Leveduras com potencial ação descontaminante de micotoxinas e como probiótico para aplicação na produção animal

Detalhes bibliográficos
Autor(a) principal: Keller, Kelly Moura
Data de Publicação: 2012
Tipo de documento: Tese
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
Texto Completo: https://rima.ufrrj.br/jspui/handle/20.500.14407/9572
Resumo: Micotoxinas são metabólitos secundários produzidos por certas espécies de fungos filamentosos, principalmente dos gêneros Aspergillus, Penicillium e Fusarium, e que demonstram propriedades tóxicas em humanos e animais. As aflatoxinas (AFs) são micotoxinas produzidas principalmente por Aspergillus flavus e A. parasiticus, que se desenvolvem sobre diversos gêneros alimentícios. Em todo o mundo, Sacharomyces cerevisiae virou foco de inúmeros estudos, in vitro e in vivo, devido à capacidade adsorvente exibida pela parede celular desta levedura. Sua parede celular já era usada como prebiótico para melhorar o desempenho animal, e atualmente sabe-se que um dos constituintes principais de sua parede - os glucomananos - também são capazes de adsorver diversas micotoxinas como as AFs. Assim, os objetivos gerais deste trabalho foram: I) demonstrar a eficácia in vitro de produtos comerciais, a base de parede celular de levedura (PCL) para adsorver aflatoxina B1 (AFB1), sob diferentes condições; II) demonstrar in vitro o potencial de leveduras vivas (enfoque em S. cerevisiae) para exercer ação probiótica e descontaminante de AFB1 e III) demonstrar a eficácia in vivo de produto a base de PCL como aditivos anti micotoxinas (AAM) em frangos de corte intoxicados com AFB1. Estes objetivos foram desenvolvidos em três capítulos, por separado. Ambos os AAM testados no capítulo I aderiram eficientemente a AFB1, sendo recomendada a realização de ensaios in vivo confirmatórios. As cepas de S. cerevisiae isoladas no capítulo II toleraram a passagem pelas condições in vitro gastrointestinais, apresentaram uma ou mais características probióticas, e foram moderadamente capazes de adsorver altas concentrações de AFB1; e no capítulo III, a inclusão do AAM 0,2% na dieta dos frangos de corte não produziu nenhum efeito deletério significativo sobre os animais, pelo contrário, melhorou significativamente o desempenho animal em relação ao grupo de animais intoxicados e o grupo controle. Existe a clara necessidade de uma metodologia in vitro de referência, que possa ser utilizada mundialmente, possibilitando a comparação interlaboratorial de resultados. Os resultados em geral indicam que novos estudos devem ser desenvolvidos acerca do assunto, e que há uma alternativa promissora para o desenvolvimento de novos aditivos com ação probiótica e descontaminante de micotoxinas.
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spelling Keller, Kelly MouraRosa, Carlos Alberto da Rocha362.637.537-49http://lattes.cnpq.br/2073046127303600Cavaglieri, Lilia ReneéCastro, Rosane NoraDalcero, Ana MariaDogi, Cecília AnaCurvello, Fernando AugustoSouza, Miliane Moreira Soares de085.878.127-18http://lattes.cnpq.br/19397483621889592023-12-21T18:41:44Z2023-12-21T18:41:44Z2012-02-15KELLER, Kelly Moura. Leveduras com potencial ação descontaminante de micotoxinas e como probiótico para aplicação na produção animal. 2012. 146 f. Tese (Programa de Pós-Graduação em Ciências Veterinárias) - Universidade Federal Rural do Rio de Janeiro, Seropédica.https://rima.ufrrj.br/jspui/handle/20.500.14407/9572Micotoxinas são metabólitos secundários produzidos por certas espécies de fungos filamentosos, principalmente dos gêneros Aspergillus, Penicillium e Fusarium, e que demonstram propriedades tóxicas em humanos e animais. As aflatoxinas (AFs) são micotoxinas produzidas principalmente por Aspergillus flavus e A. parasiticus, que se desenvolvem sobre diversos gêneros alimentícios. Em todo o mundo, Sacharomyces cerevisiae virou