Mecanismos envolvidos com as atividades antinociceptiva, antiedematogênica e antiinflamatória do flavonoide majoritário das inflorescências de Kalanchoe pinnata (Lam.) Pers. (folha da fortuna)

Detalhes bibliográficos
Autor(a) principal: Ferreira, Raquel Teixeira
Data de Publicação: 2016
Tipo de documento: Tese
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
Texto Completo: https://rima.ufrrj.br/jspui/handle/20.500.14407/10298
Resumo: Kalanchoe pinnata (KP) é popularmente utilizada para tratamento de doenças inflamatórias. Este estudo investigou o potencial antinociceptivo e anti-inflamatório do extrato aquoso das inflorescências de KP (EAFL), a fração acetato de etila (FAcOEt), a fração butanólica (FBuOH), a fração aquosa (FAq) e o flavonoide majoritário [quercetina 3-O-α-Larabinopiranosil (1→β) α-L-ramnopiranosídeo] (KPFV) de KP, assim como possíveis mecanismos de ação. Camundongos albinos, machos (25-35g) foram pré-tratados com EAFL (30-300 mg/kg, s.c.), produzindo inibição dose dependente das contorções induzidas por ácido acético (DI50 164,8 mg/kg), e a administração subcutânea de EAFL (300 mg/kg), FAcOEt (12 mg/kg) ou FBuOH (15 mg/kg) reduziu a migração leucocitária na pleurisia induzida por carragenina em camundongos em 56,1%, 47,3%, 39,6% e 43,1%, respectivamente. Em doses até 240 vezes menores que o EAFL, KPFV (0,3-3,0 mg/kg, s.c.), também foi capaz de inibir a migração leucocitária (DI50 1,78 mg/kg). No teste do edema de orelha induzido por óleo de cróton em camundongos, EAFL (3-30 mg/kg, s.c.) e KPFV (0,3-3,0 mg/kg, s.c.) apresentaram atividade antiedematogênica dose dependente (EAFL - DI50 4,3 mg/kg, KPFV - DI50 0,76 mg/kg). O tratamento com EAFL reduziu a concentração de MPO e de TNF-α no exsudato pleural obtido do teste da pleurisia induzida por carragenina, enquanto o KPFV inibiu ambas as isoformas de cicloxigenase (COX), com CI50 3,8 x 10-5M (22,1 μg/mL) para COX-1 e um máximo de inibição da COX-2 de 43,5% (CI50 >8,4 x10-5M ou 50 μg/mL). O índice de seletividade (COX-1 CI50/COX-2 CI50) foi < 0,44. Estes resultados indicam que EAFL e KPFV produziram atividade anti-inflamatória e antinociceptiva envolvendo a inibição da COX e a redução de TNF-α. Este estudo revela que o principal flavonoide presente nas inflorescências e folhas de KP possui papel importante no uso etnomedicinal desta espécie nos processos de natureza inflamatória
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spelling Ferreira, Raquel TeixeiraVanderlinde, Frederico Argollo492.958.967-34http://lattes.cnpq.br/3183716703092107Silva, Alba Cenélia Matos daMalvar, David do CarmoPinto, Marcelo AlvesCôrtes, Wellington da Silva113.952.957-94http://lattes.cnpq.br/57433216551207322023-12-21T19:00:40Z2023-12-21T19:00:40Z2016-03-30FERREIRA, Raquel Teixeira. Mecanismos envolvidos com as atividades antinociceptiva, antiedematogênica e antiinflamatória do flavonoide majoritário das inflorescências de Kalanchoe pinnata (Lam.) Pers. (Folha da fortuna). 2016. 80 f. Tese (Programa Multicêntrico de Pós Graduação em Ciências Fisiológicas) - Instituto de Ciências Biológicas, Universidade Federal Rural do Rio de Janeiro, Seropédica, 2016.https://rima.ufrrj.br/jspui/handle/20.500.14407/10298Kalanchoe pinnata (KP) é popularmente utilizada para tratamento de doenças inflamatórias. Este estudo investigou o potencial antinociceptivo e anti-inflamatório do extrato aquoso das inflorescências de KP (EAFL), a fração acetato de etila (FAcOEt), a fração butanólica (FBuOH), a fração aquosa (FAq) e o flavonoide majoritário [quercetina 3-O-α-Larabinopiranosil (1→β) α-L-ramnopiranosídeo] (KPFV) de KP, assim como possíveis mecanismos de ação. Camundongos albinos, machos (25-35g) foram pré-tratados com EAFL (30-300 mg/kg, s.c.), produzindo inibição dose dependente das contorções induzidas por ácido acético (DI50 164,8 mg/kg), e a administração subcutânea de EAFL (300 mg/kg), FAcOEt (12 mg/kg) ou FBuOH (15 mg/kg) reduziu a migração leucocitária na pleurisia induzida por carragenina em camundongos em 56,1%, 47,3%, 39,6% e 43,1%, respectivamente. Em doses até 240 vezes menores que o EAFL, KPFV (0,3-3,0 mg/kg, s.c.), também foi capaz de inibir a migração leucocitária (DI50 1,78 mg/kg). No teste do edema de orelha induzido por óleo de cróton em camundongos, EAFL (3-30 mg/kg, s.c.) e KPFV (0,3-3,0 mg/kg, s.c.) apresentaram atividade antiedematogênica dose dependente (EAFL - DI50 4,3 mg/kg, KPFV - DI50 0,76 mg/kg). O tratamento com EAFL reduziu a concentração de MPO e de TNF-α no exsudato pleural obtido do teste da pleurisia induzida por carragenina, enquanto o KPFV inibiu ambas as isoformas de cicloxigenase (COX), com CI50 3,8 x 10-5M (22,1 μg/mL) para COX-1 e um máximo de inibição da COX-2 de 43,5% (CI50 >8,4 x10-5M ou 50 μg/mL). O índice de seletividade (COX-1 CI50/COX-2 CI50) foi < 0,44. Estes resultados indicam que EAFL e KPFV produziram atividade anti-inflamatória e antinociceptiva envolvendo a inibição da COX e a redução de TNF-α. Este estudo revela que o principal flavonoide presente nas inflorescências e folhas de KP possui papel importante no uso etnomedicinal desta espécie nos processos de natureza inflamatóriaCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorKalanchoe pinnata (KP) is popularly used for treating inflammatory diseases. This study investigated the antinociceptive and anti-inflammatory potential of the KP flower aqueous extract (KPFE), the ethyl acetate (EtOAcF), butanol (BuOHF) and aqueous (AqF) fractions and the main flavonoid [quercetin 3-O-α-L-arabinopyranosyl (1→β) α-L-rhamnopyranoside] (KPFV) of KP, as well as possible mechanisms of action. Swiss albino mice, male (25-35g) were pretreated with KPFE (30-300 mg/kg, s.c.) producing dose-related inhibition of acetic acid-induced writhing (ID50 164.8 mg/kg), and the subcutaneous administration of KPFE (300 mg/kg), EtOAcF (12 mg/kg), BuOHF (15 mg/kg) or AqF (210 mg/kg) reduced leukocyte migration on carrageenan-induced pleurisy in mice at 56.1%, 47.3%, 39.6% and 43.1%, respectively. At doses of up to 240 times smaller than KPFE, KPFV (0.3-3.0 mg/kg, s.c.), also inhibited leukocyte migration (ID50 1.78 mg/kg). In croton oil-induced ear edema in mice, KPFE (3-30 mg/kg, s.c.) and KPFV (0.3-3.0 mg/kg, s.c.) presented dose-related antiedematogenic activity (KPFE - ID50 4.3 mg/kg, KPFV - ID50 0.76 mg/kg). Treatment with KPFE reduced the MPO and TNF-α concentration in the pleural exudates on carrageenaninduced pleurisy test, while KPFV inhibited both isoforms of cyclooxygenase (COX), with IC50 3.8 x 10-5 M (22.1 μg/mL) for COX-1 and a maximum COX-2 inhibition of 43.5% (IC50 >8.4 x10-5M or 50 μg/mL). The selectivity index (COX-1 IC50/COX-2 IC50) was <0.44. These results indicate that KPFE and KPFV produced anti-inflammatory and antinociceptive activity through the COX inhibition and TNF-α reduction. This study reveals that the main flavonoid in KP flowers and leaves plays an important role in the ethnomedicinal use of this species for healing inflammatory processesapplication/pdfporUniversidade Federal Rural do Rio de JaneiroPrograma Multicêntrico de Pós-Graduação em Ciências FisiológicasUFRRJBrasilInstituto de Ciências