Estudo do potencial antioxidante de diferentes classes de moléculas isoladas de mel e própolis em células de Saccharomyces cerevisiae

Detalhes bibliográficos
Autor(a) principal: Prudêncio, Edlene Ribeiro
Data de Publicação: 2016
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
Texto Completo: https://rima.ufrrj.br/jspui/handle/20.500.14407/14648
Resumo: O mel e a própolis são produzidos por abelhas, a partir da coleta de néctar e seiva das plantas. No processo, os compostos fenólicos oriundos do metabolismo secundário dos vegetais são incorporados aos produtos mencionados. Amostras de mel e própolis de diferentes regiões foram caracterizadas e o conteúdo de compostos fenólicos pode ser dividido em: derivados do ácido hidroxibenzoico, derivados do ácido hidroxicinâmico, flavonoides e flavonoides glicosilados. Na literatura, valores de atividade antioxidante in vitro descritos divergem consideravelmente entre os autores. Além disso, os métodos in vitro (DPPH, ABTS, FRAPP, dentre outros) não representam condições fisiológicas como concentração de substratos e interação de metabólitos. A levedura Saccharomyces cerevisie é uma ferramenta para ensaio biológico, uma vez que apresenta elevada semelhança com células de mamíferos superiores no sistema de defesa antioxidante. O objetivo deste trabalho foi comparar o potencial antioxidante de diferentes classes de compostos fenólicos, utilizando os representantes morina, rutina, ácido siríngico e clorogênico em cepas controle (BY4741) e mutantes (sod1 e gsh1) de S. cerevisiae. Foram avaliados toxidez dose-dependente, tolerância ao estresse e peroxidação lipídica. Todos os compostos fenólicos testados foram efetivos em reduzir danos oxidativos intracelulares, com destaque para o ácido clorogênico na cepa controle. Quando comparado às células estressadas, este promoveu aumentos de 75% de sobrevivência, contra 57% em média dos demais tratamentos; e diminuição de 60% em níveis de peroxidação lipídica, contra redução próxima a 47% dos demais tratamentos. Nas cepas mutantes, todas as substâncias tiveram resultados semelhantes entre si. Desta forma, outros dois componentes da classe do ácido hidroxicinâmico, ácido cafeico e éster fenetílico do ácido cafeico (CAPE), foram testados comparativamente. Ambos atuaram como antioxidante em S. cerevisiae, entretanto CAPE foi a substância mais tóxica e também a que promoveu aumento mais significativo de glutationa reduzida dentre os derivados hidroxicinâmicos. Esse resultado corrobora com dados de estudos que apontam que a atividade protetora dos compostos fenólicos está relacionada a ativação do sistema antioxidante por ação xenobiótica dessas substâncias
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spelling Prudêncio, Edlene RibeiroRiger, Cristiano Jorge3009627700http://lattes.cnpq.br/8756160468801705Salles, Cristiane Martins Cardoso de3539928790http://lattes.cnpq.br/3610279707231709Barbosa, Maria Ivone Martins JacinthoPereira, Marcos Dias133.663.677-76http://lattes.cnpq.br/40730013458840452023-12-22T03:03:58Z2023-12-22T03:03:58Z2016-08-04PRUDÊNCIO, Edlene Ribeiro. Estudo do potencial antioxidante de diferentes classes de moléculas isoladas de mel e própolis em células de Saccharomyces cerevisiae. 2016. 74 f. Dissertação (Mestrado em Química) - Instituto de Ciências Exatas, Universidade Federal Rural do Rio de Janeiro, Seropédica - RJ, 2016.https://rima.ufrrj.br/jspui/handle/20.500.14407/14648O mel e a própolis são produzidos por abelhas, a partir da coleta de néctar e seiva das plantas. No processo, os compostos fenólicos oriundos do metabolismo secundário dos vegetais são incorporados aos produtos mencionados. Amostras de mel e própolis de diferentes regiões foram caracterizadas e o conteúdo de compostos fenólicos pode ser dividido em: derivados do ácido hidroxibenzoico, derivados do ácido hidroxicinâmico, flavonoides e flavonoides glicosilados. Na literatura, valores de atividade antioxidante in vitro descritos divergem consideravelmente entre os autores. Além disso, os métodos in vitro (DPPH, ABTS, FRAPP, dentre outros) não representam condições fisiológicas como concentração de substratos e interação de metabólitos. A levedura Saccharomyces cerevisie é uma ferramenta para ensaio biológico, uma vez que apresenta elevada semelhança com células de mamíferos superiores no sistema de defesa antioxidante. O objetivo deste trabalho foi comparar o potencial antioxidante de diferentes classes de compostos fenólicos, utilizando os representantes morina, rutina, ácido siríngico e clorogênico em cepas controle (BY4741) e mutantes (sod1 e gsh1) de S. cerevisiae. Foram