Caracterização bioquímica de proteases digestivas em larvas de Alphitobius diaperinus (Coleoptera: Tenebrionidae)

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Autor(a) principal: Cruz, Wellington Oliveira da
Data de Publicação: 2019
Tipo de documento: Tese
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
Texto Completo: https://rima.ufrrj.br/jspui/handle/20.500.14407/10195
Resumo: O Alphitobius diaperinus é considerado uma praga importante em ambiente aviário. O estudo do seu comportamento, hábitos alimentares e enzimas digestivas, podem indicar alvos importantes no seu controle. O controle do A. diaperinus é considerado difícil, seus inimigos naturais são pouco conhecidos e sendo o uso de inseticidas químico o principal método de controle. Proteases digestivas de insetos catalisam a produção de peptídeos e aminoácidos a partir de uma dieta protéica e são encontradas na região do intestino médio dos insetos. As atividades proteolíticas no trato digestivo das larvas e adultos de A. diperinus foram determinadas utilizando azocaseína e substratos especificos após alimentarem-se de diferentes dietas. O extrato bruto do homogeneizado do trato digestivo das larvas A. diaperinus mostrou atividade sobre azocaseína e Abz-Phe-Arg-MCA e foi inibida em mais de 50% pelo inibidor da cisteíno protease E-64 e foi ativado por DTT 2,0 mM em pH ácido. Nove bandas foram detectadas com atividade proteolítica e massa molecular na faixa 10 kDa – 100 kDa através de zimograma, utilizando gelatina 0,1% como substrato. Diferentes classes de proteases foram identificadas na presença de inibidores de proteases clássicos como E-64, iodoacetamida e ácido iodoacético, PMSF, SBTI, aprotinina e TLCK. Foi observado maior nível de atividade proteolítica em pHs entre 5 e 6. Embora tenham sido observadas combinações de proteases de serina e cisteína, as proteases ácidas apresentaram níveis mais altos de atividade proteolítica. O cálcio aumentou a atividade proteasica em concentrações maiores que 2,0 mM. Análises de nano-HPLC bidimensional e ESI-MS/MS resultaram na identificação de proteases como tripsina, catepsinas, metaloproteases, aminopeptidases, peptidases e dipeptidases. Foi observado alto potencial inibitório de diferentes séries de imidazolonas sobre atividade cisteíno proteases, a qual pode ser utilizada como ferramentas de controle do A. diaperinus.
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spelling Cruz, Wellington Oliveira daPontes, Emerson Guedes045.534.107-96Pontes, Emerson GuedesSouza, Cristiane Martins deSantos, Danielle M. P. de OliveiraMesquita, Rafael DiasSant`Anna, Carlos Mauricio075.736.047-50http://lattes.cnpq.br/53357617630289672023-12-21T18:58:57Z2023-12-21T18:58:57Z2019-08-06CRUZ, Wellington Oliveira. Caracterização bioquímica de proteases digestivas em larvas de Alphitobius diaperinus (Coleoptera: Tenebrionidae). 2019. 168 f. Tese (Doutorado em Química) - Instituto de Química, Universidade Federal Rural do Rio de Janeiro, Seropédica, 2019.https://rima.ufrrj.br/jspui/handle/20.500.14407/10195O Alphitobius diaperinus é considerado uma praga importante em ambiente aviário. O estudo do seu comportamento, hábitos alimentares e enzimas digestivas, podem indicar alvos importantes no seu controle. O controle do A. diaperinus é considerado difícil, seus inimigos naturais são pouco conhecidos e sendo o uso de inseticidas químico o principal método de controle. Proteases digestivas de insetos catalisam a produção de peptídeos e aminoácidos a partir de uma dieta protéica e são encontradas na região do intestino médio dos insetos. As atividades proteolíticas no trato digestivo das larvas e adultos de A. diperinus foram determinadas utilizando azocaseína e substratos especificos após alimentarem-se de diferentes dietas. O extrato bruto do homogeneizado do trato digestivo das larvas A. diaperinus mostrou atividade sobre azocaseína e Abz-Phe-Arg-MCA e foi inibida em mais de 50% pelo inibidor da cisteíno protease E-64 e foi ativado por DTT 2,0 mM em pH ácido. Nove bandas foram detectadas com atividade proteolítica e massa molecular na faixa 10 kDa – 100 kDa através de zimograma, utilizando gelatina 0,1% como substrato. Diferentes classes de proteases foram identificadas na presença de inibidores de proteases clássicos como E-64, iodoacetamida e ácido iodoacético, PMSF, SBTI, aprotinina e