Biológicas e da SaúdeAnti-inflammatoryFlavonoid.Kalanchoe pinnataAnti-inflamatórioFlavonoideFisiologiaMecanismos envolvidos com as atividades antinociceptiva, antiedematogênica e antiinflamatória do flavonoide majoritário das inflorescências de Kalanchoe pinnata (Lam.) Pers. (folha da fortuna)Mechanisms underlying the antinociceptive, antiedematogenic, and anti-inflammatory activity of the main flavonoid from Kalanchoe pinnata flowers (Lam.) Pers. (fortune sheet)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisABU-SOUD, H. M.; HAZEN, S. L. Nitric oxide modulates the catalytic activity of myeloperoxidase. 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dc.title.por.fl_str_mv Mecanismos envolvidos com as atividades antinociceptiva, antiedematogênica e antiinflamatória do flavonoide majoritário das inflorescências de Kalanchoe pinnata (Lam.) Pers. (folha da fortuna)
dc.title.alternative.eng.fl_str_mv Mechanisms underlying the antinociceptive, antiedematogenic, and anti-inflammatory activity of the main flavonoid from Kalanchoe pinnata flowers (Lam.) Pers. (fortune sheet)
title Mecanismos envolvidos com as atividades antinociceptiva, antiedematogênica e antiinflamatória do flavonoide majoritário das inflorescências de Kalanchoe pinnata (Lam.) Pers. (folha da fortuna)
spellingShingle Mecanismos envolvidos com as atividades antinociceptiva, antiedematogênica e antiinflamatória do flavonoide majoritário das inflorescências de Kalanchoe pinnata (Lam.) Pers. (folha da fortuna)
Ferreira, Raquel Teixeira
Anti-inflammatory
Flavonoid.
Kalanchoe pinnata
Anti-inflamatório
Flavonoide
Fisiologia
title_short Mecanismos envolvidos com as atividades antinociceptiva, antiedematogênica e antiinflamatória do flavonoide majoritário das inflorescências de Kalanchoe pinnata (Lam.) Pers. (folha da fortuna)
title_full Mecanismos envolvidos com as atividades antinociceptiva, antiedematogênica e antiinflamatória do flavonoide majoritário das inflorescências de Kalanchoe pinnata (Lam.) Pers. (folha da fortuna)
title_fullStr Mecanismos envolvidos com as atividades antinociceptiva, antiedematogênica e antiinflamatória do flavonoide majoritário das inflorescências de Kalanchoe pinnata (Lam.) Pers. (folha da fortuna)
title_full_unstemmed Mecanismos envolvidos com as atividades antinociceptiva, antiedematogênica e antiinflamatória do flavonoide majoritário das inflorescências de Kalanchoe pinnata (Lam.) Pers. (folha da fortuna)
title_sort Mecanismos envolvidos com as atividades antinociceptiva, antiedematogênica e antiinflamatória do flavonoide majoritário das inflorescências de Kalanchoe pinnata (Lam.) Pers. (folha da fortuna)
author Ferreira, Raquel Teixeira
author_facet Ferreira, Raquel Teixeira
author_role author
dc.contributor.author.fl_str_mv Ferreira, Raquel Teixeira
dc.contributor.advisor1.fl_str_mv Vanderlinde, Frederico Argollo
dc.contributor.advisor1ID.fl_str_mv 492.958.967-34
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/3183716703092107
dc.contributor.referee1.fl_str_mv Silva, Alba Cenélia Matos da
dc.contributor.referee2.fl_str_mv Malvar, David do Carmo
dc.contributor.referee3.fl_str_mv Pinto, Marcelo Alves
dc.contributor.referee4.fl_str_mv Côrtes, Wellington da Silva
dc.contributor.authorID.fl_str_mv 113.952.957-94
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/5743321655120732
contributor_str_mv Vanderlinde, Frederico Argollo
Silva, Alba Cenélia Matos da
Malvar, David do Carmo
Pinto, Marcelo Alves
Côrtes, Wellington da Silva
dc.subject.eng.fl_str_mv Anti-inflammatory
Flavonoid.
topic Anti-inflammatory
Flavonoid.