foco de inúmeros estudos, in vitro e in vivo, devido à capacidade adsorvente exibida pela parede celular desta levedura. Sua parede celular já era usada como prebiótico para melhorar o desempenho animal, e atualmente sabe-se que um dos constituintes principais de sua parede - os glucomananos - também são capazes de adsorver diversas micotoxinas como as AFs. Assim, os objetivos gerais deste trabalho foram: I) demonstrar a eficácia in vitro de produtos comerciais, a base de parede celular de levedura (PCL) para adsorver aflatoxina B1 (AFB1), sob diferentes condições; II) demonstrar in vitro o potencial de leveduras vivas (enfoque em S. cerevisiae) para exercer ação probiótica e descontaminante de AFB1 e III) demonstrar a eficácia in vivo de produto a base de PCL como aditivos anti micotoxinas (AAM) em frangos de corte intoxicados com AFB1. Estes objetivos foram desenvolvidos em três capítulos, por separado. Ambos os AAM testados no capítulo I aderiram eficientemente a AFB1, sendo recomendada a realização de ensaios in vivo confirmatórios. As cepas de S. cerevisiae isoladas no capítulo II toleraram a passagem pelas condições in vitro gastrointestinais, apresentaram uma ou mais características probióticas, e foram moderadamente capazes de adsorver altas concentrações de AFB1; e no capítulo III, a inclusão do AAM 0,2% na dieta dos frangos de corte não produziu nenhum efeito deletério significativo sobre os animais, pelo contrário, melhorou significativamente o desempenho animal em relação ao grupo de animais intoxicados e o grupo controle. Existe a clara necessidade de uma metodologia in vitro de referência, que possa ser utilizada mundialmente, possibilitando a comparação interlaboratorial de resultados. Os resultados em geral indicam que novos estudos devem ser desenvolvidos acerca do assunto, e que há uma alternativa promissora para o desenvolvimento de novos aditivos com ação probiótica e descontaminante de micotoxinas.Conselho Nacional de Desenvolvimento Científico e Tecnológico, CNPq, BrasilMycotoxins are secondary metabolites produced by certain species of filamentous fungi, mainly among Aspergillus, Penicillium and Fusarium genera, which show toxic properties in humans and animals. Aflatoxins (AFs) are mycotoxins produced mainly by Aspergillus flavus and A. parasiticus, which grow on several foodstuffs. Worldwide, Saccharomyces cerevisiae has become the focus of numerous in vitro and in vivo studies due to the adsorbent ability displayed by its yeast cell wall. The cell wall has been already used as prebiotics to improve animal performance, and now it is known that one of the main components of the cell wall - the glucomannans - are also capable of adsorbing several mycotoxins such as AFs. Thus, the general objectives of this study were: i) to demonstrate the in vitro efficacy of commercial products, source of yeast cell wall (YCW), to adsorb aflatoxin B1 (AFB1) under different conditions; II) to show the potential of live yeast (focus on S. cerevisiae) to carry out probiotics and decontamination action of AFB1 in vitro and III) to demonstrate the in vivo efficacy of YCW product as anti mycotoxins additives (AAM) in broiler chickens intoxicated with AFB1. These objectives were developed in three separated chapters. Both AAM tested in Chapter I adhered efficiently to AFB1, and in vivo tests were recommended to confirmatory results. The S. cerevisiae strains isolated in Chapter II tolerate passage through the gastrointestinal conditions in vitro, had one or more benefic characteristics, and were able to adsorb high AFB1 concentrations. In Chapter III, the inclusion of AAM 0.2% in the diet of broiler chickens produced no significant deleterious effect on animals, however, significantly improved animal performance in relation to the intoxicated animals group and the control group. There is a clear need for an in vitro reference methodology, which can be used worldwide, allowing the intercomparison of results. 