Efeito da superexpress?o do fator de transcri??o OsDof25 sobre a efici?ncia de absor??o de nitrog?nio em Orysa sativa L.
Autor(a) principal: | |
---|---|
Data de Publicação: | 2012 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFRRJ |
Texto Completo: | https://tede.ufrrj.br/jspui/handle/jspui/1692 |
Resumo: | Nitrogen is one of the nutrient elements most limiting for plant growth. Thus, increasing plant nitrogen usage efficiency (NUE) is an essential factor for sustainable agriculture, leading to an increased food production with less fertilizer input and less environment impact. The aim of this study was to evaluate the effect of OsDof25 overexpression on N-NO3- and N-NH4+ uptake. In transgenic rice plants, OsDof25 was expressed under control of maize ubiquitin promoter (UBIL:OsDof25:3xHA). Two experiments were conducted: one to evaluate the kinetic parameters Vm?x and KM, and another to analyze the expression level of nitrate (NRT2.1~2.2 and NAR) and ammonium transporters (AMT1.1~1.3), both under high and low NO3- and NH4+ supply. The untransformed plants showed higher growth that transformed lineages. The L1 and L2 showed a lower value of the KM in the resupply treatment of 0.2 mM N-NO3-. In the resupply with 0.2 mM N-NH4 + the L4 showed higher Vmax and L1 lower KM. There were no large variations in uptake kinetics between the transformed and untransformed plants. At the root the NRT2 showed low expression in lineages L1 and L4, when under constant supply of N-NO3-, in contrast, in the treatment under resupply with 0.2 mM N-NO3-was increased expression of OsNTR2.1 ~ 2.2, and NAR in both transformed lineages, but in the root the concentration of NO3- was opposed to the expression of NRT2 and NAR, in both conditions. In the leaves, the line L4 showed high expression of the transporter OsNRT2.1 with the resupply of 0.2 and 2.0 mM N-NO3-. In plants grown under constant supply of N-NH4+, L1 showed lower expression of AMT1 in the root compared to L4 and untransformed plants. When subjected to nitrogen deficiency, there was an increased expression of the OsAMT1.2. However, there was no correlation between N transporter expression levels and NO3- and NH4+ content in the transformed plants, indicating a possible change in enzyme activity and reduction or assimilation of N in these plants. The transformed plants when subjected to resupply with low levels of nitrate and ammonium showed better response parameters Vmax and KM compared to the untransformed. In the plants transformed the resupply with nitrate at low concentration resulted in increasing the gene expression of the transporters (OsNTR2.1 ~ 2.2 and protein OsNAR2.1), and the treatment with constant supply provided greatest nitrate accumulation in these plants. The results of both kinetic parameters and accumulation of fresh matter suggest that plants transformed for the expression of the OsDof25 presented highest tolerance to nutritional stress. |
id |
UFRRJ-1_89eb8431b69fb92280afbc13c0a4fec0 |
---|---|
oai_identifier_str |
oai:localhost:jspui/1692 |
network_acronym_str |
UFRRJ-1 |
network_name_str |
Biblioteca Digital de Teses e Dissertações da UFRRJ |
repository_id_str |
|
spelling |
Souza, Sonia Regina de003.927-487-03Santos, Leandro Azevedo983.907.835-68Souza., Sonia Regina deFerraz J?nior, Altamiro Souza de Lima |Cabral, Luiz Mors077.966.316-00http://lattes.cnpq.br/6396561004465081Silva, Renata Aparecida Costa2017-05-26T20:35:35Z2012-02-15SILVA, Renata Aparecida Costa. Efeito da superexpress?o do fator de transcri??o OsDof25 sobre a efici?ncia de absor??o de nitrog?nio em Orysa sativa L. 2012. 