49Z2021-02-26FRANZAN, Bruna Caroline. Dietas, prebiótico e probiótico e seus efeitos sobre o microbioma intestinal de equinos. 2021. 130 f. Tese (Doutorado em Ciência Animal) - Instituto de Zootecnia, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, 2021.https://rima.ufrrj.br/jspui/handle/20.500.14407/9242A microbiota intestinal tem importância fundamental na nutrição e na saúde dos equinos e é influenciada por diversos fatores. Esse trabalho teve como objetivo avaliar uma dieta completa extrusada (CED) na alimentação de equinos e os fatores dietéticos que influenciam o microbioma fecal. Os equinos alimentados com a CED tiveram menor consumo de água e fibra, maior consumo de matéria seca (MS), extrato etéreo e energia bruta, menor produção e pH fecal. A digestibilidade da MS, matéria orgânica, extrato etéreo, proteína bruta e energia bruta foram superiores na CED. O tempo médio de retenção da digesta (TMR) da CED foi menor quando comparado a dieta de feno de Coastcross (CHD) quando estimado pelo marcador LIPE®. A abundância relativa do filo Actinobacteria foi maior após 28 dias de consumo de CHD comparado a 7 dias de consumo. A adaptação gradual de equinos ao consumo de CED afetou os filos Verrucomicrobia, Synergistetes, Tenericutes e Lentisphaerae. A inclusão de 30% de CED na dieta afeta a abundância de grupos bacterianos relacionados a atividade fermentativa, entretanto, a diversidade de espécies bacterianas das fezes foi mantida até o momento de consumo de 60% CED e 40% de CHD. A troca abrupta do CHD para CED resultou em redução do pH fecal 96 horas após a troca e redução da diversidade de espécies bacterianas 24 horas após a troca. Além disso, afetou os filos Bacteroidetes, Firmicutes, Verrucomicrobia, Proteobacteria, Elusimicrobia e Actinobacteria. A troca abrupta da CED para CHD resultou em redução do pH fecal em 24 horas após a troca e aumento da diversidade de espécies bacterianas 96 horas após a troca. Além disso, afetou os filos Synergistetes e Lentisphaerae. Por fim, ambas as trocas de dietas resultaram no aumento da abundância relativa de OTUs classificadas como Bacteroidetes e Treponema 24 horas após a troca. A suplementação com prebiótico inulina (PRE), probiótico Saccharomyces cerevisiae (PRO) e simbiótico inulina + Saccharomyces cerevisiae (SIM) não influenciou os índices de diversidade bacteriana observadas nas fezes de potros no período de desmame. A suplementação com PRE aumentou a classe Bacilli nas fezes dos potros lactentes em comparação ao grupo controle. O pH fecal dos potros suplementados com PRE foi superior ao grupo controle. A suplementação com PRO e SIM aumentou as classes Erysipelotrichia e Saccharimonadia, respectivamente, após três dias de consumo, entretanto, o efeito não foi prolongado. Além disso, houve diferença dos microbiomas fecais entre os sexos dos potros. Portanto, conclui-se que a CED pode ser usada na alimentação de equinos, desde que seja feito adaptação gradual. A composição da dieta e a suplementação com aditivos afetam o microbioma fecal de equinos e sua resposta a mudanças dietéticas.CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorThe intestinal microbiota plays important role in horse’s nutrition and health. This work aimed to evaluate a complete extruded diet (CED) to fed horses and the dietary factors that influence the faecal microbiome. Horses fed a CED had lower water and fiber intakes, higher dry matter (DM), ether extract and gross energy intakes, and lower production and fecal pH. The DM, organic matter, ether extract, crude protein and crude energy digestibility were superior in the CED. The CED mean retention time (MRT) was shorter compared to the Coastcross hay diet (CHD), when obtained by LIPE® marker. CHD intake for 21 days increased the abundance of Actinobacteria. The gradual adaptation of horses to the CED intake affected the phylum Verrucomicrobia, Synergistetes, Tenericutes and Lentisphaerae. The inclusion of 30% CED in the diet affects the abundance of bacterial groups in the fermentative activity, however, the diversity of bacterial species in the feces was maintained until the moment of 60% CED and 40% CHD intakes. The abrupt diet change from CHD to CED resulted in a reduction in faecal pH 96 hours after change and a bacterial diversity reduction 24 hours after