TLCK. Foi observado maior nível de atividade proteolítica em pHs entre 5 e 6. Embora tenham sido observadas combinações de proteases de serina e cisteína, as proteases ácidas apresentaram níveis mais altos de atividade proteolítica. O cálcio aumentou a atividade proteasica em concentrações maiores que 2,0 mM. Análises de nano-HPLC bidimensional e ESI-MS/MS resultaram na identificação de proteases como tripsina, catepsinas, metaloproteases, aminopeptidases, peptidases e dipeptidases. Foi observado alto potencial inibitório de diferentes séries de imidazolonas sobre atividade cisteíno proteases, a qual pode ser utilizada como ferramentas de controle do A. diaperinus.CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorAlphitobius diaperinus is considered an important issue in the avian environment. Studying your behavior, eating habits and digestive enzymes may indicate important targets in your control. Control of A. diaperinus is considered difficult, its natural enemies are little known, and the use of chemical insecticides is the main method of control. Insect digestive proteases catalyze the production of peptides and amino acids from a protein diet and they are found in the midgut region of insects. Proteolytic activities in the digestive tract of A. diperínus larvae and adults were determined using azocasein and specific substrates after feeding on different diets. The crude extract of the digestive tract of A. diaperinus larvae showed activity on azocasein and Abz-Phe-Arg-MCA. It was inhibited by more than 50% by E-64 and activated by 2.0 mM DTT at acid pH. Nine bands were detected with proteolytic activity and molecular mass in the range 10 kDa - 100 kDa through zymogram, using 0.1% gelatin as substrate. Different classes of proteases were identified in the presence of classical protease inhibitors such as E-64, iodoacetamide and iodoacetic acid, PMSF, SBTI, aprotinin and TLCK. A higher level of proteolytic activity was observed at pHs between 5 and 6. Although combinations of serine and cysteine proteases have been observed, acid proteases showed higher levels of proteolytic activity. Calcium ion increased the protease activity at concentrations greater than 2.0 mM. Two-dimensional nano-HPLC and ESI-MS / MS analyzes resulted in the identification of proteases such as tripine, cathepsins, metalloproteases, aminopeptidases, peptidases and dipeptidases. High inhibitory potential of different series of imidazolone on cysteine protease was observed, what can be used as control tools of A. diaperinus.application/pdfporUniversidade Federal Rural do Rio de JaneiroPrograma de Pós-Graduação em QuímicaUFRRJBrasilInstituto de QuímicaInsetoDietasProteasesAlphitobius diaperinusInsectDietsProteasesQuímicaCaracterização bioquímica de proteases digestivas em larvas de Alphitobius diaperinus (Coleoptera: Tenebrionidae)Biochemical caracterization of digestive proteases in Alphitobius diaperinus (Coleoptera: Tenebrionidae) larvaeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisAHMAD, Z.; SALEEMUDDIN, M.; SIDDI, M. Purification and characterization of three alkaline proteases from the gut of the larva of army worm, Spodoptera litura. Insect Biochemistry, v. 10, n. 6, p. 667–673, 1980. AHMEDANI, M. S.; HAQUE, M. I.; AFZAL, S. N.; ASLAM, M.; NAZ, S. 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dc.title.por.fl_str_mv Caracterização bioquímica de proteases digestivas em larvas de Alphitobius diaperinus (Coleoptera: Tenebrionidae)
dc.title.alternative.eng.fl_str_mv Biochemical caracterization of digestive proteases in Alphitobius diaperinus (Coleoptera: Tenebrionidae) larvae
title Caracterização bioquímica de proteases digestivas em larvas de Alphitobius diaperinus (Coleoptera: Tenebrionidae)
spellingShingle Caracterização bioquímica de proteases digestivas em larvas de Alphitobius diaperinus (Coleoptera: Tenebrionidae)
Cruz, Wellington Oliveira da
Inseto
Dietas
Proteases
Alphitobius diaperinus
Insect
Diets
Proteases
Química
title_short Caracterização bioquímica de proteases digestivas em larvas de Alphitobius diaperinus (Coleoptera: Tenebrionidae)
title_full Caracterização bioquímica de proteases digestivas em larvas de Alphitobius diaperinus (Coleoptera: Tenebrionidae)
title_fullStr Caracterização bioquímica de proteases digestivas em larvas de Alphitobius diaperinus (Coleoptera: Tenebrionidae)