52 f. Disserta??o (Mestrado em Agronomia - Ci?ncia do Solo). Instituto de Agronomia, Departamento de Solos, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2012.https://tede.ufrrj.br/jspui/handle/jspui/1692Nitrogen is one of the nutrient elements most limiting for plant growth. Thus, increasing plant nitrogen usage efficiency (NUE) is an essential factor for sustainable agriculture, leading to an increased food production with less fertilizer input and less environment impact. The aim of this study was to evaluate the effect of OsDof25 overexpression on N-NO3- and N-NH4+ uptake. In transgenic rice plants, OsDof25 was expressed under control of maize ubiquitin promoter (UBIL:OsDof25:3xHA). Two experiments were conducted: one to evaluate the kinetic parameters Vm?x and KM, and another to analyze the expression level of nitrate (NRT2.1~2.2 and NAR) and ammonium transporters (AMT1.1~1.3), both under high and low NO3- and NH4+ supply. The untransformed plants showed higher growth that transformed lineages. The L1 and L2 showed a lower value of the KM in the resupply treatment of 0.2 mM N-NO3-. In the resupply with 0.2 mM N-NH4 + the L4 showed higher Vmax and L1 lower KM. There were no large variations in uptake kinetics between the transformed and untransformed plants. At the root the NRT2 showed low expression in lineages L1 and L4, when under constant supply of N-NO3-, in contrast, in the treatment under resupply with 0.2 mM N-NO3-was increased expression of OsNTR2.1 ~ 2.2, and NAR in both transformed lineages, but in the root the concentration of NO3- was opposed to the expression of NRT2 and NAR, in both conditions. In the leaves, the line L4 showed high expression of the transporter OsNRT2.1 with the resupply of 0.2 and 2.0 mM N-NO3-. In plants grown under constant supply of N-NH4+, L1 showed lower expression of AMT1 in the root compared to L4 and untransformed plants. When subjected to nitrogen deficiency, there was an increased expression of the OsAMT1.2. However, there was no correlation between N transporter expression levels and NO3- and NH4+ content in the transformed plants, indicating a possible change in enzyme activity and reduction or assimilation of N in these plants. The transformed plants when subjected to resupply with low levels of nitrate and ammonium showed better response parameters Vmax and KM compared to the untransformed. In the plants transformed the resupply with nitrate at low concentration resulted in increasing the gene expression of the transporters (OsNTR2.1 ~ 2.2 and protein OsNAR2.1), and the treatment with constant supply provided greatest nitrate accumulation in these plants. The results of both kinetic parameters and accumulation of fresh matter suggest that plants transformed for the expression of the OsDof25 presented highest tolerance to nutritional stress.O nitrog?nio ? um dos elementos minerais que mais limita o desenvolvimento das plantas. Assim, aumentar a efici?ncia de uso de nitrog?nio (EUN) ? um fator ? essencial para uma agricultura sustent?vel, levando a um aumento da produ??o de alimentos com menor uso de insumos e menos impactos ao ambiente. Este trabalho teve por objetivo avaliar o efeito da superexpress?o do fator de transcri??o OsDof25 sobre a absor??o de nitrog?nio em duas linhagens transformadas de arroz (L1 e L4) da variedade Nipponbare comparando-as com plantas n?o transformadas (WT). Nas plantas transformadas, o OsDof25 foi expresso sob o controle do promotor da ubiquitina 1 de milho (UBIL:OsDof25:3xHA). For feitos dois experimentos: um para avaliar os par?metros cin?ticos Vm?x e KM, sob condi??es de alto e baixo suprimento de N-NO3- e N-NH4+, e outro para analisar a express?o dos transportadores de NO3- (NRT2.1~2.2 e NAR) e NH4+ (AMT1.1~1.3) tamb?m sob alto e baixo suprimento desses ?ons. As plantas n?o transformadas apresentaram maior crescimento do que as linhagens transformadas. As L1 e a L2 mostraram menor valor de KM no tratamento com ressuprimento de 0,2 mM de N-NO3-. No ressuprimento com 0,2 mM de N-NH4+ a L4 apresentou maior Vm?x e L1 menor KM, mas, n?o houve grandes varia??es nos par?metros cin?ticos de absor??o entre as plantas transformadas e n?o transformadas. Na raiz os NRT2 mostraram baixa express?o nas linhagens L1 e L4 quando submetidas ao suprimento constante de N-NO3-, em contrapartida, no tratamento sob ressuprimento com 0,2 mM de N-NO3- ocorreu aumento de express?o dos OsNTR2.1~2.2 e NAR nas duas