chance. In addition, it affected the phyla Bacteroidetes, Firmicutes, Verrucomicrobia, Proteobacteria, Elusimicrobia and Actinobacteria. The abrupt change from CED to CHD resulted in a reduction in fecal pH 24 hours after the change and a bacterial species diversity increases of 96 hours after change. In addition, it affected the phyla Synergistetes and Lentisphaerae. Finally, both diet changes resulted in an increase in the abundance of OTUs classified as Bacteroidetes and Treponema 24 hours after change. Supplementation with prebiotic inulin (PRE), probiotic Saccharomyces cerevisiae (PRO) and symbiotic inulin + S. cerevisiae (SIM) did not influence the bacterial diversity species observed in the feces of foals during weaning. The supplementation with PRE increased the Bacilli class in the feces of suckling foals. The fecal pH of foals supplemented with PRE was higher than the control group. PRO and SIM supplementation increased the classes Erysipelotrichia and Saccharimonadia, respectively, after three days, however, the effect was not prolonged. In addition, there was a difference in fecal microbiomes between the sexes of foals. Therefore, it is concluded that CED can be used to feed horses, as long as a gradual adaptation is made. The composition of the diet and additives supplementation affect the fecal microbiome of horses and their response to dietary changes.application/pdfporUniversidade Federal Rural do Rio de JaneiroPrograma de Pós-Graduação em Ciência AnimalUFRRJBrasilInstituto de Zootecniadieta completafermentaçãofibramicrobiotasimbióticocomplete dietfermentationfibermicrobiotasimbioticZootecniaDietas, prebiótico e probiótico e seus efeitos sobre o microbioma intestinal de equinosDiets, prebiotic and probiotic in the intestinal microbiome of equinesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisAL JASSIM, R. A. M.; ANDREWS, F. M. 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Colonization of the stratified squamous epithelium of the nonsecreting area of horse stomach by lactobacilli. Applied and environmental microbiology, v. 66, n. 11, p. 5030-5034, 2000.https://tede.ufrrj.br/retrieve/70233/2021%20-%20Bruna%20Caroline%20Franzan.pdf.jpghttps://tede.ufrrj.br/jspui/handle/jspui/5866Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2022-08-11T19:29:04Z No. of bitstreams: 1 2021 - Bruna Caroline Franzan.pdf: 2257244 bytes, checksum: a920ba2a07dfea355da74b7f4721b495 (MD5)Made available in DSpace on 2022-08-11T19:29:04Z (GMT). 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dc.title.por.fl_str_mv Dietas, prebiótico e probiótico e seus efeitos sobre o microbioma intestinal de equinos
dc.title.alternative.eng.fl_str_mv Diets, prebiotic and probiotic in the intestinal microbiome of equines
title Dietas, prebiótico e probiótico e seus efeitos sobre o microbioma intestinal de equinos
spellingShingle Dietas, prebiótico e probiótico e seus efeitos sobre o microbioma intestinal de equinos
Franzan, Bruna Caroline
dieta completa
fermentação
fibra
microbiota
simbiótico
complete diet
fermentation
fiber
microbiota
simbiotic
Zootecnia
title_short Dietas, prebiótico e probiótico e seus efeitos sobre o microbioma intestinal de equinos
title_full Dietas, prebiótico e probiótico e seus efeitos sobre o microbioma intestinal de equinos
title_fullStr Dietas, prebiótico e probiótico e seus efeitos sobre o microbioma intestinal de equinos
title_full_unstemmed Dietas, prebiótico e probiótico e seus efeitos sobre o microbioma intestinal de equinos
title_sort Dietas, prebiótico e probiótico e seus efeitos sobre o microbioma intestinal de equinos
author Franzan, Bruna Caroline
author_facet Franzan, Bruna Caroline
author_role author
dc.contributor.author.fl_str_mv Franzan, Bruna Caroline
dc.contributor.advisor1.fl_str_mv Silva, Vinicius Pimentel
dc.contributor.advisor1ID.fl_str_mv 297.639.108-46
dc.contributor.advisor-co1.fl_str_mv Almeida, Fernando Queiroz de
dc.contributor.referee1.fl_str_mv Silva, Vinicius Pimentel
dc.contributor.referee2.fl_str_mv Manso Filho, Cordeiro
dc.contributor.referee3.fl_str_mv Costa, Márcio Carvalho
dc.contributor.referee4.fl_str_mv Coelho, Irene da Silva
dc.contributor.referee5.fl_str_mv Almeida, Maria Izabel Vieira de
dc.contributor.authorID.fl_str_mv 106.175.936-98
https://orcid.org/0000-0002-3290-6304