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Detecção de aflatoxina B1, no organismo de frangos de corte através do emprego de ensaio imunoenzimático utilizando anticorpos monoclonais (ELISA). Acta Scientiae Veterinariae, v. 30, n. 1, p. 27-30, 2002. 145 SANTIN, E.; PAULILLO, A.C.; KRABBE, E.L.; ALESSI, A.C.; POLVEIRO, W.J.C.; MAIORKA, A. Low level of aflatoxina in broiler at experimental conditions. Use of cell wall yeasts as adsorbent of aflatoxin. Archives of Veterinary Sciences, v. 8, n. 2, p. 51-55, 2003. SANTURIO, J.M. Micotoxinas e Micotoxicoses na Avicultura. Revista Brasileira de Ciência Avícola, v. 2, n. 1, p. 1-12, 2000. SCHAEFFER, J.L.; HAMILTON, P.B. Interations of mycotoxins with feed ingredients. Do safe levels exist? In: SMITH, J.E.; HENDENSON, R.S. (Eds). Mycotoxins and Animal Foods. United States: CRC Press, 1991. p. 827-843 SHOTWELL, O.L.; HESSELTINE, C.W.; STUBBLEFIELD, R.D. & SORENSON, W.G. Production of aflatoxin on rice. Applied Microbiology, v. 14, n. 3, p. 425-428, 1966. STANLEY, V.G.; WINSMAN, M.; DUNKLEY, C.; OGUNLEYE, T.; DALEY, M.; KRUEGER, W.F.; SEFTON, A.E.; HINTON, A. The impact of yeast culture residue on the suppression of dietary aflatoxin on the performance of broiler breeder hens. Journal of Applied Poultry Research, v. 13, n. 4, 533-539, 2004. UBABEF - União Brasileira de Avicultura. História da Avicultura no Brasil. 2011. Disponível em: <http://www.abef.com.br/ubabef/exibenoticiaubabef.php?notcodigo=2675> Acesso em: 10 jan. 2012 UBABEF - União Brasileira de Avicultura. Relatório Anual Ubabef 2010/2011. 2011. 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dc.title.por.fl_str_mv Leveduras com potencial ação descontaminante de micotoxinas e como probiótico para aplicação na produção animal
dc.title.alternative.eng.fl_str_mv Yeasts with mycotoxin decontaminating and probiotic potential for use in animal production
title Leveduras com potencial ação descontaminante de micotoxinas e como probiótico para aplicação na produção animal
spellingShingle Leveduras com potencial ação descontaminante de micotoxinas e como probiótico para aplicação na produção animal
Keller, Kelly Moura
Saccharomyces cerevisiae
Paredes Celulares de Leveduras
Aflatoxina B1
Saccharomyces cerevisiae
yeast cell walls
adsorption
Medicina Veterinária
title_short Leveduras com potencial ação descontaminante de micotoxinas e como probiótico para aplicação na produção animal
title_full Leveduras com potencial ação descontaminante de micotoxinas e como probiótico para aplicação na produção animal
title_fullStr Leveduras com potencial ação descontaminante de micotoxinas e como probiótico para aplicação na produção animal
title_full_unstemmed Leveduras com potencial ação descontaminante de micotoxinas e como probiótico para aplicação na produção animal
title_sort Leveduras com potencial ação descontaminante de micotoxinas e como probiótico para aplicação na produção animal
author Keller, Kelly Moura
author_facet Keller, Kelly Moura
author_role author
dc.contributor.author.fl_str_mv Keller, Kelly Moura
dc.contributor.advisor1.fl_str_mv Rosa, Carlos Alberto da Rocha
dc.contributor.advisor1ID.fl_str_mv 362.637.537-49
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/2073046127303600
dc.contributor.advisor-co1.fl_str_mv Cavaglieri, Lilia Reneé
dc.contributor.referee1.fl_str_mv Castro, Rosane Nora
dc.contributor.referee2.fl_str_mv Dalcero, Ana Maria
dc.contributor.referee3.fl_str_mv Dogi, Cecília Ana
dc.contributor.referee4.fl_str_mv Curvello, Fernando Augusto
dc.contributor.referee5.fl_str_mv Souza, Miliane Moreira Soares de
dc.contributor.authorID.fl_str_mv 085.878.127-18
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/1939748362188959
contributor_str_mv Rosa, Carlos Alberto da Rocha