Kalanchoe pinnata
Anti-inflamatório
Flavonoide
Fisiologia
dc.subject.por.fl_str_mv Kalanchoe pinnata
Anti-inflamatório
Flavonoide
dc.subject.cnpq.fl_str_mv Fisiologia
description Kalanchoe pinnata (KP) é popularmente utilizada para tratamento de doenças inflamatórias. Este estudo investigou o potencial antinociceptivo e anti-inflamatório do extrato aquoso das inflorescências de KP (EAFL), a fração acetato de etila (FAcOEt), a fração butanólica (FBuOH), a fração aquosa (FAq) e o flavonoide majoritário [quercetina 3-O-α-Larabinopiranosil (1→β) α-L-ramnopiranosídeo] (KPFV) de KP, assim como possíveis mecanismos de ação. Camundongos albinos, machos (25-35g) foram pré-tratados com EAFL (30-300 mg/kg, s.c.), produzindo inibição dose dependente das contorções induzidas por ácido acético (DI50 164,8 mg/kg), e a administração subcutânea de EAFL (300 mg/kg), FAcOEt (12 mg/kg) ou FBuOH (15 mg/kg) reduziu a migração leucocitária na pleurisia induzida por carragenina em camundongos em 56,1%, 47,3%, 39,6% e 43,1%, respectivamente. Em doses até 240 vezes menores que o EAFL, KPFV (0,3-3,0 mg/kg, s.c.), também foi capaz de inibir a migração leucocitária (DI50 1,78 mg/kg). No teste do edema de orelha induzido por óleo de cróton em camundongos, EAFL (3-30 mg/kg, s.c.) e KPFV (0,3-3,0 mg/kg, s.c.) apresentaram atividade antiedematogênica dose dependente (EAFL - DI50 4,3 mg/kg, KPFV - DI50 0,76 mg/kg). O tratamento com EAFL reduziu a concentração de MPO e de TNF-α no exsudato pleural obtido do teste da pleurisia induzida por carragenina, enquanto o KPFV inibiu ambas as isoformas de cicloxigenase (COX), com CI50 3,8 x 10-5M (22,1 μg/mL) para COX-1 e um máximo de inibição da COX-2 de 43,5% (CI50 >8,4 x10-5M ou 50 μg/mL). O índice de seletividade (COX-1 CI50/COX-2 CI50) foi < 0,44. Estes resultados indicam que EAFL e KPFV produziram atividade anti-inflamatória e antinociceptiva envolvendo a inibição da COX e a redução de TNF-α. Este estudo revela que o principal flavonoide presente nas inflorescências e folhas de KP possui papel importante no uso etnomedicinal desta espécie nos processos de natureza inflamatória
publishDate 2016
dc.date.issued.fl_str_mv 2016-03-30
dc.date.accessioned.fl_str_mv 2023-12-21T19:00:40Z
dc.date.available.fl_str_mv 2023-12-21T19:00:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv FERREIRA, Raquel Teixeira. Mecanismos envolvidos com as atividades antinociceptiva, antiedematogênica e antiinflamatória do flavonoide majoritário das inflorescências de Kalanchoe pinnata (Lam.) Pers. (Folha da fortuna). 2016. 80 f. Tese (Programa Multicêntrico de Pós Graduação em Ciências Fisiológicas) - Instituto de Ciências Biológicas, Universidade Federal Rural do Rio de Janeiro, Seropédica, 2016.
dc.identifier.uri.fl_str_mv https://rima.ufrrj.br/jspui/handle/20.500.14407/10298
identifier_str_mv FERREIRA, Raquel Teixeira. Mecanismos envolvidos com as atividades antinociceptiva, antiedematogênica e antiinflamatória do flavonoide majoritário das inflorescências de Kalanchoe pinnata (Lam.) Pers. (Folha da fortuna). 2016. 80 f. Tese (Programa Multicêntrico de Pós Graduação em Ciências Fisiológicas) - Instituto de Ciências Biológicas, Universidade Federal Rural do Rio de Janeiro, Seropédica, 2016.
url https://rima.ufrrj.br/jspui/handle/20.500.14407/10298
dc.language.iso.fl_str_mv por
language por
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