avaliados toxidez dose-dependente, tolerância ao estresse e peroxidação lipídica. Todos os compostos fenólicos testados foram efetivos em reduzir danos oxidativos intracelulares, com destaque para o ácido clorogênico na cepa controle. Quando comparado às células estressadas, este promoveu aumentos de 75% de sobrevivência, contra 57% em média dos demais tratamentos; e diminuição de 60% em níveis de peroxidação lipídica, contra redução próxima a 47% dos demais tratamentos. Nas cepas mutantes, todas as substâncias tiveram resultados semelhantes entre si. Desta forma, outros dois componentes da classe do ácido hidroxicinâmico, ácido cafeico e éster fenetílico do ácido cafeico (CAPE), foram testados comparativamente. Ambos atuaram como antioxidante em S. cerevisiae, entretanto CAPE foi a substância mais tóxica e também a que promoveu aumento mais significativo de glutationa reduzida dentre os derivados hidroxicinâmicos. Esse resultado corrobora com dados de estudos que apontam que a atividade protetora dos compostos fenólicos está relacionada a ativação do sistema antioxidante por ação xenobiótica dessas substânciasHoney and propolis are produced by bees from the nectar and sap collected from plants. In the process, the phenolic compounds derived from secondary metabolism of plants are incorporated into the products mentioned. Honey and propolis samples from multiple regions have been characterized and phenolic compounds can be divided into: hydroxybenzoic acid derivatives, hydroxycinnamic acid derivatives, flavonoids and glycosylated flavonoids. In the literature, there is no consensus among the authors about the values for in vitro antioxidant activity. Moreover, these methods do not represent physiological conditions such as concentration of substrates and metabolites interaction. The yeast Saccharomyces cerevisiae is an alternative tool for a biological assays, since it is similar to mammalian cells. The main goal of this study is to compare the antioxidant potential of different phenolic compounds classes using representatives morin, rutin, chlorogenic acid and syringic acid in control strains (BY4741) and mutant (sod1 and gsh1) of S. cerevisiae. They were evaluated in dose-dependent toxicity, stress tolerance, and lipid peroxidation. All tested phenolic compounds were effective in reducing intracellular oxidative damage, especially the chlorogenic acid in the control strain. When compared to stressed cells, it promoted 75% increase on cell survival rates, compared with 57% on average for the other treatments; and 60% decrease in levels of lipid peroxidation, compared to reductions close to 47% with other treatments. In mutant strains, all compounds presented similar results. Thus, two components of the class hydroxycinnamic acid, caffeic acid and caffeic acid phenethyl ester (CAPE) were tested comparatively. Both acted as an antioxidant in S. cerevisiae, however CAPE was the most toxic substance promoting the most significant increase on reduced glutathione levels among hydroxycinnamic derivatives. 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dc.title.por.fl_str_mv Estudo do potencial antioxidante de diferentes classes de moléculas isoladas de mel e própolis em células de Saccharomyces cerevisiae
dc.title.alternative.eng.fl_str_mv Study of different molecules classes isolated from honey and propolis antioxidant activity in Saccharomyces cerevisiae cells
title Estudo do potencial antioxidante de diferentes classes de moléculas isoladas de mel e própolis em células de Saccharomyces cerevisiae
spellingShingle Estudo do potencial antioxidante de diferentes classes de moléculas isoladas de mel e própolis em células de Saccharomyces cerevisiae
Prudêncio, Edlene Ribeiro
Saccharomyces cerevisiae
phenolic compounds
oxidative stress
Saccharomyces cerevisiae
compostos fenólicos
estresse oxidativo
Química
title_short Estudo do potencial antioxidante de diferentes classes de moléculas isoladas de mel e própolis em células de Saccharomyces cerevisiae
title_full Estudo do potencial antioxidante de diferentes classes de moléculas isoladas de mel e própolis em células de Saccharomyces cerevisiae
title_fullStr Estudo do potencial antioxidante de diferentes classes de moléculas isoladas de mel e própolis em células de Saccharomyces cerevisiae
title_full_unstemmed Estudo do potencial antioxidante de diferentes classes de moléculas isoladas de mel e própolis em células de Saccharomyces cerevisiae
title_sort Estudo do potencial antioxidante de diferentes classes de moléculas isoladas de mel e própolis em células de Saccharomyces cerevisiae
author Prudêncio, Edlene Ribeiro