linhagens transformadas, por?m na raiz a concentra??o de N-NO3- foi oposta a express?o dos NRT2 e NAR, em ambas as situa??es. Nas folhas, a linhagem L4 apresentou alta express?o do transportador OsNRT2.1 com o ressuprimento de 0,2 e 2,0 mM de N-NO3-. Nas plantas submetidas ao suprimento constante de N-NH4+, a L1 apresentou menor express?o dos AMT1 na raiz quando comparada a L4 e a planta n?o transformada. Quando submetida a defici?ncia de N-NH4+, a express?o do OsAMT1.2 aumentou nas ra?zes de todas as plantas. Entretanto, n?o houve correla??o positiva entre a express?o dos transportadores de N e os teores de NO3- e NH4+ nas linhagens transformadas, indicando uma poss?vel altera??o na atividade das enzimas de redu??o e ou assimila??o de N. As plantas transformadas quando submetidas ao ressuprimento com baixos teores de nitrato e am?nio apresentaram melhor resposta dos par?metros Vm?x e KM em rela??o a n?o transformadas. Nas plantas transformadas o ressuprimento com nitrato em baixa concentra??o resultou em maior express?o dos genes dos transportadores OsNTR2.1~2.2 e da prote?na OsNAR2.1 e o tratamento com suprimento constante proporcionou maior ac?mulo de nitrato nestas plantas. Os resultados tanto dos par?metros cin?ticos quanto do ac?mulo de mat?ria fresca sugerem que as plantas transformadas para express?o do OsDof25 apresentaram maior toler?ncia ao estresse nutricional.Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-05-26T20:35:35Z No. of bitstreams: 1 2012 - Renata Aparecida Costa Silva.pdf: 1687897 bytes, checksum: 4d1af8e117daa1842ae1766319ddf8d6 (MD5)Made available in DSpace on 2017-05-26T20:35:35Z (GMT). No. of bitstreams: 1 2012 - Renata Aparecida Costa Silva.pdf: 1687897 bytes, checksum: 4d1af8e117daa1842ae1766319ddf8d6 (MD5) Previous issue date: 2012-02-15CAPESapplication/pdfhttps://tede.ufrrj.br/retrieve/5023/2012%20-%20Renata%20Aparecida%20Costa%20Silva.pdf.jpghttps://tede.ufrrj.br/retrieve/19630/2012%20-%20Renata%20Aparecida%20Costa%20Silva.pdf.jpghttps://tede.ufrrj.br/retrieve/25979/2012%20-%20Renata%20Aparecida%20Costa%20Silva.pdf.jpghttps://tede.ufrrj.br/retrieve/32416/2012%20-%20Renata%20Aparecida%20Costa%20Silva.pdf.jpghttps://tede.ufrrj.br/retrieve/38804/2012%20-%20Renata%20Aparecida%20Costa%20Silva.pdf.jpghttps://tede.ufrrj.br/retrieve/45166/2012%20-%20Renata%20Aparecida%20Costa%20Silva.pdf.jpghttps://tede.ufrrj.br/retrieve/51584/2012%20-%20Renata%20Aparecida%20Costa%20Silva.pdf.jpghttps://tede.ufrrj.br/retrieve/57992/2012%20-%20Renata%20Aparecida%20Costa%20Silva.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Agronomia - Ci?ncia do SoloUFRRJBrasilInstituto de AgronomiaAmmonium transporterNitrate transporterGene expressionTransportador de am?nioTransportador de nitratoExpress?o g?nica.AgronomiaEfeito da superexpress?o do fator de transcri??o OsDof25 sobre a efici?ncia de absor??o de nitrog?nio em Orysa sativa L.Effect of superexpression of the transcription factor OsDof25 on the efficiency of nitrogen uptake in Orysa sativa L.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRRJinstname:Universidade Federal Rural do Rio de Janeiro (UFRRJ)instacron:UFRRJTEXT2012 - Renata Aparecida Costa Silva.pdf.txt2012 - Renata Aparecida Costa Silva.pdf.txttext/plain174950http://localhost:8080/tede/bitstream/jspui/1692/17/2012+-+Renata+Aparecida+Costa+Silva.pdf.txtcad01a32e162ef60ab5a5cf8b63c1fc1MD517ORIGINAL2012 - Renata Aparecida Costa Silva.pdf2012 - Renata Aparecida Costa Silva.pdfapplication/pdf1710102http://localhost:8080/tede/bitstream/jspui/1692/4/2012+-+Renata+Aparecida+Costa+Silva.pdfcfabdb287becf263dd6276fcb0c5e786MD54THUMBNAIL2012 - Renata Aparecida Costa Silva.pdf.jpg2012 - Renata Aparecida Costa Silva.pdf.jpgimage/jpeg4550http://localhost:8080/tede/bitstream/jspui/1692/18/2012+-+Renata+Aparecida+Costa+Silva.pdf.jpg33efe4efa092dd7103e08a50d5355873MD518LICENSElicense.txtlicense.txttext/plain; charset=utf-82089http://localhost:8080/tede/bitstream/jspui/1692/1/license.txt7b5ba3d2445355f386edab96125d42b7MD51jspui/16922022-07-21 13:47:24.969oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttps://tede.ufrrj.br/PUBhttps://tede.ufrrj.br/oai/requestbibliot@ufrrj.br||bibliot@ufrrj.bropendoar:2022-07-21T16:47:24Biblioteca Digital de Teses e Dissertações da UFRRJ - Universidade Federal Rural do Rio de Janeiro (UFRRJ)false |