title_full_unstemmed Caracterização bioquímica de proteases digestivas em larvas de Alphitobius diaperinus (Coleoptera: Tenebrionidae)
title_sort Caracterização bioquímica de proteases digestivas em larvas de Alphitobius diaperinus (Coleoptera: Tenebrionidae)
author Cruz, Wellington Oliveira da
author_facet Cruz, Wellington Oliveira da
author_role author
dc.contributor.author.fl_str_mv Cruz, Wellington Oliveira da
dc.contributor.advisor1.fl_str_mv Pontes, Emerson Guedes
dc.contributor.advisor1ID.fl_str_mv 045.534.107-96
dc.contributor.referee1.fl_str_mv Pontes, Emerson Guedes
dc.contributor.referee2.fl_str_mv Souza, Cristiane Martins de
dc.contributor.referee3.fl_str_mv Santos, Danielle M. P. de Oliveira
dc.contributor.referee4.fl_str_mv Mesquita, Rafael Dias
dc.contributor.referee5.fl_str_mv Sant`Anna, Carlos Mauricio
dc.contributor.authorID.fl_str_mv 075.736.047-50
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/5335761763028967
contributor_str_mv Pontes, Emerson Guedes
Pontes, Emerson Guedes
Souza, Cristiane Martins de
Santos, Danielle M. P. de Oliveira
Mesquita, Rafael Dias
Sant`Anna, Carlos Mauricio
dc.subject.por.fl_str_mv Inseto
Dietas
Proteases
Alphitobius diaperinus
topic Inseto
Dietas
Proteases
Alphitobius diaperinus
Insect
Diets
Proteases
Química
dc.subject.eng.fl_str_mv Insect
Diets
Proteases
dc.subject.cnpq.fl_str_mv Química
description O Alphitobius diaperinus é considerado uma praga importante em ambiente aviário. O estudo do seu comportamento, hábitos alimentares e enzimas digestivas, podem indicar alvos importantes no seu controle. O controle do A. diaperinus é considerado difícil, seus inimigos naturais são pouco conhecidos e sendo o uso de inseticidas químico o principal método de controle. Proteases digestivas de insetos catalisam a produção de peptídeos e aminoácidos a partir de uma dieta protéica e são encontradas na região do intestino médio dos insetos. As atividades proteolíticas no trato digestivo das larvas e adultos de A. diperinus foram determinadas utilizando azocaseína e substratos especificos após alimentarem-se de diferentes dietas. O extrato bruto do homogeneizado do trato digestivo das larvas A. diaperinus mostrou atividade sobre azocaseína e Abz-Phe-Arg-MCA e foi inibida em mais de 50% pelo inibidor da cisteíno protease E-64 e foi ativado por DTT 2,0 mM em pH ácido. Nove bandas foram detectadas com atividade proteolítica e massa molecular na faixa 10 kDa – 100 kDa através de zimograma, utilizando gelatina 0,1% como substrato. Diferentes classes de proteases foram identificadas na presença de inibidores de proteases clássicos como E-64, iodoacetamida e ácido iodoacético, PMSF, SBTI, aprotinina e TLCK. Foi observado maior nível de atividade proteolítica em pHs entre 5 e 6. Embora tenham sido observadas combinações de proteases de serina e cisteína, as proteases ácidas apresentaram níveis mais altos de atividade proteolítica. O cálcio aumentou a atividade proteasica em concentrações maiores que 2,0 mM. Análises de nano-HPLC bidimensional e ESI-MS/MS resultaram na identificação de proteases como tripsina, catepsinas, metaloproteases, aminopeptidases, peptidases e dipeptidases. Foi observado alto potencial inibitório de diferentes séries de imidazolonas sobre atividade cisteíno proteases, a qual pode ser utilizada como ferramentas de controle do A. diaperinus.
publishDate 2019
dc.date.issued.fl_str_mv 2019-08-06
dc.date.accessioned.fl_str_mv 2023-12-21T18:58:57Z
dc.date.available.fl_str_mv 2023-12-21T18:58:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv CRUZ, Wellington Oliveira. Caracterização bioquímica de proteases digestivas em larvas de Alphitobius diaperinus (Coleoptera: Tenebrionidae). 2019. 168 f. Tese (Doutorado em Química) - Instituto de Química, Universidade Federal Rural do Rio de Janeiro, Seropédica, 2019.
dc.identifier.uri.fl_str_mv https://rima.ufrrj.br/jspui/handle/20.500.14407/10195
identifier_str_mv CRUZ, Wellington Oliveira. Caracterização bioquímica de proteases digestivas em larvas de Alphitobius diaperinus (Coleoptera: Tenebrionidae). 2019. 168 f. Tese (Doutorado em Química) - Instituto de Química, Universidade Federal Rural do Rio de Janeiro, Seropédica, 2019.
url https://rima.ufrrj.br/jspui/handle/20.500.14407/10195
dc.language.iso.fl_str_mv por
language por
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