author_facet Prudêncio, Edlene Ribeiro
author_role author
dc.contributor.author.fl_str_mv Prudêncio, Edlene Ribeiro
dc.contributor.advisor1.fl_str_mv Riger, Cristiano Jorge
dc.contributor.advisor1ID.fl_str_mv 3009627700
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/8756160468801705
dc.contributor.advisor-co1.fl_str_mv Salles, Cristiane Martins Cardoso de
dc.contributor.advisor-co1ID.fl_str_mv 3539928790
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/3610279707231709
dc.contributor.referee1.fl_str_mv Barbosa, Maria Ivone Martins Jacintho
dc.contributor.referee2.fl_str_mv Pereira, Marcos Dias
dc.contributor.authorID.fl_str_mv 133.663.677-76
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/4073001345884045
contributor_str_mv Riger, Cristiano Jorge
Salles, Cristiane Martins Cardoso de
Barbosa, Maria Ivone Martins Jacintho
Pereira, Marcos Dias
dc.subject.eng.fl_str_mv Saccharomyces cerevisiae
phenolic compounds
oxidative stress
topic Saccharomyces cerevisiae
phenolic compounds
oxidative stress
Saccharomyces cerevisiae
compostos fenólicos
estresse oxidativo
Química
dc.subject.por.fl_str_mv Saccharomyces cerevisiae
compostos fenólicos
estresse oxidativo
dc.subject.cnpq.fl_str_mv Química
description O mel e a própolis são produzidos por abelhas, a partir da coleta de néctar e seiva das plantas. No processo, os compostos fenólicos oriundos do metabolismo secundário dos vegetais são incorporados aos produtos mencionados. Amostras de mel e própolis de diferentes regiões foram caracterizadas e o conteúdo de compostos fenólicos pode ser dividido em: derivados do ácido hidroxibenzoico, derivados do ácido hidroxicinâmico, flavonoides e flavonoides glicosilados. Na literatura, valores de atividade antioxidante in vitro descritos divergem consideravelmente entre os autores. Além disso, os métodos in vitro (DPPH, ABTS, FRAPP, dentre outros) não representam condições fisiológicas como concentração de substratos e interação de metabólitos. A levedura Saccharomyces cerevisie é uma ferramenta para ensaio biológico, uma vez que apresenta elevada semelhança com células de mamíferos superiores no sistema de defesa antioxidante. O objetivo deste trabalho foi comparar o potencial antioxidante de diferentes classes de compostos fenólicos, utilizando os representantes morina, rutina, ácido siríngico e clorogênico em cepas controle (BY4741) e mutantes (sod1 e gsh1) de S. cerevisiae. Foram avaliados toxidez dose-dependente, tolerância ao estresse e peroxidação lipídica. Todos os compostos fenólicos testados foram efetivos em reduzir danos oxidativos intracelulares, com destaque para o ácido clorogênico na cepa controle. Quando comparado às células estressadas, este promoveu aumentos de 75% de sobrevivência, contra 57% em média dos demais tratamentos; e diminuição de 60% em níveis de peroxidação lipídica, contra redução próxima a 47% dos demais tratamentos. Nas cepas mutantes, todas as substâncias tiveram resultados semelhantes entre si. Desta forma, outros dois componentes da classe do ácido hidroxicinâmico, ácido cafeico e éster fenetílico do ácido cafeico (CAPE), foram testados comparativamente. Ambos atuaram como antioxidante em S. cerevisiae, entretanto CAPE foi a substância mais tóxica e também a que promoveu aumento mais significativo de glutationa reduzida dentre os derivados hidroxicinâmicos. Esse resultado corrobora com dados de estudos que apontam que a atividade protetora dos compostos fenólicos está relacionada a ativação do sistema antioxidante por ação xenobiótica dessas substâncias
publishDate 2016
dc.date.issued.fl_str_mv 2016-08-04
dc.date.accessioned.fl_str_mv 2023-12-22T03:03:58Z
dc.date.available.fl_str_mv 2023-12-22T03:03:58Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv PRUDÊNCIO, Edlene Ribeiro. Estudo do potencial antioxidante de diferentes classes de moléculas isoladas de mel e própolis em células de Saccharomyces cerevisiae. 2016. 74 f. Dissertação (Mestrado em Química) - Instituto de Ciências Exatas, Universidade Federal Rural do Rio de Janeiro, Seropédica - RJ, 2016.
dc.identifier.uri.fl_str_mv https://rima.ufrrj.br/jspui/handle/20.500.14407/14648
identifier_str_mv PRUDÊNCIO, Edlene Ribeiro. Estudo do potencial antioxidante de diferentes classes de moléculas isoladas de mel e própolis em células de Saccharomyces cerevisiae. 2016. 74 f. Dissertação (Mestrado em Química) - Instituto de Ciências Exatas, Universidade Federal Rural do Rio de Janeiro, Seropédica - RJ, 2016.
url https://rima.ufrrj.br/jspui/handle/20.500.14407/14648
dc.language.iso.fl_str_mv por
language por
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