dc.title.por.fl_str_mv |
Efeito da superexpress?o do fator de transcri??o OsDof25 sobre a efici?ncia de absor??o de nitrog?nio em Orysa sativa L. |
dc.title.alternative.eng.fl_str_mv |
Effect of superexpression of the transcription factor OsDof25 on the efficiency of nitrogen uptake in Orysa sativa L. |
title |
Efeito da superexpress?o do fator de transcri??o OsDof25 sobre a efici?ncia de absor??o de nitrog?nio em Orysa sativa L. |
spellingShingle |
Efeito da superexpress?o do fator de transcri??o OsDof25 sobre a efici?ncia de absor??o de nitrog?nio em Orysa sativa L. Silva, Renata Aparecida Costa Ammonium transporter Nitrate transporter Gene expression Transportador de am?nio Transportador de nitrato Express?o g?nica. Agronomia |
title_short |
Efeito da superexpress?o do fator de transcri??o OsDof25 sobre a efici?ncia de absor??o de nitrog?nio em Orysa sativa L. |
title_full |
Efeito da superexpress?o do fator de transcri??o OsDof25 sobre a efici?ncia de absor??o de nitrog?nio em Orysa sativa L. |
title_fullStr |
Efeito da superexpress?o do fator de transcri??o OsDof25 sobre a efici?ncia de absor??o de nitrog?nio em Orysa sativa L. |
title_full_unstemmed |
Efeito da superexpress?o do fator de transcri??o OsDof25 sobre a efici?ncia de absor??o de nitrog?nio em Orysa sativa L. |
title_sort |
Efeito da superexpress?o do fator de transcri??o OsDof25 sobre a efici?ncia de absor??o de nitrog?nio em Orysa sativa L. |
author |
Silva, Renata Aparecida Costa |
author_facet |
Silva, Renata Aparecida Costa |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Souza, Sonia Regina de |
dc.contributor.advisor1ID.fl_str_mv |
003.927-487-03 |
dc.contributor.advisor-co1.fl_str_mv |
Santos, Leandro Azevedo |
dc.contributor.advisor-co1ID.fl_str_mv |
983.907.835-68 |
dc.contributor.referee1.fl_str_mv |
Souza., Sonia Regina de |
dc.contributor.referee2.fl_str_mv |
Ferraz J?nior, Altamiro Souza de Lima | |
dc.contributor.referee3.fl_str_mv |
Cabral, Luiz Mors |
dc.contributor.authorID.fl_str_mv |
077.966.316-00 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/6396561004465081 |
dc.contributor.author.fl_str_mv |
Silva, Renata Aparecida Costa |
contributor_str_mv |
Souza, Sonia Regina de Santos, Leandro Azevedo Souza., Sonia Regina de Ferraz J?nior, Altamiro Souza de Lima | Cabral, Luiz Mors |
dc.subject.eng.fl_str_mv |
Ammonium transporter Nitrate transporter Gene expression |
topic |
Ammonium transporter Nitrate transporter Gene expression Transportador de am?nio Transportador de nitrato Express?o g?nica. Agronomia |
dc.subject.por.fl_str_mv |
Transportador de am?nio Transportador de nitrato Express?o g?nica. |
dc.subject.cnpq.fl_str_mv |
Agronomia |
description |
Nitrogen is one of the nutrient elements most limiting for plant growth. Thus, increasing plant nitrogen usage efficiency (NUE) is an essential factor for sustainable agriculture, leading to an increased food production with less fertilizer input and less environment impact. The aim of this study was to evaluate the effect of OsDof25 overexpression on N-NO3- and N-NH4+ uptake. In transgenic rice plants, OsDof25 was expressed under control of maize ubiquitin promoter (UBIL:OsDof25:3xHA). Two experiments were conducted: one to evaluate the kinetic parameters Vm?x and KM, and another to analyze the expression level of nitrate (NRT2.1~2.2 and NAR) and ammonium transporters (AMT1.1~1.3), both under high and low NO3- and NH4+ supply. The untransformed plants showed higher growth that transformed lineages. The L1 and L2 showed a lower value of the KM in the resupply treatment of 0.2 mM N-NO3-. In the resupply with 0.2 mM N-NH4 + the L4 showed higher Vmax and L1 lower KM. There were no large variations in uptake kinetics between the transformed and