Cavaglieri, Lilia Reneé
Castro, Rosane Nora
Dalcero, Ana Maria
Dogi, Cecília Ana
Curvello, Fernando Augusto
Souza, Miliane Moreira Soares de
dc.subject.por.fl_str_mv Saccharomyces cerevisiae
Paredes Celulares de Leveduras
Aflatoxina B1
topic Saccharomyces cerevisiae
Paredes Celulares de Leveduras
Aflatoxina B1
Saccharomyces cerevisiae
yeast cell walls
adsorption
Medicina Veterinária
dc.subject.eng.fl_str_mv Saccharomyces cerevisiae
yeast cell walls
adsorption
dc.subject.cnpq.fl_str_mv Medicina Veterinária
description Micotoxinas são metabólitos secundários produzidos por certas espécies de fungos filamentosos, principalmente dos gêneros Aspergillus, Penicillium e Fusarium, e que demonstram propriedades tóxicas em humanos e animais. As aflatoxinas (AFs) são micotoxinas produzidas principalmente por Aspergillus flavus e A. parasiticus, que se desenvolvem sobre diversos gêneros alimentícios. Em todo o mundo, Sacharomyces cerevisiae virou foco de inúmeros estudos, in vitro e in vivo, devido à capacidade adsorvente exibida pela parede celular desta levedura. Sua parede celular já era usada como prebiótico para melhorar o desempenho animal, e atualmente sabe-se que um dos constituintes principais de sua parede - os glucomananos - também são capazes de adsorver diversas micotoxinas como as AFs. Assim, os objetivos gerais deste trabalho foram: I) demonstrar a eficácia in vitro de produtos comerciais, a base de parede celular de levedura (PCL) para adsorver aflatoxina B1 (AFB1), sob diferentes condições; II) demonstrar in vitro o potencial de leveduras vivas (enfoque em S. cerevisiae) para exercer ação probiótica e descontaminante de AFB1 e III) demonstrar a eficácia in vivo de produto a base de PCL como aditivos anti micotoxinas (AAM) em frangos de corte intoxicados com AFB1. Estes objetivos foram desenvolvidos em três capítulos, por separado. Ambos os AAM testados no capítulo I aderiram eficientemente a AFB1, sendo recomendada a realização de ensaios in vivo confirmatórios. As cepas de S. cerevisiae isoladas no capítulo II toleraram a passagem pelas condições in vitro gastrointestinais, apresentaram uma ou mais características probióticas, e foram moderadamente capazes de adsorver altas concentrações de AFB1; e no capítulo III, a inclusão do AAM 0,2% na dieta dos frangos de corte não produziu nenhum efeito deletério significativo sobre os animais, pelo contrário, melhorou significativamente o desempenho animal em relação ao grupo de animais intoxicados e o grupo controle. Existe a clara necessidade de uma metodologia in vitro de referência, que possa ser utilizada mundialmente, possibilitando a comparação interlaboratorial de resultados. Os resultados em geral indicam que novos estudos devem ser desenvolvidos acerca do assunto, e que há uma alternativa promissora para o desenvolvimento de novos aditivos com ação probiótica e descontaminante de micotoxinas.
publishDate 2012
dc.date.issued.fl_str_mv 2012-02-15
dc.date.accessioned.fl_str_mv 2023-12-21T18:41:44Z
dc.date.available.fl_str_mv 2023-12-21T18:41:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv KELLER, Kelly Moura. Leveduras com potencial ação descontaminante de micotoxinas e como probiótico para aplicação na produção animal. 2012. 146 f. Tese (Programa de Pós-Graduação em Ciências Veterinárias) - Universidade Federal Rural do Rio de Janeiro, Seropédica.
dc.identifier.uri.fl_str_mv https://rima.ufrrj.br/jspui/handle/20.500.14407/9572
identifier_str_mv KELLER, Kelly Moura. Leveduras com potencial ação descontaminante de micotoxinas e como probiótico para aplicação na produção animal. 2012. 146 f. Tese (Programa de Pós-Graduação em Ciências Veterinárias) - Universidade Federal Rural do Rio de Janeiro, Seropédica.
url https://rima.ufrrj.br/jspui/handle/20.500.14407/9572
dc.language.iso.fl_str_mv por
language por
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