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/8012504739440638
contributor_str_mv Silva, Vinicius Pimentel
Almeida, Fernando Queiroz de
Silva, Vinicius Pimentel
Manso Filho, Cordeiro
Costa, Márcio Carvalho
Coelho, Irene da Silva
Almeida, Maria Izabel Vieira de
dc.subject.por.fl_str_mv dieta completa
fermentação
fibra
microbiota
simbiótico
topic dieta completa
fermentação
fibra
microbiota
simbiótico
complete diet
fermentation
fiber
microbiota
simbiotic
Zootecnia
dc.subject.eng.fl_str_mv complete diet
fermentation
fiber
microbiota
simbiotic
dc.subject.cnpq.fl_str_mv Zootecnia
description A microbiota intestinal tem importância fundamental na nutrição e na saúde dos equinos e é influenciada por diversos fatores. Esse trabalho teve como objetivo avaliar uma dieta completa extrusada (CED) na alimentação de equinos e os fatores dietéticos que influenciam o microbioma fecal. Os equinos alimentados com a CED tiveram menor consumo de água e fibra, maior consumo de matéria seca (MS), extrato etéreo e energia bruta, menor produção e pH fecal. A digestibilidade da MS, matéria orgânica, extrato etéreo, proteína bruta e energia bruta foram superiores na CED. O tempo médio de retenção da digesta (TMR) da CED foi menor quando comparado a dieta de feno de Coastcross (CHD) quando estimado pelo marcador LIPE®. A abundância relativa do filo Actinobacteria foi maior após 28 dias de consumo de CHD comparado a 7 dias de consumo. A adaptação gradual de equinos ao consumo de CED afetou os filos Verrucomicrobia, Synergistetes, Tenericutes e Lentisphaerae. A inclusão de 30% de CED na dieta afeta a abundância de grupos bacterianos relacionados a atividade fermentativa, entretanto, a diversidade de espécies bacterianas das fezes foi mantida até o momento de consumo de 60% CED e 40% de CHD. A troca abrupta do CHD para CED resultou em redução do pH fecal 96 horas após a troca e redução da diversidade de espécies bacterianas 24 horas após a troca. Além disso, afetou os filos Bacteroidetes, Firmicutes, Verrucomicrobia, Proteobacteria, Elusimicrobia e Actinobacteria. A troca abrupta da CED para CHD resultou em redução do pH fecal em 24 horas após a troca e aumento da diversidade de espécies bacterianas 96 horas após a troca. Além disso, afetou os filos Synergistetes e Lentisphaerae. Por fim, ambas as trocas de dietas resultaram no aumento da abundância relativa de OTUs classificadas como Bacteroidetes e Treponema 24 horas após a troca. A suplementação com prebiótico inulina (PRE), probiótico Saccharomyces cerevisiae (PRO) e simbiótico inulina + Saccharomyces cerevisiae (SIM) não influenciou os índices de diversidade bacteriana observadas nas fezes de potros no período de desmame. A suplementação com PRE aumentou a classe Bacilli nas fezes dos potros lactentes em comparação ao grupo controle. O pH fecal dos potros suplementados com PRE foi superior ao grupo controle. A suplementação com PRO e SIM aumentou as classes Erysipelotrichia e Saccharimonadia, respectivamente, após três dias de consumo, entretanto, o efeito não foi prolongado. Além disso, houve diferença dos microbiomas fecais entre os sexos dos potros. Portanto, conclui-se que a CED pode ser usada na alimentação de equinos, desde que seja feito adaptação gradual. A composição da dieta e a suplementação com aditivos afetam o microbioma fecal de equinos e sua resposta a mudanças dietéticas.
publishDate 2021
dc.date.issued.fl_str_mv 2021-02-26
dc.date.accessioned.fl_str_mv 2023-12-21T18:36:49Z
dc.date.available.fl_str_mv 2023-12-21T18:36:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv FRANZAN, Bruna Caroline. Dietas, prebiótico e probiótico e seus efeitos sobre o microbioma intestinal de equinos. 2021. 130 f. Tese (Doutorado em Ciência Animal) - Instituto de Zootecnia, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, 2021.
dc.identifier.uri.fl_str_mv https://rima.ufrrj.br/jspui/handle/20.500.14407/9242
identifier_str_mv FRANZAN, Bruna Caroline. Dietas, prebiótico e probiótico e seus efeitos sobre o microbioma intestinal de equinos. 2021. 130 f. Tese (Doutorado em Ciência Animal) - Instituto de Zootecnia, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, 2021.
url https://rima.ufrrj.br/jspui/handle/20.500.14407/9242
dc.language.iso.fl_str_mv por
language por
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