untransformed plants. At the root the NRT2 showed low expression in lineages L1 and L4, when under constant supply of N-NO3-, in contrast, in the treatment under resupply with 0.2 mM N-NO3-was increased expression of OsNTR2.1 ~ 2.2, and NAR in both transformed lineages, but in the root the concentration of NO3- was opposed to the expression of NRT2 and NAR, in both conditions. In the leaves, the line L4 showed high expression of the transporter OsNRT2.1 with the resupply of 0.2 and 2.0 mM N-NO3-. In plants grown under constant supply of N-NH4+, L1 showed lower expression of AMT1 in the root compared to L4 and untransformed plants. When subjected to nitrogen deficiency, there was an increased expression of the OsAMT1.2. However, there was no correlation between N transporter expression levels and NO3- and NH4+ content in the transformed plants, indicating a possible change in enzyme activity and reduction or assimilation of N in these plants. The transformed plants when subjected to resupply with low levels of nitrate and ammonium showed better response parameters Vmax and KM compared to the untransformed. In the plants transformed the resupply with nitrate at low concentration resulted in increasing the gene expression of the transporters (OsNTR2.1 ~ 2.2 and protein OsNAR2.1), and the treatment with constant supply provided greatest nitrate accumulation in these plants. The results of both kinetic parameters and accumulation of fresh matter suggest that plants transformed for the expression of the OsDof25 presented highest tolerance to nutritional stress. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-02-15 |
dc.date.accessioned.fl_str_mv |
2017-05-26T20:35:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SILVA, Renata Aparecida Costa. Efeito da superexpress?o do fator de transcri??o OsDof25 sobre a efici?ncia de absor??o de nitrog?nio em Orysa sativa L. 2012. 52 f. Disserta??o (Mestrado em Agronomia - Ci?ncia do Solo). Instituto de Agronomia, Departamento de Solos, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2012. |
dc.identifier.uri.fl_str_mv |
https://tede.ufrrj.br/jspui/handle/jspui/1692 |
identifier_str_mv |
SILVA, Renata Aparecida Costa. Efeito da superexpress?o do fator de transcri??o OsDof25 sobre a efici?ncia de absor??o de nitrog?nio em Orysa sativa L. 2012. 52 f. Disserta??o (Mestrado em Agronomia - Ci?ncia do Solo). Instituto de Agronomia, Departamento de Solos, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2012. |
url |
https://tede.ufrrj.br/jspui/handle/jspui/1692 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal Rural do Rio de Janeiro |
dc.publisher.program.fl_str_mv |
Programa de P?s-Gradua??o em Agronomia - Ci?ncia do Solo |
dc.publisher.initials.fl_str_mv |
UFRRJ |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Instituto de Agronomia |
publisher.none.fl_str_mv |
Universidade Federal Rural do Rio de Janeiro |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ instname:Universidade Federal Rural do Rio de Janeiro (UFRRJ) instacron:UFRRJ |
instname_str |
Universidade Federal Rural do Rio de Janeiro (UFRRJ) |
instacron_str |
UFRRJ |
institution |
UFRRJ |
reponame_str |
Biblioteca Digital de Teses e Dissertações da UFRRJ |
collection |
Biblioteca Digital de Teses e Dissertações da UFRRJ |
bitstream.url.fl_str_mv |
http://localhost:8080/tede/bitstream/jspui/1692/17/2012+-+Renata+Aparecida+Costa+Silva.pdf.txt http://localhost:8080/tede/bitstream/jspui/1692/4/2012+-+Renata+Aparecida+Costa+Silva.pdf http://localhost:8080/tede/bitstream/jspui/1692/18/2012+-+Renata+Aparecida+Costa+Silva.pdf.jpg http://localhost:8080/tede/bitstream/jspui/1692/1/license.txt |
bitstream.checksum.fl_str_mv |
cad01a32e162ef60ab5a5cf8b63c1fc1 cfabdb287becf263dd6276fcb0c5e786 33efe4efa092dd7103e08a50d5355873 7b5ba3d2445355f386edab96125d42b7 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da UFRRJ - Universidade Federal Rural do Rio de Janeiro (UFRRJ) |
repository.mail.fl_str_mv |
bibliot@ufrrj.br||bibliot@ufrrj.br |
_version_ |
1800313469243752448 |